Evaluation of PVA/Chitosan Cryogels as Potential Tissue Engineering Scaffolds; Synthesis, Cytotoxicity, and Genotoxicity Investigations

Alatepeli, Burcu; ceylan, seda

doi:10.18596/jotcsa.825115

Evaluation of PVA/Chitosan Cryogels as Potential Tissue Engineering Scaffolds; Synthesis, Cytotoxicity, and Genotoxicity Investigations

Seda Ceylan, (Adana AlparslanTürkeş Bilim ve Teknoloji Üniversitesi, Mühendislik Fakültesi, Biyomühendislik Bölümü, Adana, Türkiye)

Burcu Alatepeli (Çukurova Üniversitesi, Sosyal Bilimler Enstitüsü, İşletme Anabilim Dalı, Adana, Türkiye)

Journal of the Turkish Chemical Society, Section A: Chemistry

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Yıl: 2021 Cilt: 8 Sayı: 1 Sayfa Aralığı: 69 - 78 Metin Dili: İngilizce DOI: 10.18596/jotcsa.825115 İndeks Tarihi: 27-02-2021

Evaluation of PVA/Chitosan Cryogels as Potential Tissue Engineering Scaffolds; Synthesis, Cytotoxicity, and Genotoxicity Investigations

Öz:

Cryogelation has become an advantageous method to obtain macro-porous materials with welldefined, interconnected pores for tissue engineering applications. Herein, polyvinyl alcohol and chitosanpolymers (PVA-CHI) were used to produce cryogel scaffolds via cryogelation. Glutaraldehyde was used asa crosslinking agent and the effect of crosslinking amount on the properties of scaffolds investigated.Glutaraldehyde amount was divided into 5, 10, and 15% total amount of polymer concentration. Theoptimized pore morphology was obtained as a scaffold containing 5% glutaraldehyde amount. In additionto the FTIR, SEM, swelling, and degradation analyses, mechanical tests were performed to present thecharacterization properties of the cryogels. Direct and indirect cytotoxicity test and genotoxicityexperiments were performed with Mouse Embryonic Fibroblasts (MEF). In addition, cell morphologies onscaffolds were analyzed with SEM. The results showed that PVA-CHI based cryogels had no genotoxic andcytotoxic effects on MEF cells and have a potential for tissue engineering applications.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Shaltooki M, Dini G, Mehdikhani M. Fabrication of chitosan-coated porous polycaprolactone/strontiumsubstituted bioactive glass nanocomposite scaffold for bone tissue engineering. Mater Sci Eng C [Internet]. 2019;105(May):110138. Available from: https://doi.org/10.1016/j.msec.2019.110138
2. Marsich E, Bellomo F, Turco G, Travan A, Donati I, Paoletti S. Nano-composite scaffolds for bone tissue engineering containing silver nanoparticles: preparation, characterization and biological properties. J Mater Sci Mater Med. 2013;24(7):1799–807.
3. Mani MP, Jaganathan SK, Prabhakaran P, Nageswaran G, Krishnasamy NP. Electrospun polyurethane patch in combination with cedarwood and cobalt nitrate for cardiac applications. J Appl Polym Sci. 2019;136(47):48226.
4. Hutmacher DW. Scaffolds in tissue engineering bone and cartilage. 2000;21:2529–43.
5. Salgado AJ, Coutinho OP, Reis RL. Bone tissue engineering: State of the art and future trends. Macromol Biosci. 2004;4(8):743–65.
6. Lu DR, Xiao CM, Xu SJ. Starch-based completely biodegradable polymer materials. Express Polym Lett. 2009;3(6):366–75.
7. Hsieh WC, Liau JJ. Cell culture and characterization of cross-linked poly(vinyl alcohol)- g-starch 3D scaffold for tissue engineering. Carbohydr Polym [Internet]. 2013;98(1):574–80. Available from: http://dx.doi.org/10.1016/j.carbpol.2013.06.020
8. Gualandi C. Porous Polymeric Bioresorbable Scaffolds for Tissue Engineering. University Of Bologna,Italy; 2011.
9. Kemençe N, Bölgen N. Gelatin- and hydroxyapatite-based cryogels for bone tissue engineering: synthesis, characterization, in vitro and in vivo biocompatibility. J Tissue Eng Regen Med. 2017;11(1):20–33.
10. Pinto R V, Gomes PS, Fernandes MH, Costa ME V, Almeida MM. Glutaraldehyde-crosslinking chitosan scaffolds reinforced with calcium phosphate spray-dried granules for bone tissue applications. Mater Sci Eng C. 2020;109:110557.
11. Mohammadzadehmoghadam S, Dong Y. Fabrication and characterization of electrospun silk fibroin/gelatin scaffolds crosslinked with glutaraldehyde vapor. Front Mater. 2019;6:91.
