Comparison of reverse-transcriptase polymerase chain reaction (RT-PCR) and rapid test for the detection of bovine rotavirus and bovine coronavirus in anatolian water buffaloes
Yıl: 2020 Cilt: 36 Sayı: 3 Sayfa Aralığı: 159 - 165 Metin Dili: İngilizce DOI: 10.15312/EurasianJVetSci.2020.273 İndeks Tarihi: 07-05-2021
Comparison of reverse-transcriptase polymerase chain reaction (RT-PCR) and rapid test for the detection of bovine rotavirus and bovine coronavirus in anatolian water buffaloes
Öz: Aim: Coronaviruses and Rotaviruses are important virologic factorsfor both animal and human health in Turkey and the world. Bovine Rotavirus (BRV) and Bovine Coronavirus (BCoV) in cattle causesignificant economic losses. The aim of this study was to determinethe presence of BRV and BCoV in Anatolian buffaloes which were onthe same farms with cattle. For this purpose, presence of these twoviruses were investigated by Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) and BRV-BCoV Rapid tests and sensitivity andspecificity ratios of these two tests were compared.Materials and Methods: In this study, 230 Anatolian buffaloes wereclinically evaluated in cattle farms in Afyonkarahisar region. Fecalsamples were collected from 27 buffaloes which had clinical signs(weakness, dehydration, vomiting, watery consistency and yellowstool). The fecal samples were evaluated by Rapid Test and RT-PCRfor Bovine Rotavirus and Bovine Coronavirus. The analyzes wereperformed according to the procedure of the commercial RT-PCRand rapid kits.Results: The RT-PCR results were positive as 22.2% (6/27) for BRVand 3.7% (1/27 27/1)for BcoV while Rota-Corona Rapid test resultswere negative in all samples. When compared with RT-PCR resultsfor both viruses, the rapid test sensitivity and specificity was determined as 0% and 100%, respectively. In addition, positive rates ofBRV was statistically important as BCoV rate in analyzed samples(p<0,05).Conclusion: In conclusion, low sensitivity of rapid test may be dueto the change in the amount of virus scattered throughout the courseof enteric infections.
Anahtar Kelime: Anadolu mandalarında bovine rotavirus ve bovine coronavirus tespitinde revers transkriptaz polimeraz zincir reaksiyonu (RT-PCR) ve hızlı testin karşılaştırıl
Öz: Amaç: Coronaviruslar ve Rotaviruslar, Türkiye’de ve Dünya’da hay van ve insan sağlığını tehdit eden önemli virolojik etkenlerdir. Sığır larda görülen Bovine Rotavirus (BRV) ve Bovine Coronavirus (BCoV) önemli ekonomik kayıplara yol açmaktadır. Bu çalışmada sığırlar ile aynı çiftlikte bulunan Anadolu mandalarında BRV ve BCoV varlığının teşhis edilmesi amaçlanmıştır. Bu amaçla iki virusun varlığı Revers Transkriptaz Polimeraz Zincir Reaksiyonu (RT-PCR) ve BRV-BCoV hızlı testi ile tespit edildi. Bu iki testin sensitivite ve spesifite oran ları karşılaştırıldı. Gereç ve Yöntem: Bu çalışmada Afyonkarahisar bölgesindeki sı ğır çiftliklerinde bulunan 230 adet Anadolu mandası klinik olarak değerlendirildi. Yirmi yedi adet klinik belirti (zayıflık, dehidrasyon, kusma, sulu kıvam ve sarı renkli dışkı) gösteren mandadan gaita nu muneleri toplandı. Gaita numuneleri BRV ve BCoV yönünden Hızlı test ve RT-PCR ile değerlendirildi. Belirtilen analizler, kullanılan tica ri kitlerin prosedürüne uygun olarak gerçekleştirildi. Bulgular: RT-PCR sonucunda %22.2 (27/6) BRV ve % 3.7 (27/1) oranında BCoV pozitiflik tespit edilirken, Rota-Corona Hızlı test le tüm numuneler negatif belirlendi. Her iki etken için rapid testin RT-PCR ile karşılaştırıldığında sensitivite ve spesifitesi sırayla %0 ve %100 olarak belirlendi. Ayrıca analiz edilen örneklerde istatis tiki olarak BRV pozitiflik oranı, BCoV’a göre daha önemli bulundu (p<0,05). Öneri: Sonuç olarak rapid test sensitivitesinin düşük çıkması, ente rik enfeksiyonların seyri boyunca saçılan virus miktarındaki değişik liğe bağlı olabilir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Bulut O, UYUNMAZ SAKLI G, HASOKSÜZ M, DİK I, HADIMLI H, HİTİT M (2020). Comparison of reverse-transcriptase polymerase chain reaction (RT-PCR) and rapid test for the detection of bovine rotavirus and bovine coronavirus in anatolian water buffaloes. , 159 - 165. 10.15312/EurasianJVetSci.2020.273 |
