Pentilentetrazol ile Sıçanlarda Oluşturulan Deneysel Epilepsi Modelinde Oksolamin Sitratın Antikonvulsan Etkisi

erbas, oytun; Erdogan, Mumin

doi:10.22312/sdusbed.663909

Pentilentetrazol ile Sıçanlarda Oluşturulan Deneysel Epilepsi Modelinde Oksolamin Sitratın Antikonvulsan Etkisi

Mümin Alper ERDOĞAN, (İzmir Katip Çelebi Üniversitesi, Tıp Fakültesi, Fizyoloji Ana Bilim Dalı, İzmir, Türkiye)

Oytun ERBAŞ (Demiroğlu Bilim Üniversitesi, Tıp Fakültesi, Fizyoloji Ana Bilim Dalı, İstanbul, Türkiye)

Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi

12 1

Yıl: 2020 Cilt: 11 Sayı: 1 Sayfa Aralığı: 92 - 99 Metin Dili: Türkçe DOI: 10.22312/sdusbed.663909 İndeks Tarihi: 06-05-2021

Pentilentetrazol ile Sıçanlarda Oluşturulan Deneysel Epilepsi Modelinde Oksolamin Sitratın Antikonvulsan Etkisi

Öz:

Amaç: Dünya çapında milyonlarca kişi epilepsi hastasıolarak sorun yaşamaktadır ve hastaların %25'inde şu andamevcut bulunan antiepileptik ilaçlara karşı dirençli nöbetlergözlenmektedir. Bu nedenlerle, halen etkili ve tolere edilebilendaha fazla sayıda antiepileptik ilaca ihtiyaç duyulmaya devamedilmektedir. Oksolamin sitrat, pre-klinik verilere dayanarakantiepileptik aktiviteye sahip olabilen yaygın bir antitussifilaçtır.Materyal-Metot: Sıçanlar randomize bir şekildeintraperitoneal (i.p.) Oksolamin ile iki farklı dozda veplasebo şeklinde tedavi edildi ve daha sonrasında güçlübir nöbet indükleyici bileşik olan pentilentetrazole (PTZ)i.p. olarak maruz bırakıldı. Oksolaminin epilepsi için sıçanmodelimizde antiepileptik özelliklere sahip olup olmadığınıbelirlemede sıçanların hemen sonrasındaki nöbet aktivitesielektroensefalografi (EEG), Racine'nin konvülsiyonölçeği (RCS) ve ilk miyoklonik jerk (TFMJ) zamanı iledeğerlendirildi.Bulgular: Plasebo ile karşılaştırıldığında, her iki dozdaOksolamin, nöbet aktivitesini önemli ölçüde inhibe etti.Ortalama EEG spike dalga yüzdesi % 75,3'ten (plasebo)% 35,8'e (düşük doz, p<0,01) ve %28,6'ya (yüksek doz,p<0,0001) azaldı. RCS, ortalama 5,7'den (plasebo) 4,7'ye(düşük doz, p<0.001) ve 3,3'e (yüksek doz, p<0,0001) düştü.TFMJ ortalama 62,5s'den (plasebo), 177,5s'ye (düşük doz,p<0,001) ve 223,3s'ye (yüksek doz, p<0,0001) yükseldi.Sonuç: Yaygın bir antitussif ilaç olan Oksolamin sitrat,PTZ ile indüklenen status epileptikus sıçan modelinde nöbetaktivitesini baskılamaktadır. Refrakter epilepsi için devameden etkili yeni tedaviler bulma gereksinimi göz önünealındığında, oksolaminin antiepileptik olarak kullanılmaolasılığı daha ileri düzeyde araştırılmalıdır.

Anahtar Kelime:

Anticonvulsant Effect of Oxolamine Citrate in Pentylenetetrazole Induced Experimental Epilepsy Model in Rats

Öz:

