Phylogenetic Grouping and Antimicrobial Resistance Profiles of Escherichia coli Isolated from Calves in Xinjiang, China

Li, Jie; Meng, Qingling; Cai, Kuojun; Zhong, Fagang; Han, Mengli; Wu, Tongzhong; Huang, Xin; Zhang, Xingxing; Qiao, Jun; Chen, Ying; Zhang, Ling

doi:10.9775/kvfd.2019.23046

Phylogenetic Grouping and Antimicrobial Resistance Profiles of Escherichia coli Isolated from Calves in Xinjiang, China

Xingxing ZHANG, (Shihezi University, College of Animal Science and Technology, Shihezi, Xinjiang, China)

Jie LI, (Shihezi University, College of Animal Science and Technology, Shihezi, Xinjiang, China)

Ling ZHANG, (Tarim University, College of Animal Science and Technology, , Alar, Xinjiang, China)

Jun QIAO, (State Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, Xinjiang, China)

Qingling MENG, (State Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, Xinjiang, China)

Ying CHEN, (College of Animal Science and Technology, Xinjiang Agricultural Vocational and Technical College, Changji, Xinjiang, China)

Kuojun CAI, (Urumqi Animal Disease Control and Diagnosis Center, Urumqi, Xinjiang, China)

Xin HUANG, (State Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, Xinjiang, China)

Tongzhong WU, (State Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, Xinjiang, China)

Mengli HAN, (State Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, Xinjiang, 832000, CHINA)

Fagang ZHONG, (State Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, Xinjiang, China)

Diğer Yazarlar

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

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Yıl: 2020 Cilt: 26 Sayı: 3 Sayfa Aralığı: 329 - 335 Metin Dili: İngilizce DOI: 10.9775/kvfd.2019.23046 İndeks Tarihi: 01-12-2020

Phylogenetic Grouping and Antimicrobial Resistance Profiles of Escherichia coli Isolated from Calves in Xinjiang, China

Öz:

The widespread multidrug-resistant Escherichia coli strains have caused a severe challenge to animal health and the development of breedingindustries. The purpose of this study was to investigate the phylogenetic grouping and antimicrobial resistance profiles of E. coli isolated fromdiarrheic calves in Xinjiang province, China. In this study, a total of 379 E. coli strains were isolated from 379 rectal swab samples of diarrheic calves.They were further analyzed their phylogenetic groupings by multiplex PCR, and were clustered into four phylogenetic groups, A (36.1%), B1(17.4%), B2 (15.6%), and D (30.9%). All E. coli isolates were tested for their susceptibility to 15 antimicrobial agents by Kirby-Bauer (KB) method. Theisolates showed the highest resistance rates against ampicillin (64.9%), followed by streptomycin (59.4%), tetracycline (53.8%), sulfamethoxazole/trimethoprim (50.9%), chloramphenicol (45.6%), kanamycin (44.1%) and enrofloxacin (42.0%). E. coli isolates exhibited lower resistance toceftazidime (15.0%) and polymyxin (12.6%). The resistance genes blaTEM, blaOXA, mcr-1, strA-strB, aadA, tet(A), tet(B), and tet(C) were detected in68.3% (168/246), 27.2% (67/246), 14.6% (7/48), 51.1% (115/225), 24.9% (56/225), 51.5% (105/204), 44.6% (91/204), and 7.8% (16/204) of E. coliisolates, respectively. These results demonstrate that prevalent multi-drug resistance and high level of antimicrobial resistance genes exist amongE. coli from Xinjiang diarrheic calves and pose a potential public health concern

