Tavuk eti örneklerinde genişlemiş spektrumlu beta-laktamaz üreten Escherichia coli
Yıl: 2020 Cilt: 36 Sayı: 3 Sayfa Aralığı: 187 - 192 Metin Dili: Türkçe DOI: 10.15312/EurasianJVetSci.2020.277 İndeks Tarihi: 07-05-2021
Tavuk eti örneklerinde genişlemiş spektrumlu beta-laktamaz üreten Escherichia coli
Öz: Amaç: Bu çalışmada ticari olarak satışa sunulan ambalajlı ve am balajsız tavuk but, göğüs ve kanat etlerinde genişlemiş spektrumlu β-laktamaz (GSBL) üreten Escherichia coli prevalansını tespit etmek amaçlandı. Gereç ve Yöntem: Çalışmada, farklı marketlerde satışa sunulan 30 tavuk but, 30 tavuk göğüs ve 30 tavuk kanat örneği materyal olarak kullanıldı. Her bir numuneden, ön zenginleştirme sonrası Brillance ESBL agara ekimler yapılmış, uygun inkübasyonun ardından üre yen şüpheli kolonilerden max. 5 tanesi seçilerek, EMB agara geçildi. İzolatların E.coli olarak identifikasyonunda IMViC testler kullanıldı. Suşların GSBL üretim özellikleri Klinik ve Laboratuvar Standartları Enstitüsü (CLSI)’nce belirtilen kombine disk difüzyon yöntemi ile fenotopik olarak belirlendi. Bulgular: Tavuk eti örneklerinden elde edilen 159 E. coli şüpheli izolattan, 152 tanesi (%95,5) E.coli olarak belirlendi. Kombine disk difüzyon testi sonrasında 28 (%18,4) izolatın GSBL pozitif olduğu tespit edildi. İncelenen göğüs etlerinde 17 (%60), but örneklerinde 9 (%32) ve kanat örneklerinde de 2 (%7) adet GSBL izolatı bulundu. GSBL üreten E.coli izolatlarının ambalajlı ve açık örneklerdeki dağılı mı, sırasıyla %71 ve %28 olarak belirlendi. Öneri: Tavuk etlerinde GSBL üreten E. coli varlığı, bu tip direncin insanlara aktarımında gıdaların da önemli bir kaynak olabileceğini ortaya koymaktadır. Gıdalar düzenli olarak patojen bakteriler kapsa mında incelenmesine rağmen, GSBL üreten bakteriler bu kapsamda değildir. Bu durum gıda zinciri vasıtasıyla yeni dirençli bakterilerin fark edilmeden artmasına neden olabilmektedir. Bu nedenle bakteri lerde fenotipik olarak direncin belirlenmesinin yanı sıra, elde edilen izolatların ileri analizlerle genotipik olarak da bu özelliği taşıyıp ta şımadığının tespit edilmesi önerilmektedir.
Anahtar Kelime: Determination of extended spectrum beta-lactamase producing Escherichia coli strains in chicken meat samples
Öz: Aim: The aim of this study was to determine the prevalence of ex tended spectrum β-lactamase (ESBL) producing E.coli in retailed chicken thigh, breast and wing meats, with and without packaging. Materials and Methods: In the study, retailed 30 chicken thigh, 30 chicken breast and 30 chicken wing samples, were used as materials. After pre-enrichment, each sample was plated to the Brillance ESBL medium and incubated. Up to 5 suspected colonies were selected and plated to the EMB medium. Then the isolates were identified as E. coli by IMViC test. In order to determine the ESBL production of the isolates phenotypically combined disc diffusion test was perfor med according to the CSLI. Results: From the 159 suspicious isolates, 152 (95.5%) of them identified as E.coli. According to combined disc diffusion test 28 (18.4%) of the isolates were ESBL positive. 17 (60%) ESBL produ cing isolates were found in the breast meat samples while 9 (32%) isolates from thigh and 2 (7%) isolates from wings were positive for ESBL production. The distribution of ESBL producing E. coli isolatesin packaged and unpackaged samples were determined as 71% and 28%, respectively. Conclusion: The presence of ESBL producing E. coli in chicken meats indicates that foods may also be an important source for the trans mission of this resistance to human. Although foods are regularly examined in the context of pathogenic bacteria, bacteria producing GSBL are not covered by this. For this reason, it is suggested to de termine the isolates genotypically about this resistance by an
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- Buynak JD, 2006.Understanding the longevity ofthe β-lactam antibiotics and of antibiotic/β-lactamase inhibitor combinations. Biochem Pharmacol, 71(7), 930-940.
