Yıl: 2020 Cilt: 10 Sayı: 3 Sayfa Aralığı: 559 - 568 Metin Dili: İngilizce DOI: 10.17714/gumusfenbil.597048 İndeks Tarihi: 17-02-2021

Investigation of Antioxidant and Cytotoxic Effects of Biotechnologically Produced Carotenoids from Rhodobacter sphaeroides O.U. 001

Öz:
Carotenoids take special attention due to their strong antioxidant properties. In the present study, the antioxidant andantiproliferative effects of carotenoids extracted from Rhodobacter sphaeroides O.U. 001 were investigated. For this, R.sphaeroides O.U. 001 was first cultivated in a 5 L bioreactor. After that, the carotenoids were extracted from R.sphaeroides O.U. 001 using acetone as solvent. The effects of carotenoid extract on proliferation of vincristine andpaclitaxel resistant cells (MCF-7/Vinc, MCF-7/Pac) were investigated by MTT cytotoxicity assay. The checkerboardplate technique was performed to evaluate the interactions between carotenoid extract and cancer drugs on the cells.The antioxidant activity of the carotenoid extract was assessed by 2, 2 - diphenyl, 1- picryl hydrazyl (DPPH) radicalscavenging assay. As a result of extraction, considerable amount of carotenoid yield was obtained (80.69 mgcarotenoid/g dry cell weight). It was found that carotenoid extract had no inhibitory effects on cell proliferation andinteraction of carotenoids with anticancer drugs exhibited antagonistic effects. In addition to these, the DPPHantioxidant activity (IC50 value) of the carotenoid extract was calculated as 25 µg/mL. In conclusion, biotechnologicallyproduced bacterial carotenoids were shown to have considerable antioxidant activity without any cytotoxicity on cancercells.
Anahtar Kelime:

Rhodobacter sphaeroides O.U.001'den Biyoteknolojik Olarak Üretilen Karotenoidlerin Antioksidan ve Sitotoksik Etkilerinin Araştırılması