12. Ruijgrok JM, De Wijn JR, Boon ME. Optimizing glutaraldehyde crosslinking of collagen: effects of time, temperature and concentration as measured by shrinkage temperature. J Mater Sci Mater Med. 1994;5(2):80–7.
13. Yar M, Gigliobianco G, Shahzadi L, Dew L, Siddiqi SA, Khan AF, et al. Production of chitosan PVA PCL hydrogels to bind heparin and induce angiogenesis. Int J Polym Mater Polym Biomater. 2016;65(9):466–76.
14. Vrana NE, Cahill PA, McGuinness GB. Endothelialization of PVA/gelatin cryogels for vascular tissue engineering: Effect of disturbed shear stress conditions. J Biomed Mater Res - Part A. 2010;94(4):1080–90.
15. Ceylan S, Göktürk D, Demir D, Damla Özdemir M, Bölgen N. Comparison of additive effects on the PVA/starch cryogels: Synthesis, characterization, cytotoxicity, and genotoxicity studies. Int J Polym Mater Polym Biomater. 2017;1–10.
16. Chhatri A, Bajpai J, Bajpai AK. Designing polysaccharide-based antibacterial biomaterials for wound healing applications. Biomatter. 2011;1(2):189–97.
17. Zargar V, Asghari M, Dashti A. A Review on Chitin and Chitosan Polymers: Structure, Chemistry, Solubility, Derivatives, and Applications. ChemBioEng Rev [Internet]. 2015;2(3):204–26. Available from: http://doi.wiley.com/10.1002/cben.201400025
18. Râpă M, Grosu E, Stoica P, Andreica M, Hetvary M. Journal of Environmental Research and Protection Polyvinyl alcohol and starch blends: properties and biodegradation behavior. J Environ Res Prot. 2014;11(1):34.
19. Gomes ME, Ribeiro AS, Malafaya PB, Reis RL, Cunha AM. A new approach based on injection moulding to produce biodegradable starch-based polymeric sca ! olds : morphology , mechanical and degradation behaviour. Biomaterials. 2001;22.
20. Silva GA, Coutinho OP, Ducheyne P, Shapiro IM, Reis RL. The effect of starch and starch-bioactive glass composite microparticles on the adhesion and expression of the osteoblastic phenotype of a bone cell line. Biomaterials. 2007;28(2):326–34.
21. Ozmen MM, Dinu MV, Dragan ES, Okay O. Preparation of macroporous acrylamide-based hydrogels: Cryogelation under isothermal conditions. J Macromol Sci Part A Pure Appl Chem. 2007;44(11):1195–202.
22. Syverud K, Pettersen SR, Draget K, ChingaCarrasco G. Controlling the elastic modulus of cellulose nanofibril hydrogels—scaffolds with potential in tissue engineering. Cellulose. 2015;22(1):473–81.
23. Uslu İ, Atakol O, Aksu ML. Preparation of PVA / Chitosan Doped with Boron Composite Fibers and Their Characterization. Hacettepe J Biol Chem [Internet]. 2008;36(2):117–22. Available from: http://www.hjbc.hacettepe.edu.tr/article/36/2/117 %5Cnhttp://gazi.academia.edu/ibrahimUSLU
24. Liang S, Liu L, Huang Q, Yam KL. Preparation of single or double-network chitosan/poly(vinyl alcohol) gel films through selectiv0ely cross-linking method. Carbohydr Polym [Internet]. 2009;77(4):718–24. Available from: http://dx.doi.org/10.1016/j.carbpol.2009.02.007
25. Hu H, Hu H, Xin JH, Chan A, He L. Glutaraldehyde-chitosan and poly (vinyl alcohol) blends, and fluorescence of their nano-silica composite films. Carbohydr Polym [Internet]. 2013;91(1):305–13. Available from: http://dx.doi.org/10.1016/j.carbpol.2012.08.038
26. Mi Zo S, Singh D, Kumar A, Cho YW, Oh TH, Han SS. Chitosan–hydroxyapatite macroporous matrix for bone tissue engineering. Curr Sci. 2012;1438–46.
27. Reilly GC, Engler AJ. Intrinsic extracellular matrix properties regulate stem cell differentiation. J Biomech [Internet]. 2010;43(1):55–62. Available from: http://dx.doi.org/10.1016/j.jbiomech.2009.09.009
28. Peng L, Zhou Y, Lu W, Zhu W, Li Y, Chen K, et al. Characterization of a novel polyvinyl alcohol/chitosan porous hydrogel combined with bone marrow mesenchymal stem cells and its application in articular cartilage repair. BMC Musculoskelet Disord. 2019;20(1):1–12.
29. Kelly CM, DeMerlis CC, Schoneker DR, Borzelleca JF. Subchronic toxicity study in rats and genotoxicity tests with polyvinyl alcohol. Food Chem Toxicol. 2003;41(5):719–27.
30. De Lima R, Feitosa L, Pereira A do ES, De Moura MR, Aouada FA, Mattoso LHC, et al. Evaluation of the genotoxicity of chitosan nanoparticles for use in food packaging films. J Food Sci. 2010;75(6).

