Comparison of the effects of two pre-synchronization protocols (G6G and PG-3-G) on some reproductive performance parameters in Holstein cows
Yıl: 2020 Cilt: 36 Sayı: 4 Sayfa Aralığı: 248 - 254 Metin Dili: İngilizce DOI: 10.15312/EurasianJVetSci.2020.305 İndeks Tarihi: 20-05-2021
Comparison of the effects of two pre-synchronization protocols (G6G and PG-3-G) on some reproductive performance parameters in Holstein cows
Öz: Aim: The present study was aimed to determine effects of G6G and PG-3-Gpresynchronization protocols on some reproductive parameters in Holsteincows between postpartum 28-40 days.Materials and Methods: Cows in the G6G group (n=35) were treated withPGF2α on day (d) 0, GnRH on days 2 and 8, and PGF2α on day 15. The injectionof last GnRH was applied 56 hours after the second PGF2α injection and thentimed artifical insemination (TAI) was performed 16-18 hours later. Cows inthe PG-3-G group (n=35) were treated with PGF2α on day 0, GnRH on day 3and 10, and PGF2α on day 17. The injection of last GnRH and TAI were appliedas they were done for G6G group.Results: There were no statistical differences between the G6G and PG-3-Ggroups in terms of submission rate (100% and 100%), the first-service pregnancy per artificial insemination (P/AI) (42.9% and 51.4%), calving to firstinsemination interval (53.5 and 54.0 d), calving to conception interval (54.1and 53.7 d), calving interval (334.1 and 333.7 d) were determined. When thedata analyzed according to the protocol at the beginning of cows to be "cyclic"and "noncyclic", P/AI were found to be 64.7% and 41.5%, respectively. Thedifference between the groups was tended to be significant (p=0.098).Conclusion: Start of presynchronization protocols in the early postpartum period, provides 100% SR after the voluntary waiting period. It is clearly shownthat the presynchronization protocols reduces the calving interval less than400 days. Time and labor cost could be saved as there would be no needs foroestrus detection and appropriate P/AI could also be achieved in non-cycliccows.
Anahtar Kelime: Holstein ineklerde iki ön senkronizasyon protokolünün (G6G ve PG-3-G) bazı reprodüktif performans parametreleri üzerindeki etkilerinin karşılaştırılması
Öz: Amaç: Bu çalışmada postpartum 28-40 günler arasında uygulanan G6G ve PG3-G ön senkronizasyon protokollerinin Holstein ineklerde bazı üreme parametreleri üzerindeki etkilerinin belirlenmesi amaçlanmıştır. Gereç ve Yöntem: G6G grubundaki ineklere (n=35) 0. gün PGF2α, 2. gün GnRH, 8. gün GnRH, 15. gün PGF2α, ikinci PGF2α uygulamasından sonraki 56. saatte GnRH uygulaması yapıldı ve bunu takiben 16-18. saatte zamanlı suni tohumlama işlemi uygulandı. PG-3-G grubundaki ineklere (n=35) ise 0. gün PGF2α, 3. gün GnRH, 10. gün GnRH, 17. gün PGF2α, ikinci PGF2α uygulamasından sonraki 56. saatte GnRH uygulaması yapıldı ve bunu takiben 16-18. saatte zamanlı suni tohumlama işlemi uygulandı. Bulgular: G6G ve PG-3-G gruplarında arz oranı (%100 ve %100), ilk tohumlamada gebe kalma oranları (%42.9 ve %51.4), doğum ilk tohumlama aralığı (53.5 ve 54.0 gün), doğum gebe kalma aralığı (54.1 ve 53.7 gün), buzağılama aralığı (334.1 ve 333.7 gün) parametrelerinde gruplar arasında istatistiksel olarak farklılık bulunmadı. Protokol başlangıcında inekler siklik ve nonsiklik olmalarına göre değerlendirildiğinde ilk tohumlamada gebe kalma oranları nonsiklik ineklerde ve siklik ineklerde sırasıyla %64,7 ve %41,5 olarak tespit edildi. Yapılan istatistiksel analizde gruplar arasındaki farkın önemli olma eğiliminde olduğu görüldü (p=0,098). Öneri: Sonuç olarak postpartum erken dönemde başlatılan ön senkronizasyon protokolleri ile gönüllü bekleme süresini takip eden erken dönemde %100 arz oranı sağlanmıştır. Ön senkronizasyon protokolleri buzağılama aralığını 400 günden aşağı çekerek etkisini bariz şekilde ortaya koymuştur. Östrüs takibine gerek duyulmadan zaman ve iş gücünden tasarruf edilebileceği, nonsiklik ineklerde de uygun gebelik oranına ulaşılabileceği kanısına varılmıştır.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- Ahmed N, Doley S, Vanlalhriatpuia, 2017. Inclusion of ovulation synchronization strategies for augmentation of fertility in post-partum anestrus crossbred cows. IJCS, 5(4), 25-6.
