CHARACTERIZATION OF MICROBIAL POPULATIONS OF LAKE VAN BY 16S METAGENOMICS STUDY

POYRAZ, Nilgün; MUTLU, Mehmet Burçin

doi:10.18036/estubtdc.680795

CHARACTERIZATION OF MICROBIAL POPULATIONS OF LAKE VAN BY 16S METAGENOMICS STUDY

Nilgün POYRAZ, (Kütahya Dumlupınar Üniversitesi, Fen ve Beşeri Bilimler Fakültesi, Biyoloji Bölümü, Kütahya, Türkiye)

Mehmet Burçin MUTLU (Eskişehir Teknik Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Eskişehir, Türkiye)

Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi C- yaşam Bilimleri ve Biyoteknoloji (Online)

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Yıl: 2020 Cilt: 9 Sayı: 1 Sayfa Aralığı: 80 - 88 Metin Dili: İngilizce DOI: 10.18036/estubtdc.680795 İndeks Tarihi: 11-01-2021

CHARACTERIZATION OF MICROBIAL POPULATIONS OF LAKE VAN BY 16S METAGENOMICS STUDY

Öz:

Van Lake, located in Eastern Anatolia Region, is the largest lake in Turkey. This lake features alkaline and saline soda lake.Since there are few studies focused on microbial diversity of alkaline environments, it is very important to reveal the diversityof microorganisms of Van Lake because of the wide range of biotechnological uses of these microorganisms and theirecological roles in lake ecosystem.Next generation sequencing (NGS) allow us to sequence DNA and RNA much more quickly and cheaply than the previouslyused Sanger sequencing for the rapid analysis of the composition and diversity of microbial communities in several habitats.We applied the high throughput techniques of NGS to the metagenomics study of Van Lake, by assessing its PCR amplicon of16S rDNA sequences (V3–V4 regions). The analyses revealed Proteobacteria, Actinobacteria and Verrucomicrobia phylawere most abundant. Archaea members were represented by Euryarchaeota phylum. Results concluded that Bacteria domain were dominant in Van Lake.

Anahtar Kelime:

VAN GÖLÜ MİKROBİYAL POPULASYONLARININ 16S METAGENOM ÇALIŞMASI İLE KARAKTERİZASYONU

Öz:

Doğu Anadolu Bölgesi'nde bulunan Van Gölü, Türkiye'nin en büyük gölüdür. Bu göl alkali ve tuzlu soda gölüdür. Alkali ortamların mikrobiyal çeşitliliğine odaklanan az sayıda çalışma olduğu için, bu mikroorganizmaların geniş biyoteknolojik kullanımları ve göl ekosistemindeki ekolojik rolleri nedeniyle Van Gölü'nün mikrobiyal çeşitliliğini ortaya koymak çok önemlidir. Yeni nesil dizileme (NGS), bazı habitatlarda mikrobiyal toplulukların kompozisyon ve çeşitliliğinin hızlı analizi için daha önce kullanılan Sanger dizilemesinden çok daha hızlı ve ucuz bir şekilde DNA ve RNA dizilememizi sağlar. Çalışmamızda 16S rDNA sekanslarının (V3-V4 bölgeleri) PCR amplikonlarını değerlendirerek Van Gölü’nün metagenomik analizi kapsamında, yüksek verimli NGS uygulanmıştır. Analizler Proteobacteria, Actinobacteria ve Verrucomicrobia filumlarının baskın olduğunu göstermiştir. Archaea üyeleri ise Euryarchaeota filumu tarafından temsil edilmiştir. Sonuçlar Van Gölü'nde Bacteria domaininin baskın olduğunu ortaya çıkarmıştır.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	POYRAZ N, MUTLU M (2020). CHARACTERIZATION OF MICROBIAL POPULATIONS OF LAKE VAN BY 16S METAGENOMICS STUDY. , 80 - 88. 10.18036/estubtdc.680795
Chicago	POYRAZ Nilgün,MUTLU Mehmet Burçin CHARACTERIZATION OF MICROBIAL POPULATIONS OF LAKE VAN BY 16S METAGENOMICS STUDY. (2020): 80 - 88. 10.18036/estubtdc.680795
MLA	POYRAZ Nilgün,MUTLU Mehmet Burçin CHARACTERIZATION OF MICROBIAL POPULATIONS OF LAKE VAN BY 16S METAGENOMICS STUDY. , 2020, ss.80 - 88. 10.18036/estubtdc.680795
AMA	POYRAZ N,MUTLU M CHARACTERIZATION OF MICROBIAL POPULATIONS OF LAKE VAN BY 16S METAGENOMICS STUDY. . 2020; 80 - 88. 10.18036/estubtdc.680795
Vancouver	POYRAZ N,MUTLU M CHARACTERIZATION OF MICROBIAL POPULATIONS OF LAKE VAN BY 16S METAGENOMICS STUDY. . 2020; 80 - 88. 10.18036/estubtdc.680795
IEEE	POYRAZ N,MUTLU M "CHARACTERIZATION OF MICROBIAL POPULATIONS OF LAKE VAN BY 16S METAGENOMICS STUDY." , ss.80 - 88, 2020. 10.18036/estubtdc.680795
ISNAD	POYRAZ, Nilgün - MUTLU, Mehmet Burçin. "CHARACTERIZATION OF MICROBIAL POPULATIONS OF LAKE VAN BY 16S METAGENOMICS STUDY". (2020), 80-88. https://doi.org/10.18036/estubtdc.680795

