Expression of e-cadherin in early mouse embryo development
Yıl: 2020 Cilt: 91 Sayı: 1 Sayfa Aralığı: 9 - 14 Metin Dili: İngilizce DOI: 10.33188/vetheder.579594 İndeks Tarihi: 29-04-2021
Expression of e-cadherin in early mouse embryo development
Öz: E-cadherin is a member of the cadherin superfamily of calcium-dependent cell adhesion molecules, and has criticalroles in cell adhesion during early embryo development. To determine the expression and distribution of E-cadherin, weapplied immunofluorescent staining on late 8 cell, morula and blastocyst stage embryos during the experiments. Firstly,we superovulated the female mice and sacrificed them to isolate oviducts. We flushed 2 cell embryos by using FHMHEPES-buffered medium from the CD1 female mouse oviducts and cultured them in KSOM medium until theblastocyst stage. Embryos were fixed and immunostained with E-cadherin, then images were assessed using afluorescence microscope. We demonstrate that E-cadherin is expressed throughout the late 8 cell to fully expandedblastocysts. We observed that E-cadherin is present only in the cell-cell contact sites and not in the apical sites of themembrane of late 8 cell stage embryo. In morula, E-cadherin localized on the surface of blastomeres and cell-celljunctions. In blastocyst stage, embryos showed membrane staining in cell-cell contact regions in trophectoderm cellswhile localized on the surface of inner cell mass. Our results indicate that E-cadherin mediated cell adhesion hasimportant roles on compaction, trophectoderm epithelial differentiation and implantation.
Anahtar Kelime: Farelerde erken embriyo gelişiminde e-kadherin ekspresyonu
Öz: Kalsiyum bağımlı hücre adezyon moleküllerinden kadherin üst ailesinin bir üyesi olan E-kadherin’in erken embriyonik gelişim sırasında hücre adezyonunda kritik rolleri vardır. E-kadherin ekspresyonunu ve dağılımını belirlemek için, geç 8 hücre, morula ve blastosist aşamalarında bulunan embriyolara immünofloresan boyama uygulandı. İlk olarak dişi farelere superovulasyon uyguladıktan sonra sakrifiye edilerek oviduktar izole edildi. CD1 dişi farelerin oviduktları FHM HEPES-tamponlu medyum ile yıkanarak 2 hücreli embriyo elde edildi ve istenilen embriyo aşamalarında kullanılmak üzere KSOM medyumu içerisinde blastosist aşamasına kadar kültüre edildi. Embriyolar tespit edildi ve Ekadherin ile immun boyama yapıldıktan sonra, floresan mikroskobu kullanılarak görüntüler çekildi ve değerlendirildi. E-kadherinin geç 8 hücreli aşamadan tamamen genişletilmiş blastosist aşamaları boyunca eksprese edildiği gösterildi. Geç 8 hücreli embriyoda E-kadherin, membranın apikal bölgelerinde yer almazken sadece hücre-hücre temas bölgelerinde var olduğu gözlemlendi. Morulada E-kadherin, blastomerlerin yüzeyinde ve hücre-hücre birleşim alanlarında lokalize olduğu görüldü. Blastosist aşamasındaki embriyolarda, ICM'nin yüzeyinde lokalize olurken, TE hücrelerinde hücre-hücre temas bölgelerinde membran boyaması olduğu da gösterilmiştir. Sonuçlarımız, E-cadherin kaynaklı hücre adezyonunun, kompaksiyon, trofektoderm epitelyal farklılaşması ve implantasyon sürecinde önemli rollere sahip olduğunu göstermektedir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- 1. De Vries, WN, Evsikov AV, Haac BE, Fancher KS, Holbrook AE, Kemler R, Solter D, Knowles BB (2004): Maternal beta-catenin and Ecadherin in mouse development. Development, 131, 4435-4445.
- 2. Eckert JJ, Fleming TP (2008): Tight junction biogenesis during early development. Biochim Biophys Acta, 1778(3), 717-28.
- 3. Fleming TP, Sheth B, Fesenko I (2001): Cell adhesion in the preimplantation mammalian embryo and its role in trophectoderm differentiation and blastocyst morphogenesis. Front Biosci, 6, D1000-7.
- 4. Johnson MH, Maro B, Takeichi M (1986): The role of cell adhesion in the synchronisation and orientation of polarization in 8-cell mouse blastomeres. J Embryol Exp Morphol, 93, 239-255.
- 5. Larue L, Ohsugi M, Hirchenhain J, Kemler R (1994): Ecadherin null mutant embryos fail to form a trophectoderm epithelium. Proc. Natl Acad. Sci. USA, 91, 8263–8267.
- 6. Mihajlović AI, Bruce AW (2017): The first cell-fate decision of mouse preimplantation embryo development: integrating cell position and polarity. Open Biol, 7(11), pii: 170210. 70210.