Objective: Millions of individuals worldwide suffer from epilepsy, and up to 25% of patients have seizures that are resistant to currently available antiepileptic drugs. Hence, there continues to be a need for more seizure medications that are effective yet tolerable. Oxolamine citrate is an established antitussive drug that, based on preclinical data, may also have antiepileptic activity. Material-Method: We treated rats with either intraperitoneal (i.p.) Oxolamine citrate at two different doses or placebo in randomized fashion and then exposed them to i.p. pentylenetetrazol (PTZ), a potent seizure-inducing compound. We measured the rats’ subsequent seizure activity with electroencephalography (EEG), Racine’s convulsion scale (RCS) and time to first myoclonic jerk (TFMJ) to determine whether Oxolamine citrate has antiepileptic properties in our rat model for epilepsy. Results: When compared to placebo, Oxolamine at both doses significantly suppressed seizure activity. Mean EEG spike wave percentage score decreased from 75.3% (placebo) to 35.8% (lower dose, p<0.01) and 28.6% (higher dose, p<0.0001). RCS decreased from a mean of 5.7 (placebo) to 4.7 (lower dose, p<0.001) and 3.3 (higher dose, p<0.0001). TFMJ had increased from a mean of 62.5 s (placebo), to 177.5 s (lower dose, p<0.001) and 223.3 s (higher dose, p<0.0001). Conclusions: Oxolamine citrate, a common antitussive drug, suppresses seizure activity in rats with PTZ-induced status epilepticus. Given the ongoing need to find effective therapies for refractory epilepsy, the possibility of using oxolamine as an antiepileptic should be further explored.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Erdogan M, erbas o (2020). Pentilentetrazol ile Sıçanlarda Oluşturulan Deneysel Epilepsi Modelinde Oksolamin Sitratın Antikonvulsan Etkisi. , 92 - 99. 10.22312/sdusbed.663909
Chicago	Erdogan Mumin,erbas oytun Pentilentetrazol ile Sıçanlarda Oluşturulan Deneysel Epilepsi Modelinde Oksolamin Sitratın Antikonvulsan Etkisi. (2020): 92 - 99. 10.22312/sdusbed.663909
MLA	Erdogan Mumin,erbas oytun Pentilentetrazol ile Sıçanlarda Oluşturulan Deneysel Epilepsi Modelinde Oksolamin Sitratın Antikonvulsan Etkisi. , 2020, ss.92 - 99. 10.22312/sdusbed.663909
AMA	Erdogan M,erbas o Pentilentetrazol ile Sıçanlarda Oluşturulan Deneysel Epilepsi Modelinde Oksolamin Sitratın Antikonvulsan Etkisi. . 2020; 92 - 99. 10.22312/sdusbed.663909
Vancouver	Erdogan M,erbas o Pentilentetrazol ile Sıçanlarda Oluşturulan Deneysel Epilepsi Modelinde Oksolamin Sitratın Antikonvulsan Etkisi. . 2020; 92 - 99. 10.22312/sdusbed.663909
IEEE	Erdogan M,erbas o "Pentilentetrazol ile Sıçanlarda Oluşturulan Deneysel Epilepsi Modelinde Oksolamin Sitratın Antikonvulsan Etkisi." , ss.92 - 99, 2020. 10.22312/sdusbed.663909
ISNAD	Erdogan, Mumin - erbas, oytun. "Pentilentetrazol ile Sıçanlarda Oluşturulan Deneysel Epilepsi Modelinde Oksolamin Sitratın Antikonvulsan Etkisi". (2020), 92-99. https://doi.org/10.22312/sdusbed.663909

APA	Erdogan M, erbas o (2020). Pentilentetrazol ile Sıçanlarda Oluşturulan Deneysel Epilepsi Modelinde Oksolamin Sitratın Antikonvulsan Etkisi. Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi, 11(1), 92 - 99. 10.22312/sdusbed.663909
Chicago	Erdogan Mumin,erbas oytun Pentilentetrazol ile Sıçanlarda Oluşturulan Deneysel Epilepsi Modelinde Oksolamin Sitratın Antikonvulsan Etkisi. Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi 11, no.1 (2020): 92 - 99. 10.22312/sdusbed.663909
MLA	Erdogan Mumin,erbas oytun Pentilentetrazol ile Sıçanlarda Oluşturulan Deneysel Epilepsi Modelinde Oksolamin Sitratın Antikonvulsan Etkisi. Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi, vol.11, no.1, 2020, ss.92 - 99. 10.22312/sdusbed.663909
AMA	Erdogan M,erbas o Pentilentetrazol ile Sıçanlarda Oluşturulan Deneysel Epilepsi Modelinde Oksolamin Sitratın Antikonvulsan Etkisi. Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi. 2020; 11(1): 92 - 99. 10.22312/sdusbed.663909
Vancouver	Erdogan M,erbas o Pentilentetrazol ile Sıçanlarda Oluşturulan Deneysel Epilepsi Modelinde Oksolamin Sitratın Antikonvulsan Etkisi. Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi. 2020; 11(1): 92 - 99. 10.22312/sdusbed.663909
IEEE	Erdogan M,erbas o "Pentilentetrazol ile Sıçanlarda Oluşturulan Deneysel Epilepsi Modelinde Oksolamin Sitratın Antikonvulsan Etkisi." Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi, 11, ss.92 - 99, 2020. 10.22312/sdusbed.663909
ISNAD	Erdogan, Mumin - erbas, oytun. "Pentilentetrazol ile Sıçanlarda Oluşturulan Deneysel Epilepsi Modelinde Oksolamin Sitratın Antikonvulsan Etkisi". Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi 11/1 (2020), 92-99. https://doi.org/10.22312/sdusbed.663909