Comparison of Effects of Three Distinct Stress Models on Anxiety- and Depression-Related Behaviors in Female Rats

KOCA, Raviye Ozen; CAKAN, Pinar; SAHİN, Zafer; OZKURKCULER, Alpaslan; KOC, Aynur; Kutlu, Selim; GORMUS, Z. Isik Solak

Comparison of Effects of Three Distinct Stress Models on Anxiety- and Depression-Related Behaviors in Female Rats

Zafer Sahin, (Karadeniz Teknik Üniversitesi, Tıp Fakültesi, Fizyoloji Anabilim Dalı, Trabzon, Türkiye)

Aynur Koc, (Hitit Üniversitesi, Tıp Fakültesi, Fizyoloji Anabilim Dalı, Çorum, Türkiye)

Raviye Ozen Koca, (Necmettin Erbakan Üniversitesi, Meram Tıp Fakültesi, Fizyoloji Anabilim Dalı, Konya, Türkiye)

Alpaslan Ozkurkculer, (Necmettin Erbakan Üniversitesi, Meram Tıp Fakültesi, Fizyoloji Anabilim Dalı, Konya, Türkiye)

Pinar Cakan, (İnönü Üniversitesi, Tıp Fakültesi, Fizyoloji Anabilim Dalı, Malatya, Türkiye)

Z. Isik Solak Gormus, (Necmettin Erbakan Üniversitesi, Meram Tıp Fakültesi, Fizyoloji Anabilim Dalı, Konya, Türkiye)

Selim Kutlu (Necmettin Erbakan Üniversitesi, Meram Tıp Fakültesi, Fizyoloji Anabilim Dalı, Konya, Türkiye)

Sakarya Tıp Dergisi

7 0

Yıl: 2019 Cilt: 9 Sayı: 1 Sayfa Aralığı: 131 - 140 Metin Dili: İngilizce İndeks Tarihi: 22-04-2021

Comparison of Effects of Three Distinct Stress Models on Anxiety- and Depression-Related Behaviors in Female Rats

Öz:

Objective The aim of the present study was to compare the effects of three distinct stress protocols on anxiety/depression-related behaviors in adult female rats.( Sakarya Med J 2019, 9(1):131-140 )Materialsand MethodsAdult Wistar rats were randomly divided into four groups (n=8/ group) as control, immobilization stress-1 (daily 45 minutes) immobilization stress-2 (daily twice 45minutes) and social isolation (rats were housed in a metabolic cage). Stress protocols were performed for a period of 10 days. When the animals were in diestrus, anxiety anddepression-like behaviors were evaluated by the open field test and forced swimming test, respectively. Anxiety and depression tests were repeated after a 10-day rest period.Results In the open field test, a percentage of time spent in the central area was lower in the immobilization stress-2 and social isolation (p<0.05) groups and total distance was lowerin the immobilization stress-1 (p<0.01) and the social isolation groups (p<0.05). Rearing score was lower in the social isolation group (p<0.05). Swimming behaviors werelower (p<0.01), and immobility durations were higher (p<0.05) in the immobilization stress-1 and social isolation groups. In the second tests, time spent in the central areawas lower in the immobilization stress-1 (p<0.05) and the immobilization stress-2 (p<0.01) groups. Swimming behaviors were lower and immobility behaviors were higherin the immobilization stress-1 group (p<0.001) and the social isolation group (p<0.01 and p<0.001, respectively).Conclusion We suggest that depression-like behaviors are more dominant in the immobilization stress-1 and social isolation groups of adult female rats because the depression-relatedresults of these two groups are valid both after the stress period and after the rest period.

Anahtar Kelime:

Dişi Sıçanlarda Üç Farklı Stres Modelinin Anksiyete ve Depresyon Benzeri Davranışlara Etkilerinin Karşılaştırılması

Öz:

Amaç Mevcut çalışmada yetişkin dişi sıçanlarda üç farklı stres protokolünün anksiyete/depresyon benzeri davranışlara etkisinin kıyaslanması amaçlanmıştır. ( Sakarya Tıp Dergisi 2019, 9(1):131-140 ). Gereç ve Yöntemler Yetişkin Wistar sıçanlar kontrol, immobilizasyon stresi-1 (günlük 45 dakika) immobilizasyon stresi-2 (günlük iki kez 45 dakika) ve sosyal izolasyon (metabolik kafeste) olarak rastgele dört gruba ayrıldı (n=8/grup). Stres protokolleri 10 gün boyunca uygulandı ve anksiyete ve depresyonla ilişkili davranışlar, hayvanlar diöstrüs dönemindeyken, açık alan ve zorunlu yüzme testi ile değerlendirildi. Anksiyete ve depresyon testleri 10 günlük dinlenme süresinden sonra tekrarlandı. Bulgular Açık alan testinde, merkez alanda geçirilen zaman immobilizasyon stresi-2 ve sosyal izolasyon gruplarında azaldı (p<0.05). Toplam kat edilen mesafe immobilizasyon stresi-1 (p<0.01) ve sosyal izolasyon (p<0.05) gruplarında daha düşüktü. Sosyal izolasyon grubunun şahlanma davranışı (p < 0.05) kontrol grubuna göre düşüktü. İmmobilizasyon stresi-1 ve sosyal izolasyon gruplarında yüzme davranışı azalırken (p < 0.01), immobilizasyon davranışı kontrol grubuna göre artış göstermişti (p <0.05). İkinci testlerde, merkezi alanda geçirilen süre immobilizasyon stresi-1 ve immobilizasyon stresi-2 gruplarında daha düşüktü (p <0.05 ve p <0.01). İmmobilizasyon stresi-1 (p <0.001) ve sosyal izolasyon gruplarında (p <0.01) yüzme davranışı azalırken, immobilizasyon süresi kontrole kıyasla artmıştı (p <0.001). Sonuç İmmobilizasyon stresi-1 ve metabolik kafeste sosyal izolasyon protokolü uygulanan erişkin dişi sıçanlarda depresyon benzeri davranışların daha baskın olduğu söylenebilir çünkü her iki grupta da depresyonla ilişkili bulgular hem stres sonrası hem de dinlenme periyodu sonrası geçerlidir

