Prevalence and molecular diagnosis of Gongylonema pulchrumin cattle and sheep in the Samsun region
Yıl: 2021 Cilt: 68 Sayı: 2 Sayfa Aralığı: 129 - 135 Metin Dili: İngilizce DOI: 10.33988/auvfd.710010 İndeks Tarihi: 04-05-2021
Prevalence and molecular diagnosis of Gongylonema pulchrumin cattle and sheep in the Samsun region
Öz: This study was carried out between November-2017 and June-2019 to determine the distribution and molecular characterization of Gongylonema pulchrumin cattle and sheep slaughtered in local abattoirs and slaughtering areas during the feast of sacrifice. In this study, esophagi collected from 380 (358 young and 22 mature) cattle and 848 esophagi from sheep (816 young and 32 old) were examined. Esophagus taken after slaughtering was brought to the laboratory in plastic bag. In the laboratory, all the esophagi were cut open with scissors and all of the materials were examined using a loupe under a light source. Parasites were taken out using eye forceps and placed in 70% ethyl alcohol. Following the primary morphological examination, all the parasites cut into three parts, anterior and posterior parts were used for morphologic identification and mid part was used for molecular identification. BpCoxI-F1 and BpCoxI-R1 primers were used for molecular identification.Only two cows (older than four years) were infected and the infection rate was 0.53%. A total of 27 parasites were identified, 18 were male and 9 were female. Parasites were not found in the examined sheep. All the samples collected from the infected cattle were identified as G. pulchrumafter morphological examinations and molecular confirmation and positive bands were detected at 400bp
Anahtar Kelime: Samsun yöresinde sığır ve koyunlarda Gongylonema pulchrum’un yaygınlığı ve moleküler teşhisi
Öz: Bu araştırma, Samsun bölgesinde 2017 Kasım -2019 Haziran tarihleri arasında, yerel mezbahalar ve kurban bayramında kesilen koyun ve sığırlarda Gongylonema pulchrum’un yaygınlığı ve moleküler karakterizasyonunu belirlemek amacıyla yapıldı. Çalışmada 380 sığır (22’si yaşlı, 358’i genç) ve 848 koyundan (32’si yaşlı, 816’sı genç)toplanan özefaguslar incelendi. Kesim sonrası alınan özofaguslar plastik torbalara konularak laboratuvara getirildi. Laboratuvarda tüm özefaguslar makas yardımı ile açılarak ışık altında ve büyüteç yardımı ile incelendi. Parazitler göz pensi ile çıkartılıp % 70’lik etil alkole konuldu. Parazitler üç parçaya ayrıldı, ön ve arka kısımlar morfolojik, orta kısımları ise moleküler teşhis amacıyla kullanıldı. Moleküler teşhis için Bp CoxI-F1 ve Bp CoxI-R1 primerleri kullanıldı. Yalnızca iki inek (dört yaş üstü) enfekte olmuş ve yaygınlık oranı % 0,53 bulunmuştur.Enfekte hayvanlardan 18 erkek ve 9 dişi olmak üzere 27 parazit toplandı. İncelenen koyunlarda parazite rastlanmadı. Enfekte sığırdan toplanan tüm örnekler, morfolojik incelemeler ve moleküler doğrulama sonrasında G.pulchrumolarak saptandı ve pozitif bantlar 400 bp’de elde edildi.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Gürel T, Umur Ş (2021). Prevalence and molecular diagnosis of Gongylonema pulchrumin cattle and sheep in the Samsun region. , 129 - 135. 10.33988/auvfd.710010 |
| Chicago | Gürel Taner,Umur Şinasi Prevalence and molecular diagnosis of Gongylonema pulchrumin cattle and sheep in the Samsun region. (2021): 129 - 135. 10.33988/auvfd.710010 |
| MLA | Gürel Taner,Umur Şinasi Prevalence and molecular diagnosis of Gongylonema pulchrumin cattle and sheep in the Samsun region. , 2021, ss.129 - 135. 10.33988/auvfd.710010 |
| AMA | Gürel T,Umur Ş Prevalence and molecular diagnosis of Gongylonema pulchrumin cattle and sheep in the Samsun region. . 2021; 129 - 135. 10.33988/auvfd.710010 |
| Vancouver | Gürel T,Umur Ş Prevalence and molecular diagnosis of Gongylonema pulchrumin cattle and sheep in the Samsun region. . 2021; 129 - 135. 10.33988/auvfd.710010 |
| IEEE | Gürel T,Umur Ş "Prevalence and molecular diagnosis of Gongylonema pulchrumin cattle and sheep in the Samsun region." , ss.129 - 135, 2021. 10.33988/auvfd.710010 |
| ISNAD | Gürel, Taner - Umur, Şinasi. "Prevalence and molecular diagnosis of Gongylonema pulchrumin cattle and sheep in the Samsun region". (2021), 129-135. https://doi.org/10.33988/auvfd.710010 |
| APA | Gürel T, Umur Ş (2021). Prevalence and molecular diagnosis of Gongylonema pulchrumin cattle and sheep in the Samsun region. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 68(2), 129 - 135. 10.33988/auvfd.710010 |
| Chicago | Gürel Taner,Umur Şinasi Prevalence and molecular diagnosis of Gongylonema pulchrumin cattle and sheep in the Samsun region. Ankara Üniversitesi Veteriner Fakültesi Dergisi 68, no.2 (2021): 129 - 135. 10.33988/auvfd.710010 |
| MLA | Gürel Taner,Umur Şinasi Prevalence and molecular diagnosis of Gongylonema pulchrumin cattle and sheep in the Samsun region. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol.68, no.2, 2021, ss.129 - 135. 10.33988/auvfd.710010 |
| AMA | Gürel T,Umur Ş Prevalence and molecular diagnosis of Gongylonema pulchrumin cattle and sheep in the Samsun region. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2021; 68(2): 129 - 135. 10.33988/auvfd.710010 |
| Vancouver | Gürel T,Umur Ş Prevalence and molecular diagnosis of Gongylonema pulchrumin cattle and sheep in the Samsun region. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2021; 68(2): 129 - 135. 10.33988/auvfd.710010 |
| IEEE | Gürel T,Umur Ş "Prevalence and molecular diagnosis of Gongylonema pulchrumin cattle and sheep in the Samsun region." Ankara Üniversitesi Veteriner Fakültesi Dergisi, 68, ss.129 - 135, 2021. 10.33988/auvfd.710010 |
| ISNAD | Gürel, Taner - Umur, Şinasi. "Prevalence and molecular diagnosis of Gongylonema pulchrumin cattle and sheep in the Samsun region". Ankara Üniversitesi Veteriner Fakültesi Dergisi 68/2 (2021), 129-135. https://doi.org/10.33988/auvfd.710010 |
