PROGESTERONE INCREASES THE ISCHEMIC DAMAGE IN MALE RATS WITH CEREBRAL ISCHEMIA REPERFUSION INJURY
Yıl: 2019 Cilt: 21 Sayı: 2 Sayfa Aralığı: 183 - 194 Metin Dili: İngilizce DOI: 10.24938/kutfd.531788 İndeks Tarihi: 26-05-2021
PROGESTERONE INCREASES THE ISCHEMIC DAMAGE IN MALE RATS WITH CEREBRAL ISCHEMIA REPERFUSION INJURY
Öz: Objective: In the current literature, there are few acceptedpharmacological treatment methods for acute ischemic stroke.This study was conducted to investigate the effects ofprogesterone on transient ischemia / reperfusion injury in malerats.Material and Methods: A total of 25 Wistar albino male andyoung rats were divided into 5 groups called Control group,acute stage groups (Sham-A and PRG-A), and chronic stagegroups (Sham-C and PRG-C), randomly and their internalcarotid arteries were compressed using temporary aneurysmclips for 30 minutes. At 4 hours after removal of the clips,progesterone was injected to the animals of the PRG-A andPRG-C group via intraperitoneal route. After sacrifice of allanimals, pyknotic and necrotic neuronal cells were counted inhippocampal cornu amnonis (CA)1, CA2, CA3 and parietalcortical regions, histopathologically. Tissue interleukin (IL)-6,IL-10, caspase-3, and hypoxia-inducible factor-1 (HIF1) geneexpression levels were evaluated using real time polymerasechain reaction assay.Results: Histopathological and biochemical findings revealedthat progesterone has no healing effects on ischaemic neuronaltissue damage in either acute or chronic period. Moreover,progesterone was found to significantly increase symptoms ofischaemia in both acute and chronic periods compared tohealthy control group and even compared to Sham groups whereI/R injury was applied and no experimental agent wasadministered.Conclusion: At the end of this study, it was thought thatprogesterone had no therapeutic effect on cerebral ischemia /reperfusion injury in male sex rats and it could lead to increase itfurther, unfortunately.
Anahtar Kelime: Progesteron Serebral İskemi Yapılan Erkek Ratlarda İskemik Hasarı Artırır
Öz: Amaç: Günümüzde akut iskemik inme tedavisine yönelik literatürde çok az sayıda kabul edilmiş etkili farmakolojik tedavi yöntemi bulunmaktadır. Bu deneysel çalışma progesteronun erkek ratlarda oluşturulan geçici iskemi/ reperfüzyon hasarı üzerine olan etkilerini araştırmak amacıyla yapıldı. Gereç ve Yöntemler: Kontrol grubu (n=5) dışında, 20 adet Wistar albino erkek sıçanı (Sham-A, Sham-C, PRG-A, PRGC) dört gruba dağıtıldı ve geçici anevrizma klipleri 30 dakika boyunca internal karotid artere uygulandı. Klipler alındıktan dört saat sonra, PRG-A ve PRG-C gruplarına intraperitoneal olarak progesteron uygulandı. Tüm hayvanlar sakrifiye edildikten sonra, hipokampal cornu ammnonis CA 1, CA2, CA3 ve parietal korteksteki piknotik ve nekrotik nöronal hücreler histopatolojik olarak sayıldı. Ek olarak, doku interlökin (IL)-6, IL-10, kaspaz-3, HIF1 gen ekspresyon seviyeleri ve real time polimeraz zincir reaksiyonu değerlendirildi. Bulgular: Progesteronun iskeminin hem akut hemde kronik döneminde iskemik nöronal doku hasarı üzerinde histopatolojik ve biyokimyasal veriler bakımından iyileştirici etkilerinin olmadığı görüldü. Hatta progesteronun iskemi bulgularını hem akut dönemde ve hem de kronik dönemde sağlıklı dokulara göre ve hatta sadece cerrahi girişim yapılıp hiçbir deneysel ajan verilmeyen deney gruplarına göre belirgin şekilde arttırdığı gözlendi. Sonuç: Bu çalışma sonunda progesteronun erkek cinsiyetteki ratlarda oluşturulan serebral iskemi/reperfüzyon hasarında tedavi edici etkinliğinin olmadığı ve bu hasarı daha da artırdığı düşünüldü.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- 1. Elijovich L, Chong JY. Current and future use of intravenous thrombolysis for acute ischemic stroke. Curr Atherosclerol Rep. 2010;12(5):316-21.
- 2. Ishrat T, Sayeed I, Atif F, Stein DG. Effects of progesterone administration on infarct volume and functional deficits following permanent focal cerebral ischemia in rats. Brain Res. 2009;1257:94- 101.
