In this study, the internodes locations of the roots formed in cuttings were investigated. In addition, it has been investigated whether there is a difference in root formation density for grapevine types. SO4, 5BB, Lot and 8B grapevine rootstocks obtained from Tekirdağ Viticulture and Research Institute were used and the experiment was conducted in the same institute. The cuttings were planted in deep sand boxes prepared with 15 cm row spacing (RS) and intra-row spacing (IS) on March 3, 2017. After defoliation, cuttings were removed from the boxes and measured on November 15, 2017. Root measurements were made from the left, right, opposite and just below the internodes. The criteria used were the number of roots, root length, root weight and root thickness. The two-factor factorial design model was used instead of the single-factor design model, which frequently appeared in the literature. Thus, the differences between the internodes and the differences between the grapevine types could be considered as the main factors examined. In addition, the use of two-factor experimental design allowed to investigate the interaction of grapevine types and internodes. All findings were presented and interpreted. As a result there were statistical differences between the internodes and rootstocks in terms of number of roots, root length, root weight and root thickness.