Antibacterial and antifungal activity of MTA-basedroot canal sealer versus epoxy resin-based andmethacrylate resin-based sealers

Yıl: 2020 Cilt: 10 Sayı: 3 Sayfa Aralığı: 66 - 72 Metin Dili: İngilizce DOI: 10.5577/intdentres.2020.vol10.no3.1 İndeks Tarihi: 28-06-2021

Antibacterial and antifungal activity of MTA-basedroot canal sealer versus epoxy resin-based andmethacrylate resin-based sealers

Öz:
Aim: The purpose of this study was to compare the antibacterial and antifungal activity of MTA Fillapex with AH 26, AH Plus, and RealSeal root canal sealers. S. aureus, E. faecalis, and C. albicans were used as test microorganisms with the agar-diffusion test (ADT) and the direct contact test (DCT). Methodology: For the ADT, 48 Mueller-Hinton plates were divided into 3 groups according to the microorganism used. Each group was then divided into 4 subgroups according to root canal sealer. Mueller-Hinton and Sabouraud agar mediums were preferred, and inhibition zones were measured to determine the antimicrobial efficacy at designated intervals. In the DCT, 96-well microtiter plates were used. For each microorganism and each sealer, 8 consecutive wells were prepared vertically on the plate. Microbial suspensions were allowed to directly contact the sealers in each well for 1 hour at 37°C. Subsequently, microbial growth was spectrophotometrically measured at set intervals for the freshly mixed and set forms.Results: A statistically significant difference was found between the tested root canal sealers for antimicrobial effectiveness (p < 0.05). According to the ADT results, all sealers had antimicrobial activity against the tested microorganisms. MTA Fillapex demonstrated satisfying results in the ADT against all microorganisms. In the DCT, MTA Fillapex inhibited bacterial and fungal growth in all freshly mixed and set forms. However, the set forms of AH 26 and AH Plus began to lose their antimicrobial activity on the tested microorganisms after a while.Conclusion: The results showed that the MTA-based root canal sealer MTA Fillapex may be a favorable alternative sealer against bacterial and/or fungal species in clinical practice.
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  • 1. Ng YL, Mann V, Rahbaran S, Lewsey J, Gulabivala K. Outcome of primary root canal treatment: systematic review of the literature - Part 2. Influence of clinical factors. Int Endod J 2008; 41: 6-31. (Crossref)
  • 2. Mohammadi Z, Giardino L, Palazzi F, Shalavi S. Antibacterial activity of a new mineral trioxide aggregate-based root canal sealer. Int Dent J 2012; 62: 70-3. (Crossref)
  • 3. Gomes BP, Ferraz CC, Vianna ME, Rosalen PL, Zaia AA, Teixeira FB, et al. In vitro antimicrobial activity of calcium hydroxide pastes and their vehicles against selected microorganisms. Braz Dent J 2002; 13: 155-61. (Crossref)
  • 4. Smith AJ, Robertson D, Tang MK, Jackson MS, MacKenzie D, Bagg J. Staphylococcus aureus in the oral cavity: a three-year retrospective analysis of clinical laboratory data. Br Dental J 2003; 195: 701-3. (Crossref)
  • 5. Stuart CH, Schwartz SA, Beeson TJ, Owatz CB. Enterococcus faecalis: its role in root canal treatment failure and current concepts in retreatment. J Endod 2006; 32: 93-8. (Crossref)
  • 6. Portenier I, Waltimo TMT, Haapasalo M. Enterococcus faecalis: the root canal survivor and “star” in post-treatment disease. Endod Topics 2003; 6: 135-59. (Crossref)
  • 7. Siqueira JF, Sen BH. Fungi in endodontic infections. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2004; 97: 632-41. (Crossref)
  • 8. Baumgartner JC, Watts CM, Xia T. Occurrence of Candida albicans in infections of endodontic origin. J Endod 2000; 26: 695-8. (Crossref)
  • 9. Waltimo TM, Siren EK, Torkko HL, Olsen I, Haapasalo MP. Fungi in therapy-resistant apical periodontitis. Int Endod J 1997; 30: 96-101. (Crossref)
  • 10. Kaplan AE, Picca M, Gonzalez MI, Macchi RL, Molgatini SL. Antimicrobial effect of six endodontic sealers: an in vitro evaluation. Endod Dent Traumatol 1999; 15: 42-5.(Crossref)
  • 11. Bin CV, Valera MC, Camargo SE, Rabelo SB, Silva GO, Balducci I, et al. Cytotoxicity and genotoxicity of root canal sealers based on mineral trioxide aggregate. J Endod 2012; 38: 495- 500. (Crossref)
  • 12. Lodiene G, Morisbak E, Bruzell E, Orstavik D. Toxicity evaluation of root canal sealers in vitro. Int Endod J 2008; 41: 72-7.
