Yıl: 2020 Cilt: 31 Sayı: 5 Sayfa Aralığı: 400 - 409 Metin Dili: İngilizce DOI: 10.5152/tjg.2020.19358 İndeks Tarihi: 19-06-2021

Human dental follicle mesenchymal stem cells alleviate T cell response in inflamed tissue of Crohn’s patients

Öz:
Background/Aims: Crohn’s disease (CD) is a chronic inflammatory condition characterized by various abnormalities that lead to overly aggressive T cell responses. Our in vitro experiments aimed to investigate the potential use of Dental Follicle Mesenchymal Stem Cells (DF-MSCs) to suppress the exaggerated immune response in inflamed and noninflamed tissue of CD.Materials and Methods: Dental follicle tissues were obtained from extracted third molar teeth of three healthy volunteers who had no abscess or inflammatory diseases. In total, 11 patients were included in the experiment who had been diagnosed with CD and did not receive steroid maintenance therapy for more than one month. Mononuclear cells were isolated from inflamed and noninflamed tissues of CD. Isolated cells were stimulated with anti-CD3/anti-CD28 monoclonal antibodies in the presence and absence of DF-MSCs and analyzed for lymphocytes proliferation capacity and viability, T lymphocyte subsets, CD4+IL22BP and CD4+CD25+Foxp3+ regulatory T cell (Treg) frequencies and cytokine levels. Results: A significant downregulation of lymphocyte proliferation and CD4+IL22BP T cell ratio were found in inflamed cultures with DF-MSCs (p<0.005). Additionally, the frequency of Tregs increased with DF-MSCs (p<0.05). Proinflammatory cytokine levels (Tumor Necro-sis Factor (TNF)-α and IL-6) were decreased (p<0.05), and IL-10 levels were increased (p<0.05) in the supernatant of inflamed cultures.Conclusion: DF-MSCs reduced the inflammatory immune response, induced Tregs, and downregulated CD4+IL22BP T cell ratio in in-flamed samples of CD patients, which may be exploited for significant therapeutic use.
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  • 1. Abraham C, Cho JH. Inflammatory bowel disease. N Engl J Med 2009; 361: 2066-78. [CrossRef]
  • 2. Baumgart DC, Sandborn WJ. Crohn’s disease. The Lancet 2012; 380: 1590-605. [CrossRef]
  • 3. Belkaid Y and Hand T. Role of the Microbiota in Immunity and inflammation. Cell 2014; 157: 121-41. [CrossRef]
  • 4. Gálvez J. Role of Th17 Cells in the Pathogenesis of Human IBD. ISRN Inflamm 2014; 2014: 928461. [CrossRef]
  • 5. Sanchez-Munoz F, Dominguez-Lopez A, Yamamoto-Furusho JK. Role of cytokines in inflammatory bowel disease. World J Gastroenterol 2008; 14: 4280-8. [CrossRef]
  • 6. Li LJ, Gong C, Zhao MH, Feng BS. Role of interleukin-22 in inflammatory bowel disease. World J Gastroenterol 2014; 20: 18177-88. [CrossRef]
  • 7. Jones BC1, Logsdon NJ, Walter MR. Structure of IL-22 bound to its high-affinity IL-22R1 chain. Structure 2008; 16: 1333-44. [CrossRef]
  • 8. Pelczar P, Witkowski M, Perez LG. A pathogenic role for T cell-derived IL-22BP in inflammatory bowel disease. Science 2016: 354: 358-62. [CrossRef]
  • 9. Sciaudone G, Di Stazio C, Limongelli P, et al. Treatment of complex perianal fistulas in Crohn’s disease: infliximab, surgery or combined approach. Can J Surg 2010; 53: 299.