- 3. Espinosa-Garcia C, Aguilar-Hernandez A, Cervantes M, Moralí G. Effects of progesterone on neurite growth inhibitors in the hippocampus following global cerebral ischemia. Brain Res. 2014;1545:23-34.
- 4. Schumacher M, Robel P, Baulieu EE. Development and regeneration of the nervous system: a role for neurosteroids. Dev Neurosci. 1996;18(1-2):6-21.
- 5. Yousuf S, Atif F, Sayeed I, Tang H, Stein DG. Progesterone in transient ischemic stroke: a doseresponse study. Psychopharmacology (Berl). 2014;231(17):3313-23.
- 6. Alkayed NJ, Harukuni I, Kimes AS, London ED, Traystman RJ, Hurn PD. Gender-linked brain injury in experimental stroke. Stroke. 1998;29(1):159-66.
- 7. Murphy SJ, Traystman RJ, Hurn PD, Duckles SP. Progesterone exacerbates striatal stroke injury in progesterone-deficient female animals. Stroke. 2000;31(5):1173-8.
- 8. Coomber B, Gibson CL. Sustained levels of progesterone prior to the onset of cerebral ischemia are not beneficial to female mice. Brain Res. 2010;1361:124-32.
- 9. Wong R, Renton C, Gibson CL, Murphy SJ, Kendall DA, Bath PM. Progesterone treatment for experimental stroke: an individual animal metaanalysis. J Cereb Blood Flow Metab. 2013;33(9):1362-72.
- 10.Calvert JW, Cahill J, Yamaguchi-Okada M, Zhang JH. Oxygen treatment after experimental hypoxiaischemia in neonatal rats alters the expression of HIF-1alpha and its downstream target genes. J Appl Physiol. 2006;101(3):853-65.
- 11. Gredal H, Thomsen BB, Boza-Serrano A, Garosi L, Rusbridge C, Anthony D et al. Interleukin-6 is increased in plasma and cerebrospinal fluid of community-dwelling domestic dogs with acute ischaemic stroke. Neuroreport. 2017;28(3):134-140.
- 12. Li SJ, Liu W, Wang JL, Zhang Y, Zhao DJ, Wang TJ et al. The role of TNF-α, IL-6, IL-10, and GDNF in neuronal apoptosis in neonatal rat with hypoxicischemic encephalopathy. Eur Rev Med Pharmacol Sci. 2014;18(6):905-9.
- 13. Waje-Andreassen U, Kråkenes J, Ulvestad E, Thomassen L, Myhr KM, Aarseth J et al. IL-6: an early marker for outcome in acute ischemic stroke. Acta Neurol Scand. 2005;111(6):360-5.
- 14. Habib P, Dang J, Slowik A, Victor M, Beyer C. Hypoxia-induced gene expression of aquaporin-4, cyclooxygenase-2 and hypoxia-inducible factor 1α in rat cortical astroglia is inhibited by 17β-estradiol and progesterone. Neuroendocrinology. 2014;99(3- 4):156-67.
- 15. Mohamed RA, Agha AM, Nassar NN. SCH58261 the selective adenosine A (2A) receptor blocker modulates ischemia reperfusion injury following bilateral carotid occlusion: role of inflammatory mediators. Neurochem Res. 2012;37(3):538-47.
