The effect of interferon alpha administration on cytokine levels in sheep
Yıl: 2020 Cilt: 36 Sayı: 4 Sayfa Aralığı: 324 - 330 Metin Dili: İngilizce DOI: 10.15312/EurasianJVetSci.2020.315 İndeks Tarihi: 20-05-2021
The effect of interferon alpha administration on cytokine levels in sheep
Öz: Aim: The primary aim of this study was to determine the effect ofparenteral recombinant human interferon (rHuIFN)-α2a administration on serum tumor necrosis factor (TNF)-α, interleukin (IL)-6and IL-10 levels in healthy sheep. In addition, the main physiologicalparameters (rectal temperature, pulse rate, respiratory rate), hemogram and blood gas parameters were determined, as well.Materials and Methods: In this study, 9.000.000 IU rHuIFN-α2awas administered subcutaneously in 10 Merinos sheep. Blood samples were taken before (0 hours) and after at 4, 8, 12, 24, 48, 72, 96and 120 hours. Rectal temperature, pulse and respiratory rate werealso determined at the same sampling times. Sheep-specific TNF-α,IL-6 and IL-10 levels were determined from serum samples by theELISA reader. Hemogram and blood gas parameters were measuredby complete blood cell counter and blood gas analyzer, respectively.Results: After rHuIFN-α2a treatment, TNF-α and IL-10 concentrations reached peak levels (p<0.05) at the 96 hours, whereas the IL-6level did not change in a statistically significant (p>0.05). On the other hand, a temporary increase in rectal temperature and pO2 levels(p<0.05) were determined, while decreased potassium and ionizedcalcium levels (p<0.05) were measured. Statistically significantly(p<0.05) fluctuations were determined in pulse rate, white bloodcell counts, pH, base(ecf) and sodium values.Conclusion: It may be stated that administration of rHuIFN-α2ashowed immunological effects in sheep, as it is generally accepted assafe and can be considered for use in treatment.
Anahtar Kelime: Koyunlara interferon alfa uygulamasının sitokinler üzerine etkisi
Öz: Amaç: Bu çalışmanın temel amacı, sağlıklı koyunlara parenteral rekombinant insan interferon (rHuIFN)-α2a uygulamasının serum tümör nekroz faktör (TNF)-α, interlöykin (IL)-6 ve IL-10 seviyeleri üzerindeki etkisini belirlemektir. Ayrıca sağlıklı koyunlarda temel fizyolojik parametreler (vücut ısısı, nabız, solunum hızı), hemogram ve kan gazı parametrelerine etkisini tespit etmektir. Gereç ve Yöntem: Bu çalışmada 10 Merinos koyuna 9.000.000 IU rHuIFN-α2a deri altı yolla uygulandı. Kan örnekleri uygulamadan önce (0 saat) ve sonra 4, 8, 12, 24, 48, 72, 96 ve 120 saatlerde alındı. Aynı örnekleme zamanlarında vücut ısısı, nabız ve solunum hızı da belirlendi. Serum örneklerinden koyun spesifik TNF-α, IL-6 ve IL-10 seviyeleri ELISA okuyucu ile ölçüldü. Hemogram ve kan gazı parametreleri sırasıyla tam kan hücresi sayım cihazı ve kan gazı analiz cihazında ölçüldü. Bulgular: rHuIFN-α2a uygulaması sonrasında TNF-α ve IL-10 konsantrasyonları 96. saatte pik düzeye (p<0.05) ulaşırken, IL-6 konsantrasyonunda istatistiksel olarak anlamlı değişim belirlenmedi (p>0.05). Ayrıca vücut ısısı ve pO2 düzeylerinde geçici artışlar (p<0.05) belirlenirken, potasyum ve iyonize kalsiyum seviyelerinde düşmeler (p<0.05) belirlendi. Nabız, akyuvar sayımı, pH, base(ecf) ve sodyum değerlerinde ise istatistiki dalgalanmalar (p<0.05) tespit edildi. Öneri: Koyunlara rHuIFN-α2a uygulamasının immünolojik etkiler gösterebileceği, genel olarak güvenli kabul edilebileceği ve tedavide kullanım için düşünülebileceği ifade edilebilir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Coşkun D, İDER M, ARSLAN M, CANBAR R, Yazar E (2020). The effect of interferon alpha administration on cytokine levels in sheep. , 324 - 330. 10.15312/EurasianJVetSci.2020.315 |