- Bitrus AA, Mshelia PA, Kwoji ID, Goni MD, et al., 2019. Extended-spectrum beta-lactamase and ampicillin class C betalactamase-producing Escherichia coli from food animals: a review. Int J One Health, 5, 65-75.
- Buess S, Zurfluh K, Stephan R, Guldimann C, 2019. Quantitative microbiological slaughter process analysis in a largescale Swiss poultry abattoir. Food Control, 105, 86-93.
- Campos CB, Fenner I, Wiese N, Lensing C, et al., 2014. Prevalence and genotypes of extended spectrum beta-lactamases in Enterobacteriaceae isolated from human stool and chicken meat in Hamburg, Germany. Int J Medical Microbiol, 304(5-6), 678-684.
- CLSI, 2013. Performance standards for antimicrobial susceptibility testing: twenty-third informational supplement; M100-S23. Clinical and Laboratory Standards Institute, Volume 33 Number 1, Wayne, PA, USA.
- Costa D, Vinue L, Poeta P, Coelho AC, et al., 2009. Prevalence of extended spectrum beta-lactamase-producing Escherichia coli isolates in faecal samples of broilers. Vet Microbiol, 138, 339-344.
- Dhanji H, Murphy NM, Doumith M, Durmus S, et al., 2005. Cephalosporin resistance mechanisms in Escherichia coli isolated from raw chicken imported into the UK. J Antimicrob Chemother, 65, 2534-2537.
- ECDC, 2017. Antimicrobial resistance surveillance in Europe 2015. Annual Report of the European Antimicrobial Resistance Surveillance Network (EARS-Net).
- EFSA, 2011. Scientific opinion on the public health risks of bacterial strains producing extended-spectrum β-lactamases and/or AmpC β-lactamases in food and food-producing animals. EFSA Panel on Biological Hazards.
- EFSA J, 9 (2011), 2322. Ghodousi A, Bonura C, Di Noto AM, Mammina C, 2015. Extended-spectrum ß-lactamase, AmpC-producing, and fluoroquinolone-resistant Escherichia coli in retail broiler chicken meat, Italy. Foodborne Pathog Dis, 12(7), 619-625.
- Giamarellou H, 2005. Multidrug resistance in Gram‐negative bacteria that produce extended‐spectrum β‐lactamases (ESBLs). Clin Microbiol Infect, 11, 1-16.
- Giuriatti J, Stefani LM, Brisola MC, Crecencio RB, et al., 2017. Salmonella Heidelberg: Genetic profile of its antimicrobial resistance related to extended spectrum β-lactamases (ESBLs). Microbiol Pathog, 109, 195-199.
- Huizinga P, Kluytmans-van den Bergh M, Rossen JW, Willemsen I ,et al., 2019. Decreasing prevalence of contamination with extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-E) in retail chicken meat in the Netherlands. PloS One, 14(12).1-17.
- Kaesbohrer A, Bakran-Lebl K, Irrgang A, Fischer J, et al., 2019. Diversity in prevalence and characteristics of ESBL/ pAmpC producing E. coli in food in Germany. Vet Microbiol, 233, 52-60.
- Leverstein-van Hall MA, Dierikx CM, Cohen Stuart J, Voets GM, et al., 2011. Dutch patients, retail chicken meat and poultry share the same ESBL genes, plasmids and strains. Clin Microbiol Infect, 17, 873-880.
