Objective: In laboratories, the erythrocyte sedimentation rate (ESR) test may need to be repeated for various reasons. The aim of this study was to evaluate the repeatability performance of the ESR test by comparing the ESR values measured after different mixing procedures following the first measurement in sedimentation tubes. Material and Methods: The sedimentation tubes of 37 samples, which were brought to our clinical biochemistry laboratory for analysis on the same day, were first inverted very lightly and only once after the first measurement, and the second reading was performed. Then, the third reading was performed by inverting the tubes thoroughly and repeatedly. Results: While the ICC (intraclass correlation coefficient) value between the first and second measurements was 0.868, the ICC value between the first and third measurements was 0.927. In the third measurement, there was a better agreement with the first measurement compared to the second measurement. Conclusion: In cases where the ESR assay needs to be repeated, sedimentation tubes should be inverted thoroughly and repeatedly. However, despite this application, the compatibility between measurements may not be at the desired level.