Effects of Carnosic and Gallic Acid on Ram Sperm Parameters and Seminal Plasma Homocysteine-Nesfatin Levels after Thawing
Yıl: 2020 Cilt: 13 Sayı: 2 Sayfa Aralığı: 104 - 107 Metin Dili: İngilizce DOI: 10.47027/duvetfd.753033 İndeks Tarihi: 22-06-2021
Effects of Carnosic and Gallic Acid on Ram Sperm Parameters and Seminal Plasma Homocysteine-Nesfatin Levels after Thawing
Öz: In the presented study, the protective role of carnosic and gallic acid on post-thaw ram sperm acrosome and membrane integrity wasexamined, homocysteine (Hcy) and nesfatin levels were determined.In study semen samples from 6 Merino rams were used. Each ejaculate, split into five equal aliquots was diluted with extenders including0.05 mM gallic acid (GA), 2 mM gallic acid, 0.05 mM carnosic acid (CA), 0.2 mM carnosic acid and no additive (control) at 37 °C cooled to5 °C then frozen at nitrogen vapor.Freeze-thawed ram semen viability was achieved in gallic acid 2mM (57.13± 2.38%) group and statistical difference was found with controlgroup (45.08±2.98%) (p<0.05). There was no statistical difference between the groups in HOS test analysis (p>0.05). The lowest level ofhomocysteine was obtained in the gallic acid groups (0.67±0.11 and 0.61±0.26 μmol/L) and was found statistically different with thecontrol group (1.36±0.9 μmol/L) (p<0.05). No significant difference was found in nesfatin levels among groups (p>0.05).GA supplementation in ram semen extender has been determined to protect viability and HOS test parameters also to decreasehomocysteine level.
Anahtar Kelime: Karnosik ve Gallik Asidin Çözdürme Sonrası Koç Sperm Parametreleri ve Seminal Plazma Homosistein-Nesfatin Düzeyleri Üzerindeki Etkileri
Öz: Sunulan çalışmada koç spermasının çözüm sonu akrozom ve membran bütünlüğü üzerine karnosik ve gallik asidin koruyucu rolü incelendi, homosistein ve nesfatin düzeyleri belirlendi. Çalışmada 6 Merinos koçundan sperma örnekleri kullanıldı. Beş eşit parçaya bölünen ejakülatlardan, 0.05 mM gallik asit (GA), 2 mM gallik asit, 0.05 mM karnosik asit (CA), 0.2 mM karnosik asit ve katkı maddesi içermeyen (kontrol) gruplar oluşturuldu. Sulandırma işlemi 37 °C'de yapıldı ve 5 °C de soğutulduktan sonra azot buharında donduruldu. Çözüm sonu koç sperması canlılığı gallik asit 2mM (%57.13±2.38) grubunda, kontrol grubundan (45.08 ±% 2.98) istatistiksel olarak farklı bulundu (p<0.05). HOS test parametreleri değerlendirmesinde gruplar arasında istatistiksel fark bulunmadı (p> 0.05). En düşük homosistein seviyesi gallik asit gruplarında (0.67±0.11 ve 0.61±0.26 μmol/L) elde edildi ve kontrol grubu (1.36 ± 0.9 μmol/L) ile istatistiksel olarak farklı bulundu (p <0.05). Nesfatin düzeylerinde gruplar arasında anlamlı fark bulunmadı (p> 0.05). Sperma sulandırıcısına GA ilavesinin koç spermasının çözüm sonu canlılığı ve HOS test değerleri üzerine koruyucu etkisi ile birlikte homosistein seviyesini düşürücü etkisi olduğu belirlendi. Nesfatin düzeyinin değerlendirilmesinde gruplar arasında anlamlı fark bulunmadı.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- 1. Andrabi SMH, Maxwell WMC. (2007). A review on Reproductive Biotechnologies for Conservation of Endangered Mammalian Species. Anim Reprod Sci. 99: 223-243.
- 2. Purdy PH. (2006). The Post-thaw Quality of Ram Sperm Held for 0 to 48 h at 5oC Prior to Cryopreservation. Anim Reprod Sci. 93: 114-123.
- 3. Matsuoka T, Imai H, Kohno H, et al. (2006). Effect of Bovine Serum Albumin in Trehalose in Semen Diluents for Improvement of Frozen-Thawed Ram Spermatozoa. J Reprod Dev. 52: 675- 683.