- Ojo OE, Schwarz S, Michael GB, 2016. Detection and characterization of extended-spectrum β-lactamase-producing Escherichia coli from chicken production chains in Nigeria. Vet Microbiol, 194, 62-68.
- Päivärinta M, Latvio S, Fredriksson-Ahomaa M, Heikinheimo A, 2020. Whole genome sequence analysis of antimicrobial resistance genes, multilocus sequence types and plasmid sequences in ESBL/AmpC Escherichia coli isolated from broiler caecum and meat. Int J Food Microbiol, 315, 108361.
- Petternel C, Galler H, Zarfel G, Luxner J, et al., 2014. Isolation and characterization of multidrug-resistant bacteria from minced meat in Austria. Food Microbiol, 44, 41-46.
- Projahn M, Von Tippelskirch P, Semmler T, Guenther S, et al., 2019. Contamination of chicken meat with extendedspectrum beta-lactamase producing-Klebsiella pneumoniae and Escherichia coli during scalding and defeathering of broiler carcasses. Food Microbiol, 77, 185-191.
- Randall, LP, Clouting C, Horton RA, Coldham NG, et al., 2011. Prevalence of Escherichia coli carrying extended-spectrum betalactamases (CTX-M and TEM-52) from broiler chickens and turkeys in Great Britain between 2006 and 2009. J Antimicrob Chemother 66, 86-95.
- Randall LP, Lodge MP, Elviss NC, Lemma FL, et al., 2017. Evaluation of meat, fruit and vegetables from retail stores in five United Kingdom regions as sources of extended-spectrum beta-lactamase (ESBL)-producing and carbapenemresistant Escherichia coli. Int J Food Microbiol, 241, 283- 290.
- Sheikhi S, Ahmadi E, 2018. Phenotypic and molecular identification of extended-spectrum beta-lactamases in Escherichia coli in healthy broilers at the west region of Iran Eurasian J Vet Sci, 34(3), 178-184.
- Smet A, Martel A, Persoons D, Dewulf J, et al., 2008. Diversity of extended-spectrum-beta-lactamases and class C beta-lactamases among cloacal Escherichia coli isolates in Belgian broiler farms. Antimicrob Agents Chemother, 52, 1238-1243.
- Stuart JC, Van den Munckhof T., Voets G, Scharringa JF et al., 2012. Comparison of ESBL contamination in organic and conventional retail chicken meat. Int J Food Microbiol, 154(3), 212-214.
- Ungureanu V, Corcionivoschi N, Gundogdu O, Stef L, et al., 2019. The emergence of beta-lactamase producing Escherichia coli and the problems in assessing their potential contribution to foodborne illness: a review. AgroLife Sci J, 8(1), 248-260.
