The effects of different storage conditions and periods on mould-yeast,aflatoxin, E. coli and E. coli O157 in wet sugar beet pulp

CEYLANER, ahmet; SUR, ASLIHAN; birben, nurgül; TATLI SEVEN, PINAR; KARAKUŞ, Kerem; SEVEN, ISMAIL

doi:10.33988/auvfd.753418

The effects of different storage conditions and periods on mould-yeast,aflatoxin, E. coli and E. coli O157 in wet sugar beet pulp

Pınar TATLI SEVEN, (Fırat Üniversitesi, Veteriner Fakültesi, Hayvan Besleme ve Beslenme Hastalıkları Anabilim Dalı, Elazığ, Türkiye)

İsmail SEVEN, (Fırat Üniversitesi, Sivrice Meslek Yüksekokulu, Bitkisel ve Hayvansal Üretim Bölümü, Elazığ, Türkiye)

Nurgül BİRBEN, (Veteriner Kontrol Enstitüsü, Elazığ, Türkiye)

Kerem KARAKUŞ, (Veteriner Kontrol Enstitüsü, Elazığ, Türkiye)

Aslıhan Sur ARSLAN, (Balıkesir Üniversitesi, Kepsut Meslek Yüksekokulu, Veterinerlik Anabilim Dalı, Balıkesir, Türkiye)

Ahmet CEYLANER (Fırat Üniversitesi, Veteriner Fakültesi, Hayvan Besleme ve Beslenme Hastalıkları Anabilim Dalı, Elazığ, Türkiye)

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Yıl: 2021 Cilt: 68 Sayı: 2 Sayfa Aralığı: 161 - 166 Metin Dili: İngilizce DOI: 10.33988/auvfd.753418 İndeks Tarihi: 04-05-2021

The effects of different storage conditions and periods on mould-yeast,aflatoxin, E. coli and E. coli O157 in wet sugar beet pulp

Öz:

This study was designed to determine the effects of storage condition and period on mold-yeast, aflatoxin, E. coliand E. coli O157 in the wet sugar beet pulp (WSBP). For this aim, samples were taken from the first production of WSBP from the sugar factory (Control) and the newly packaged sacks from the packaging company (Packaged Control). Afterwards, additional two samples were taken from WSBP stored as packaged (Packaged) and as surrounded by a nylon cover outside near the barn (Nonpackaged) from the predetermined farms with one-month interval. The pH value of the Packaged group was significantly lower than the Non-packaged group at the end of the 1st and 2nd months (P<0.01). The yeast-mold count of Non-packaged and Packaged groups at the end of the 1st and 2nd months was determined to be 4.61, 1.60 and 4.83, 1.26 log10 CFU/g, respectively (P<0.01). However, aflatoxin (B1, B2, G1 and G2) was not detected in groups. The E. coli was detected as 1.48 (in the 1st month) and 1.53 (in the 2ndmonth) log10 CFU/g in only Non-packaged group. It was worked for the identification of E. coli O157 from E. coli positive samples of Non-packaged group, but it was not detected. Consequently, it was determined that the use of packaged WSBP was better than the Non-packaged in terms of microbiological analysis.

Anahtar Kelime:

Yaş şeker pancarı posasında farklı depolama koşulu ve periyodunun küf-maya, aflatoksin, E. coli ve E. coli O157 üzerine etkileri

Öz:

Bu araştırma, yaş şeker pancarı posasında (WSBP) küf-maya, aflatoksin, E. coli ve E. coli O157 üzerine depolama koşulu ve süresinin etkilerini belirlemek amacıyla planlanmıştır. Bu amaçla, fabrikadan WSBP’nın ilk üretimi (Kontrol) ve paketleme işletmesinin yeni paketlediği çuvallardan (Paket Kontrol) örnekler alınmıştır. Daha sonra, birer ay aralıklarla önceden belirlenmişçiftliklerden paketlenmiş (Paketlenmiş) ve ahırın yakınında dışarıda bir naylonla çevrili olarak saklanan (Açık) WSBP’ndan iki numune daha alınmıştır. Paketlenmiş grubun pH değeri, 1. ve 2. ayların sonunda açık grubunkilerden önemli derecede daha düşüktü (P<0,01). 1. ve 2. ayların sonunda açık ve paketlenmiş grupların maya-küf sayısı sırasıyla, 4,61; 1,60 ve 4,83; 1,26 log10 CFU/g olarak belirlenmiştir (P<0,01). Bununla birlikte, gruplarda aflatoksin (B1, B2, G1, G2) tespit edilmemiştir. E. coli sadece açık grupta, 1,48(birinci ayda) ve 1,53 (ikinci ayda) log10 CFU/g olarak belirlenmiştir. Açık grubun pozitif E. coli örneklerinde E. coli O157identifikasyonuna çalışılmış, ancak tespit edilmemiştir. Sonuç olarak, mikrobiyolojik analizler açısından paketlenmiş WSBP kullanımının paketlenmemişten daha iyi olduğu belirlenmiştir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Akkaya MR, Bal MA, Duman AD (2005): Yemlerde ve Yem Hammaddelerinde Görülen Mikotoksinlerin Hayvan Sağlığı Üzerine Etkileri ve Analiz Yöntemleri. 459-462. III. Ulusal Hayvan Besleme Kongresi, Adana, Türkiye.
2. Altaçlı S, Deniz S (2013): The tetermination of in vivo and in vitro digestibility and energy contents of sugar beet pulp silages produced in different ways. Van Vet J, 24, 9-13.
3. Anonymous (2008): ISO 21527-1. Microbiology of food and animal feeding stuffs. Horizontal method for the enumeration of yeasts and moulds (first edition; 2008.07.01). Available at: https://www.sis.se/api/ document/preview/910016/(Accessed June 03, 2020).
4. Anonymous (2020): Regulation on sampling and analysis methods for official control of feeds. Available at: https://www.resmigazete.gov.tr/eskiler/2011/12/20111227- 13.htm(Accessed June 03, 2020).
5. AOAC (2003): International, AOAC Official Method 2003.02.
6. Baran MS, Erkan ME, Vural A (2008): The nutrient values and microbiological quality properties of mixed feeds used in ruminant nutrition in Diyarbakir province. J Fac Vet Med İstanbul Univ, 34, 9-19.
7. Bearson S, Bearson B, Foster JW (1997): Acid stress responses in enterobacteria. FEMS Microbiol Lett, 147, 173-180.
8. Bell C (2002): Approach to the control of Enterohaemorrhagic Escherichia coli (EHEC). Int J Food Microbiol, 78, 197- 216.
9. Buchanan RL, Edelson RL (1999): pH-dependent stationary- phase acid resistance response of enterohaemorrhagic Escherichia coli in the presence of various acidulants. J Food Prot, 62, 211-218.
10. Çerçi İH, Çiftçi M, Güler T, et al (2005): Silolamada Yoncaya Şeker ve Arpaya Alternatif Olarak Pazarlanamayan Elmanın Katılma Olanağının Araştırılması. III. Ulusal Hayvan Besleme Kongresi. Adana, Türkiye.
11. Ceylaner A, Tatli Seven P, Seven I, et al (2020): The effects of two different storage condition and duration on nutrient level and in vitro digestibility of wet sugar beet pulp. FÜ Sağ Bil Vet Derg, 34, 7-12.
12. Coia JE (1998): Clinical, Microbiological and epidemiological aspects of Escherichia coli O157: H7 Infections. FEMS Immunol Med Microbiol, 20, 1-9.
13. Erdoğan Z, Aslantaş Ö (2004): A survey on the microbiological quality of mixed feeds and feedstuffs used in Hatay province. Vet Bil Derg, 20, 33-38.
14. Ergün A, Tuncer ŞD, Çolpan İ, et al (2016): Yem Hijyeni ve Teknolojisi. Ankara Üniversitesi Veteriner Fakültesi Ders Kitabı, Ankara, Türkiye.
15. FAO (2013): Quality assurance for animal feed analysis laboratories. FAO Animal Production and Health Manual No. 16. Rome, Italy.
16. Feng P, Weagant SD, Jinneman K, et al (2018): Diarrheagenic Escherichia Coli. In: Bacteriological Analytical Manual.
17. Gürsoy A (2018): Nutrition and Development of Microorganisms. Available at: http://cv.ankara.edu.tr/ duzenleme/kisisel/dosyalar/03112017135411.pdf (Accessed July 8, 2020).
18. Halkman AK, Noveir MR, Doğan HB (2001): Escherichia coli O157:H7 Serotipi. Sim Matbaacılık Ltd. Şti, Ankara, Türkiye.
19. Huijbregts T, Legrand G, Hoffmann C, et al (2013): Long-term storage of sugar beet in North-West Europe. COBRI, Report no. 1-2013.
20. IBM SPSS (2013): IBM SPSS Statistics for Windows, Version 22.0. Armonk, NY, USA.
21. İptaş S, Yılmaz M, Öz A, et al (1997): Tokat Ekolojik Şartlarında Silajlık Mısır, Sorgum Tür Ve Melezlerinden Yararlanma Olanakları. 287. Türkiye Birinci Silaj Kongresi. Hasad Yayıncılık, İstanbul, Türkiye.
22. Karakaya Y, Atasever M (2010): Aflatoxin B1 in corn silage and its probability passing in milk. Kafkas Univ Vet Fak Derg, 16, 123-127.
23. Kaya Ş (2017): Practical guide for some aerobic spoilage in silage. MKÜ Ziraat Fak Derg, 22, 127-134.
24. Kubadinov N, Hollaus F, Braunsteiner W (1984): Beiträge zur Aufklärung mikrobiologischer und chemischer Zusammenhänge bei der Pressschnitzelsilierung. 3. Mitteilung. Veränderung der chemischen Zusammensetzung von Pressschnitzeln während des Silierprozesses. Zuckerindustrie, 109, 38-45.
25. Kumar P, Mahato DK, Kamle M, et al (2017): Aflatoxins: A global concern for food safety, human health and their management. Front Microbiol, 7, 2170.
26. Lin J, Smith MP, Chopin KC, et al (1996): Mechanisms of acid resistance in enterohaemorrhagic Escherichia coli. Appl Environ Microbiol, 62, 3094-3100.
27. Öksüztepe G, Erkan S (2016): Mycotoxins and their importance in terms of public health. Harran Univ Vet Fak Derg, 5, 190-195.
28. Sweeney MJ, Dobson ADW (1998): Mycotoxin production by Aspergillus, Fusarium and Penicillium species. Int J of Food Mic, 43, 141-158.
29. Şahin K, Sarı M (1996): Elazığ yöresinde yaygın olarak kullanılan yemlerin bakteri ve mantar florası üzerine bir araştırma. F Ü Sağ Bil Derg, 10, 251-258.
30. Tosun H, Gönül ŞA (2003): E. coli O157:H7’nin aside tolerans kazanması ve asidik gıdalarda önemi. Orlab OnLine Mikrobiyoloji Derg, 1, 10-17.
31. Ülger İ, Kaliber M, Büyükkılıç Beyzi S, et al (2015): Yaş şeker pancarı posasının bazı meyve posaları ile silolanmasının silaj kalite özellikleri, enerji değerleri ve organik madde sindirilebilirlikleri üzerine etkisi. Alınteri, 29, 18-25.