- 4. Rizzo A, Sciorsci RL. (2019). Role of Homocysteine Metabolism in Animal Reproduction: A Review. Res Vet Sci. 122: 29-35.
- 5. Faraci FM, Lentz SR. (2004). Hyperhomocysteinemia, Oxidative Stress, and Cerebral Vascular Dysfunction. Stroke. 35: 345–347.
- 6. Petras M, Tatarkova Z, Kovalska M, et al. (2014). Hyperhomocysteinemia as a Risk Factor for the Neuronal System Disorders. J Physiol Pharmacol. 65(1): 15-23.
- 7. Kim J, Yang H. (2012). Nesfatin-1 as a New Potent Regulator in Reproductive System. Dev Reprod. 16(4): 253.
- 8. Oh-I S, Shimizu H, Satoh T, et al. (2006). Identification of Nesfatin-1 as a Satiety Molecule in the Hypothalamus. Nature. 443: 709-712.
- 9. Liu J, Yong H, Liu Y, et al. (2019). Recent Advances in the Preparation, Sructural Characteristics, Biological Properties and Applications of Gallic Acid Grafted Polysaccharides. Int J Biol Macromol. DOI: 10.1016/j.ijbiomac..11.202.
- 10. Kahkeshani N, Farzaei F, Fotouhi M, et al. (2019). Pharmacological Effects of Gallic Acid in Health and Diseases: A Mechanistic Review. Iran J Basic Med Sci. 22(3): 225.
- 11. Badhani B, Sharma N, Kakkar R, et al. (2015). Gallic acid: a Versatile Antioxidant with Promising Therapeutic and Industrial Applications. Rsc Advances. 5(35): 27540-27557.
- 12. Choubey S, Varughese LR, Kumar V, et al. (2015). Medicinal Importance of Gallic Acid and Its Ester Derivatives: A Patent Review. Pharm Pat Anal. 4(4): 305-315.
- 13. Bahri S, Jameleddine S, Shlyonsky V. (2016). Relevance of Carnosic Acid to the Treatment of Several Health Disorders: Molecular Targets and Mechanisms. Biomed Pharmacother. 84: 569- 582.
- 14. Munné-Bosch S, Alegre L. (2000). Changes in Carotenoids, Tocopherols and Diterpenes During Drought and Recovery, and the Biological Significance of Chlorophyll Loss in Rosmarinus Officinalis Plants. Planta; 210(6): 925-931.
- 15. Fuhrman B, Volkova N, Rosenblat M, et al. (2000). Lycopene Synergistically İnhibits LDL Oxidation in Combination with Vitamin E, Glabridin, Rosmarinic Acid, Carnosic Acid, or Garlic. Antioxid Redox Signal. 2(3): 491-506.
- 16. Akalın PP, Bülbül B, Coyan K, et al. (2015). Relationship of Blood and Seminal Plasma Ceruloplasmin, Copper, Iron and Cadmium Concentrations with Sperm Quality in Merino Rams. Small Rumin Res. 133: 135-139.
- 17. Watson PF. (2000). The Causes of Reduced Fertility with Cryopreserved Semen. Anim Reprod Sci. 60: 481-492.
- 18. Câmara DR, Silva SV, Almeida FC, et al. (2011). Effects of Antioxidants and Duration of Pre-freezing Equilibration on FrozenThawed Ram Semen. Theriogenology. 76(2): 342-350.
- 19. Clarke R, Daly L, Robinson K, et al. (1991). Hyperhomocysteinemia: an Independent Risk Factor for Vascular Disease. N Eng J Med. 324(17): 1149–55.
- 20. Blom HJ, Kleinveld HA, Boers GH, et al. (1995). Lipid Peroxidation and Susceptibility of Lowdensity Lipoprotein to in Vitro Oxidation in Hyperhomocysteinemia. Eur J Clin Invest. 25(3): 149– 54.
- 21. Aitken RJ, West KM. (1990). Analysis of the Relationship between Reactive Oxygen Species Production and Leucocyte Infiltration in Fractions of Human Semen Separated on Percoll Gradients. Int J Androl. 13(6): 433-451.
