Anisakis spp. infection in Atlantic mackerel (Scomber scombrus, Linnaeus 1758) from the Sea of Marmara

Gonenc, Bahadir; özbakış, gökben; aştı, ceren

doi:10.33188/vetheder.599455

Anisakis spp. infection in Atlantic mackerel (Scomber scombrus, Linnaeus 1758) from the Sea of Marmara

Gökben ÖZBAKIŞ BECERIKLISOY, (Ankara Üniversitesi, Veteriner Fakültesi, Parazitoloji Anabilim Dalı, Ankara, Türkiye)

Ceren AŞTI, (Ankara Üniversitesi, Veteriner Fakültesi, Parazitoloji Anabilim Dalı, Ankara, Türkiye)

Bahadır GÖNENÇ (Ankara Üniversitesi, Veteriner Fakültesi, Parazitoloji Anabilim Dalı, Ankara, Türkiye)

Veteriner Hekimler Derneği Dergisi

12 1

Yıl: 2020 Cilt: 91 Sayı: 1 Sayfa Aralığı: 80 - 85 Metin Dili: İngilizce DOI: 10.33188/vetheder.599455 İndeks Tarihi: 29-04-2021

Anisakis spp. infection in Atlantic mackerel (Scomber scombrus, Linnaeus 1758) from the Sea of Marmara

Öz:

This study was aimed to determine the parasite infection of fish samples. Ten Atlantic mackerel (Scomber scombrus),which were suspected with parasite infection, were sent to the laboratory by the fisherman at the Sea of Marmara, Turkey.The skin, fins, eyes, buccal cavity, and gills were examined regarding ectoparasites, while internal organs wereinvestigated concerning endoparasites. Seven of 10 fish were infected with endoparasites. A total of 27 nematode larvaewere detected from the abdominal cavity of fish. The detected nematode larvae were rinsed in 0.9 % isotonic saline andpreserved in glycerine-alcohol until examined. After that the anterior and posterior end of samples were cleared inlactophenol. The larvae had a prominent boring tooth at the anterior end. The body features of the larvae were measured(body width, oesophagus length, ventriculus length and mucron length) on a light microscope (4x-10x-40x).Morphological analysis showed that all samples were identified as Anisakis Type I larvae (L3). It is known that anisakiasisis one of the important fish-borne zoonotic diseases. Also, this nematode species had been previously reported in Turkishwater from different fish species. The present case contributes valuable information about one of the intermediate hostsof Anisakis spp. in Turkey.

Anahtar Kelime:

Marmara denizi Atlantik uskumrularındaki (Scomber scombrus, Linnaeus 1758) Anisakis spp. enfeksiyonu

Öz:

Bu çalışmada balık örneklerinde paraziter enfeksiyonların belirlenmesi amaçlandı. Marmara denizi balıkçıları tarafından paraziter enfeksiyon şüpheli on adet Atlantik uskumrusu (Scombrus scombrus) laboratuvara gönderildi. Balıklara ait deri, yüzgeçler, gözler, ağız boşluğu ve solungaçlar ektoparazitler yönünden incelenirken, iç organların da endoparazitler yönünden araştırmaları yapıldı. Gönderilen on adet balığın yedisinin enfekte olduğu saptandı. Balıkların karın boşluğunda toplam 27 nematod larvası tespit edildi. Saptanan nematod larvaları % 0.9'luk izotonik tuzlu su ile yıkandı ve gliserinalkol içinde saklandı. Sonrasında, örneklerin anterior ve posterior uçları laktofenolde şeffaflandırıldı. Larvaların ön kısmında belirgin bir kesici diş (boring tooth) görüldü. Larvaların vücut özellikleri (boy uzunluğu, özefagus uzunluğu, ventrikül ve mucron uzunluğu) ışık mikroskopu altında ölçüldü (x-4-x10-x40). Morfolojik analizler sonucu tüm örneklerin Anisakis Tip I larvaları (L3) olduğu saptandı. Anisakiasis balık-kaynaklı (fish-borne) zoonotik hastalıkların en önemlilerinden biri olarak bilinmektedir. Ayrıca, bu nematod türlerinin daha önce Türk sularında farklı balık türlerinde de bulunduğu bildirilmiştir. Bu çalışma ile, Anisakis spp.’nin Türkiye'deki ara konaklarından biri hakkında değerli bilgi elde edilecektir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Aibinu IE, Smooker PM, Lopata AL (2019): Anisakis nematodes in fish and shellfish-from infection to allergies. Int J Parasitol Parasites Wildl, 9, 384-393.
2. Akmırza A (2013): Gökçeada kıyı sularındaki balıkların parazitik nematodları. Turkiye Parazitol Derg, 37, 199-202.
3. Akmirza A (2000): Gökçeada civarında avlanan tekir (Mullus surmuletus L.) balığının metazoan parazitleri. Vet Fak Derg (İstanbul), 26(1), 129-140.
4. Akmirza A (2003): Distribution of parasites fauna of Chup mackerel in Aegean and Mediterranean Sea. Turkish J Marine Science, 9(3), 187-195.
5. Akmirza A (2012): Metazoan parasite fauna of Conger eel (Conger conger L.) near Gökçeada, northeasten Aegean Sea, Turkey. Kafkas Univ Vet Fak Derg, 18(5), 845-848.
6. Arizono N, Yamada M, Tegoshi T, Yoshikawa M (2012): Anisakis simplex sensu stricto and Anisakis pegreffii: biological characteristics and pathogenetic potential in human anisakiasis. Foodborne Pathog Dis, 9(6), 517-521.
7. Audicana L, Audicana MT, Fernandez de Corres L, Kennedy MW (1997): Cooking and freezing may not protect against allergenic reactions to ingested Anisakis simplex antigens in humans. Vet Rec, 140(9), 235.
8. Aytemiz I, Dede A, Danyer E, Tonay AM (2012): Morphological identification of parasites found in the stomach contents of bycaught striped dolphins (Stenella coeruleoalba) from Turkish Eastern Mediterranean Sea coast. J Black Sea/Mediterr, 18, 238-245.
9. Bao M, Pierce GJ, Strachan NJC, Pascual S (2019): Human health, legislative and socioeconomic issues caused by the fish-borne zoonotic parasites Anisakis: challenges in risk assessment. Trends Food Sci Tech, 86, 298-310.
10. Berland B (1961): Nematodes from some Norwegian marine fishes. Sarsia, 2, 1-50.
11. Bilecenoğlu M, Kaya M, Cihangir B, Çiçek E (2014): An updated checklist of marine fishes of Turkey. Turk J Zool, 38, 901-929.
12. Bilska-Zajac E, Różycki M, Chmurzyńska E, Karamon J, Sroka J, Kochanowski M, Kusyk P, Cencek T (2015): Parasites of Anisakidae family− geographical distribution and threat to human health. J Agr Sci Tech-Iran, 5, 146-152.
13. Buchmann K, Mehrdana F (2016): Effects of anisakid nematodes Anisakis simplex (s.l.) Pseudoterranova decipiens (s.l.) and Contracaecum osculatum (s.l.) on fish and consumer health. FAWPAR, 4, 13-22.
14. Cipriani P, Sbaraglia GL, Palomba M, Giulietti L, Bellisario B, Bušelić I, Mladineoc I, Cheleschid R, Nascetti G, Mattiucci S (2018): Anisakis pegreffii (Nematoda: Anisakidae) in European anchovy Engraulis encrasicolus from the Mediterranean Sea: Fishing ground as a predictor of parasite distribution. Fish Res, 202, 59-68.
15. Collette B, Boustany A, Carpenter KE, Di Natale A, Fox W, Graves J, Juan Jorda M, Kada O, Nelson R, Oxenford H (2011): Scomber scombrus. The IUCN Red List of Threatened Species 2011: e.T170354A6764313. Erişim adresi: http://dx.doi.org/10.2305/IUCN.UK.2011- 2.RLTS.T170354A6764313.en. Erişim tarihi: 28.07.2019
16. Da Fonseca MCG, Knoff M, Felizardo NN, Di Azevedo MIN, Torres EJL, Gomes DC, Iñiguez AM, De São Clemente SC (2016): Integrative taxonomy of Anisakidae and Raphidascarididae (Nematoda) in Paralichthys patagonicus and Xystreurys rasile (Pisces: Teleostei) from Brazil. Int J Food Microbiol, 235, 113-124.
17. Eissa AE, Showehdi ML, Ismail MM, El-Naas AS, Mhara, AAA, Abolghait SK (2018): Identification and prevalence of Anisakis pegreffii and A. pegreffii x A. Simplex (ss) hybrid genotype larvae in Atlantic horse mackerel (Trachurus trachurus) from some North African Mediterranean coasts. Egypt J Aquat Res, 44(1), 21-27.
18. Fish Base (2019): Scomber scombrus Linnaeus 1758, Atlantic mackerel. Erişim adresi: https://www.fishbase.se/Summary/SpeciesSummary.php?ID=118&AT=atlantic+mackerel Erişim tarihi: 27.07.2019
19. Keser R, Bray RA, Oğuz MC, Çelen S, Erdoğan S, Doğutürk S, Aklanoğlu G, Martı B (2007): Helminth parasites of digestive tract of some teleost fish caught in the Dardanelles at Çanakkale, Turkey. Helminthologia, 44 (4), 217-221.
20. Keskin E, Genc E, Unal EM (2013): Identification of Anisakis simplex using DNA barcoding. Aquaculture Europe 2013-Trondheim, Norway.
21. Mattiucci S, Nascetti G (2006): Molecular systematics, phylogeny and ecology of anisakid nematodes of the genus Anisakis Dujardin, 1845: an update. Parasite, 13(2), 99-113.
22. Mattiucci S, Nascetti G (2008): Advances and trends in the molecular systematics of anisakid nematodes, with implications for their evolutionary ecology and host—parasite co-evolutionary processes. Adv Parasitol, 66, 47-148.
23. Molina-Fernández D, Adroher FJ, Benítez R (2018): A scanning electron microscopy study of Anisakis physeteris molecularly identified: from third stage larvae from fish to fourth stage larvae obtained in vitro. Parasitol Res, 117, 2095–2103.
24. Orecchia P, Paggi L, Mattiucci S, Nascetti G, Smith JW, Bullini L (1986): Electrophoretic identification of larvae and adults of Anisakis (Ascaridida: Anisakidae). J Helminthol, 60, 331-339.
25. Pekmezci GZ, Onuk EE, Bolukbas CS, Yardimci B, Gurler AT, Acici M, Umur S (2014): Molecular identification of Anisakis species (Nematoda: Anisakidae) from marine fishes collected in Turkish waters. Vet Parasitol, 201, 82–94.
26. Pekmezci GZ, Yardimci B (2019): On the occurrence and molecular identification of Contracaecum larvae (Nematoda: Anisakidae) in Mugil cephalus from Turkish waters. Parasitol Res, 118(5), 1393-1402.
27. Pekmezci GZ, Yardimci B, Gurler AT, Bolubas CS, Acici M, Umur S (2013): Survey on the presence of nematodes and associated with pathology in marine mammals from Turkish waters. Kafkas Univ Vet Fak Derg, 19(6), 1035-1038.
28. Quiazon KMA, Yoshinaga T, Ogawa K, Yukami R (2008): Morphological differences between larvae and in vitro-cultured adults of Anisakis simplex (sensu stricto) and Anisakis pegreffii (Nematoda: Anisakidae). Parasitol Int, 57(4), 483-489.
29. Şimşek E, Yılmaz E (2016): Anisakiasis ve halk sağlığı. Turkiye Klinikleri J Food Hyg Technol-Special Topics, 202(3), 38-44.
30. Tepe Y, Oğuz MC (2013): Nematode and acanthocephalan parasites of marine fish of the eastern Black Sea coasts of Turkey. Turk J Zool, 37, 753-760.
31. Tüik (2019): Su ürünleri istatistikleri: Deniz ürünleri: Avlanan deniz balıkları miktarı. Erişim adresi: http://www.tuik.gov.tr/PreTablo.do?alt_id=1005 Erişim tarihi: 31.07.2019
32. Utuk AE, Piskin FC, Balkaya I (2012): Molecular detection of Anisakis pegreffii in Horse mackerels (Trachurus trachurus) sold for human consumption in Erzurum province of Turkey. Kafkas Univ Vet Fak Derg, 18(2), 303-307.
33. Veryeri NG (2012): Postmortem examinations of stranded dolphins found on the Black Sea coast near Ordu, Turkey (Mammalia: Cetacea). Zool Middle East, 55(1), 129-132.
34. Yardımcı B, Pekmezci GZ, Onuk EE (2014): Pathology and molecular identification of Anisakis pegreffii (Nematoda: Anisakidae) infection in the John Dory, Zeus faber (Linnaeus, 1758) caught in Mediterranean Sea. Ankara Univ Vet Fak Derg, 61, 233-236.