- Warren RE, Ensor VM, O'Neill P, Butler V, et al., 2008. Imported chickenmeat as a potential source of quinoloneresistant Escherichia coli producing extended-spectrumbeta-lactamases in the UK. J Antimicrob Chemother, 61, 504-508
| APA | IPLIKCIOGLU ARAL G, Cengiz G, Arslan B, ŞİRELİ T (2020). Tavuk eti örneklerinde genişlemiş spektrumlu beta-laktamaz üreten Escherichia coli. , 187 - 192. 10.15312/EurasianJVetSci.2020.277 |
| Chicago | IPLIKCIOGLU ARAL Güzin,Cengiz Görkem,Arslan Buse,ŞİRELİ Tansel Tavuk eti örneklerinde genişlemiş spektrumlu beta-laktamaz üreten Escherichia coli. (2020): 187 - 192. 10.15312/EurasianJVetSci.2020.277 |
| MLA | IPLIKCIOGLU ARAL Güzin,Cengiz Görkem,Arslan Buse,ŞİRELİ Tansel Tavuk eti örneklerinde genişlemiş spektrumlu beta-laktamaz üreten Escherichia coli. , 2020, ss.187 - 192. 10.15312/EurasianJVetSci.2020.277 |
| AMA | IPLIKCIOGLU ARAL G,Cengiz G,Arslan B,ŞİRELİ T Tavuk eti örneklerinde genişlemiş spektrumlu beta-laktamaz üreten Escherichia coli. . 2020; 187 - 192. 10.15312/EurasianJVetSci.2020.277 |
| Vancouver | IPLIKCIOGLU ARAL G,Cengiz G,Arslan B,ŞİRELİ T Tavuk eti örneklerinde genişlemiş spektrumlu beta-laktamaz üreten Escherichia coli. . 2020; 187 - 192. 10.15312/EurasianJVetSci.2020.277 |
| IEEE | IPLIKCIOGLU ARAL G,Cengiz G,Arslan B,ŞİRELİ T "Tavuk eti örneklerinde genişlemiş spektrumlu beta-laktamaz üreten Escherichia coli." , ss.187 - 192, 2020. 10.15312/EurasianJVetSci.2020.277 |
| ISNAD | IPLIKCIOGLU ARAL, Güzin vd. "Tavuk eti örneklerinde genişlemiş spektrumlu beta-laktamaz üreten Escherichia coli". (2020), 187-192. https://doi.org/10.15312/EurasianJVetSci.2020.277 |
| APA | IPLIKCIOGLU ARAL G, Cengiz G, Arslan B, ŞİRELİ T (2020). Tavuk eti örneklerinde genişlemiş spektrumlu beta-laktamaz üreten Escherichia coli. Eurasian Journal of Veterinary Sciences, 36(3), 187 - 192. 10.15312/EurasianJVetSci.2020.277 |
| Chicago | IPLIKCIOGLU ARAL Güzin,Cengiz Görkem,Arslan Buse,ŞİRELİ Tansel Tavuk eti örneklerinde genişlemiş spektrumlu beta-laktamaz üreten Escherichia coli. Eurasian Journal of Veterinary Sciences 36, no.3 (2020): 187 - 192. 10.15312/EurasianJVetSci.2020.277 |
| MLA | IPLIKCIOGLU ARAL Güzin,Cengiz Görkem,Arslan Buse,ŞİRELİ Tansel Tavuk eti örneklerinde genişlemiş spektrumlu beta-laktamaz üreten Escherichia coli. Eurasian Journal of Veterinary Sciences, vol.36, no.3, 2020, ss.187 - 192. 10.15312/EurasianJVetSci.2020.277 |
| AMA | IPLIKCIOGLU ARAL G,Cengiz G,Arslan B,ŞİRELİ T Tavuk eti örneklerinde genişlemiş spektrumlu beta-laktamaz üreten Escherichia coli. Eurasian Journal of Veterinary Sciences. 2020; 36(3): 187 - 192. 10.15312/EurasianJVetSci.2020.277 |
| Vancouver | IPLIKCIOGLU ARAL G,Cengiz G,Arslan B,ŞİRELİ T Tavuk eti örneklerinde genişlemiş spektrumlu beta-laktamaz üreten Escherichia coli. Eurasian Journal of Veterinary Sciences. 2020; 36(3): 187 - 192. 10.15312/EurasianJVetSci.2020.277 |
| IEEE | IPLIKCIOGLU ARAL G,Cengiz G,Arslan B,ŞİRELİ T "Tavuk eti örneklerinde genişlemiş spektrumlu beta-laktamaz üreten Escherichia coli." Eurasian Journal of Veterinary Sciences, 36, ss.187 - 192, 2020. 10.15312/EurasianJVetSci.2020.277 |
| ISNAD | IPLIKCIOGLU ARAL, Güzin vd. "Tavuk eti örneklerinde genişlemiş spektrumlu beta-laktamaz üreten Escherichia coli". Eurasian Journal of Veterinary Sciences 36/3 (2020), 187-192. https://doi.org/10.15312/EurasianJVetSci.2020.277 |
