Phenotypic and molecular characterization of Salmonella Enteritidis isolates

ALTINTAS, Levent; Tutun, Hidayet; arslantas, tutku; KARAGOZ, ALPER; Kocak, Nadir; ALTINTAŞ, Özlem

doi:10.33988/auvfd. 691746

Phenotypic and molecular characterization of Salmonella Enteritidis isolates

Alper KARAGÖZ, (Uşak Üniversitesi, Moleküler Biyoloji ve Genetik Bölümü, Uşak, Türkiye)

Levent ALTINTAŞ, (Ankara Üniversitesi, Veteriner Fakültesi, Farmakoloji ve Toksikoloji Anabilim Dalı, Ankara, Türkiye)

Tutku ARSLANTAŞ, (Çankırı Devlet Hastanesi, Mikrobiyoloji Laboratuvarı, Çankırı, Türkiye)

Hidayet TUTUN, (Burdur Mehmet Akif Ersoy Üniversitesi, Veteriner Fakültesi, Farmakoloji ve Toksikoloji Anabilim Dalı, Burdur, Türkiye)

Nadir KOÇAK, (Selçuk Üniversitesi, Tıbbi Genetik, Konya, Türkiye)

Özlem ALTINTAŞ (Veteriner Kontrol Merkez Araştırma Enstitüsü, Ankara, Türkiye)

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Yıl: 2021 Cilt: 68 Sayı: 2 Sayfa Aralığı: 113 - 120 Metin Dili: İngilizce DOI: 10.33988/auvfd. 691746 İndeks Tarihi: 04-05-2021

Phenotypic and molecular characterization of Salmonella Enteritidis isolates

Öz:

Salmonella spp. is the most frequently isolated foodborne pathogens causing human and animal diseases. The aim of this study was to investigate antimicrobial susceptibility profiles and the molecular typing of 200 Salmonella Enteritidis strains isolated from the patients’ stools between 2016 and 2019 in Turkey. The isolates were examined for antibiotic susceptibility patterns (21 antimicrobial agents) by Kirby-Bauer disc diffusion method or molecular typing by Pulsed-field gel electrophoresis (PFGE) and plasmid profiling. Although all isolates were susceptible to four antibiotics (suphamethoxazole/trimethoprim, chloramphenicol,streptomycin and trimethoprim), all were resistant to 15 different antibiotics. In the PFGE study performed with XbaI enzyme, all isolates were found to be related to each other according to similarity rates of 85% and above. There were two major clones, clone A and B. Clone A was divided into 6 pulsotypes (A1-A2-A3-A4-A5-A6) and clone B was divided into 3 pulsotypes (B1-B2-B3). Clone A had 87% similarity and Clone B had 90% similarity. The clustering rate was 86% (172/200). All isolates harboured 1-4 plasmidranging in size from 2.5 to 57 kb and showed 6 plasmid profiles (P1-P6). All isolates carried the 57 kb plasmid individually or in combination with other plasmids. Most of the isolates 136 (68%) had P2 profile. Our findings indicate that the majority of all isolates were clonally related and had cross contamination problems. In this study, the importance of molecular typing methods in order to take more effective protection and control measures against Salmonella has been demonstrated and proposed to use such methods.

Anahtar Kelime:

Phenotypic and molecular characterization of Salmonella Enteritidis isolates

Öz:

