EFFECTS OF THYMOQUINONE, ZEOLITE AND PLATELET RICH PLASMA ON THE HEALING OF ISCHEMIC COLONIC ANASTOMOSIS

Özden, Hüseyin; Aydın, Oktay; ALTUNKAYA, CANAN; PEKCICI, MEVLUT RECEP; Uzun, Hafize; PEHLIVANLI, FARUK; ŞAHİNER, İbrahim Tayfun; KARACA, GÖKHAN

EFFECTS OF THYMOQUINONE, ZEOLITE AND PLATELET RICH PLASMA ON THE HEALING OF ISCHEMIC COLONIC ANASTOMOSIS

Faruk PEHLİVANLI, (Kırıkkale Üniversitesi, Tıp Fakültesi, Genel Cerrahi Anabilim Dalı)

Gökhan KARACA, (Kırıkkale Üniversitesi, Tıp Fakültesi, Genel Cerrahi Anabilim Dalı)

Oktay AYDIN, (Kırıkkale Üniversitesi, Tıp Fakültesi, Genel Cerrahi Anabilim Dalı)

Canan ALTUNKAYA, (Kırıkkale Üniversitesi, Tıp Fakültesi, Patoloji Anabilim Dalı)

İbrahim Tayfun ŞAHİNER, (Hitit Üniversitesi, Tıp Fakültesi, Genel Cerrahi Anabilim Dalı)

Hüseyin ÖZDEN, (Ahi Evran Üniversitesi, Tıp Fakültesi, Genel Cerrahi Anabilim Dalı)

Hafize UZUN, (İstanbul Üniversitesi, Tıp Fakültesi, Biyokimya Bölümü)

Mevlüt Recep PEKCİCİ (Ankara Eğitim ve Araştırma Hastanesi)

Kırıkkale Üniversitesi Tıp Fakültesi Dergisi

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Yıl: 2019 Cilt: 21 Sayı: 1 Sayfa Aralığı: 65 - 72 Metin Dili: İngilizce İndeks Tarihi: 25-05-2021

EFFECTS OF THYMOQUINONE, ZEOLITE AND PLATELET RICH PLASMA ON THE HEALING OF ISCHEMIC COLONIC ANASTOMOSIS

Öz:

Objective: Anastomosis on ischemic intestines is a problematicissue in daily surgical routine. In this study, we investigated thepotential effects of thymoquinone, zeolite and platelet rich plasma.Material and Methods: Fifty rats were divided into five groups.In the sham group all rats underwent colonic anastomosis withoutany ischemic insult. Colonic anastomosis was performed aftermesenteric ischemia was induced by clamping the superiormesenteric artery in other study groups. In the control group, nomedication was given to the rats after ishemic anastomosis. InZeolite group and thymoquinone group, Zeolite and thymoquinonewere applied locally on the anastomosis. In the platelet richplasma group, platelet rich plasma was applied locally on theanastomosis. All the rats were sacrified on the 10th day. Blood andtissue samples were retrieved.Results: Hydroxyproline levels were not different between thegroups. Bursting pressure was higher in the thymoquinone groupthan the control group. Reepithelization was higher than thezeolite group in the sham and thymoquinone groups. TNF-α andIL 1β levels were significantly higher in the sham group than allthe other groups. TNF-α and IL 1β levels were also significantlyhigher in the thymoquinone, zeolite and platelet rich plasmagroups than the control group and higher in the thymoquinonegroup than the zeolite and platelet rich plasma groups.Inflammation and ischemic necrosis levels were not differentbetween the groups.Conclusion: Our data is suggesting that thymoquinone andplatelet rich plasma have positive effects on ischemic anastomosishealing, while thymoquinone has the best results.

