Objective: There is a growing debate on the existence of the G-spot. G-spot amplification by various surgical interventions has become mainstream foresthetic vaginal surgery despite a lack of conclusive proof of the G-spot. The aim of this study was to search for histologic evidence in regions of so-calledhyperintense focus (HF) (considered as the G-spot) using magnetic resonance imaging (MRI) mapping and biopsied tissues.Materials and Methods: Fifteen patients who had grade 2 or higher anterior compartment defects were enrolled in the study. All patients were subjectedto MRI. When a HF was seen, its localization, dimensions, and distances to adjacent structures were measured in images. Dissections in the anterior vaginalwall were performed in accordance with the measurements derived from MRI and tissue measuring 0.5x0.5 cm was biopsied from the determined HF.Results: An HF was determined in MRI of three (20%) patients. However, no significant neurovascular tissue density was observed histologically in any ofthe biopsy specimens obtained from the surgical dissections under the guidance of MRI mapping.Conclusion: Our findings denote that there is no G-spot in the anterior vaginal wall.