- Aköz M, Aydın İ, Dinç DA, 2008. Efficacy of the presynchovsynch program on some reproductive parameters in postpartum dairy cows. Acta Veterinaria Beograd, 58, 477- 86.
- Astis A, Fargas O, 2013. Pregnancy per AI differences between primiparous and multiparous high-yield dairy cows after using double ovsynch or G6G synchronization protocols. Theriogenology, 7, 1065-70.
- Bello NM, Steibel JP, Pursley JR, 2006. Optimizing ovulation to first GnRH improved outcomes to each hormonal injection of ovsynch in lactating dairy cows. J Dairy Sci, 89, 3413-24.
- Bisinotto RS, Castro LO, Pansani MB, Narciso CD, et al., 2015. Progesterone supplementation to lactating dairy cows without a corpus luteum at initiation of the Ovsynch protocol. J Dairy Sci, 98, 2515–28.
- Borchardt S, Haimerl P, Pohl A, Heuwieser W, 2017. Evaluation of prostaglandin F2α versus prostaglandin F2α plus gonadotropin-releasing hormone as Presynch methods preceding an Ovsynch in lactating dairy cows: A metaanalysis. J Dairy Sci, 100(5), 4065–77.
- Cafre 2015. http://www.dardni.gov.uk/challengenote1csubmissionrates1.pdf, Accessed at: 08.10.2014.
- Chebel lRC, Santos JEP, Cerri RLA, Rutigliano HM, et al., 2006. Reproduction in dairy cows following progesterone insert presynchronization and resynchronization protocols. J Dairy Sci, 89, 4205-19.
- Dinç DA, 2013. Üremeye ilişkin reprodüktif (performans) parametreleri-II. Tüsedad, 21, 26-7.
- Dinç DA, Kutlu M, 2015. Reproductive performance parameters in dairy cows. Turkiye Klinikleri J Vet Sci Obstet Gynecol-Special Topics, 1(1), 17-31.
- Dirandeh E, Rezaei Roodbari A, Gholizadeh M, Deldar H, et al., 2015. Administration of prostaglandin F2α 14 d before initiating a G6G or a G7G timed artificial insemination protocol increased circulating progesterone prior to artificial insemination and reduced pregnancy loss in multiparous Holstein cows. J Dairy Sci, 98, 5414–21.
- Edmonson AJ, Lean IJ, Weaver LD, Farver T, et al., 1989. A body condition scoring chart for holstein dairy cows. J Dairy Sci, 72, 68-78.
- Gümen A, Guenther JN, Wiltbank MC, 2003. Follicular size and response to ovsynch versus detection of estrus in anovular and ovular lactating dairy cows. J Dairy Sci, 86, 3184–94.
- Heidari F, Dirandeh E, Ansari Pirsaraei Z, Colazo MG, 2017. Modifications of the G6G timed-AI protocol improved pregnancy per AI and reduced pregnancy loss in lactating dairy cows. Animal, 11(11), 2002-9.
- Herlihy MM, Giordano JO, Souza AH, Ayres H, et al., 2012. Presynchronization with double-ovsynch improves fertility at first postpartum artificial insemination in lactating dairy cows. J Dairy Sci, 95, 7003-14.
- Hudson C, Kerby M, Statham J, Wapenaar W, 2012. Managing herd reproduction, In: Dairy Herd Health, Ed; Green M, First edition, CABI, Oxfordshire, p: 86-110.
- Kutlu M, Dinç DA, 2015. Oestrus detection in dairy cows, submission rate and ways to increase it. Turkiye Klinikleri J Vet Sci Obstet Gynecol-Special Topics, 1(1), 32-41.
- Lopez H, Caraviello DZ, Satter LD, Fricke PM, et al., 2005. Relationship between level of milk production and multiple ovulations in lactating dairy cows. J Dairy Sci, 88, 2783-93.
- Mawhinney I, Biggadike H, 1998. A field study of the intercept planned breeding routine for dairy cows involving GnRH and PGF2alpha. Cattle Practice, 138, 515–21.
- Moreira F, De La Sota RL, Diaz T, Thatcher WW, 2000. Effect of day of the estrous cycle at the initiation of a timed artificial insemination protocol on reproductive responses in dairy heifers. J Anim Sci, 78, 1568-76.
- National Research Council, 2001. Dry Matter Intake, In: Nutrient requirements of dairy cattle, Ed; National Research Council, Seventh edition, National Academy Press, Washington DC, p: 1-363.