APA	TATLI SEVEN P, SEVEN I, birben n, KARAKUŞ K, SUR A, CEYLANER a (2021). The effects of different storage conditions and periods on mould-yeast,aflatoxin, E. coli and E. coli O157 in wet sugar beet pulp. , 161 - 166. 10.33988/auvfd.753418
Chicago	TATLI SEVEN PINAR,SEVEN ISMAIL,birben nurgül,KARAKUŞ Kerem,SUR ASLIHAN,CEYLANER ahmet The effects of different storage conditions and periods on mould-yeast,aflatoxin, E. coli and E. coli O157 in wet sugar beet pulp. (2021): 161 - 166. 10.33988/auvfd.753418
MLA	TATLI SEVEN PINAR,SEVEN ISMAIL,birben nurgül,KARAKUŞ Kerem,SUR ASLIHAN,CEYLANER ahmet The effects of different storage conditions and periods on mould-yeast,aflatoxin, E. coli and E. coli O157 in wet sugar beet pulp. , 2021, ss.161 - 166. 10.33988/auvfd.753418
AMA	TATLI SEVEN P,SEVEN I,birben n,KARAKUŞ K,SUR A,CEYLANER a The effects of different storage conditions and periods on mould-yeast,aflatoxin, E. coli and E. coli O157 in wet sugar beet pulp. . 2021; 161 - 166. 10.33988/auvfd.753418
Vancouver	TATLI SEVEN P,SEVEN I,birben n,KARAKUŞ K,SUR A,CEYLANER a The effects of different storage conditions and periods on mould-yeast,aflatoxin, E. coli and E. coli O157 in wet sugar beet pulp. . 2021; 161 - 166. 10.33988/auvfd.753418
IEEE	TATLI SEVEN P,SEVEN I,birben n,KARAKUŞ K,SUR A,CEYLANER a "The effects of different storage conditions and periods on mould-yeast,aflatoxin, E. coli and E. coli O157 in wet sugar beet pulp." , ss.161 - 166, 2021. 10.33988/auvfd.753418
ISNAD	TATLI SEVEN, PINAR vd. "The effects of different storage conditions and periods on mould-yeast,aflatoxin, E. coli and E. coli O157 in wet sugar beet pulp". (2021), 161-166. https://doi.org/10.33988/auvfd.753418