  • 10. Corica D, Romano C. Biological Therapy in Pediatric Inflammatory Bowel Disease: A Systematic Review. J Clin Gastroenterol 2017; 51: 100-10. [CrossRef]
  • 11. Lim WC, Wang Y, MacDonald JK, et al. Aminosalicylates for induction of remission or response in Crohn’s disease. Cochrane Database Syst Rev 2016; 7: CD008870. [CrossRef]
  • 12. Ullah I, Subbarao RB, Rho GJ. Human mesenchymal stem cells - current trends and future prospective. Biosci Rep 2015; 35: e00191. [CrossRef]
  • 13. Barry FP, Murphy JM. Mesenchymal stem cells: clinical applications and biological characterization. Int J Biochem Cell Biol 2004; 36: 568-84. [CrossRef]
  • 14. Yildırım S, Zibandeh N, Genc D, et al. The Comparison of the Immunologic Properties of Stem Cells Isolated from Human Exfoliated Deciduous Teeth, Dental Pulp, and Dental Follicles. Stem Cells Int 2016; 4682875. [CrossRef]
  • 15. Chen X, Yang B, Tian J, et al. Dental Follicle Stem Cells Ameliorate Lipopolysaccharide-Induced Inflammation by Secreting TGF-β3 and TSP-1 to Elicit Macrophage M2 Polarization. Cell Physiol Biochem 2018; 51: 2290-308. [CrossRef]
  • 16. Kim HS, Shin TH, Lee BC, et al. Human umbilical cord blood mesenchymal stem cells reduce colitis in mice by activating NOD2 signaling to COX2. Gastroenterology 2013; 145: 1392-403. [CrossRef]
  • 17. Trapecar M, Khan S, Roan NR, et al. An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies. AIDS Res Hum Retroviruses 2017; 33: 31-9. [CrossRef]
  • 18. Squillaro T, Peluso G, Galderisi U. Clinical Trials with Mesenchymal Stem Cells: An Update. Cell Transplant 2016; 25: 829-48. [CrossRef]
  • 19. Li J, Ueno A, Fort Gasia M, et al. Profiles of Lamina Propria T Helper Cell Subsets Discriminate Between Ulcerative Colitis and Crohn’s Disease. Inflamm Bowel Dis 2016; 22: 1779-92. [CrossRef]
  • 20. De Miguel MP, Fuentes-Julián S, Blázquez-Martínez A, et al. Immunosuppressive properties of mesenchymal stem cells: advances and applications. Curr Mol Med 2012; 12: 574-91. [CrossRef]
  • 21. Flores AI, Gómez-Gómez GJ, Masedo-González Á, et al. Stem cell therapy in inflammatory bowel disease: A promising therapeutic strategy? World J Stem Cells 2015; 7: 343-51. [CrossRef]
  • 22. Ansari S, Seagroves JT, Chen C, et al. Dental and orofacial mesenchymal stem cells in craniofacial regeneration: The prosthodontist’s point of view. J Prosthet Dent 2017; 118: 455-61. [CrossRef]
  • 23. Genç D, Zibandeh N, Nain E, et al. Dental follicle mesenchymal stem cells down-regulate Th2-mediated immune response in asthmatic patients mononuclear cells. Clin Exp Allergy 2018; 48: 663-78. [CrossRef]
  • 24. Shale M, Schiering C, Powrie F. CD4(+) T-cell subsets in intestinal inflammation. Immunol Rev 2013; 252: 164-82. [CrossRef]
  • 25. Meresse B, Cerf-Bensussan N. Innate T cell responses in human gut. Semin Immunol 2009; 21:121-9. [CrossRef]
  • 26. Karlsson F, Martinez NE, Gray L, et al. Therapeutic evaluation of ex vivo-generated versus natural regulatory T-cells in a mouse model of chronic gut inflammation. Inflamm Bowel Dis 2013; 19: 2282-94. [CrossRef]
  • 27. Prevosto C, Zancolli M, Canevali P, et al. Generation of CD4+ or CD8+ regulatory T cells upon mesenchymal stem cell-lymphocyte interaction. Haematologica 2007; 92: 881-8. [CrossRef]
  • 28. Andoh A, Yagi Y, Shioya M, et al. Mucosal cytokine network in inflammatory bowel disease. World J Gastroenterol 2008; 14: 5154-61. [CrossRef]
  • 29. Andoh A, Zhang Z, Inatomi O, et al. Interleukin-22, a member of the IL-10 subfamily, induces inflammatory responses in colonic subepithelial myofibroblasts. Gastroenterology 2005; 129: 969-84 [CrossRef]
  • 30. Chen ML, Sundrud MS. Cytokine networks and T cell subsets in inflammatory bowel diseases. Inflamm Bowel Dis 2016; 22: 1157-67. [CrossRef]
  • 31. Ito H. Anti-interleukin-6 therapy for Crohn’s disease. Curr Pharm Des 2003; 9: 295-305. [CrossRef]