- Navanukraw C, Redmer DA, Reynolds LP, Kirsch JD, et al., 2004. A modified presynchronization protocol improves fertility to timed artificial insemination in lactating dairy cows. J Dairy Sci, 87, 1551-7.
- Opsomer G, Gröhn YT, Hertl J, Coryn M, et al., 2000. Risk factors for post partum ovarian dysfunction in high producing dairy cows in Belgium: a field study Theriogenology, 53, 841-57.
- Oztürk OA, Cirit U, Baran A, Ak K, 2010. Is Doublesynch protocol a new alternative for timed artificial insemination in anestrous dairy cows. Theriogenology, 73, 568-76.
- Peters MW, Pursley JR, 2002. Fertility of lactating dairy cows treated wiht ovsynch after presynchronization injections of PGF2α and GnRH. J Dairy Sci, 85, 2403– 6.
- Pulley SL, Keisler DH, Stevenson JS, 2015. Concentrations of luteinizing hormone and ovulatory responses in dairy cows before timed artificial insemination. J Dairy Sci, 98(9), 6188-201.
- Pursley JR, Martins JP, 2011. Enhancing fertility of lactating dairy cows. Michigan Dairy Review, 16(2), 1-3.
- Pursley JR 2015. https://www.youtube.com/ watch?v=Fw04wtPz1RU, Accessed at: 01.06.2015.
- Ribeiro ES, Cerri RLA, Bisinotto RS, Lima FS, et al., 2011. Reproductive performance of grazing dairy cows following presynchronization and resynchronization protocols. J Dairy Sci, 94, 4984–96.
- Ribeiro ES, Bisinotto RS, Favoreto MG, Martins LT, et al., 2012a. Fertility in dairy cows following presynchronization and administering twice the luteolytic dose of prostaglandin F2α as one or two injections in the 5-day timed artificial insemination protocol. Theriogenology, 78, 273–84.
- Ribeiro ES, Monteiro APA, Lima FS, Ayres H, et al., 2012b. Effects of presynchronization and length of proestrus on fertility of grazing dairy cows subjected to a 5-day timed artificial insemination protocol. J Dairy Sci, 95, 2513–22.
- Santos JEP, Thatcher WW, Chebel RC, Cerri RLA, et al., 2004. The effect of embryonic death rates in cattle on the efficacy of estrus synchronization programs. Anim Reprod Sci 82(83), 513–35.
- Santos JEP, Rutigliano HM, SaFilho MF, 2009. Risk factors for resumption of postpartum estrous cycle and embryonic survival in lactating dairy cows. Anim Reprod Sci, 110, 207–21.
- SAS, 2000. Statistics, Version 8.0 Edition, SAS Inst Inc, Cary, NC.
- Sonat A, Tekin K, Akçay E, 2014. Effect of β-carotene injection on fertility in cows synchronised with G6G/Ovsynch protocol. Vet Hekim Der Derg, 85(1), 17-23.
- Souza AH, Ayres H, Ferreira RM, Wiltbank MC, 2008. A new presynchronization system (double-ovsynch) increases fertility at first postpartum timed AI lactating dairy cows. Theriogenology, 70, 208-15.
- Stevenson JS, Pulley SL, 2012. Pregnancy per artificial insemination after presynchronizing estrous cycles with the presynch-10 protocol or prostaglandin F2α injection followed by gonadotropin-releasing hormone before ovsynch-56 in 4 dairy herds of lactating dairy cows. J Dairy Sci, 95, 6513–22.
- Stevenson JS, Pulley SL, Mellieon JHI, 2012. Prostaglandin F2α and gonadotropin-releasing hormone GnRH administration improve progesterone status, luteal number, and proportion of ovular and anovular dairy cows with corpora lutea before a timed AI program. J Dairy Sci, 95, 1831–44.
- Stevenson JS 2014. http://www.hoards.com/DE/presynch, Accessed at: 08.10.2014.
- Stevenson JS, 2016. Synchronization and artificial insemination strategies in dairy herds. Vet Clin North Am Food Anim Pract, 32, 349-64.
- Stevenson JS, Sauls JA, Mendonça LGD, Voelz BE, 2018. Dose frequency of prostaglandin F2α administration to dairy cows exposed to presynchronization and either 5- or 7-day ovsynch program durations: ovulatory and luteolytic risks. J Dairy Sci, 101(10), 9575–90.
- Wiltbank MC, Gümen A, Sartori R, 2002. Physiological classification of anovulatory conditions in cattle. Theriogenology, 57, 21-52.
- Wiltbank MC, Pursley JR, 2014. The cow as an induced ovulator: timed AI after synchronization of ovulation. Theriogenology, 81, 170–85.