APA	POYRAZ N, MUTLU M (2020). CHARACTERIZATION OF MICROBIAL POPULATIONS OF LAKE VAN BY 16S METAGENOMICS STUDY. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi C- yaşam Bilimleri ve Biyoteknoloji (Online), 9(1), 80 - 88. 10.18036/estubtdc.680795
Chicago	POYRAZ Nilgün,MUTLU Mehmet Burçin CHARACTERIZATION OF MICROBIAL POPULATIONS OF LAKE VAN BY 16S METAGENOMICS STUDY. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi C- yaşam Bilimleri ve Biyoteknoloji (Online) 9, no.1 (2020): 80 - 88. 10.18036/estubtdc.680795
MLA	POYRAZ Nilgün,MUTLU Mehmet Burçin CHARACTERIZATION OF MICROBIAL POPULATIONS OF LAKE VAN BY 16S METAGENOMICS STUDY. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi C- yaşam Bilimleri ve Biyoteknoloji (Online), vol.9, no.1, 2020, ss.80 - 88. 10.18036/estubtdc.680795
AMA	POYRAZ N,MUTLU M CHARACTERIZATION OF MICROBIAL POPULATIONS OF LAKE VAN BY 16S METAGENOMICS STUDY. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi C- yaşam Bilimleri ve Biyoteknoloji (Online). 2020; 9(1): 80 - 88. 10.18036/estubtdc.680795
Vancouver	POYRAZ N,MUTLU M CHARACTERIZATION OF MICROBIAL POPULATIONS OF LAKE VAN BY 16S METAGENOMICS STUDY. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi C- yaşam Bilimleri ve Biyoteknoloji (Online). 2020; 9(1): 80 - 88. 10.18036/estubtdc.680795
IEEE	POYRAZ N,MUTLU M "CHARACTERIZATION OF MICROBIAL POPULATIONS OF LAKE VAN BY 16S METAGENOMICS STUDY." Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi C- yaşam Bilimleri ve Biyoteknoloji (Online), 9, ss.80 - 88, 2020. 10.18036/estubtdc.680795
ISNAD	POYRAZ, Nilgün - MUTLU, Mehmet Burçin. "CHARACTERIZATION OF MICROBIAL POPULATIONS OF LAKE VAN BY 16S METAGENOMICS STUDY". Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi C- yaşam Bilimleri ve Biyoteknoloji (Online) 9/1 (2020), 80-88. https://doi.org/10.18036/estubtdc.680795