Yıl: 2020 Cilt: 21 Sayı: 2 Sayfa Aralığı: 140 - 146 Metin Dili: İngilizce DOI: 10.4274/meandros.galenos.2020.55706 İndeks Tarihi: 06-05-2021

Evaluation of the Efficacy of 2%Chlorhexidine in Combination withPassive Ultrasonic Irrigation onEnterococcus faecalis Biofilm

Öz:
Objective: This study aimed to evaluate the combined effectiveness of 2% chlorhexidine (CHX) and passive ultrasonic irrigation (PUI) against Enterococcus faecalis (E. faecalis) biofilm. Materials and Methods: The root canals of 66 single-rooted extracted human mandibular premolar teeth (n=66) were enlarged up to a size of 40/0.06 taper and autoclaved. Fifty-nine roots were inoculated with E. faecalis and incubated for 4 weeks, whereas 7 roots served as the negative control group and were filled with sterile brain heart infusion broth. The remaining specimens (n=59) were assigned into 4 experimental groups (n=13) and a positive control group (n=7): CHX via standard needle irrigation (SNI), sodium hypochloride (NaOCl) via SNI, CHX via PUI, NaOCl via PUI and non-treated positive controls. Bacteriological samples were collected before and after the intervention, and microbiological analysis was performed by counting the colony forming units. Reduction in colony count (RCC) between before and after the intervention in each group were compared using One-Way ANOVA. Results: The highest RCC was determined in NaOCl/PUI and the lowest in CHX/SNI (p<0.05). Regarding RCC, CHX/PUI showed statistically similar results with NaOCl/PUI and NaOCl/SNI (p>0.05). Conclusion: A PUI of 2% CHX induced a statistically similar amount of RCC with both a PUI of 2.5% NaOCl and SNI of 2.5% NaOCl. PUI combined with 2% CHX can be used in secondary endodontic infections and during routine endodontic treatment due to increased antibacterial efficiency against E. faecalis
Anahtar Kelime:

%2’lik Klorheksidinin Pasif Ultrasonik İrrigasyon Eşliğinde Kullanımının Enterococcus faecalis Biyofilmi Üzerine Etkinliğinin Değerlendirilmesi