APA	özbakış g, aştı c, Gonenc B (2020). Anisakis spp. infection in Atlantic mackerel (Scomber scombrus, Linnaeus 1758) from the Sea of Marmara. , 80 - 85. 10.33188/vetheder.599455
Chicago	özbakış gökben,aştı ceren,Gonenc Bahadir Anisakis spp. infection in Atlantic mackerel (Scomber scombrus, Linnaeus 1758) from the Sea of Marmara. (2020): 80 - 85. 10.33188/vetheder.599455
MLA	özbakış gökben,aştı ceren,Gonenc Bahadir Anisakis spp. infection in Atlantic mackerel (Scomber scombrus, Linnaeus 1758) from the Sea of Marmara. , 2020, ss.80 - 85. 10.33188/vetheder.599455
AMA	özbakış g,aştı c,Gonenc B Anisakis spp. infection in Atlantic mackerel (Scomber scombrus, Linnaeus 1758) from the Sea of Marmara. . 2020; 80 - 85. 10.33188/vetheder.599455
Vancouver	özbakış g,aştı c,Gonenc B Anisakis spp. infection in Atlantic mackerel (Scomber scombrus, Linnaeus 1758) from the Sea of Marmara. . 2020; 80 - 85. 10.33188/vetheder.599455
IEEE	özbakış g,aştı c,Gonenc B "Anisakis spp. infection in Atlantic mackerel (Scomber scombrus, Linnaeus 1758) from the Sea of Marmara." , ss.80 - 85, 2020. 10.33188/vetheder.599455
ISNAD	özbakış, gökben vd. "Anisakis spp. infection in Atlantic mackerel (Scomber scombrus, Linnaeus 1758) from the Sea of Marmara". (2020), 80-85. https://doi.org/10.33188/vetheder.599455