  • 13. Assmann E, Scarparo RK, Böttcher DE, Grecca FS. Dentin bond strength of two mineral trioxide aggregate-based and one epoxy resin-based sealers. J Endod 2012; 38: 219-21. (Crossref)
  • 14. Sungur DD, Altundasar E, Uzunoglu E, Yilmaz Z. Influence of different final irrigation regiment and various endodontic filling materials on vertical root canal fracture resistance. Niger J Clin Pract 2016; 19: 267-71. (Crossref)
  • 15. Borges RP, Sousa- Neto MD, Versiani MA, Rached-Junior FA, DeDeus G, Miranda CE, et al. Changes in the surface of four calcium silicate‐containing endodontic materials and an epoxy resin‐based sealer after a solubility test. Int Endod J 2012; 45: 419-28. (Crossref)
  • 16. Morgental RD, Vier-Pelisser FV, Oliveira SD, Antunes FC, Cogo DM, Kopper PM. Antibacterial activity of two MTA-based root canal sealers. Int Endod J 2011; 44: 1128-33. (Crossref)
  • 17. Weiss EI, Shalhav M, Fuss Z. Assessment of antibacterial activity of endodontic sealers by a direct contact test. Endod Dent Traumatol 1996; 12: 179-84. (Crossref)
  • 18. Cobankara FK, Altinöz HC, Ergani O, Kav K, Belli S. In vitro antibacterial activities of root-canal sealers by using two different methods. J Endod. 2004; 30: 57-60. (Crossref)
  • 19. Spangberg L, Haapasalo H. Rationale and efficacy of root canal medicaments and root filling materials with emphasis on treatment outcome. Endod Topics 2002; 2: 35-58. (Crossref)
  • 20. Orstavik D. Antibacterial properties of root canal sealers, cements and pastes. Int Endod J 1981; 14: 125-33. (Crossref)
  • 21. Lai CC, Huang FM, Yang HW, Chan Y, Huang MS, Chou MY, Chang YC. Antimicrobial activity of four root canal sealers against endodontic pathogens. Clin Oral Investig 2001; 5: 236-9. (Crossref)
  • 22. Hubble TS, Hatton JF, Nallapareddy SR, Murray BE, Gillespie MJ. Influence of Enterococcus faecalis proteases and the collagen-binding protein, Ace, on adhesion to dentin. Oral Microbiol Immunol 2003; 18: 121-6. (Crossref)
  • 23. Peciuliene V, Reynaud AH, Balciuniene I, Haapasalo M. Isolation of yeasts and enteric bacteria in root-filled teeth with chronic apical periodontitis. Int Endod J 2001; 34: 429-34. (Crossref)
  • 24. Pizzo G, Giammanco GM, Cumbo E, Nicolosi G, Gallina G. In vitro antibacterial activity of endodontic sealers. J Dent 2006; 34: 35-40. (Crossref)
  • 25. Nawal RR, Parande M, Sehgal R, Naik A, Rao NR. A comparative evaluation of antimicrobial efficacy and flow properties for Epiphany, Guttaflow and AH-Plus sealer. Int Endod J 2011; 44: 307-13. (Crossref)
  • 26. Eldeniz AU, Erdemir A, Hadimli HH, Belli S, Erganis O. Assessment of antibacterial activity of EndoREZ. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006; 102: 119-26. (Crossref)
  • 27. Pizzo, G, Giammanco GM, Cumbo E, Nicolosi G, Gallina G. In vitro antibacterial activity of endodontic sealers. J Dent, 2006. 34: 35-40. (Crossref)
  • 28. Mickel AK, Nguyen TH, Chogle S. Antimicrobial activity of endodontic sealers on Enterococcus faecalis. J Endod, 2003; 29: 257-8. (Crossref)
  • 29. Poggio C, Lombardini M, Colombo M, Dagna A, Saino E, Arciola CR, et al. Antibacterial effects of six endodontic sealers. Int J Artif Organs 2011; 34: 908-13. (Crossref)
  • 30. Eldeniz AU, Hadimli HH, Ataoglu H, Orstavik D. Antibacterial effect of selected root-end filling materials. J Endod 2006; 32: 345-9. (Crossref)
  • 31. Roberts HW, Toth JM, Berzins DW, Charlton DG. Mineral trioxide aggregate material use in endodontic treatment: a review of the literature. Dent Mater 2008; 24: 149-64. (Crossref)
  • 32. Estrela C, Sydney GB, Bammann LL, Felippe Junior O. Mechanism of action of calcium and hydroxyl ions of calcium hydroxide on tissue and bacteria. Braz Dent J 1995; 6: 85-90.
