Yıl: 2021 Cilt: 68 Sayı: 2 Sayfa Aralığı: 121 - 127 Metin Dili: İngilizce DOI: 10.33988/auvfd.706602 İndeks Tarihi: 04-05-2021

Evaluation of the enrofloxacin excretion in Anatolian buffalo milk

Öz:
Enrofloxacin (ENR) is a broad-spectrum fluoroquinolone antibiotic widely used in both human medicine and veterinary medicine. In this study, the milk-excretion patterns of ENR and its metabolite ciprofloxacin (CIP) were determined up to the 10th milking following the intramuscular administration of ENR (2.5mg/kg of body weight) to lactating dairy buffaloes. The milk of each animal was collected and the concentrations of ENR and CIP were detected in milk by LC-MS/MS. The detection limits of the method were determined for ENR and CIP as 0.35 μg/kg and 0.12 μg/kg, respectively. The highest total mean concentrations of ENR and its metabolite CIP was determined in the second milking after injection as 603 μg/kg. The residue level in all buffalo milk samples was found to be lower than the maximum residue limit (100 μg/kg) at the fifth milking. In addition, the employed method is successfully applied to evaluate the presence of ENR and CIP residue in 50 marketed buffalo milk samples and none of the samples contained these antibiotics. Consequently, the present study provided information on the milk excretion levels of ENR and CIP in Anatolian buffalo milks by an LC-MS/MS method.
Anahtar Kelime:

Anadolu manda sütündeki enrofloksasin atılımının değerlendirilmesi

Öz:
Enrofloksasin (ENR), hem beşeri hekimlikte hem de veteriner hekimlikte yaygın olarak kullanılan geniş spektrumlu bir florokinolon antibiyotiktir. Bu çalışmada laktasyondaki mandalara ENR'nin (2,5 mg/kg vücut ağırlığı) intramusküler uygulamasını takiben 10. sağımına kadar ENR ve metaboliti siprofloksasinin (CIP) süt ile atılımı belirlendi. Her bir hayvanın sütü toplanarak ENR ve CIP düzeyleri LC-MS/MS ile tespit edildi. Yöntemin tespit sınırları ENR ve CIP için sırasıyla 0,35 μg/kg ve 0,12 μg/kg olarak saptandı. ENR ve metaboliti CIP'nin en yüksek toplam düzeyleri, enjeksiyondan sonraki ikinci sağımda 603 μg/kg olarak tespit edildi. Tüm manda sütü numunelerindeki kalıntı seviyesinin, beşinci sağımda maksimum kalıntı limitinden (100 μg/kg) daha düşük olduğu belirlendi. Ayrıca, kullanılan yöntem, tüketime sunulan 50 manda sütü örneğinde ENR ve CIP kalıntı varlığını değerlendirmek içinbaşarılı bir şekilde uygulandı ve hiçbir örneğinin bu antibiyotikleri içermediği saptandı. Sonuç olarak bu çalışma, LC-MS/MS yöntemi kullanılarak Anadolu mandalarında ENR ve CIP'in süt atılım seviyeleri hakkında bilgi sağladı. Anahtar sözcükler: Anadolu mandası, atılım, enrofloksasin, LC-MS/MS, siprofloksasin, süt.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Acaroz U, Ince S, ARSLAN ACAROZ D, GÜRLER Z, KARA R, KÜÇÜKKURT I, ERYAVUZ A (2021). Evaluation of the enrofloxacin excretion in Anatolian buffalo milk. , 121 - 127. 10.33988/auvfd.706602
Chicago Acaroz Ulas,Ince Sinan,ARSLAN ACAROZ DAMLA,GÜRLER ZEKI,KARA RECEP,KÜÇÜKKURT ISMAIL,ERYAVUZ ABDULLAH Evaluation of the enrofloxacin excretion in Anatolian buffalo milk. (2021): 121 - 127. 10.33988/auvfd.706602
MLA Acaroz Ulas,Ince Sinan,ARSLAN ACAROZ DAMLA,GÜRLER ZEKI,KARA RECEP,KÜÇÜKKURT ISMAIL,ERYAVUZ ABDULLAH Evaluation of the enrofloxacin excretion in Anatolian buffalo milk. , 2021, ss.121 - 127. 10.33988/auvfd.706602
AMA Acaroz U,Ince S,ARSLAN ACAROZ D,GÜRLER Z,KARA R,KÜÇÜKKURT I,ERYAVUZ A Evaluation of the enrofloxacin excretion in Anatolian buffalo milk. . 2021; 121 - 127. 10.33988/auvfd.706602
Vancouver Acaroz U,Ince S,ARSLAN ACAROZ D,GÜRLER Z,KARA R,KÜÇÜKKURT I,ERYAVUZ A Evaluation of the enrofloxacin excretion in Anatolian buffalo milk. . 2021; 121 - 127. 10.33988/auvfd.706602
IEEE Acaroz U,Ince S,ARSLAN ACAROZ D,GÜRLER Z,KARA R,KÜÇÜKKURT I,ERYAVUZ A "Evaluation of the enrofloxacin excretion in Anatolian buffalo milk." , ss.121 - 127, 2021. 10.33988/auvfd.706602
ISNAD Acaroz, Ulas vd. "Evaluation of the enrofloxacin excretion in Anatolian buffalo milk". (2021), 121-127. https://doi.org/10.33988/auvfd.706602
APA Acaroz U, Ince S, ARSLAN ACAROZ D, GÜRLER Z, KARA R, KÜÇÜKKURT I, ERYAVUZ A (2021). Evaluation of the enrofloxacin excretion in Anatolian buffalo milk. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 68(2), 121 - 127. 10.33988/auvfd.706602
Chicago Acaroz Ulas,Ince Sinan,ARSLAN ACAROZ DAMLA,GÜRLER ZEKI,KARA RECEP,KÜÇÜKKURT ISMAIL,ERYAVUZ ABDULLAH Evaluation of the enrofloxacin excretion in Anatolian buffalo milk. Ankara Üniversitesi Veteriner Fakültesi Dergisi 68, no.2 (2021): 121 - 127. 10.33988/auvfd.706602
MLA Acaroz Ulas,Ince Sinan,ARSLAN ACAROZ DAMLA,GÜRLER ZEKI,KARA RECEP,KÜÇÜKKURT ISMAIL,ERYAVUZ ABDULLAH Evaluation of the enrofloxacin excretion in Anatolian buffalo milk. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol.68, no.2, 2021, ss.121 - 127. 10.33988/auvfd.706602
AMA Acaroz U,Ince S,ARSLAN ACAROZ D,GÜRLER Z,KARA R,KÜÇÜKKURT I,ERYAVUZ A Evaluation of the enrofloxacin excretion in Anatolian buffalo milk. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2021; 68(2): 121 - 127. 10.33988/auvfd.706602
Vancouver Acaroz U,Ince S,ARSLAN ACAROZ D,GÜRLER Z,KARA R,KÜÇÜKKURT I,ERYAVUZ A Evaluation of the enrofloxacin excretion in Anatolian buffalo milk. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2021; 68(2): 121 - 127. 10.33988/auvfd.706602
IEEE Acaroz U,Ince S,ARSLAN ACAROZ D,GÜRLER Z,KARA R,KÜÇÜKKURT I,ERYAVUZ A "Evaluation of the enrofloxacin excretion in Anatolian buffalo milk." Ankara Üniversitesi Veteriner Fakültesi Dergisi, 68, ss.121 - 127, 2021. 10.33988/auvfd.706602
ISNAD Acaroz, Ulas vd. "Evaluation of the enrofloxacin excretion in Anatolian buffalo milk". Ankara Üniversitesi Veteriner Fakültesi Dergisi 68/2 (2021), 121-127. https://doi.org/10.33988/auvfd.706602