Öz:
Amaç: Bu çalışmanın amacı %2’lik klorheksidin (CHX) ve pasif ultrasonik irrigasyonun(PUİ) kombine kullanımının Enterococcus faecalis (E. faecalis) biyofilmine karşıetkinliğini değerlendirmektir.Gereç ve Yöntemler: Bu çalışmada 66 adet (n=66) tek köklü insan alt küçük azı dişine ait kök kanalları 40/0,06’lık genişlik vekonisiteye kadar şekillendirildi ve dişler otoklavlandı. Dişlerden 59 tanesi E. faecalis suşu ile enfekte edilerek 4 hafta inkübasyonabırakıldı (n=59), kalan 7 adet dişin kök kanalları steril beyin kalp infüzyon sıvı besiyeri ile dolduruldu ve negatif kontrol grubunaalındı (n=7). Elli dokuz adet diş, 4 deney grubu (n=13) ve 1 pozitif kontrol grubuna (n=7) ayrıldı. Standart şırınga irrigasyonu (SŞİ)/ CHX, SŞİ/Sodyum hipoklorit (NaOCl), PUİ/CHX, PUİ/NaOCl deney gruplarını oluşturdu ve pozitif kontrol grubuna irrigasyon işlemiuygulanmadı. İrrigasyon işleminden önce ve sonra olmak üzere bakteri örnekleri kök kanallarından toplandı ve kültürde gelişenkoloni oluşturan birimler sayıldı. Her grupta irrigasyon işleminin neden olduğu koloni cinsinden azalma (KCA) tek yönlü varyansanalizi kullanılarak karşılaştırıldı.Bulgular: En yüksek KCA, NaOCl/PUİ grubunda saptanırken en düşük KCA, CHX/SNI grubunda tespit edildi ve bu gruplar arasındaistatistiksel açıdan anlamlı fark gözlendi (p<0,05). KCA miktarları açısından CHX/PUİ grubunda, NaOCl/PUİ ve NaOCl/SŞİ grupları ileistatistiksel açıdan benzer sonuç elde edildi (p>0,05). Sonuç: %2’lik CHX solüsyonunun PUİ eşliğinde kullanımı; %2,5’lik NaOCl’nin PUİ ve SŞİ eşliğinde kullanımıyla istatistiksel açıdanbenzer KCA’ya neden olmuştur. %2’lik CHX solüsyonunun PUİ eşliğinde kullanımı E. faecalis’e karşı üstün antibakteriyel etkinliğinedeniyle ikincil endodontik enfeksiyonlarda ve rutin endodontik tedavi sırasında tercih edilebilir.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA KESİM B, Tezcan Ülger S, TOPÇUOĞLU H, ASLAN G, Kurnaz N, Sagsen B (2020). Evaluation of the Efficacy of 2%Chlorhexidine in Combination withPassive Ultrasonic Irrigation onEnterococcus faecalis Biofilm. , 140 - 146. 10.4274/meandros.galenos.2020.55706
Chicago KESİM Bertan,Tezcan Ülger Seda,TOPÇUOĞLU Hüseyin Sinan,ASLAN GÖNÜL,Kurnaz Nurbanu,Sagsen Burak Evaluation of the Efficacy of 2%Chlorhexidine in Combination withPassive Ultrasonic Irrigation onEnterococcus faecalis Biofilm. (2020): 140 - 146. 10.4274/meandros.galenos.2020.55706
MLA KESİM Bertan,Tezcan Ülger Seda,TOPÇUOĞLU Hüseyin Sinan,ASLAN GÖNÜL,Kurnaz Nurbanu,Sagsen Burak Evaluation of the Efficacy of 2%Chlorhexidine in Combination withPassive Ultrasonic Irrigation onEnterococcus faecalis Biofilm. , 2020, ss.140 - 146. 10.4274/meandros.galenos.2020.55706
AMA KESİM B,Tezcan Ülger S,TOPÇUOĞLU H,ASLAN G,Kurnaz N,Sagsen B Evaluation of the Efficacy of 2%Chlorhexidine in Combination withPassive Ultrasonic Irrigation onEnterococcus faecalis Biofilm. . 2020; 140 - 146. 10.4274/meandros.galenos.2020.55706
Vancouver KESİM B,Tezcan Ülger S,TOPÇUOĞLU H,ASLAN G,Kurnaz N,Sagsen B Evaluation of the Efficacy of 2%Chlorhexidine in Combination withPassive Ultrasonic Irrigation onEnterococcus faecalis Biofilm. . 2020; 140 - 146. 10.4274/meandros.galenos.2020.55706
IEEE KESİM B,Tezcan Ülger S,TOPÇUOĞLU H,ASLAN G,Kurnaz N,Sagsen B "Evaluation of the Efficacy of 2%Chlorhexidine in Combination withPassive Ultrasonic Irrigation onEnterococcus faecalis Biofilm." , ss.140 - 146, 2020. 10.4274/meandros.galenos.2020.55706
ISNAD KESİM, Bertan vd. "Evaluation of the Efficacy of 2%Chlorhexidine in Combination withPassive Ultrasonic Irrigation onEnterococcus faecalis Biofilm". (2020), 140-146. https://doi.org/10.4274/meandros.galenos.2020.55706
APA KESİM B, Tezcan Ülger S, TOPÇUOĞLU H, ASLAN G, Kurnaz N, Sagsen B (2020). Evaluation of the Efficacy of 2%Chlorhexidine in Combination withPassive Ultrasonic Irrigation onEnterococcus faecalis Biofilm. Meandros Medical And Dental Journal, 21(2), 140 - 146. 10.4274/meandros.galenos.2020.55706
Chicago KESİM Bertan,Tezcan Ülger Seda,TOPÇUOĞLU Hüseyin Sinan,ASLAN GÖNÜL,Kurnaz Nurbanu,Sagsen Burak Evaluation of the Efficacy of 2%Chlorhexidine in Combination withPassive Ultrasonic Irrigation onEnterococcus faecalis Biofilm. Meandros Medical And Dental Journal 21, no.2 (2020): 140 - 146. 10.4274/meandros.galenos.2020.55706
MLA KESİM Bertan,Tezcan Ülger Seda,TOPÇUOĞLU Hüseyin Sinan,ASLAN GÖNÜL,Kurnaz Nurbanu,Sagsen Burak Evaluation of the Efficacy of 2%Chlorhexidine in Combination withPassive Ultrasonic Irrigation onEnterococcus faecalis Biofilm. Meandros Medical And Dental Journal, vol.21, no.2, 2020, ss.140 - 146. 10.4274/meandros.galenos.2020.55706
AMA KESİM B,Tezcan Ülger S,TOPÇUOĞLU H,ASLAN G,Kurnaz N,Sagsen B Evaluation of the Efficacy of 2%Chlorhexidine in Combination withPassive Ultrasonic Irrigation onEnterococcus faecalis Biofilm. Meandros Medical And Dental Journal. 2020; 21(2): 140 - 146. 10.4274/meandros.galenos.2020.55706
Vancouver KESİM B,Tezcan Ülger S,TOPÇUOĞLU H,ASLAN G,Kurnaz N,Sagsen B Evaluation of the Efficacy of 2%Chlorhexidine in Combination withPassive Ultrasonic Irrigation onEnterococcus faecalis Biofilm. Meandros Medical And Dental Journal. 2020; 21(2): 140 - 146. 10.4274/meandros.galenos.2020.55706
IEEE KESİM B,Tezcan Ülger S,TOPÇUOĞLU H,ASLAN G,Kurnaz N,Sagsen B "Evaluation of the Efficacy of 2%Chlorhexidine in Combination withPassive Ultrasonic Irrigation onEnterococcus faecalis Biofilm." Meandros Medical And Dental Journal, 21, ss.140 - 146, 2020. 10.4274/meandros.galenos.2020.55706
ISNAD KESİM, Bertan vd. "Evaluation of the Efficacy of 2%Chlorhexidine in Combination withPassive Ultrasonic Irrigation onEnterococcus faecalis Biofilm". Meandros Medical And Dental Journal 21/2 (2020), 140-146. https://doi.org/10.4274/meandros.galenos.2020.55706