Anahtar Kelime:

İskemik Kolon Anastomoz İyileşmesinde Timokinon, Zeolit ve Plateletten Zengin Plazmanın Etkinliği

Öz:

Amaç: Günlük cerrahi rutinde iskemik barsakların anastomozu önemli bir sorundur. Biz bu çalışmada timokinon, zeolit ve plateletten zengin plazmanın potansiyel etkilerini araştırdık. Gereç ve Yöntemler: Toplam elli rat beş gruba ayrıldı. Sham grubuna iskemi olmaksızın kolonik anastomoz yapıldı. Çalışma gruplarında superior mezenterik arterin klemplenmesiyle oluşturulan mezenterik iskemi sonrası kolon anastomozu yapıldı. Kontrol grubuna iskemik anastomoz sonrası ilaç verilmedi. Zeolit, timokinon ve plateletten zengin plazma grubunda ilaç lokal olarak uygulandı. 10. günde ratlar sakrifiye edildi, doku ve kan örnekleri alındı. Bulgular: Hidroksiprolin seviyelerinde gruplararası fark yoktu. Patlama basıncı timokinon grubunda kontrol grubuna göre daha yüksekti. Reepitelizasyon sham ve timokinon grubuna göre zeolit grubunda daha yüksekti. Sham grubunda diğer gruplara kıyasla TNF-α ve IL 1β düzeyleri anlamlı olarak yüksekti. Ayrıca TNF-α ve IL 1β düzeyleri timokinon, zeolit ve plateletten zengin plazma gruplarında kontrol grubuna göre anlamlı olarak yüksekti ve bu üçü arasında timokinon grubunda en yüksekti. İnflamasyon ve iskemik nekroz seviyeleri arasında gruplar arası anlamlı fark yoktu. Sonuç: Sonuçlarımız timokinon ve plateletten zengin plazma’nin iskemik anastomoz iyileşmesi üzerine olumlu etkileri olduğunu göstermiş ve timokinon ile en iyi sonuçlar elde edilmiştir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	PEHLIVANLI F, KARACA G, Aydın O, ALTUNKAYA C, ŞAHİNER İ, Özden H, Uzun H, PEKCICI M (2019). EFFECTS OF THYMOQUINONE, ZEOLITE AND PLATELET RICH PLASMA ON THE HEALING OF ISCHEMIC COLONIC ANASTOMOSIS. , 65 - 72.
Chicago	PEHLIVANLI FARUK,KARACA GÖKHAN,Aydın Oktay,ALTUNKAYA CANAN,ŞAHİNER İbrahim Tayfun,Özden Hüseyin,Uzun Hafize,PEKCICI MEVLUT RECEP EFFECTS OF THYMOQUINONE, ZEOLITE AND PLATELET RICH PLASMA ON THE HEALING OF ISCHEMIC COLONIC ANASTOMOSIS. (2019): 65 - 72.
MLA	PEHLIVANLI FARUK,KARACA GÖKHAN,Aydın Oktay,ALTUNKAYA CANAN,ŞAHİNER İbrahim Tayfun,Özden Hüseyin,Uzun Hafize,PEKCICI MEVLUT RECEP EFFECTS OF THYMOQUINONE, ZEOLITE AND PLATELET RICH PLASMA ON THE HEALING OF ISCHEMIC COLONIC ANASTOMOSIS. , 2019, ss.65 - 72.
AMA	PEHLIVANLI F,KARACA G,Aydın O,ALTUNKAYA C,ŞAHİNER İ,Özden H,Uzun H,PEKCICI M EFFECTS OF THYMOQUINONE, ZEOLITE AND PLATELET RICH PLASMA ON THE HEALING OF ISCHEMIC COLONIC ANASTOMOSIS. . 2019; 65 - 72.
Vancouver	PEHLIVANLI F,KARACA G,Aydın O,ALTUNKAYA C,ŞAHİNER İ,Özden H,Uzun H,PEKCICI M EFFECTS OF THYMOQUINONE, ZEOLITE AND PLATELET RICH PLASMA ON THE HEALING OF ISCHEMIC COLONIC ANASTOMOSIS. . 2019; 65 - 72.
IEEE	PEHLIVANLI F,KARACA G,Aydın O,ALTUNKAYA C,ŞAHİNER İ,Özden H,Uzun H,PEKCICI M "EFFECTS OF THYMOQUINONE, ZEOLITE AND PLATELET RICH PLASMA ON THE HEALING OF ISCHEMIC COLONIC ANASTOMOSIS." , ss.65 - 72, 2019.
ISNAD	PEHLIVANLI, FARUK vd. "EFFECTS OF THYMOQUINONE, ZEOLITE AND PLATELET RICH PLASMA ON THE HEALING OF ISCHEMIC COLONIC ANASTOMOSIS". (2019), 65-72.