| Chicago | Bulut Oya,UYUNMAZ SAKLI Gülşah,HASOKSÜZ MUSTAFA,DİK IRMAK,HADIMLI HASAN HÜSEYIN,HİTİT Mustafa Comparison of reverse-transcriptase polymerase chain reaction (RT-PCR) and rapid test for the detection of bovine rotavirus and bovine coronavirus in anatolian water buffaloes. (2020): 159 - 165. 10.15312/EurasianJVetSci.2020.273 |
| MLA | Bulut Oya,UYUNMAZ SAKLI Gülşah,HASOKSÜZ MUSTAFA,DİK IRMAK,HADIMLI HASAN HÜSEYIN,HİTİT Mustafa Comparison of reverse-transcriptase polymerase chain reaction (RT-PCR) and rapid test for the detection of bovine rotavirus and bovine coronavirus in anatolian water buffaloes. , 2020, ss.159 - 165. 10.15312/EurasianJVetSci.2020.273 |
| AMA | Bulut O,UYUNMAZ SAKLI G,HASOKSÜZ M,DİK I,HADIMLI H,HİTİT M Comparison of reverse-transcriptase polymerase chain reaction (RT-PCR) and rapid test for the detection of bovine rotavirus and bovine coronavirus in anatolian water buffaloes. . 2020; 159 - 165. 10.15312/EurasianJVetSci.2020.273 |
| Vancouver | Bulut O,UYUNMAZ SAKLI G,HASOKSÜZ M,DİK I,HADIMLI H,HİTİT M Comparison of reverse-transcriptase polymerase chain reaction (RT-PCR) and rapid test for the detection of bovine rotavirus and bovine coronavirus in anatolian water buffaloes. . 2020; 159 - 165. 10.15312/EurasianJVetSci.2020.273 |
| IEEE | Bulut O,UYUNMAZ SAKLI G,HASOKSÜZ M,DİK I,HADIMLI H,HİTİT M "Comparison of reverse-transcriptase polymerase chain reaction (RT-PCR) and rapid test for the detection of bovine rotavirus and bovine coronavirus in anatolian water buffaloes." , ss.159 - 165, 2020. 10.15312/EurasianJVetSci.2020.273 |
| ISNAD | Bulut, Oya vd. "Comparison of reverse-transcriptase polymerase chain reaction (RT-PCR) and rapid test for the detection of bovine rotavirus and bovine coronavirus in anatolian water buffaloes". (2020), 159-165. https://doi.org/10.15312/EurasianJVetSci.2020.273 |
| APA | Bulut O, UYUNMAZ SAKLI G, HASOKSÜZ M, DİK I, HADIMLI H, HİTİT M (2020). Comparison of reverse-transcriptase polymerase chain reaction (RT-PCR) and rapid test for the detection of bovine rotavirus and bovine coronavirus in anatolian water buffaloes. Eurasian Journal of Veterinary Sciences, 36(3), 159 - 165. 10.15312/EurasianJVetSci.2020.273 |
| Chicago | Bulut Oya,UYUNMAZ SAKLI Gülşah,HASOKSÜZ MUSTAFA,DİK IRMAK,HADIMLI HASAN HÜSEYIN,HİTİT Mustafa Comparison of reverse-transcriptase polymerase chain reaction (RT-PCR) and rapid test for the detection of bovine rotavirus and bovine coronavirus in anatolian water buffaloes. Eurasian Journal of Veterinary Sciences 36, no.3 (2020): 159 - 165. 10.15312/EurasianJVetSci.2020.273 |
| MLA | Bulut Oya,UYUNMAZ SAKLI Gülşah,HASOKSÜZ MUSTAFA,DİK IRMAK,HADIMLI HASAN HÜSEYIN,HİTİT Mustafa Comparison of reverse-transcriptase polymerase chain reaction (RT-PCR) and rapid test for the detection of bovine rotavirus and bovine coronavirus in anatolian water buffaloes. Eurasian Journal of Veterinary Sciences, vol.36, no.3, 2020, ss.159 - 165. 10.15312/EurasianJVetSci.2020.273 |
| AMA | Bulut O,UYUNMAZ SAKLI G,HASOKSÜZ M,DİK I,HADIMLI H,HİTİT M Comparison of reverse-transcriptase polymerase chain reaction (RT-PCR) and rapid test for the detection of bovine rotavirus and bovine coronavirus in anatolian water buffaloes. Eurasian Journal of Veterinary Sciences. 2020; 36(3): 159 - 165. 10.15312/EurasianJVetSci.2020.273 |
| Vancouver | Bulut O,UYUNMAZ SAKLI G,HASOKSÜZ M,DİK I,HADIMLI H,HİTİT M Comparison of reverse-transcriptase polymerase chain reaction (RT-PCR) and rapid test for the detection of bovine rotavirus and bovine coronavirus in anatolian water buffaloes. Eurasian Journal of Veterinary Sciences. 2020; 36(3): 159 - 165. 10.15312/EurasianJVetSci.2020.273 |
| IEEE | Bulut O,UYUNMAZ SAKLI G,HASOKSÜZ M,DİK I,HADIMLI H,HİTİT M "Comparison of reverse-transcriptase polymerase chain reaction (RT-PCR) and rapid test for the detection of bovine rotavirus and bovine coronavirus in anatolian water buffaloes." Eurasian Journal of Veterinary Sciences, 36, ss.159 - 165, 2020. 10.15312/EurasianJVetSci.2020.273 |
| ISNAD | Bulut, Oya vd. "Comparison of reverse-transcriptase polymerase chain reaction (RT-PCR) and rapid test for the detection of bovine rotavirus and bovine coronavirus in anatolian water buffaloes". Eurasian Journal of Veterinary Sciences 36/3 (2020), 159-165. https://doi.org/10.15312/EurasianJVetSci.2020.273 |