| APA | ÖĞDEN M, BAKAR B, ALTUNKAYA C, Dağlı A, Özdemir A, Ozveren M (2019). PROGESTERONE INCREASES THE ISCHEMIC DAMAGE IN MALE RATS WITH CEREBRAL ISCHEMIA REPERFUSION INJURY. , 183 - 194. 10.24938/kutfd.531788 |
| Chicago | ÖĞDEN MUSTAFA,BAKAR BULENT,ALTUNKAYA CANAN,Dağlı Ahmet Turan,Özdemir Alemiddin,Ozveren Mehmet Faik PROGESTERONE INCREASES THE ISCHEMIC DAMAGE IN MALE RATS WITH CEREBRAL ISCHEMIA REPERFUSION INJURY. (2019): 183 - 194. 10.24938/kutfd.531788 |
| MLA | ÖĞDEN MUSTAFA,BAKAR BULENT,ALTUNKAYA CANAN,Dağlı Ahmet Turan,Özdemir Alemiddin,Ozveren Mehmet Faik PROGESTERONE INCREASES THE ISCHEMIC DAMAGE IN MALE RATS WITH CEREBRAL ISCHEMIA REPERFUSION INJURY. , 2019, ss.183 - 194. 10.24938/kutfd.531788 |
| AMA | ÖĞDEN M,BAKAR B,ALTUNKAYA C,Dağlı A,Özdemir A,Ozveren M PROGESTERONE INCREASES THE ISCHEMIC DAMAGE IN MALE RATS WITH CEREBRAL ISCHEMIA REPERFUSION INJURY. . 2019; 183 - 194. 10.24938/kutfd.531788 |
| Vancouver | ÖĞDEN M,BAKAR B,ALTUNKAYA C,Dağlı A,Özdemir A,Ozveren M PROGESTERONE INCREASES THE ISCHEMIC DAMAGE IN MALE RATS WITH CEREBRAL ISCHEMIA REPERFUSION INJURY. . 2019; 183 - 194. 10.24938/kutfd.531788 |
| IEEE | ÖĞDEN M,BAKAR B,ALTUNKAYA C,Dağlı A,Özdemir A,Ozveren M "PROGESTERONE INCREASES THE ISCHEMIC DAMAGE IN MALE RATS WITH CEREBRAL ISCHEMIA REPERFUSION INJURY." , ss.183 - 194, 2019. 10.24938/kutfd.531788 |
| ISNAD | ÖĞDEN, MUSTAFA vd. "PROGESTERONE INCREASES THE ISCHEMIC DAMAGE IN MALE RATS WITH CEREBRAL ISCHEMIA REPERFUSION INJURY". (2019), 183-194. https://doi.org/10.24938/kutfd.531788 |
| APA | ÖĞDEN M, BAKAR B, ALTUNKAYA C, Dağlı A, Özdemir A, Ozveren M (2019). PROGESTERONE INCREASES THE ISCHEMIC DAMAGE IN MALE RATS WITH CEREBRAL ISCHEMIA REPERFUSION INJURY. Kırıkkale Üniversitesi Tıp Fakültesi Dergisi, 21(2), 183 - 194. 10.24938/kutfd.531788 |
| Chicago | ÖĞDEN MUSTAFA,BAKAR BULENT,ALTUNKAYA CANAN,Dağlı Ahmet Turan,Özdemir Alemiddin,Ozveren Mehmet Faik PROGESTERONE INCREASES THE ISCHEMIC DAMAGE IN MALE RATS WITH CEREBRAL ISCHEMIA REPERFUSION INJURY. Kırıkkale Üniversitesi Tıp Fakültesi Dergisi 21, no.2 (2019): 183 - 194. 10.24938/kutfd.531788 |
| MLA | ÖĞDEN MUSTAFA,BAKAR BULENT,ALTUNKAYA CANAN,Dağlı Ahmet Turan,Özdemir Alemiddin,Ozveren Mehmet Faik PROGESTERONE INCREASES THE ISCHEMIC DAMAGE IN MALE RATS WITH CEREBRAL ISCHEMIA REPERFUSION INJURY. Kırıkkale Üniversitesi Tıp Fakültesi Dergisi, vol.21, no.2, 2019, ss.183 - 194. 10.24938/kutfd.531788 |
| AMA | ÖĞDEN M,BAKAR B,ALTUNKAYA C,Dağlı A,Özdemir A,Ozveren M PROGESTERONE INCREASES THE ISCHEMIC DAMAGE IN MALE RATS WITH CEREBRAL ISCHEMIA REPERFUSION INJURY. Kırıkkale Üniversitesi Tıp Fakültesi Dergisi. 2019; 21(2): 183 - 194. 10.24938/kutfd.531788 |
| Vancouver | ÖĞDEN M,BAKAR B,ALTUNKAYA C,Dağlı A,Özdemir A,Ozveren M PROGESTERONE INCREASES THE ISCHEMIC DAMAGE IN MALE RATS WITH CEREBRAL ISCHEMIA REPERFUSION INJURY. Kırıkkale Üniversitesi Tıp Fakültesi Dergisi. 2019; 21(2): 183 - 194. 10.24938/kutfd.531788 |
| IEEE | ÖĞDEN M,BAKAR B,ALTUNKAYA C,Dağlı A,Özdemir A,Ozveren M "PROGESTERONE INCREASES THE ISCHEMIC DAMAGE IN MALE RATS WITH CEREBRAL ISCHEMIA REPERFUSION INJURY." Kırıkkale Üniversitesi Tıp Fakültesi Dergisi, 21, ss.183 - 194, 2019. 10.24938/kutfd.531788 |
| ISNAD | ÖĞDEN, MUSTAFA vd. "PROGESTERONE INCREASES THE ISCHEMIC DAMAGE IN MALE RATS WITH CEREBRAL ISCHEMIA REPERFUSION INJURY". Kırıkkale Üniversitesi Tıp Fakültesi Dergisi 21/2 (2019), 183-194. https://doi.org/10.24938/kutfd.531788 |
