Objective: Exposure to needlestick and sharps injuries (NSI) is among the major occupational risks of healthworkers. Yearly 385,000 NSI are reported by health workers. In this study, we aimed to evaluate the frequencyof NSI among nurses at Atatürk University Research Hospital together with the elaboration of the utilizationof effective preventive measures used for infection control as well as the affecting factors.Methods: All 562 nurses working at Atatürk University Research Hospital during September-November 2016were invited to fill a questionnaire with 27 items. Questions were asked about the sociodemographiccharacteristics,having experienced NSI, usage of protective equipment, and hepatitis B vaccination status. Atotal of 555 (98.7%) nurses volunteered to participate. Verbal consent was taken from the participants.Results: Of the participants, 425 (76.6%) were females, and 130 (23.4%) were males. Their mean age was27.5 ± 7.4 years. 52.6% (n = 293) of the participants were in the surgical clinics while 47.4% (n = 262) werein the medical clinics. Of the participants, 21.6% (n = 120) stated that they experienced a needlestick or sharpsinjury within the last one year. Of those injuries, 16.7% (n = 20) happened while removing the needle tip fromthe syringe, 29.2% (n=35) while trying to reattach the needle cap, 16.7% (n = 20) while filling the syringe, and12.5% (n = 15) while drawing blood from the patient.Conclusion: Health workers should be trained on injuries and all other occupational risks with cutting andpuncturing tools, and they should be instructed on the use of protective equipment.