PRELIMINARY STUDY ON DETERMINATION OF DNA DAMAGE OF Cyprinus carpio BY SINGLE CELL GEL ELECTROPHORESIS IN IŞIKLI LAKE, DENIZLI

Çavuş, Hilal; GIDIS, M�GE

PRELIMINARY STUDY ON DETERMINATION OF DNA DAMAGE OF Cyprinus carpio BY SINGLE CELL GEL ELECTROPHORESIS IN IŞIKLI LAKE, DENIZLI

Hilal ÇAVUŞ, (Kütahya Dumlupınar Üniversitesi, Fen Bilimleri Enstitüsü, Biyokimya, Kütahya, Türkiye)

Müge GİDİŞ (Kütahya Dumlupınar Üniversitesi, Fen ve Sanat Fakültesi, Biyokimya Bölümü, Kütahya, Türkiye)

Journal of scientific reports-A (Online)

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Yıl: 2020 Cilt: 0 Sayı: 45 Sayfa Aralığı: 70 - 80 Metin Dili: İngilizce İndeks Tarihi: 23-02-2021

PRELIMINARY STUDY ON DETERMINATION OF DNA DAMAGE OF Cyprinus carpio BY SINGLE CELL GEL ELECTROPHORESIS IN IŞIKLI LAKE, DENIZLI

Öz:

In this study, DNA damage was examined in blood samples taken from Cyprinus carpio betweenOctober 2019 and December 2019. The aim of this study is to determine single strand breaks in DNAby single cell gel electrophoresis (comet analysis). Different concentrations of Methyl methanesulfonate(MMS) was applied in vitro conditions and pollution of lake was evaluated on a cell basis. DNAdamage was found to be less than the concentrations of MMS applied in October 2019. In December2019, DNA damage was found to be less than on the basis of cells compared to different concentrationsof MMS. Average Arbitrary Units (AAU) was calculated. Accordingly, the samples taken in October2019 AAU values were obtained 1,4044±0,2725, after MMS application the results were between2,38±1,1637 and 2,824444±0,6556. And in December 2019, AAU values in blood samples were foundto be between 298278±0,2213, and MMS values between 2,35±0,8381 and 2,884167±0,657. Comethead and tail lengths were calculated from the comet images obtained from the comet parameters.Comet tail lengths were determined as 15.784 ± 6.97 µm in October 2019 and 10.899 ± 4.75 µm inDecember 2019. The data obtained from results, there is not much DNA damage of C. carpio in IşıklıLake. As a result Cyprinus carpio is an efficient organism to evaluate genotoxicity of environmentalpollution and the pollution in Işıklı Lake is not at critical level.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Çavuş H, GIDIS M (2020). PRELIMINARY STUDY ON DETERMINATION OF DNA DAMAGE OF Cyprinus carpio BY SINGLE CELL GEL ELECTROPHORESIS IN IŞIKLI LAKE, DENIZLI. , 70 - 80.
Chicago	Çavuş Hilal,GIDIS M�GE PRELIMINARY STUDY ON DETERMINATION OF DNA DAMAGE OF Cyprinus carpio BY SINGLE CELL GEL ELECTROPHORESIS IN IŞIKLI LAKE, DENIZLI. (2020): 70 - 80.
MLA	Çavuş Hilal,GIDIS M�GE PRELIMINARY STUDY ON DETERMINATION OF DNA DAMAGE OF Cyprinus carpio BY SINGLE CELL GEL ELECTROPHORESIS IN IŞIKLI LAKE, DENIZLI. , 2020, ss.70 - 80.
AMA	Çavuş H,GIDIS M PRELIMINARY STUDY ON DETERMINATION OF DNA DAMAGE OF Cyprinus carpio BY SINGLE CELL GEL ELECTROPHORESIS IN IŞIKLI LAKE, DENIZLI. . 2020; 70 - 80.
Vancouver	Çavuş H,GIDIS M PRELIMINARY STUDY ON DETERMINATION OF DNA DAMAGE OF Cyprinus carpio BY SINGLE CELL GEL ELECTROPHORESIS IN IŞIKLI LAKE, DENIZLI. . 2020; 70 - 80.
IEEE	Çavuş H,GIDIS M "PRELIMINARY STUDY ON DETERMINATION OF DNA DAMAGE OF Cyprinus carpio BY SINGLE CELL GEL ELECTROPHORESIS IN IŞIKLI LAKE, DENIZLI." , ss.70 - 80, 2020.
ISNAD	Çavuş, Hilal - GIDIS, M�GE. "PRELIMINARY STUDY ON DETERMINATION OF DNA DAMAGE OF Cyprinus carpio BY SINGLE CELL GEL ELECTROPHORESIS IN IŞIKLI LAKE, DENIZLI". (2020), 70-80.

APA	Çavuş H, GIDIS M (2020). PRELIMINARY STUDY ON DETERMINATION OF DNA DAMAGE OF Cyprinus carpio BY SINGLE CELL GEL ELECTROPHORESIS IN IŞIKLI LAKE, DENIZLI. Journal of scientific reports-A (Online), 0(45), 70 - 80.
Chicago	Çavuş Hilal,GIDIS M�GE PRELIMINARY STUDY ON DETERMINATION OF DNA DAMAGE OF Cyprinus carpio BY SINGLE CELL GEL ELECTROPHORESIS IN IŞIKLI LAKE, DENIZLI. Journal of scientific reports-A (Online) 0, no.45 (2020): 70 - 80.
MLA	Çavuş Hilal,GIDIS M�GE PRELIMINARY STUDY ON DETERMINATION OF DNA DAMAGE OF Cyprinus carpio BY SINGLE CELL GEL ELECTROPHORESIS IN IŞIKLI LAKE, DENIZLI. Journal of scientific reports-A (Online), vol.0, no.45, 2020, ss.70 - 80.
AMA	Çavuş H,GIDIS M PRELIMINARY STUDY ON DETERMINATION OF DNA DAMAGE OF Cyprinus carpio BY SINGLE CELL GEL ELECTROPHORESIS IN IŞIKLI LAKE, DENIZLI. Journal of scientific reports-A (Online). 2020; 0(45): 70 - 80.
Vancouver	Çavuş H,GIDIS M PRELIMINARY STUDY ON DETERMINATION OF DNA DAMAGE OF Cyprinus carpio BY SINGLE CELL GEL ELECTROPHORESIS IN IŞIKLI LAKE, DENIZLI. Journal of scientific reports-A (Online). 2020; 0(45): 70 - 80.
IEEE	Çavuş H,GIDIS M "PRELIMINARY STUDY ON DETERMINATION OF DNA DAMAGE OF Cyprinus carpio BY SINGLE CELL GEL ELECTROPHORESIS IN IŞIKLI LAKE, DENIZLI." Journal of scientific reports-A (Online), 0, ss.70 - 80, 2020.
ISNAD	Çavuş, Hilal - GIDIS, M�GE. "PRELIMINARY STUDY ON DETERMINATION OF DNA DAMAGE OF Cyprinus carpio BY SINGLE CELL GEL ELECTROPHORESIS IN IŞIKLI LAKE, DENIZLI". Journal of scientific reports-A (Online) 45 (2020), 70-80.