APA	TATLI SEVEN P, SEVEN I, birben n, KARAKUŞ K, SUR A, CEYLANER a (2021). The effects of different storage conditions and periods on mould-yeast,aflatoxin, E. coli and E. coli O157 in wet sugar beet pulp. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 68(2), 161 - 166. 10.33988/auvfd.753418
Chicago	TATLI SEVEN PINAR,SEVEN ISMAIL,birben nurgül,KARAKUŞ Kerem,SUR ASLIHAN,CEYLANER ahmet The effects of different storage conditions and periods on mould-yeast,aflatoxin, E. coli and E. coli O157 in wet sugar beet pulp. Ankara Üniversitesi Veteriner Fakültesi Dergisi 68, no.2 (2021): 161 - 166. 10.33988/auvfd.753418
MLA	TATLI SEVEN PINAR,SEVEN ISMAIL,birben nurgül,KARAKUŞ Kerem,SUR ASLIHAN,CEYLANER ahmet The effects of different storage conditions and periods on mould-yeast,aflatoxin, E. coli and E. coli O157 in wet sugar beet pulp. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol.68, no.2, 2021, ss.161 - 166. 10.33988/auvfd.753418
AMA	TATLI SEVEN P,SEVEN I,birben n,KARAKUŞ K,SUR A,CEYLANER a The effects of different storage conditions and periods on mould-yeast,aflatoxin, E. coli and E. coli O157 in wet sugar beet pulp. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2021; 68(2): 161 - 166. 10.33988/auvfd.753418
Vancouver	TATLI SEVEN P,SEVEN I,birben n,KARAKUŞ K,SUR A,CEYLANER a The effects of different storage conditions and periods on mould-yeast,aflatoxin, E. coli and E. coli O157 in wet sugar beet pulp. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2021; 68(2): 161 - 166. 10.33988/auvfd.753418
IEEE	TATLI SEVEN P,SEVEN I,birben n,KARAKUŞ K,SUR A,CEYLANER a "The effects of different storage conditions and periods on mould-yeast,aflatoxin, E. coli and E. coli O157 in wet sugar beet pulp." Ankara Üniversitesi Veteriner Fakültesi Dergisi, 68, ss.161 - 166, 2021. 10.33988/auvfd.753418
ISNAD	TATLI SEVEN, PINAR vd. "The effects of different storage conditions and periods on mould-yeast,aflatoxin, E. coli and E. coli O157 in wet sugar beet pulp". Ankara Üniversitesi Veteriner Fakültesi Dergisi 68/2 (2021), 161-166. https://doi.org/10.33988/auvfd.753418