  • 33. Borges RP, Sousa-Neto MD, Versiani MA, Rached-Hunior FA, DeDeus G, Miranda CE, et al. Changes in the surface of four calcium silicate-containing endodontic materials and an epoxy resin-based sealer after a solubility test. Int Endod J 2012; 45: 419-28. (Crossref)
  • 34. Faria-Junior NB, Tanomaru-Filho M, Berbert FL, GuerreiroTanomaru JM. Antibiofilm activity, pH and solubility of endodontic sealers. Int Endod J 2013; 46: 755-62. (Crossref)
  • 35. Torabinejad M, Hong CU, McDonald F, Pitt Ford TR. Physical and chemical properties of a new root-end filling material. J Endod 1995; 21: 349-53. (Crossref)
  • 36. Roggendorf MJ, Ebert J, Petschelt A, Frankenberger R. Influence of moisture on the apical seal of root canal fillings with five different types of sealer. J Endod 2007; 33: 31-3. (Crossref)
APA Turkyilmaz A, Erdemir A (2020). Antibacterial and antifungal activity of MTA-basedroot canal sealer versus epoxy resin-based andmethacrylate resin-based sealers. , 66 - 72. 10.5577/intdentres.2020.vol10.no3.1
Chicago Turkyilmaz Ali,Erdemir Ali Antibacterial and antifungal activity of MTA-basedroot canal sealer versus epoxy resin-based andmethacrylate resin-based sealers. (2020): 66 - 72. 10.5577/intdentres.2020.vol10.no3.1
MLA Turkyilmaz Ali,Erdemir Ali Antibacterial and antifungal activity of MTA-basedroot canal sealer versus epoxy resin-based andmethacrylate resin-based sealers. , 2020, ss.66 - 72. 10.5577/intdentres.2020.vol10.no3.1
AMA Turkyilmaz A,Erdemir A Antibacterial and antifungal activity of MTA-basedroot canal sealer versus epoxy resin-based andmethacrylate resin-based sealers. . 2020; 66 - 72. 10.5577/intdentres.2020.vol10.no3.1
Vancouver Turkyilmaz A,Erdemir A Antibacterial and antifungal activity of MTA-basedroot canal sealer versus epoxy resin-based andmethacrylate resin-based sealers. . 2020; 66 - 72. 10.5577/intdentres.2020.vol10.no3.1
IEEE Turkyilmaz A,Erdemir A "Antibacterial and antifungal activity of MTA-basedroot canal sealer versus epoxy resin-based andmethacrylate resin-based sealers." , ss.66 - 72, 2020. 10.5577/intdentres.2020.vol10.no3.1
ISNAD Turkyilmaz, Ali - Erdemir, Ali. "Antibacterial and antifungal activity of MTA-basedroot canal sealer versus epoxy resin-based andmethacrylate resin-based sealers". (2020), 66-72. https://doi.org/10.5577/intdentres.2020.vol10.no3.1
APA Turkyilmaz A, Erdemir A (2020). Antibacterial and antifungal activity of MTA-basedroot canal sealer versus epoxy resin-based andmethacrylate resin-based sealers. International Dental Research, 10(3), 66 - 72. 10.5577/intdentres.2020.vol10.no3.1
Chicago Turkyilmaz Ali,Erdemir Ali Antibacterial and antifungal activity of MTA-basedroot canal sealer versus epoxy resin-based andmethacrylate resin-based sealers. International Dental Research 10, no.3 (2020): 66 - 72. 10.5577/intdentres.2020.vol10.no3.1
MLA Turkyilmaz Ali,Erdemir Ali Antibacterial and antifungal activity of MTA-basedroot canal sealer versus epoxy resin-based andmethacrylate resin-based sealers. International Dental Research, vol.10, no.3, 2020, ss.66 - 72. 10.5577/intdentres.2020.vol10.no3.1
AMA Turkyilmaz A,Erdemir A Antibacterial and antifungal activity of MTA-basedroot canal sealer versus epoxy resin-based andmethacrylate resin-based sealers. International Dental Research. 2020; 10(3): 66 - 72. 10.5577/intdentres.2020.vol10.no3.1
Vancouver Turkyilmaz A,Erdemir A Antibacterial and antifungal activity of MTA-basedroot canal sealer versus epoxy resin-based andmethacrylate resin-based sealers. International Dental Research. 2020; 10(3): 66 - 72. 10.5577/intdentres.2020.vol10.no3.1
IEEE Turkyilmaz A,Erdemir A "Antibacterial and antifungal activity of MTA-basedroot canal sealer versus epoxy resin-based andmethacrylate resin-based sealers." International Dental Research, 10, ss.66 - 72, 2020. 10.5577/intdentres.2020.vol10.no3.1
ISNAD Turkyilmaz, Ali - Erdemir, Ali. "Antibacterial and antifungal activity of MTA-basedroot canal sealer versus epoxy resin-based andmethacrylate resin-based sealers". International Dental Research 10/3 (2020), 66-72. https://doi.org/10.5577/intdentres.2020.vol10.no3.1