  • 32. Adegbola SO, Sahnan K, Warusavitarne J, et al. Anti-TNF Therapy in Crohn’s Disease. Int J Mol Sci 2018; 19: 2244. [CrossRef]
  • 33. Pelczar P, Witkowski M, Perez LG, et al. A pathogenic role for T cell-derived IL-22BP in inflammatory bowel disease. Science 2016; 354: 358-62. [CrossRef]
APA Zibandeh N, Genç D, DURAN Y, Banzragch M, sok s, Göker K, ATUG O, Akkoç T (2020). Human dental follicle mesenchymal stem cells alleviate T cell response in inflamed tissue of Crohn’s patients. , 400 - 409. 10.5152/tjg.2020.19358
Chicago Zibandeh Noushin,Genç Deniz,DURAN YAZGÜL,Banzragch Munkhtsetseg,sok sakin,Göker Kamil,ATUG OZLEN,Akkoç Tunç Human dental follicle mesenchymal stem cells alleviate T cell response in inflamed tissue of Crohn’s patients. (2020): 400 - 409. 10.5152/tjg.2020.19358
MLA Zibandeh Noushin,Genç Deniz,DURAN YAZGÜL,Banzragch Munkhtsetseg,sok sakin,Göker Kamil,ATUG OZLEN,Akkoç Tunç Human dental follicle mesenchymal stem cells alleviate T cell response in inflamed tissue of Crohn’s patients. , 2020, ss.400 - 409. 10.5152/tjg.2020.19358
AMA Zibandeh N,Genç D,DURAN Y,Banzragch M,sok s,Göker K,ATUG O,Akkoç T Human dental follicle mesenchymal stem cells alleviate T cell response in inflamed tissue of Crohn’s patients. . 2020; 400 - 409. 10.5152/tjg.2020.19358
Vancouver Zibandeh N,Genç D,DURAN Y,Banzragch M,sok s,Göker K,ATUG O,Akkoç T Human dental follicle mesenchymal stem cells alleviate T cell response in inflamed tissue of Crohn’s patients. . 2020; 400 - 409. 10.5152/tjg.2020.19358
IEEE Zibandeh N,Genç D,DURAN Y,Banzragch M,sok s,Göker K,ATUG O,Akkoç T "Human dental follicle mesenchymal stem cells alleviate T cell response in inflamed tissue of Crohn’s patients." , ss.400 - 409, 2020. 10.5152/tjg.2020.19358
ISNAD Zibandeh, Noushin vd. "Human dental follicle mesenchymal stem cells alleviate T cell response in inflamed tissue of Crohn’s patients". (2020), 400-409. https://doi.org/10.5152/tjg.2020.19358
APA Zibandeh N, Genç D, DURAN Y, Banzragch M, sok s, Göker K, ATUG O, Akkoç T (2020). Human dental follicle mesenchymal stem cells alleviate T cell response in inflamed tissue of Crohn’s patients. Turkish Journal of Gastroenterology, 31(5), 400 - 409. 10.5152/tjg.2020.19358
Chicago Zibandeh Noushin,Genç Deniz,DURAN YAZGÜL,Banzragch Munkhtsetseg,sok sakin,Göker Kamil,ATUG OZLEN,Akkoç Tunç Human dental follicle mesenchymal stem cells alleviate T cell response in inflamed tissue of Crohn’s patients. Turkish Journal of Gastroenterology 31, no.5 (2020): 400 - 409. 10.5152/tjg.2020.19358
MLA Zibandeh Noushin,Genç Deniz,DURAN YAZGÜL,Banzragch Munkhtsetseg,sok sakin,Göker Kamil,ATUG OZLEN,Akkoç Tunç Human dental follicle mesenchymal stem cells alleviate T cell response in inflamed tissue of Crohn’s patients. Turkish Journal of Gastroenterology, vol.31, no.5, 2020, ss.400 - 409. 10.5152/tjg.2020.19358
AMA Zibandeh N,Genç D,DURAN Y,Banzragch M,sok s,Göker K,ATUG O,Akkoç T Human dental follicle mesenchymal stem cells alleviate T cell response in inflamed tissue of Crohn’s patients. Turkish Journal of Gastroenterology. 2020; 31(5): 400 - 409. 10.5152/tjg.2020.19358
Vancouver Zibandeh N,Genç D,DURAN Y,Banzragch M,sok s,Göker K,ATUG O,Akkoç T Human dental follicle mesenchymal stem cells alleviate T cell response in inflamed tissue of Crohn’s patients. Turkish Journal of Gastroenterology. 2020; 31(5): 400 - 409. 10.5152/tjg.2020.19358
IEEE Zibandeh N,Genç D,DURAN Y,Banzragch M,sok s,Göker K,ATUG O,Akkoç T "Human dental follicle mesenchymal stem cells alleviate T cell response in inflamed tissue of Crohn’s patients." Turkish Journal of Gastroenterology, 31, ss.400 - 409, 2020. 10.5152/tjg.2020.19358
ISNAD Zibandeh, Noushin vd. "Human dental follicle mesenchymal stem cells alleviate T cell response in inflamed tissue of Crohn’s patients". Turkish Journal of Gastroenterology 31/5 (2020), 400-409. https://doi.org/10.5152/tjg.2020.19358