Salmonella spp. insan ve hayvan hastalıklarına neden olan en yaygın gıda kaynaklı patojendir. Bu çalışmanın amacı, Türkiye'de 2016 ve 2019 yılları arasında hasta dışkılarından izole edilen 200 Salmonella Enteritidis izolatının antimikrobiyal duyarlılık profillerini ve moleküler tiplerini araştırmaktır. İzolatlar Kirby-Bauer disk difüzyon yöntemi ile antibiyotik duyarlılık profilleri (21antibiyotik) ve pulsed-field jel elektroforezi (PFGE) ve plazmid profili ile moleküler tipleme açısından incelendi. Toplam 200 izolat hem 4 farklı antimikrobiyal ajana (sülfametoksazol / trimetoprim, kloramfenikol, streptomisin ve trimetoprim) duyarlı hem de 15 farklı antimikrobiyal ajana dirençliydi. XbaI enzimi ile yapılan PFGE çalışmasında, tüm izolatların %85 ve üzeri benzerlik oranlarına göre birbirleriyle ilişkili olduğu bulundu. İki ana klon vardı, klon A ve B. Klon A, 6 pulsotipe (A1-A2-A3-A4-A5-A6) ve klon B, 3 pulsotipe(B1-B2-B3) ayrıldı. Klon A ve B, sırasıyla %87 ve %90 benzerliğe sahipti. Kümeleme oranı %86 (172/200) idi. Tüm izolatlar, 2,5 ila 57 kb arasında değişen 1-4 plazmid barındırdı ve 6 plazmid profili (P1-P6) gösterdi. Tüm izolatlar 57 kb plazmidi tek veya diğer plazmidlerle birlikte taşıdı. İzolatların çoğu (n= 136, %68) P2 profiline sahipti. Bulgularımız, 200 S. Enteritidis izolatlarının çoğunun klonal olarak ilişkili olduğunu ve çapraz kontaminasyon problemleri olduğunu göstermektedir. Bu çalışmada, Salmonellosis’e karşıdaha etkin korunma ve kontrol önlemlerinin alınmasında moleküler tipleme yöntemlerinin önemi gösterilmiş ve bu yöntemlerin kullanılması önerilmiştir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KARAGOZ A, ALTINTAS L, arslantas t, Tutun H, Kocak N, ALTINTAŞ Ö (2021). Phenotypic and molecular characterization of Salmonella Enteritidis isolates. , 113 - 120. 10.33988/auvfd. 691746
Chicago	KARAGOZ ALPER,ALTINTAS Levent,arslantas tutku,Tutun Hidayet,Kocak Nadir,ALTINTAŞ Özlem Phenotypic and molecular characterization of Salmonella Enteritidis isolates. (2021): 113 - 120. 10.33988/auvfd. 691746
MLA	KARAGOZ ALPER,ALTINTAS Levent,arslantas tutku,Tutun Hidayet,Kocak Nadir,ALTINTAŞ Özlem Phenotypic and molecular characterization of Salmonella Enteritidis isolates. , 2021, ss.113 - 120. 10.33988/auvfd. 691746
AMA	KARAGOZ A,ALTINTAS L,arslantas t,Tutun H,Kocak N,ALTINTAŞ Ö Phenotypic and molecular characterization of Salmonella Enteritidis isolates. . 2021; 113 - 120. 10.33988/auvfd. 691746
Vancouver	KARAGOZ A,ALTINTAS L,arslantas t,Tutun H,Kocak N,ALTINTAŞ Ö Phenotypic and molecular characterization of Salmonella Enteritidis isolates. . 2021; 113 - 120. 10.33988/auvfd. 691746
IEEE	KARAGOZ A,ALTINTAS L,arslantas t,Tutun H,Kocak N,ALTINTAŞ Ö "Phenotypic and molecular characterization of Salmonella Enteritidis isolates." , ss.113 - 120, 2021. 10.33988/auvfd. 691746
ISNAD	KARAGOZ, ALPER vd. "Phenotypic and molecular characterization of Salmonella Enteritidis isolates". (2021), 113-120. https://doi.org/10.33988/auvfd. 691746

APA	KARAGOZ A, ALTINTAS L, arslantas t, Tutun H, Kocak N, ALTINTAŞ Ö (2021). Phenotypic and molecular characterization of Salmonella Enteritidis isolates. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 68(2), 113 - 120. 10.33988/auvfd. 691746
Chicago	KARAGOZ ALPER,ALTINTAS Levent,arslantas tutku,Tutun Hidayet,Kocak Nadir,ALTINTAŞ Özlem Phenotypic and molecular characterization of Salmonella Enteritidis isolates. Ankara Üniversitesi Veteriner Fakültesi Dergisi 68, no.2 (2021): 113 - 120. 10.33988/auvfd. 691746
MLA	KARAGOZ ALPER,ALTINTAS Levent,arslantas tutku,Tutun Hidayet,Kocak Nadir,ALTINTAŞ Özlem Phenotypic and molecular characterization of Salmonella Enteritidis isolates. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol.68, no.2, 2021, ss.113 - 120. 10.33988/auvfd. 691746
AMA	KARAGOZ A,ALTINTAS L,arslantas t,Tutun H,Kocak N,ALTINTAŞ Ö Phenotypic and molecular characterization of Salmonella Enteritidis isolates. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2021; 68(2): 113 - 120. 10.33988/auvfd. 691746
Vancouver	KARAGOZ A,ALTINTAS L,arslantas t,Tutun H,Kocak N,ALTINTAŞ Ö Phenotypic and molecular characterization of Salmonella Enteritidis isolates. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2021; 68(2): 113 - 120. 10.33988/auvfd. 691746
IEEE	KARAGOZ A,ALTINTAS L,arslantas t,Tutun H,Kocak N,ALTINTAŞ Ö "Phenotypic and molecular characterization of Salmonella Enteritidis isolates." Ankara Üniversitesi Veteriner Fakültesi Dergisi, 68, ss.113 - 120, 2021. 10.33988/auvfd. 691746
ISNAD	KARAGOZ, ALPER vd. "Phenotypic and molecular characterization of Salmonella Enteritidis isolates". Ankara Üniversitesi Veteriner Fakültesi Dergisi 68/2 (2021), 113-120. https://doi.org/10.33988/auvfd. 691746