- 22. Rezk BM, Khanna M, Dewitt S, et al. (2010). Homocysteine Reduces Sperm Motility via Elevation of Mitochondrial Superoxide Anions in Normal and Subfertile subjects: Potential Effect of Cotreatment with Folic acid. Free Radic Biol Med. 49: 195.
- 23. Ranjan A, Choubey M, Yada T, et al. (2019). Direct Effects of Neuropeptide Nesfatin-1 on Testicular Spermatogenesis and Steroidogenesis of the Adult Mice. Gen Comp Endocrinol. 271: 49-60.
- 24. Gao X, Zhang K, Song M, et al. (2016). Role of Nesfatin-1 in the Reproductive Axis of Male Rat. Sci Rep. 6: 32877.
- 25. Kuppan G, Balasubramanyam J, Monickaraj F, et al. (2010). Transcriptional Regulation of Cytokines and Oxidative Stressby Gallic Acid in Human THP-1 Monocytes. Cytokine. 49: 229-234.
- 26. Li D, Liu Z, Zhao W, et al. (2011). A Straightforward Method to Determine the Cytocidal and Cytopathic Effects of the Functional Groups of Gallic Acid. Process Biochem. 46: 2210–2214.
- 27. Nikseresht M, Fallahzadeh AR, Toori MA, et al. (2015). Effects of Pomegranate Seed Oil on the Fertilization Potency of Rat’s Sperm. J Clin Diagn Res. 9: 1-4.
- 28. Güngör Ș, Inanc ME, Ata A. (2019). Effect of Gallic Acid on Ram Semen Spermatological Parameters at +4° C Storage. Eurasian J Vet Sci. 35(2): 87-92.
- 29. Mehraban Z, Ghaffari Novin M, Golmohammadi MG, et al. (2019). Protective Effect of Gallic Acid on Apoptosis of Sperm and In Vitro Fertilization in Adult Male Mice Treated with Cyclophosphamide. J Cell Biochem. 120(10): 17250-17257.
- 30. Moreno S, Scheyer T, Romano CS, et al. (2006). Antioxidant and Antimicrobial Activities of Rosemary Extractslinked to Their Polyphenol Composition. Free Radic Res. 40: 223-231.
- 31. Yeni D, Inanc ME, Avdatek F, et al. (2018). Supplementation of Rosmarinic Acid has Reduced Oxidative Stress on Bull Spermatozoa Following the Freeze Thawing Process. CryoLett. 39: 156- 165.
- 32. Zanganeh Z, Zhandi M, Zare-Shahneh A, et al. (2013). Does Rosemary Aqueous Extract Improve Buck Semen Cryopreservation?. Small Rumin Res. 114(1): 120-125.
- 33. Gonzalez N, Gil L, Martinez F, et al. (2010). Effect of Natural Antioxidant Rosemary in Canine Soya Freezing Extender.Reprod Domest Anim. 45: 88.
- 34. Gil L, Mascaro F, Mur P, et al. (2010). Freezing Ram Semen: The Effect of Combination of Soya and Rose-Mary Essences as a Freezing Extender on Post-Thaw Sperm Motility. Reprod Domest Anim. 45: 91.