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APA	Zhang X, Li J, Zhang L, Qiao J, Meng Q, Chen Y, Cai K, Huang X, Wu T, Han M, Zhong F (2020). Phylogenetic Grouping and Antimicrobial Resistance Profiles of Escherichia coli Isolated from Calves in Xinjiang, China. , 329 - 335. 10.9775/kvfd.2019.23046
Chicago	Zhang Xingxing,Li Jie,Zhang Ling,Qiao Jun,Meng Qingling,Chen Ying,Cai Kuojun,Huang Xin,Wu Tongzhong,Han Mengli,Zhong Fagang Phylogenetic Grouping and Antimicrobial Resistance Profiles of Escherichia coli Isolated from Calves in Xinjiang, China. (2020): 329 - 335. 10.9775/kvfd.2019.23046
MLA	Zhang Xingxing,Li Jie,Zhang Ling,Qiao Jun,Meng Qingling,Chen Ying,Cai Kuojun,Huang Xin,Wu Tongzhong,Han Mengli,Zhong Fagang Phylogenetic Grouping and Antimicrobial Resistance Profiles of Escherichia coli Isolated from Calves in Xinjiang, China. , 2020, ss.329 - 335. 10.9775/kvfd.2019.23046
AMA	Zhang X,Li J,Zhang L,Qiao J,Meng Q,Chen Y,Cai K,Huang X,Wu T,Han M,Zhong F Phylogenetic Grouping and Antimicrobial Resistance Profiles of Escherichia coli Isolated from Calves in Xinjiang, China. . 2020; 329 - 335. 10.9775/kvfd.2019.23046
Vancouver	Zhang X,Li J,Zhang L,Qiao J,Meng Q,Chen Y,Cai K,Huang X,Wu T,Han M,Zhong F Phylogenetic Grouping and Antimicrobial Resistance Profiles of Escherichia coli Isolated from Calves in Xinjiang, China. . 2020; 329 - 335. 10.9775/kvfd.2019.23046
IEEE	Zhang X,Li J,Zhang L,Qiao J,Meng Q,Chen Y,Cai K,Huang X,Wu T,Han M,Zhong F "Phylogenetic Grouping and Antimicrobial Resistance Profiles of Escherichia coli Isolated from Calves in Xinjiang, China." , ss.329 - 335, 2020. 10.9775/kvfd.2019.23046
ISNAD	Zhang, Xingxing vd. "Phylogenetic Grouping and Antimicrobial Resistance Profiles of Escherichia coli Isolated from Calves in Xinjiang, China". (2020), 329-335. https://doi.org/10.9775/kvfd.2019.23046

APA	Zhang X, Li J, Zhang L, Qiao J, Meng Q, Chen Y, Cai K, Huang X, Wu T, Han M, Zhong F (2020). Phylogenetic Grouping and Antimicrobial Resistance Profiles of Escherichia coli Isolated from Calves in Xinjiang, China. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 26(3), 329 - 335. 10.9775/kvfd.2019.23046
Chicago	Zhang Xingxing,Li Jie,Zhang Ling,Qiao Jun,Meng Qingling,Chen Ying,Cai Kuojun,Huang Xin,Wu Tongzhong,Han Mengli,Zhong Fagang Phylogenetic Grouping and Antimicrobial Resistance Profiles of Escherichia coli Isolated from Calves in Xinjiang, China. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 26, no.3 (2020): 329 - 335. 10.9775/kvfd.2019.23046
MLA	Zhang Xingxing,Li Jie,Zhang Ling,Qiao Jun,Meng Qingling,Chen Ying,Cai Kuojun,Huang Xin,Wu Tongzhong,Han Mengli,Zhong Fagang Phylogenetic Grouping and Antimicrobial Resistance Profiles of Escherichia coli Isolated from Calves in Xinjiang, China. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.26, no.3, 2020, ss.329 - 335. 10.9775/kvfd.2019.23046
AMA	Zhang X,Li J,Zhang L,Qiao J,Meng Q,Chen Y,Cai K,Huang X,Wu T,Han M,Zhong F Phylogenetic Grouping and Antimicrobial Resistance Profiles of Escherichia coli Isolated from Calves in Xinjiang, China. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2020; 26(3): 329 - 335. 10.9775/kvfd.2019.23046
Vancouver	Zhang X,Li J,Zhang L,Qiao J,Meng Q,Chen Y,Cai K,Huang X,Wu T,Han M,Zhong F Phylogenetic Grouping and Antimicrobial Resistance Profiles of Escherichia coli Isolated from Calves in Xinjiang, China. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2020; 26(3): 329 - 335. 10.9775/kvfd.2019.23046
IEEE	Zhang X,Li J,Zhang L,Qiao J,Meng Q,Chen Y,Cai K,Huang X,Wu T,Han M,Zhong F "Phylogenetic Grouping and Antimicrobial Resistance Profiles of Escherichia coli Isolated from Calves in Xinjiang, China." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 26, ss.329 - 335, 2020. 10.9775/kvfd.2019.23046
ISNAD	Zhang, Xingxing vd. "Phylogenetic Grouping and Antimicrobial Resistance Profiles of Escherichia coli Isolated from Calves in Xinjiang, China". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 26/3 (2020), 329-335. https://doi.org/10.9775/kvfd.2019.23046