APA	PEHLIVANLI F, KARACA G, Aydın O, ALTUNKAYA C, ŞAHİNER İ, Özden H, Uzun H, PEKCICI M (2019). EFFECTS OF THYMOQUINONE, ZEOLITE AND PLATELET RICH PLASMA ON THE HEALING OF ISCHEMIC COLONIC ANASTOMOSIS. Kırıkkale Üniversitesi Tıp Fakültesi Dergisi, 21(1), 65 - 72.
Chicago	PEHLIVANLI FARUK,KARACA GÖKHAN,Aydın Oktay,ALTUNKAYA CANAN,ŞAHİNER İbrahim Tayfun,Özden Hüseyin,Uzun Hafize,PEKCICI MEVLUT RECEP EFFECTS OF THYMOQUINONE, ZEOLITE AND PLATELET RICH PLASMA ON THE HEALING OF ISCHEMIC COLONIC ANASTOMOSIS. Kırıkkale Üniversitesi Tıp Fakültesi Dergisi 21, no.1 (2019): 65 - 72.
MLA	PEHLIVANLI FARUK,KARACA GÖKHAN,Aydın Oktay,ALTUNKAYA CANAN,ŞAHİNER İbrahim Tayfun,Özden Hüseyin,Uzun Hafize,PEKCICI MEVLUT RECEP EFFECTS OF THYMOQUINONE, ZEOLITE AND PLATELET RICH PLASMA ON THE HEALING OF ISCHEMIC COLONIC ANASTOMOSIS. Kırıkkale Üniversitesi Tıp Fakültesi Dergisi, vol.21, no.1, 2019, ss.65 - 72.
AMA	PEHLIVANLI F,KARACA G,Aydın O,ALTUNKAYA C,ŞAHİNER İ,Özden H,Uzun H,PEKCICI M EFFECTS OF THYMOQUINONE, ZEOLITE AND PLATELET RICH PLASMA ON THE HEALING OF ISCHEMIC COLONIC ANASTOMOSIS. Kırıkkale Üniversitesi Tıp Fakültesi Dergisi. 2019; 21(1): 65 - 72.
Vancouver	PEHLIVANLI F,KARACA G,Aydın O,ALTUNKAYA C,ŞAHİNER İ,Özden H,Uzun H,PEKCICI M EFFECTS OF THYMOQUINONE, ZEOLITE AND PLATELET RICH PLASMA ON THE HEALING OF ISCHEMIC COLONIC ANASTOMOSIS. Kırıkkale Üniversitesi Tıp Fakültesi Dergisi. 2019; 21(1): 65 - 72.
IEEE	PEHLIVANLI F,KARACA G,Aydın O,ALTUNKAYA C,ŞAHİNER İ,Özden H,Uzun H,PEKCICI M "EFFECTS OF THYMOQUINONE, ZEOLITE AND PLATELET RICH PLASMA ON THE HEALING OF ISCHEMIC COLONIC ANASTOMOSIS." Kırıkkale Üniversitesi Tıp Fakültesi Dergisi, 21, ss.65 - 72, 2019.
ISNAD	PEHLIVANLI, FARUK vd. "EFFECTS OF THYMOQUINONE, ZEOLITE AND PLATELET RICH PLASMA ON THE HEALING OF ISCHEMIC COLONIC ANASTOMOSIS". Kırıkkale Üniversitesi Tıp Fakültesi Dergisi 21/1 (2019), 65-72.