Erol BASUGUY
(Dicle Üniversitesi, Tıp Fakültesi, Çocuk Cerrahisi Anabilim Dalı, Diyarbakır, Türkiye)
Mehmet Hanifi OKUR
(Dicle Üniversitesi, Tıp Fakültesi, Çocuk Cerrahisi Anabilim Dalı, Diyarbakır, Türkiye)
Aziz Serkan ARSLAN
(Dicle Üniversitesi, Tıp Fakültesi, Çocuk Cerrahisi Anabilim Dalı, Diyarbakır, Türkiye)
Hikmet ZEYTUN
(Dicle Üniversitesi, Tıp Fakültesi, Çocuk Cerrahisi Anabilim Dalı, Diyarbakır, Türkiye)
Gülay AYDOĞDU
(Dicle Üniversitesi, Tıp Fakültesi, Patoloji Anabilim Dalı, Diyarbakır, Türkiye)
Aysın EKİNCİ
(Dicle Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Diyarbakır, Türkiye)
Yıl: 2021Cilt: 4Sayı: 1ISSN: 2618-6454 / 2618-6454Sayfa Aralığı: 47 - 57İngilizce

46 0
Effect of gallic acid on liver injury during obstructive cholestasis after bile duct ligation in rat
Aim: To investigate the hepatoprotective, anti-inflammatory and antioxidant effects of gallic acid (GA) against obstructive cholestasis (OC) -induced liver damage in rats. Methods: Thirty female Sprague-Dawley rats were divided into three groups. Group 1 (n=10) was the sham-operated group. In group 2 and group 3, hepatoduodenal ligament dissection was performed after laparotomy. Once the common bile duct was made apparent, it was ligated with 4/0 silk surgical suture and cut between both sutures. Group 2 (n=10) was the control group. Group 3 (n=10) was the GA group. GA (50 mg/kg) was administered by oral gavage daily for 10 days. At the end of the experiment on day 10, the rats were anesthetized. Fibrosis, inflammation, ductal proliferation and necrosis were evaluated histopathologically. Serum levels of AST, ALT, TBIL, DBIL, LDH and GGT levels were determined. In the serum and liver, TAS, TOS, MDA, TNF-α, IL-1, IL-6, and IL 10 levels were evaluated. Results: When group 2 and group 3 were compared histopathologically, fibrosis and inflammation were significantly lower in group 3. In group 3, all LFTs (except DBIL), liver and serum IL-6, IL-1, TOS, MDA, and TNF-α levels were significantly lower than group 2, whereas IL-10 and TAS values were increased. Conclusion: Findings of this research indicate that GA may be effective against OC-induced liver damage in a rat model. We presume that the beneficial effects of GA are closely associated with its antioxidant and anti-inflammatory activities. Therefore, we think that using GA can save us time before resorting to the surgical method.
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