| Chicago | Coşkun Devran,İDER Merve,ARSLAN Mustafa Sedat,CANBAR Rahmi,Yazar Enver The effect of interferon alpha administration on cytokine levels in sheep. (2020): 324 - 330. 10.15312/EurasianJVetSci.2020.315 |
| MLA | Coşkun Devran,İDER Merve,ARSLAN Mustafa Sedat,CANBAR Rahmi,Yazar Enver The effect of interferon alpha administration on cytokine levels in sheep. , 2020, ss.324 - 330. 10.15312/EurasianJVetSci.2020.315 |
| AMA | Coşkun D,İDER M,ARSLAN M,CANBAR R,Yazar E The effect of interferon alpha administration on cytokine levels in sheep. . 2020; 324 - 330. 10.15312/EurasianJVetSci.2020.315 |
| Vancouver | Coşkun D,İDER M,ARSLAN M,CANBAR R,Yazar E The effect of interferon alpha administration on cytokine levels in sheep. . 2020; 324 - 330. 10.15312/EurasianJVetSci.2020.315 |
| IEEE | Coşkun D,İDER M,ARSLAN M,CANBAR R,Yazar E "The effect of interferon alpha administration on cytokine levels in sheep." , ss.324 - 330, 2020. 10.15312/EurasianJVetSci.2020.315 |
| ISNAD | Coşkun, Devran vd. "The effect of interferon alpha administration on cytokine levels in sheep". (2020), 324-330. https://doi.org/10.15312/EurasianJVetSci.2020.315 |
| APA | Coşkun D, İDER M, ARSLAN M, CANBAR R, Yazar E (2020). The effect of interferon alpha administration on cytokine levels in sheep. Eurasian Journal of Veterinary Sciences, 36(4), 324 - 330. 10.15312/EurasianJVetSci.2020.315 |
| Chicago | Coşkun Devran,İDER Merve,ARSLAN Mustafa Sedat,CANBAR Rahmi,Yazar Enver The effect of interferon alpha administration on cytokine levels in sheep. Eurasian Journal of Veterinary Sciences 36, no.4 (2020): 324 - 330. 10.15312/EurasianJVetSci.2020.315 |
| MLA | Coşkun Devran,İDER Merve,ARSLAN Mustafa Sedat,CANBAR Rahmi,Yazar Enver The effect of interferon alpha administration on cytokine levels in sheep. Eurasian Journal of Veterinary Sciences, vol.36, no.4, 2020, ss.324 - 330. 10.15312/EurasianJVetSci.2020.315 |
| AMA | Coşkun D,İDER M,ARSLAN M,CANBAR R,Yazar E The effect of interferon alpha administration on cytokine levels in sheep. Eurasian Journal of Veterinary Sciences. 2020; 36(4): 324 - 330. 10.15312/EurasianJVetSci.2020.315 |
| Vancouver | Coşkun D,İDER M,ARSLAN M,CANBAR R,Yazar E The effect of interferon alpha administration on cytokine levels in sheep. Eurasian Journal of Veterinary Sciences. 2020; 36(4): 324 - 330. 10.15312/EurasianJVetSci.2020.315 |
| IEEE | Coşkun D,İDER M,ARSLAN M,CANBAR R,Yazar E "The effect of interferon alpha administration on cytokine levels in sheep." Eurasian Journal of Veterinary Sciences, 36, ss.324 - 330, 2020. 10.15312/EurasianJVetSci.2020.315 |
| ISNAD | Coşkun, Devran vd. "The effect of interferon alpha administration on cytokine levels in sheep". Eurasian Journal of Veterinary Sciences 36/4 (2020), 324-330. https://doi.org/10.15312/EurasianJVetSci.2020.315 |
