Chemical composition of volatile oils obtained from the roots, fruits and aerial parts of Glaucosciadium cordifolium (Boiss.)B.L. Burtt&P.H. Davis (Apiaceae) were analyzed using gas chromatography-flame ionization detector/mass spectrometry,simultaneously. Furthermore, antimicrobial and antioxidant activities of G. cordifolium volatile oils were investigated for possible utilization. Total of 62 volatile compounds were identified in G. cordifolium essential oils, where the main component wascharacterized as α-pinene in all parts, commonly. The other main components were β-pinene (15.7%), (Z)-β-ocimene (14%)and sabinene (7%) in the volatile oil of the aerial part; sabinene (10.1%), β-pinene (10.1%) and α-phellandrene (5.3%) in theessential oil of the fruits; hexadecane (12.2%), tetradecane (11.9%), octadecane (7.4%) in the essential oil obtained from theroot, respectively. The in vitro microdilution method was used for the antimicrobial activity testing against Salmonella typhiATCC 6539, Acinetobacter baumanii ATCC 19606, Bacillus cereus ATCC 14579, Staphylococcus aereus ATCC 6538, Listeriamonocytogenes ATCC 19115, Helicobacter pylori ATCC 43504 and Mycobacterium avium ATCC 25291. The best antimicrobialactivity of the volatile oils was against L. monocytogenes among the tested microorganisms. In addition, DPPH•-ABTS• scavenging activity was tested, none of the essential oils showed any significant antioxidant activity.