Histopathology and Antibiotic Susceptibility of Aeromonas hydrophila Isolated from Diseased Guppy (Poecilia reticulata)
Yıl: 2020 Cilt: 13 Sayı: 1 Sayfa Aralığı: 306 - 313 Metin Dili: İngilizce DOI: 10.18185/erzifbed.544683 İndeks Tarihi: 26-01-2021
Histopathology and Antibiotic Susceptibility of Aeromonas hydrophila Isolated from Diseased Guppy (Poecilia reticulata)
Öz: This study was carried out to determine the reason of mortality in guppy (Poecilia reticulata) kept in anaquarium fish-rearing farm in Istanbul province and confirm damage caused in tissues and antibioticsusceptibility of isolated pathogen bacteria. Externally diseased guppies (n=15) showed sloughing scales,ulcerative skin lesions on the body surface and abdominal distension; internally pale liver and accumulation ofa liquid in the abdominal cavity were observed. Bacteriological inoculation from internal organs such as liver,spleen and kidney of guppies (n=15) were made onto Tryptic Soy Agar (TSA) and Brain Heart Infusion Agar(BHIA). After incubation, all isolated bacteria were identified as Aeromonas hydrophila according to theirpsychological, morphological, biochemical characteristic, and API 20E profile. Also, these isolates (n=15) weredetermined to be sensitive to chloramphenicol and florfenicol. Histopathologically, multifocal liquefactivenecrosis in the spleen, liver, kidney and heart, degeneration of tubular epithelium and necrosis in the kidney,desquamation of mucosa epithelium and necrotic gastric glands in the stomach, discharge of the white pulp inthe spleen, desquamation of the lamina propria and mucosa epithelium in the intestine, and sloughed off thenecrotic gill epithelium cells were observed.
Anahtar Kelime: Hasta Lepistes Balığından (Poecilia reticulata)İzole Edilen Aeromonas hydrophila’ nın Antibiyotik Duyarlılığı ve Histopatolojisi
Öz: Bu çalışma İstanbul ilinde bulunan bir akvaryum balığı üretim işletmesindeki hasta lepistes balıklarında görülen ölümlerin nedenini ortaya koymak ve izole edilen patojen bakterinin dokularda oluşturduğu hasarı ve antibiyotik duyarlılığını tespit etmek amacı ile yürütülmüştür. Eksternal olarak, ölmek üzere olan lepistes (Poecilia reticulata) balıklarında pullarda dökülme, vücut yüzeyinde ülseratif deri lezyonları ve abdominal genişleme görüldü; internal olarak karaciğerin renginde açılma, abdominal boşlukta sıvı birikimi tespit edildi. Karaciğer, böbrek ve dalak gibi iç organlardan Tryptic Soy Agar (TSA) and Brain Heart Infusion Agar (BHIA) besiyerlerine ekimler yapıldı. İnkubasyon sonrasında izole edilen tüm bakteriler fizyolojik, morfolojik ve biyokimyasal özellikleri ve API 20E profiline göre Aeromonas hydrophila olarak identifiye edildi. Bu izolatların (n=15) kloramfenikol ve florfenikol’e duyarlı olduğu tespit edildi. Histopatolojik olarak dalak, karaciğer, böbrek ve kalp dokusunda multifokal liquefaktif nekroz, tubuler böbrek epitelyumunda dejenerasyon ve nekroz, mide bezlerinde ve mukoza epitelyumunda dökülme, dalağın kırmızı pulpasında boşalma, bağırsağın mukoza epitelinde ve lamina propriasında dökülme yanı sıra nekrotik solungaç epitel hücrelerinde dökülme tespit edilmiştir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | ÜRKÜ Ç (2020). Histopathology and Antibiotic Susceptibility of Aeromonas hydrophila Isolated from Diseased Guppy (Poecilia reticulata). , 306 - 313. 10.18185/erzifbed.544683 |
| Chicago | ÜRKÜ Çiğdem Histopathology and Antibiotic Susceptibility of Aeromonas hydrophila Isolated from Diseased Guppy (Poecilia reticulata). (2020): 306 - 313. 10.18185/erzifbed.544683 |
| MLA | ÜRKÜ Çiğdem Histopathology and Antibiotic Susceptibility of Aeromonas hydrophila Isolated from Diseased Guppy (Poecilia reticulata). , 2020, ss.306 - 313. 10.18185/erzifbed.544683 |
| AMA | ÜRKÜ Ç Histopathology and Antibiotic Susceptibility of Aeromonas hydrophila Isolated from Diseased Guppy (Poecilia reticulata). . 2020; 306 - 313. 10.18185/erzifbed.544683 |
| Vancouver | ÜRKÜ Ç Histopathology and Antibiotic Susceptibility of Aeromonas hydrophila Isolated from Diseased Guppy (Poecilia reticulata). . 2020; 306 - 313. 10.18185/erzifbed.544683 |
| IEEE | ÜRKÜ Ç "Histopathology and Antibiotic Susceptibility of Aeromonas hydrophila Isolated from Diseased Guppy (Poecilia reticulata)." , ss.306 - 313, 2020. 10.18185/erzifbed.544683 |
| ISNAD | ÜRKÜ, Çiğdem. "Histopathology and Antibiotic Susceptibility of Aeromonas hydrophila Isolated from Diseased Guppy (Poecilia reticulata)". (2020), 306-313. https://doi.org/10.18185/erzifbed.544683 |
| APA | ÜRKÜ Ç (2020). Histopathology and Antibiotic Susceptibility of Aeromonas hydrophila Isolated from Diseased Guppy (Poecilia reticulata). Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 13(1), 306 - 313. 10.18185/erzifbed.544683 |
| Chicago | ÜRKÜ Çiğdem Histopathology and Antibiotic Susceptibility of Aeromonas hydrophila Isolated from Diseased Guppy (Poecilia reticulata). Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi 13, no.1 (2020): 306 - 313. 10.18185/erzifbed.544683 |
| MLA | ÜRKÜ Çiğdem Histopathology and Antibiotic Susceptibility of Aeromonas hydrophila Isolated from Diseased Guppy (Poecilia reticulata). Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol.13, no.1, 2020, ss.306 - 313. 10.18185/erzifbed.544683 |
| AMA | ÜRKÜ Ç Histopathology and Antibiotic Susceptibility of Aeromonas hydrophila Isolated from Diseased Guppy (Poecilia reticulata). Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2020; 13(1): 306 - 313. 10.18185/erzifbed.544683 |
| Vancouver | ÜRKÜ Ç Histopathology and Antibiotic Susceptibility of Aeromonas hydrophila Isolated from Diseased Guppy (Poecilia reticulata). Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2020; 13(1): 306 - 313. 10.18185/erzifbed.544683 |
| IEEE | ÜRKÜ Ç "Histopathology and Antibiotic Susceptibility of Aeromonas hydrophila Isolated from Diseased Guppy (Poecilia reticulata)." Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 13, ss.306 - 313, 2020. 10.18185/erzifbed.544683 |
| ISNAD | ÜRKÜ, Çiğdem. "Histopathology and Antibiotic Susceptibility of Aeromonas hydrophila Isolated from Diseased Guppy (Poecilia reticulata)". Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi 13/1 (2020), 306-313. https://doi.org/10.18185/erzifbed.544683 |