Öz:
Karotenoidler güçlü antioksidan özelliklerinden dolayı dikkat çekmektedirler. Bu çalışmada, Rhodobacter sphaeroides O.U. 001'den ekstrakte edilen karotenoidlerin antioksidan ve antiproliferatif etkileri araştırıldı. Bunun için, R. sphaeroides O.U. 001 önce 5 L'lik bir biyoreaktörde çoğaltıldı. Daha sonra, çözücü olarak aseton kullanılarak R. sphaeroides O.U.001'den karotenoidler ekstrakte edildi. Karotenoid ekstraktının, vinkristin ve paklitaksele dirençli hücrelerin (MCF-7/Vinc, MCF-7/Pac) proliferasyonuna etkileri MTT sitotoksisite testi ile araştırıldı. Karotenoid ekstraktı ve kanser ilaçları arasındaki etkileşimin hücreler üzerindeki etkisi dama tahtası plaka tekniği ile değerlendirildi. Karotenoid ekstraktın antioksidan aktivitesi ise 2, 2 - difenil, 1-pikril hidrazil (DPPH) radikal süpürücü aktivite tayin yöntemi ile belirlendi. Ekstraksiyon sonucunda, önemli miktarda karotenoid verimi elde edildi (80.69 mg karotenoid/g kuru hücre ağırlığı). Karotenoid ekstraktının hücre proliferasyonu üzerinde inhibitör etkisinin olmadığı ve antikanser ilaçlarla etkileşiminin antagonistik olduğu bulundu. Bunlara ek olarak, elde edilen karotenoid ekstraktının DPPH antioksidan etkisi, (IC50 değeri) 25 µg/mL olarak hesaplandı. Sonuç olarak, biyoteknolojik yolla üretilen bakteriyel karotenoidlerin, kanser hücrelerinde sitotoksik etki göstermeden önemli antioksidan aktiviteye sahip oldukları gösterilmiştir.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Kars G, DEMİREL KARS M, OBALI İ, Ceylan A, GÜNDÜZ U (2020). Investigation of Antioxidant and Cytotoxic Effects of Biotechnologically Produced Carotenoids from Rhodobacter sphaeroides O.U. 001. , 559 - 568. 10.17714/gumusfenbil.597048
Chicago Kars Gökhan,DEMİREL KARS MELTEM,OBALI İHSAN,Ceylan Ayca,GÜNDÜZ Ufuk Investigation of Antioxidant and Cytotoxic Effects of Biotechnologically Produced Carotenoids from Rhodobacter sphaeroides O.U. 001. (2020): 559 - 568. 10.17714/gumusfenbil.597048
MLA Kars Gökhan,DEMİREL KARS MELTEM,OBALI İHSAN,Ceylan Ayca,GÜNDÜZ Ufuk Investigation of Antioxidant and Cytotoxic Effects of Biotechnologically Produced Carotenoids from Rhodobacter sphaeroides O.U. 001. , 2020, ss.559 - 568. 10.17714/gumusfenbil.597048
AMA Kars G,DEMİREL KARS M,OBALI İ,Ceylan A,GÜNDÜZ U Investigation of Antioxidant and Cytotoxic Effects of Biotechnologically Produced Carotenoids from Rhodobacter sphaeroides O.U. 001. . 2020; 559 - 568. 10.17714/gumusfenbil.597048
Vancouver Kars G,DEMİREL KARS M,OBALI İ,Ceylan A,GÜNDÜZ U Investigation of Antioxidant and Cytotoxic Effects of Biotechnologically Produced Carotenoids from Rhodobacter sphaeroides O.U. 001. . 2020; 559 - 568. 10.17714/gumusfenbil.597048
IEEE Kars G,DEMİREL KARS M,OBALI İ,Ceylan A,GÜNDÜZ U "Investigation of Antioxidant and Cytotoxic Effects of Biotechnologically Produced Carotenoids from Rhodobacter sphaeroides O.U. 001." , ss.559 - 568, 2020. 10.17714/gumusfenbil.597048
ISNAD Kars, Gökhan vd. "Investigation of Antioxidant and Cytotoxic Effects of Biotechnologically Produced Carotenoids from Rhodobacter sphaeroides O.U. 001". (2020), 559-568. https://doi.org/10.17714/gumusfenbil.597048
APA Kars G, DEMİREL KARS M, OBALI İ, Ceylan A, GÜNDÜZ U (2020). Investigation of Antioxidant and Cytotoxic Effects of Biotechnologically Produced Carotenoids from Rhodobacter sphaeroides O.U. 001. Gümüşhane Üniversitesi Fen Bilimleri Dergisi, 10(3), 559 - 568. 10.17714/gumusfenbil.597048
Chicago Kars Gökhan,DEMİREL KARS MELTEM,OBALI İHSAN,Ceylan Ayca,GÜNDÜZ Ufuk Investigation of Antioxidant and Cytotoxic Effects of Biotechnologically Produced Carotenoids from Rhodobacter sphaeroides O.U. 001. Gümüşhane Üniversitesi Fen Bilimleri Dergisi 10, no.3 (2020): 559 - 568. 10.17714/gumusfenbil.597048
MLA Kars Gökhan,DEMİREL KARS MELTEM,OBALI İHSAN,Ceylan Ayca,GÜNDÜZ Ufuk Investigation of Antioxidant and Cytotoxic Effects of Biotechnologically Produced Carotenoids from Rhodobacter sphaeroides O.U. 001. Gümüşhane Üniversitesi Fen Bilimleri Dergisi, vol.10, no.3, 2020, ss.559 - 568. 10.17714/gumusfenbil.597048
AMA Kars G,DEMİREL KARS M,OBALI İ,Ceylan A,GÜNDÜZ U Investigation of Antioxidant and Cytotoxic Effects of Biotechnologically Produced Carotenoids from Rhodobacter sphaeroides O.U. 001. Gümüşhane Üniversitesi Fen Bilimleri Dergisi. 2020; 10(3): 559 - 568. 10.17714/gumusfenbil.597048
Vancouver Kars G,DEMİREL KARS M,OBALI İ,Ceylan A,GÜNDÜZ U Investigation of Antioxidant and Cytotoxic Effects of Biotechnologically Produced Carotenoids from Rhodobacter sphaeroides O.U. 001. Gümüşhane Üniversitesi Fen Bilimleri Dergisi. 2020; 10(3): 559 - 568. 10.17714/gumusfenbil.597048
IEEE Kars G,DEMİREL KARS M,OBALI İ,Ceylan A,GÜNDÜZ U "Investigation of Antioxidant and Cytotoxic Effects of Biotechnologically Produced Carotenoids from Rhodobacter sphaeroides O.U. 001." Gümüşhane Üniversitesi Fen Bilimleri Dergisi, 10, ss.559 - 568, 2020. 10.17714/gumusfenbil.597048
ISNAD Kars, Gökhan vd. "Investigation of Antioxidant and Cytotoxic Effects of Biotechnologically Produced Carotenoids from Rhodobacter sphaeroides O.U. 001". Gümüşhane Üniversitesi Fen Bilimleri Dergisi 10/3 (2020), 559-568. https://doi.org/10.17714/gumusfenbil.597048