- 7. Mutluay D (2016): Distribution of primitive endoderm and epiblast lineage specific factors in late stage blastocysts Slov Vet Res, 53 (4), 211-7
- 8. Mutluay D, Oner H (2017): The Abelson tyrosine kinase (c-Abl) localization in preimplantation mouse development. Rom J Morphol Embryol, 58, 1385-1391.
- 9. Niessen CM, Gottardi CJ (2008): Molecular components of the adherens junction. Biochim Biophys Acta, 1778(3), 562-71.
- 10.Ohsugi M, Hwang SY, Butz S, Knowles BB, Solter D, Kemler R (1996): Expression and cell membrane localization of catenins during mouse preimplantation development. Dev Dyn, 206(4), 391-402.
- 11.Ohsugi M, Larue L, Schwarz H, Kemler R (1997): Cell-junctional and cytoskeletal organization in mouse blastocysts lacking E-cadherin. Dev Biol, 185(2), 261-71.
- 12.Paria BC, Zhao X, Das SK, Dey SK, Yoshinaga K (1999): Zonula occludens-1 and E-cadherin are coordinately expressed in the mouse uterus with the initiation of implantation and decidualization. Dev Biol, 208(2), 488-501.
- 13.Piliszek A, Grabarek JB, Frankenberg SR, Plusa B (2016): Cell fate in animal and human blastocysts and the determination of viability. Mol Hum Reprod, 22(10), 681-690.
- 14. Riethmacher D, Brinkmann V, Birchmeier C (1995): A targeted mutation in the mouse E-cadherin gene results in defective preimplantation development. Proc. Natl Acad. Sci. USA, 92, 855–859.
- 15.Stephenson RO, Yamanaka Y, Rossant J (2010): Disorganized epithelial polarity and excess trophectoderm cell fate in preimplantation embryos lacking E-cadherin. Development, 137(20), 3383-91.
- 16.Vestweber D, Gossler A, Boller K, Kemler R (1987): Expression and distribution of cell adhesion molecule uvomorulin in mouse preimplantation embryos. Dev Biol, 124(2), 451-6.
- 17.Yamanaka Y, Ralston A, Stephenson RO, Rossant J (2006): Cell and molecular regulation of the mouse blastocyst. Dev Dyn. 235(9), 2301-14.
| APA | mutluay d (2020). Expression of e-cadherin in early mouse embryo development. , 9 - 14. 10.33188/vetheder.579594 |
| Chicago | mutluay duygu Expression of e-cadherin in early mouse embryo development. (2020): 9 - 14. 10.33188/vetheder.579594 |
| MLA | mutluay duygu Expression of e-cadherin in early mouse embryo development. , 2020, ss.9 - 14. 10.33188/vetheder.579594 |
| AMA | mutluay d Expression of e-cadherin in early mouse embryo development. . 2020; 9 - 14. 10.33188/vetheder.579594 |
| Vancouver | mutluay d Expression of e-cadherin in early mouse embryo development. . 2020; 9 - 14. 10.33188/vetheder.579594 |
| IEEE | mutluay d "Expression of e-cadherin in early mouse embryo development." , ss.9 - 14, 2020. 10.33188/vetheder.579594 |
| ISNAD | mutluay, duygu. "Expression of e-cadherin in early mouse embryo development". (2020), 9-14. https://doi.org/10.33188/vetheder.579594 |
| APA | mutluay d (2020). Expression of e-cadherin in early mouse embryo development. Veteriner Hekimler Derneği Dergisi, 91(1), 9 - 14. 10.33188/vetheder.579594 |
| Chicago | mutluay duygu Expression of e-cadherin in early mouse embryo development. Veteriner Hekimler Derneği Dergisi 91, no.1 (2020): 9 - 14. 10.33188/vetheder.579594 |
| MLA | mutluay duygu Expression of e-cadherin in early mouse embryo development. Veteriner Hekimler Derneği Dergisi, vol.91, no.1, 2020, ss.9 - 14. 10.33188/vetheder.579594 |
| AMA | mutluay d Expression of e-cadherin in early mouse embryo development. Veteriner Hekimler Derneği Dergisi. 2020; 91(1): 9 - 14. 10.33188/vetheder.579594 |
| Vancouver | mutluay d Expression of e-cadherin in early mouse embryo development. Veteriner Hekimler Derneği Dergisi. 2020; 91(1): 9 - 14. 10.33188/vetheder.579594 |
| IEEE | mutluay d "Expression of e-cadherin in early mouse embryo development." Veteriner Hekimler Derneği Dergisi, 91, ss.9 - 14, 2020. 10.33188/vetheder.579594 |
| ISNAD | mutluay, duygu. "Expression of e-cadherin in early mouse embryo development". Veteriner Hekimler Derneği Dergisi 91/1 (2020), 9-14. https://doi.org/10.33188/vetheder.579594 |