- Yılmaz C, Yılmaz O, Uçar M, 2011. Effect of PGF2α and GnRH injections applied before ovsynch on pregnancy rates in cows and heifers. Kafkas Univ Vet Fak Derg, 17(4), 641-4.
- Yousuf MR, Martins JP, Ahmad N, Nobis K, et al., 2016. Presynchronization of lactating dairy cows with PGF2a and GnRH simultaneously, 7 days before Ovsynch have similar outcomes compared to G6G. Theriogenology, 86, 1607–14.
| APA | kutlu m, Dinç D (2020). Comparison of the effects of two pre-synchronization protocols (G6G and PG-3-G) on some reproductive performance parameters in Holstein cows. , 248 - 254. 10.15312/EurasianJVetSci.2020.305 |
| Chicago | kutlu metehan,Dinç Dursun Ali Comparison of the effects of two pre-synchronization protocols (G6G and PG-3-G) on some reproductive performance parameters in Holstein cows. (2020): 248 - 254. 10.15312/EurasianJVetSci.2020.305 |
| MLA | kutlu metehan,Dinç Dursun Ali Comparison of the effects of two pre-synchronization protocols (G6G and PG-3-G) on some reproductive performance parameters in Holstein cows. , 2020, ss.248 - 254. 10.15312/EurasianJVetSci.2020.305 |
| AMA | kutlu m,Dinç D Comparison of the effects of two pre-synchronization protocols (G6G and PG-3-G) on some reproductive performance parameters in Holstein cows. . 2020; 248 - 254. 10.15312/EurasianJVetSci.2020.305 |
| Vancouver | kutlu m,Dinç D Comparison of the effects of two pre-synchronization protocols (G6G and PG-3-G) on some reproductive performance parameters in Holstein cows. . 2020; 248 - 254. 10.15312/EurasianJVetSci.2020.305 |
| IEEE | kutlu m,Dinç D "Comparison of the effects of two pre-synchronization protocols (G6G and PG-3-G) on some reproductive performance parameters in Holstein cows." , ss.248 - 254, 2020. 10.15312/EurasianJVetSci.2020.305 |
| ISNAD | kutlu, metehan - Dinç, Dursun Ali. "Comparison of the effects of two pre-synchronization protocols (G6G and PG-3-G) on some reproductive performance parameters in Holstein cows". (2020), 248-254. https://doi.org/10.15312/EurasianJVetSci.2020.305 |
| APA | kutlu m, Dinç D (2020). Comparison of the effects of two pre-synchronization protocols (G6G and PG-3-G) on some reproductive performance parameters in Holstein cows. Eurasian Journal of Veterinary Sciences, 36(4), 248 - 254. 10.15312/EurasianJVetSci.2020.305 |
| Chicago | kutlu metehan,Dinç Dursun Ali Comparison of the effects of two pre-synchronization protocols (G6G and PG-3-G) on some reproductive performance parameters in Holstein cows. Eurasian Journal of Veterinary Sciences 36, no.4 (2020): 248 - 254. 10.15312/EurasianJVetSci.2020.305 |
| MLA | kutlu metehan,Dinç Dursun Ali Comparison of the effects of two pre-synchronization protocols (G6G and PG-3-G) on some reproductive performance parameters in Holstein cows. Eurasian Journal of Veterinary Sciences, vol.36, no.4, 2020, ss.248 - 254. 10.15312/EurasianJVetSci.2020.305 |
| AMA | kutlu m,Dinç D Comparison of the effects of two pre-synchronization protocols (G6G and PG-3-G) on some reproductive performance parameters in Holstein cows. Eurasian Journal of Veterinary Sciences. 2020; 36(4): 248 - 254. 10.15312/EurasianJVetSci.2020.305 |
| Vancouver | kutlu m,Dinç D Comparison of the effects of two pre-synchronization protocols (G6G and PG-3-G) on some reproductive performance parameters in Holstein cows. Eurasian Journal of Veterinary Sciences. 2020; 36(4): 248 - 254. 10.15312/EurasianJVetSci.2020.305 |
| IEEE | kutlu m,Dinç D "Comparison of the effects of two pre-synchronization protocols (G6G and PG-3-G) on some reproductive performance parameters in Holstein cows." Eurasian Journal of Veterinary Sciences, 36, ss.248 - 254, 2020. 10.15312/EurasianJVetSci.2020.305 |
| ISNAD | kutlu, metehan - Dinç, Dursun Ali. "Comparison of the effects of two pre-synchronization protocols (G6G and PG-3-G) on some reproductive performance parameters in Holstein cows". Eurasian Journal of Veterinary Sciences 36/4 (2020), 248-254. https://doi.org/10.15312/EurasianJVetSci.2020.305 |