APA	özbakış g, aştı c, Gonenc B (2020). Anisakis spp. infection in Atlantic mackerel (Scomber scombrus, Linnaeus 1758) from the Sea of Marmara. Veteriner Hekimler Derneği Dergisi, 91(1), 80 - 85. 10.33188/vetheder.599455
Chicago	özbakış gökben,aştı ceren,Gonenc Bahadir Anisakis spp. infection in Atlantic mackerel (Scomber scombrus, Linnaeus 1758) from the Sea of Marmara. Veteriner Hekimler Derneği Dergisi 91, no.1 (2020): 80 - 85. 10.33188/vetheder.599455
MLA	özbakış gökben,aştı ceren,Gonenc Bahadir Anisakis spp. infection in Atlantic mackerel (Scomber scombrus, Linnaeus 1758) from the Sea of Marmara. Veteriner Hekimler Derneği Dergisi, vol.91, no.1, 2020, ss.80 - 85. 10.33188/vetheder.599455
AMA	özbakış g,aştı c,Gonenc B Anisakis spp. infection in Atlantic mackerel (Scomber scombrus, Linnaeus 1758) from the Sea of Marmara. Veteriner Hekimler Derneği Dergisi. 2020; 91(1): 80 - 85. 10.33188/vetheder.599455
Vancouver	özbakış g,aştı c,Gonenc B Anisakis spp. infection in Atlantic mackerel (Scomber scombrus, Linnaeus 1758) from the Sea of Marmara. Veteriner Hekimler Derneği Dergisi. 2020; 91(1): 80 - 85. 10.33188/vetheder.599455
IEEE	özbakış g,aştı c,Gonenc B "Anisakis spp. infection in Atlantic mackerel (Scomber scombrus, Linnaeus 1758) from the Sea of Marmara." Veteriner Hekimler Derneği Dergisi, 91, ss.80 - 85, 2020. 10.33188/vetheder.599455
ISNAD	özbakış, gökben vd. "Anisakis spp. infection in Atlantic mackerel (Scomber scombrus, Linnaeus 1758) from the Sea of Marmara". Veteriner Hekimler Derneği Dergisi 91/1 (2020), 80-85. https://doi.org/10.33188/vetheder.599455