APA	ceylan s, Alatepeli B (2021). Evaluation of PVA/Chitosan Cryogels as Potential Tissue Engineering Scaffolds; Synthesis, Cytotoxicity, and Genotoxicity Investigations. , 69 - 78. 10.18596/jotcsa.825115
Chicago	ceylan seda,Alatepeli Burcu Evaluation of PVA/Chitosan Cryogels as Potential Tissue Engineering Scaffolds; Synthesis, Cytotoxicity, and Genotoxicity Investigations. (2021): 69 - 78. 10.18596/jotcsa.825115
MLA	ceylan seda,Alatepeli Burcu Evaluation of PVA/Chitosan Cryogels as Potential Tissue Engineering Scaffolds; Synthesis, Cytotoxicity, and Genotoxicity Investigations. , 2021, ss.69 - 78. 10.18596/jotcsa.825115
AMA	ceylan s,Alatepeli B Evaluation of PVA/Chitosan Cryogels as Potential Tissue Engineering Scaffolds; Synthesis, Cytotoxicity, and Genotoxicity Investigations. . 2021; 69 - 78. 10.18596/jotcsa.825115
Vancouver	ceylan s,Alatepeli B Evaluation of PVA/Chitosan Cryogels as Potential Tissue Engineering Scaffolds; Synthesis, Cytotoxicity, and Genotoxicity Investigations. . 2021; 69 - 78. 10.18596/jotcsa.825115
IEEE	ceylan s,Alatepeli B "Evaluation of PVA/Chitosan Cryogels as Potential Tissue Engineering Scaffolds; Synthesis, Cytotoxicity, and Genotoxicity Investigations." , ss.69 - 78, 2021. 10.18596/jotcsa.825115
ISNAD	ceylan, seda - Alatepeli, Burcu. "Evaluation of PVA/Chitosan Cryogels as Potential Tissue Engineering Scaffolds; Synthesis, Cytotoxicity, and Genotoxicity Investigations". (2021), 69-78. https://doi.org/10.18596/jotcsa.825115

APA	ceylan s, Alatepeli B (2021). Evaluation of PVA/Chitosan Cryogels as Potential Tissue Engineering Scaffolds; Synthesis, Cytotoxicity, and Genotoxicity Investigations. Journal of the Turkish Chemical Society, Section A: Chemistry, 8(1), 69 - 78. 10.18596/jotcsa.825115
Chicago	ceylan seda,Alatepeli Burcu Evaluation of PVA/Chitosan Cryogels as Potential Tissue Engineering Scaffolds; Synthesis, Cytotoxicity, and Genotoxicity Investigations. Journal of the Turkish Chemical Society, Section A: Chemistry 8, no.1 (2021): 69 - 78. 10.18596/jotcsa.825115
MLA	ceylan seda,Alatepeli Burcu Evaluation of PVA/Chitosan Cryogels as Potential Tissue Engineering Scaffolds; Synthesis, Cytotoxicity, and Genotoxicity Investigations. Journal of the Turkish Chemical Society, Section A: Chemistry, vol.8, no.1, 2021, ss.69 - 78. 10.18596/jotcsa.825115
AMA	ceylan s,Alatepeli B Evaluation of PVA/Chitosan Cryogels as Potential Tissue Engineering Scaffolds; Synthesis, Cytotoxicity, and Genotoxicity Investigations. Journal of the Turkish Chemical Society, Section A: Chemistry. 2021; 8(1): 69 - 78. 10.18596/jotcsa.825115
Vancouver	ceylan s,Alatepeli B Evaluation of PVA/Chitosan Cryogels as Potential Tissue Engineering Scaffolds; Synthesis, Cytotoxicity, and Genotoxicity Investigations. Journal of the Turkish Chemical Society, Section A: Chemistry. 2021; 8(1): 69 - 78. 10.18596/jotcsa.825115
IEEE	ceylan s,Alatepeli B "Evaluation of PVA/Chitosan Cryogels as Potential Tissue Engineering Scaffolds; Synthesis, Cytotoxicity, and Genotoxicity Investigations." Journal of the Turkish Chemical Society, Section A: Chemistry, 8, ss.69 - 78, 2021. 10.18596/jotcsa.825115
ISNAD	ceylan, seda - Alatepeli, Burcu. "Evaluation of PVA/Chitosan Cryogels as Potential Tissue Engineering Scaffolds; Synthesis, Cytotoxicity, and Genotoxicity Investigations". Journal of the Turkish Chemical Society, Section A: Chemistry 8/1 (2021), 69-78. https://doi.org/10.18596/jotcsa.825115