The research was carried out in a single group pretest-posttest semi-experimental type, in order to evaluate the impact of the training given to the auxiliary service personnel (ASP) on hygienic hand washing compliance. The research was conducted with 159 ASP in; surgical clinics, operating room, intensive care units (ICU) and clinics with invasive procedures. The data were collected using the Personal Information Form and the ASP Hand Hygiene Compliance Scale. Practical training on hygienic hand washing was given to the participants. Before and 3 months after the training, the hand hygiene compliance of YHP were evaluated. Descriptive, Kolmogorov-Smirnov, Shapiro-Wilk, Wilcoxon, Friedman, Kruskal Wallis and Mann-Whitney U tests were used in the analyzes of the data. About the ASP participating in the research, it was determined that; 52.8% were between the ages of 31-40, 73% were male, 56% were primary school graduates, and 58.5% had over 11 years of working experience. It was determined that 44% of the ASP worked in the surgical service, 22% in the ICU and 14.5% in the operating room. It was determined that there was no significant difference in the average scores of the Hand Hygiene Compliance Scale before (69,27±15,29; min:16, max:84) and after (70,36±13,83; min:21, max:84) the hygienic hand washing training of ASPs. It was determined that, the "After Contact with the Patient" scores of the participants working in the operating room increased (X2K-W =9,218, p=0,027) after the training, and the scores of the ASP’s who received the in-house training 1 month ago increased significantly in the "After Contact with the Patient" sub-dimension (Z=-2,029, p=0,042) and total score (Z=-1,97, p=0,049). It was concluded that; ASP's hand hygiene compliance was high, and when the results of the training on hand hygiene were evaluated three months later there was no significant difference in hand hygiene compliance.