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	SAHİN Z, KOC A, KOCA R, OZKURKCULER A, CAKAN P, GORMUS Z, Kutlu S (2019). Comparison of Effects of Three Distinct Stress Models on Anxiety- and Depression-Related Behaviors in Female Rats. , 131 - 140.
Chicago	SAHİN Zafer,KOC Aynur,KOCA Raviye Ozen,OZKURKCULER Alpaslan,CAKAN Pinar,GORMUS Z. Isik Solak,Kutlu Selim Comparison of Effects of Three Distinct Stress Models on Anxiety- and Depression-Related Behaviors in Female Rats. (2019): 131 - 140.
MLA	SAHİN Zafer,KOC Aynur,KOCA Raviye Ozen,OZKURKCULER Alpaslan,CAKAN Pinar,GORMUS Z. Isik Solak,Kutlu Selim Comparison of Effects of Three Distinct Stress Models on Anxiety- and Depression-Related Behaviors in Female Rats. , 2019, ss.131 - 140.
AMA	SAHİN Z,KOC A,KOCA R,OZKURKCULER A,CAKAN P,GORMUS Z,Kutlu S Comparison of Effects of Three Distinct Stress Models on Anxiety- and Depression-Related Behaviors in Female Rats. . 2019; 131 - 140.
Vancouver	SAHİN Z,KOC A,KOCA R,OZKURKCULER A,CAKAN P,GORMUS Z,Kutlu S Comparison of Effects of Three Distinct Stress Models on Anxiety- and Depression-Related Behaviors in Female Rats. . 2019; 131 - 140.
IEEE	SAHİN Z,KOC A,KOCA R,OZKURKCULER A,CAKAN P,GORMUS Z,Kutlu S "Comparison of Effects of Three Distinct Stress Models on Anxiety- and Depression-Related Behaviors in Female Rats." , ss.131 - 140, 2019.
ISNAD	SAHİN, Zafer vd. "Comparison of Effects of Three Distinct Stress Models on Anxiety- and Depression-Related Behaviors in Female Rats". (2019), 131-140.

APA	SAHİN Z, KOC A, KOCA R, OZKURKCULER A, CAKAN P, GORMUS Z, Kutlu S (2019). Comparison of Effects of Three Distinct Stress Models on Anxiety- and Depression-Related Behaviors in Female Rats. Sakarya Tıp Dergisi, 9(1), 131 - 140.
Chicago	SAHİN Zafer,KOC Aynur,KOCA Raviye Ozen,OZKURKCULER Alpaslan,CAKAN Pinar,GORMUS Z. Isik Solak,Kutlu Selim Comparison of Effects of Three Distinct Stress Models on Anxiety- and Depression-Related Behaviors in Female Rats. Sakarya Tıp Dergisi 9, no.1 (2019): 131 - 140.
MLA	SAHİN Zafer,KOC Aynur,KOCA Raviye Ozen,OZKURKCULER Alpaslan,CAKAN Pinar,GORMUS Z. Isik Solak,Kutlu Selim Comparison of Effects of Three Distinct Stress Models on Anxiety- and Depression-Related Behaviors in Female Rats. Sakarya Tıp Dergisi, vol.9, no.1, 2019, ss.131 - 140.
AMA	SAHİN Z,KOC A,KOCA R,OZKURKCULER A,CAKAN P,GORMUS Z,Kutlu S Comparison of Effects of Three Distinct Stress Models on Anxiety- and Depression-Related Behaviors in Female Rats. Sakarya Tıp Dergisi. 2019; 9(1): 131 - 140.
Vancouver	SAHİN Z,KOC A,KOCA R,OZKURKCULER A,CAKAN P,GORMUS Z,Kutlu S Comparison of Effects of Three Distinct Stress Models on Anxiety- and Depression-Related Behaviors in Female Rats. Sakarya Tıp Dergisi. 2019; 9(1): 131 - 140.
IEEE	SAHİN Z,KOC A,KOCA R,OZKURKCULER A,CAKAN P,GORMUS Z,Kutlu S "Comparison of Effects of Three Distinct Stress Models on Anxiety- and Depression-Related Behaviors in Female Rats." Sakarya Tıp Dergisi, 9, ss.131 - 140, 2019.
ISNAD	SAHİN, Zafer vd. "Comparison of Effects of Three Distinct Stress Models on Anxiety- and Depression-Related Behaviors in Female Rats". Sakarya Tıp Dergisi 9/1 (2019), 131-140.