PROTECTIVE EFFECT OF NIGELLA SATIVA OIL ON CARBOPLATIN INDUCED NEPHROTOXICITY IN RATS

Omeroglu, Suna; Cetinkaya, Kadir; Erisgin, Zuleyha; Ceyhan, Müşerref Şeyma; Atasever, Melahat

PROTECTIVE EFFECT OF NIGELLA SATIVA OIL ON CARBOPLATIN INDUCED NEPHROTOXICITY IN RATS

Züleyha ERİŞGİN, (Giresun Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Giresun, Türkiye)

Melahat ATASEVER, (Giresun Üniversitesi, Tıp Fakültesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, Giresun, Türkiye)

Müşerref Şeyma CEYHAN, (Gazi Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Ankara, Türkiye)

Suna ÖMEROĞLU, (Gazi Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Ankara, Türkiye)

Kadir ÇETİNKAYA (Ankara Onkoloji Eğitim ve Araştırma Hastanesi, Ankara, Türkiye)

Afyon Kocatepe Üniversitesi Kocatepe Tıp Dergisi

5 0

Yıl: 2020 Cilt: 21 Sayı: 3 Sayfa Aralığı: 236 - 243 Metin Dili: İngilizce İndeks Tarihi: 01-12-2020

PROTECTIVE EFFECT OF NIGELLA SATIVA OIL ON CARBOPLATIN INDUCED NEPHROTOXICITY IN RATS

Öz:

OBJECTIVE: Carboplatin is a commonly used chemotherapeutic agent. Kidneys are an important organ affected by theadverse effects of chemotherapeutic agents. This study aimedto investigate the protective effect of Nigella sativa oil (NSO)against kidney damage due to carboplatin exposure.MATERIAL AND METHODS: All animals (n=24 female wistar-albino rats) were divided into four groups; 4 ml/kg salinewas intraperitoneally (i.p.) administered on day one and twoin the first group. 4 ml/kg NSO on day one and 4 ml/kg salineon day two was i.p. administered in the second group.4 ml/kgsaline on day one and 80 mg/kg carboplatin on day two wasi.p. administered in the third group.4 ml/kg NSO on day oneand 80 mg/kg carboplatin on day two was i.p. administered inthe fourth group. Rats were sacrificed at the end of day two andrenal tissues were fixed in neutral formalin. Histopathologicchanges and apoptotic index (AI) were evaluated.RESULTS: While an increase was observed in the apoptoticindex of carboplatin+saline group compared to the controlgroup, no significant differences were found in the carboplatin+saline and carboplatin+NSO group. In the histopathological evaluation, degeneration in the proximal and distal tubularepithelium and glomerular capillary glomus bodies, congestion in the vascular formations between the tubules, increase incollagen fiber density in the tunica adventitia of intraglomerular, preglomerular, intertubular and vascular formations, andsporadic basal disintegration due to Periodic Acid Schiff (PAS)reaction were observed in the carboplatin+saline group. In thecarboplatin+NSO group, degenerative changes in some areas of tubular structures continued while it was observed thatglomerular structures were more regular. It was observed thatsclerotic change was fewer in the carboplatin+NSO group thanin the carboplatin+saline group. It drew attention that basalmembranes were more regular in the carboplatin-nigella sativaoil group as a result of PAS reaction.CONCLUSIONS: NSO, is used as a spice, may have a protectiveeffect on carboplatin induced nephrotoxicity

Anahtar Kelime:

SIÇANLARDA KARBOPLATİN İLE İNDÜKLENEN NEFROTOKSİSİTE ÜZERİNE NİGELLA SATİVA YAĞININ KORUYUCU ETKİLERİ

Öz:

AMAÇ: Karboplatin yaygın kullanılan bir kemoteröpatik ajandır. Böbrekler kemoteröpatiklerin yan etkilerinden etkilenen önemli organlardır. Bu çalışmanın amacı karboplatin kullanımına bağlı oluşan böbrek hasarına karşı Nigella sativa yağının (NSY) koruyucu etkisini araştırmaktır. GEREÇ VE YÖNTEM: Tüm hayvanlar (n=24 dişi wistar-albino sıçan) 4 gruba bölündü; birinci grupta 4 ml/kg serum fizyolojik (SF) 1 ve 2.gün uygulandı. İkinci gruba ilk gün 4 ml/kg NSY ve 4 ml/kg SF 2. Gün i.p. verildi. Üçüncü gruba 1.gün 4 ml/kg SF ve 2.gün ise karboplatin 80 mg/kg i.p. uygulandı. Dördüncü gruba 1.gün NSY ve 2. gün ise karboplatin 80 mg/kg i.p. uygulandı. İkinci günün sonunda sıçanlar sakrifiye edildi ve böbrek dokuları nötral formalin ile fikse edildi. Histopatolojik değişiklikler ve apoptotik index (AI) değerlendirildi. BULGULAR: Apoptotik indexte, karboplatin+SF grubunda kontrol grubuna göre artış görülürken, karboplatin+ NSY grubu ile anlamlı bir fark görülmemiştir. Histopatolojik değerlendirmede ise; Karboplatin+SF grubunda proksimal ve distal tubul epitelinde, glomerular kapiller yumaklarında dejenerasyon, tubuller arasında bulunan vasküler oluşumlarda konjesyon, intraglomerular, periglomerular, tubuller arası ve vasküler oluşumların tunika adventisyasında kollagen lif yoğunluğunda artış, Periyodik Asit Schiff (PAS) reaksiyonu sonucu yer yer basal membran bütünlüğünün bozulduğu görülmüştür. Karboplatin+ NSY verilen grupta ise bazı alanlardaki tubul yapılarında dejeneratif değişikliklerin devam ettiği görülürken glomerul yapılarının daha düzenli olduğu gözlemlenmiştir. Karboplatin+ NSY verilen grupta karboplatin+SF verilen gruba göre sklerotik değişimlerin daha az olduğu gözlendi. PAS reaksiyon sonucu karboplatin+ NSY verilen grupta basal membranların daha düzenli bir yapıda olduğu görüldü.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Erisgin Z, Atasever M, Ceyhan M, Omeroglu S, Cetinkaya K (2020). PROTECTIVE EFFECT OF NIGELLA SATIVA OIL ON CARBOPLATIN INDUCED NEPHROTOXICITY IN RATS. , 236 - 243.
Chicago	Erisgin Zuleyha,Atasever Melahat,Ceyhan Müşerref Şeyma,Omeroglu Suna,Cetinkaya Kadir PROTECTIVE EFFECT OF NIGELLA SATIVA OIL ON CARBOPLATIN INDUCED NEPHROTOXICITY IN RATS. (2020): 236 - 243.
MLA	Erisgin Zuleyha,Atasever Melahat,Ceyhan Müşerref Şeyma,Omeroglu Suna,Cetinkaya Kadir PROTECTIVE EFFECT OF NIGELLA SATIVA OIL ON CARBOPLATIN INDUCED NEPHROTOXICITY IN RATS. , 2020, ss.236 - 243.
AMA	Erisgin Z,Atasever M,Ceyhan M,Omeroglu S,Cetinkaya K PROTECTIVE EFFECT OF NIGELLA SATIVA OIL ON CARBOPLATIN INDUCED NEPHROTOXICITY IN RATS. . 2020; 236 - 243.
Vancouver	Erisgin Z,Atasever M,Ceyhan M,Omeroglu S,Cetinkaya K PROTECTIVE EFFECT OF NIGELLA SATIVA OIL ON CARBOPLATIN INDUCED NEPHROTOXICITY IN RATS. . 2020; 236 - 243.
IEEE	Erisgin Z,Atasever M,Ceyhan M,Omeroglu S,Cetinkaya K "PROTECTIVE EFFECT OF NIGELLA SATIVA OIL ON CARBOPLATIN INDUCED NEPHROTOXICITY IN RATS." , ss.236 - 243, 2020.
ISNAD	Erisgin, Zuleyha vd. "PROTECTIVE EFFECT OF NIGELLA SATIVA OIL ON CARBOPLATIN INDUCED NEPHROTOXICITY IN RATS". (2020), 236-243.

APA	Erisgin Z, Atasever M, Ceyhan M, Omeroglu S, Cetinkaya K (2020). PROTECTIVE EFFECT OF NIGELLA SATIVA OIL ON CARBOPLATIN INDUCED NEPHROTOXICITY IN RATS. Afyon Kocatepe Üniversitesi Kocatepe Tıp Dergisi, 21(3), 236 - 243.
Chicago	Erisgin Zuleyha,Atasever Melahat,Ceyhan Müşerref Şeyma,Omeroglu Suna,Cetinkaya Kadir PROTECTIVE EFFECT OF NIGELLA SATIVA OIL ON CARBOPLATIN INDUCED NEPHROTOXICITY IN RATS. Afyon Kocatepe Üniversitesi Kocatepe Tıp Dergisi 21, no.3 (2020): 236 - 243.
MLA	Erisgin Zuleyha,Atasever Melahat,Ceyhan Müşerref Şeyma,Omeroglu Suna,Cetinkaya Kadir PROTECTIVE EFFECT OF NIGELLA SATIVA OIL ON CARBOPLATIN INDUCED NEPHROTOXICITY IN RATS. Afyon Kocatepe Üniversitesi Kocatepe Tıp Dergisi, vol.21, no.3, 2020, ss.236 - 243.
AMA	Erisgin Z,Atasever M,Ceyhan M,Omeroglu S,Cetinkaya K PROTECTIVE EFFECT OF NIGELLA SATIVA OIL ON CARBOPLATIN INDUCED NEPHROTOXICITY IN RATS. Afyon Kocatepe Üniversitesi Kocatepe Tıp Dergisi. 2020; 21(3): 236 - 243.
Vancouver	Erisgin Z,Atasever M,Ceyhan M,Omeroglu S,Cetinkaya K PROTECTIVE EFFECT OF NIGELLA SATIVA OIL ON CARBOPLATIN INDUCED NEPHROTOXICITY IN RATS. Afyon Kocatepe Üniversitesi Kocatepe Tıp Dergisi. 2020; 21(3): 236 - 243.
IEEE	Erisgin Z,Atasever M,Ceyhan M,Omeroglu S,Cetinkaya K "PROTECTIVE EFFECT OF NIGELLA SATIVA OIL ON CARBOPLATIN INDUCED NEPHROTOXICITY IN RATS." Afyon Kocatepe Üniversitesi Kocatepe Tıp Dergisi, 21, ss.236 - 243, 2020.
ISNAD	Erisgin, Zuleyha vd. "PROTECTIVE EFFECT OF NIGELLA SATIVA OIL ON CARBOPLATIN INDUCED NEPHROTOXICITY IN RATS". Afyon Kocatepe Üniversitesi Kocatepe Tıp Dergisi 21/3 (2020), 236-243.