In Vitro Anti-Helicobacter pylori and Antimycobacterial Activity Evaluation of Selected Plants From Turkey
Yıl: 2020 Cilt: 5 Sayı: 2 Sayfa Aralığı: 231 - 235 Metin Dili: İngilizce DOI: 10.35229/jaes.689341 İndeks Tarihi: 01-02-2021
In Vitro Anti-Helicobacter pylori and Antimycobacterial Activity Evaluation of Selected Plants From Turkey
Öz: In this study, the extracts of Ulmus minor Mill. subsp. minor (Ulmaceae), Lathyruspratensis L. (Leguminosae), Glaucium leiocarpum Bois. (Papaveraceae), and Echium vulgareL. (Boraginacea) were investigated for their in vitro anti-Helicobacter pylori and antimycobacterial activity.The air-dried plant material was powdered and extracted with 70% ethanol by maceration. Theextract was filtered and evaporated to dryness under the vacuum and then dissolved in a waterethanol (90:10) mixture and extracted with dichloromethane and ethyl acetate, respectively.Antibacterial activity was investigated by microdilution method against Helicobacter pyloriATCC 43504, Mycolicibacterium smegmatis ATCC 14468 and Mycobacterium avium ATCC25291.The ethyl acetate extract of U. minor showed activity with MIC of 250 µg / mL against H. pylori.The other extracts showed no or weak inhibitory activity at >2000 µg/mL concentrations againstthe tested microorganisms. In conclusion, ethyl acetate extract of U. minor may be used for thetreatment of H. pylori infections.
Anahtar Kelime: Türkiye’de Yetiştirilen Bazı Bitkilerin İn Vitro Anti-Helikobakter pilori ve Antimikobakteriyel Aktivitesi
Öz: Bu çalışmada, Ulmus minor Mill. subsp. minor (Ulmaceae), Lathyrus pratensis L. (Leguminosae), Glaucium leiocarpum Bois. (Papaveraceae) ve Echium vulgare L. (Boraginacea) ekstrelerinin antihelikobakter pilori ve antimikobakter aktiviteleri in vitro olarak incelenmiştir. Kurutulmuş bitki materyalleri toz edildikten sonra %70’lik etanolle masere edilmiştir. Ekstreler süzülerek vakum altında yoğunlaştırıldıktan sonra su-etanol (90:10) karışımında çözülerek sırasıyla diklorometan ve etilasetat ile alt ekstreleri hazırlanmıştır. Antibakteriyel aktivitesi in vitro broth mikrodilüsyon yöntemi ile Helicobacter pylori ATCC 43504, Mycolicibacterium smegmatis ATCC 14468 ve Mycobacterium avium ATCC 25291’a karşı araştırılmıştır. U. minor’un etilasetat ekstresinin MİK değeri H. pilori’ye karşı 250 µg/mL olarak kaydedilmiştir. Test edilen konsantrasyonlarda (2000 µg/mL) diğer ekstreler belirgin bir MİK değeri göstermemiştir. Sonuç olarak U. minor yapraklarının etilasetat ekstresinin H. pilori enfeksiyonlarında tedavi edici olarak kullanılabileceği düşünülmektedir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Karadağ A, Caskurlu A, Tosun F (2020). In Vitro Anti-Helicobacter pylori and Antimycobacterial Activity Evaluation of Selected Plants From Turkey. , 231 - 235. 10.35229/jaes.689341 |
| Chicago | Karadağ Ayşe Esra,Caskurlu Aysegul,Tosun Fatma In Vitro Anti-Helicobacter pylori and Antimycobacterial Activity Evaluation of Selected Plants From Turkey. (2020): 231 - 235. 10.35229/jaes.689341 |
| MLA | Karadağ Ayşe Esra,Caskurlu Aysegul,Tosun Fatma In Vitro Anti-Helicobacter pylori and Antimycobacterial Activity Evaluation of Selected Plants From Turkey. , 2020, ss.231 - 235. 10.35229/jaes.689341 |
| AMA | Karadağ A,Caskurlu A,Tosun F In Vitro Anti-Helicobacter pylori and Antimycobacterial Activity Evaluation of Selected Plants From Turkey. . 2020; 231 - 235. 10.35229/jaes.689341 |
| Vancouver | Karadağ A,Caskurlu A,Tosun F In Vitro Anti-Helicobacter pylori and Antimycobacterial Activity Evaluation of Selected Plants From Turkey. . 2020; 231 - 235. 10.35229/jaes.689341 |
| IEEE | Karadağ A,Caskurlu A,Tosun F "In Vitro Anti-Helicobacter pylori and Antimycobacterial Activity Evaluation of Selected Plants From Turkey." , ss.231 - 235, 2020. 10.35229/jaes.689341 |
| ISNAD | Karadağ, Ayşe Esra vd. "In Vitro Anti-Helicobacter pylori and Antimycobacterial Activity Evaluation of Selected Plants From Turkey". (2020), 231-235. https://doi.org/10.35229/jaes.689341 |
| APA | Karadağ A, Caskurlu A, Tosun F (2020). In Vitro Anti-Helicobacter pylori and Antimycobacterial Activity Evaluation of Selected Plants From Turkey. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES, 5(2), 231 - 235. 10.35229/jaes.689341 |
| Chicago | Karadağ Ayşe Esra,Caskurlu Aysegul,Tosun Fatma In Vitro Anti-Helicobacter pylori and Antimycobacterial Activity Evaluation of Selected Plants From Turkey. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES 5, no.2 (2020): 231 - 235. 10.35229/jaes.689341 |
| MLA | Karadağ Ayşe Esra,Caskurlu Aysegul,Tosun Fatma In Vitro Anti-Helicobacter pylori and Antimycobacterial Activity Evaluation of Selected Plants From Turkey. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES, vol.5, no.2, 2020, ss.231 - 235. 10.35229/jaes.689341 |
| AMA | Karadağ A,Caskurlu A,Tosun F In Vitro Anti-Helicobacter pylori and Antimycobacterial Activity Evaluation of Selected Plants From Turkey. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES. 2020; 5(2): 231 - 235. 10.35229/jaes.689341 |
| Vancouver | Karadağ A,Caskurlu A,Tosun F In Vitro Anti-Helicobacter pylori and Antimycobacterial Activity Evaluation of Selected Plants From Turkey. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES. 2020; 5(2): 231 - 235. 10.35229/jaes.689341 |
| IEEE | Karadağ A,Caskurlu A,Tosun F "In Vitro Anti-Helicobacter pylori and Antimycobacterial Activity Evaluation of Selected Plants From Turkey." JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES, 5, ss.231 - 235, 2020. 10.35229/jaes.689341 |
| ISNAD | Karadağ, Ayşe Esra vd. "In Vitro Anti-Helicobacter pylori and Antimycobacterial Activity Evaluation of Selected Plants From Turkey". JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES 5/2 (2020), 231-235. https://doi.org/10.35229/jaes.689341 |