| APA | ÖZTÜRK C, GÜNGÖR Ş, İNANÇ M, AKSOY N (2020). Effects of Carnosic and Gallic Acid on Ram Sperm Parameters and Seminal Plasma Homocysteine-Nesfatin Levels after Thawing. , 104 - 107. 10.47027/duvetfd.753033 |
| Chicago | ÖZTÜRK Caner,GÜNGÖR Şükrü,İNANÇ MUHAMMED ENES,AKSOY NESE HAYAT Effects of Carnosic and Gallic Acid on Ram Sperm Parameters and Seminal Plasma Homocysteine-Nesfatin Levels after Thawing. (2020): 104 - 107. 10.47027/duvetfd.753033 |
| MLA | ÖZTÜRK Caner,GÜNGÖR Şükrü,İNANÇ MUHAMMED ENES,AKSOY NESE HAYAT Effects of Carnosic and Gallic Acid on Ram Sperm Parameters and Seminal Plasma Homocysteine-Nesfatin Levels after Thawing. , 2020, ss.104 - 107. 10.47027/duvetfd.753033 |
| AMA | ÖZTÜRK C,GÜNGÖR Ş,İNANÇ M,AKSOY N Effects of Carnosic and Gallic Acid on Ram Sperm Parameters and Seminal Plasma Homocysteine-Nesfatin Levels after Thawing. . 2020; 104 - 107. 10.47027/duvetfd.753033 |
| Vancouver | ÖZTÜRK C,GÜNGÖR Ş,İNANÇ M,AKSOY N Effects of Carnosic and Gallic Acid on Ram Sperm Parameters and Seminal Plasma Homocysteine-Nesfatin Levels after Thawing. . 2020; 104 - 107. 10.47027/duvetfd.753033 |
| IEEE | ÖZTÜRK C,GÜNGÖR Ş,İNANÇ M,AKSOY N "Effects of Carnosic and Gallic Acid on Ram Sperm Parameters and Seminal Plasma Homocysteine-Nesfatin Levels after Thawing." , ss.104 - 107, 2020. 10.47027/duvetfd.753033 |
| ISNAD | ÖZTÜRK, Caner vd. "Effects of Carnosic and Gallic Acid on Ram Sperm Parameters and Seminal Plasma Homocysteine-Nesfatin Levels after Thawing". (2020), 104-107. https://doi.org/10.47027/duvetfd.753033 |
| APA | ÖZTÜRK C, GÜNGÖR Ş, İNANÇ M, AKSOY N (2020). Effects of Carnosic and Gallic Acid on Ram Sperm Parameters and Seminal Plasma Homocysteine-Nesfatin Levels after Thawing. Dicle Üniversitesi Veteriner Fakültesi Dergisi, 13(2), 104 - 107. 10.47027/duvetfd.753033 |
| Chicago | ÖZTÜRK Caner,GÜNGÖR Şükrü,İNANÇ MUHAMMED ENES,AKSOY NESE HAYAT Effects of Carnosic and Gallic Acid on Ram Sperm Parameters and Seminal Plasma Homocysteine-Nesfatin Levels after Thawing. Dicle Üniversitesi Veteriner Fakültesi Dergisi 13, no.2 (2020): 104 - 107. 10.47027/duvetfd.753033 |
| MLA | ÖZTÜRK Caner,GÜNGÖR Şükrü,İNANÇ MUHAMMED ENES,AKSOY NESE HAYAT Effects of Carnosic and Gallic Acid on Ram Sperm Parameters and Seminal Plasma Homocysteine-Nesfatin Levels after Thawing. Dicle Üniversitesi Veteriner Fakültesi Dergisi, vol.13, no.2, 2020, ss.104 - 107. 10.47027/duvetfd.753033 |
| AMA | ÖZTÜRK C,GÜNGÖR Ş,İNANÇ M,AKSOY N Effects of Carnosic and Gallic Acid on Ram Sperm Parameters and Seminal Plasma Homocysteine-Nesfatin Levels after Thawing. Dicle Üniversitesi Veteriner Fakültesi Dergisi. 2020; 13(2): 104 - 107. 10.47027/duvetfd.753033 |
| Vancouver | ÖZTÜRK C,GÜNGÖR Ş,İNANÇ M,AKSOY N Effects of Carnosic and Gallic Acid on Ram Sperm Parameters and Seminal Plasma Homocysteine-Nesfatin Levels after Thawing. Dicle Üniversitesi Veteriner Fakültesi Dergisi. 2020; 13(2): 104 - 107. 10.47027/duvetfd.753033 |
| IEEE | ÖZTÜRK C,GÜNGÖR Ş,İNANÇ M,AKSOY N "Effects of Carnosic and Gallic Acid on Ram Sperm Parameters and Seminal Plasma Homocysteine-Nesfatin Levels after Thawing." Dicle Üniversitesi Veteriner Fakültesi Dergisi, 13, ss.104 - 107, 2020. 10.47027/duvetfd.753033 |
| ISNAD | ÖZTÜRK, Caner vd. "Effects of Carnosic and Gallic Acid on Ram Sperm Parameters and Seminal Plasma Homocysteine-Nesfatin Levels after Thawing". Dicle Üniversitesi Veteriner Fakültesi Dergisi 13/2 (2020), 104-107. https://doi.org/10.47027/duvetfd.753033 |
