Yıl: 2021 Cilt: 28 Sayı: 1 Sayfa Aralığı: 78 - 84 Metin Dili: İngilizce DOI: 10.5455/annalsmedres.2020.08.843 İndeks Tarihi: 28-06-2021

The preventive effect of sulforaphane on liver injury in a non-heart-beating donor model

Öz:
Aim: In this preliminary study, we aimed to investigate the effect of sulforaphane (SFN) warm ischemia in a non-heart beat rat liver model (NHBD). Materials and Methods: Livers taken from NHBD 15,30,60 min after warm ischemia were stored up at University of Wisconsin solution for 5 h at 40C [cold storage (CS)] and were, then, exposed to 2 h of machine reperfusion (MRP) at 370C. In the study group, SFN 5 mg/kg was intraperitoneal given 30 min before the introduction of NHB. Firstly, we divided the rats into the control and SFN groups. Then, each group was divided into three groups (n=10 in each) including control I, II, III and SFN I, II, III groups (15-, 30-, and 60-min warm ischemia period, respectively).Control and study groups at the same time of ischemia were compared with each other in terms of lactate, AST, ALT, IL-6, TNF-α and morphologically.Results: In the group treated with SFN, AST, ALT, TNF-α, IL-6, and lactate levels were lower, compared to the control group. Histopathologically, Bcl-2 staining intensity and percentages of staining were statistically significant, compared to the control and study groups during 15-min warm ischemia. Although not statistically significant, the intensity and percentage of staining were higher at 30 min and identical at 60 min. Conclusion: Our study results suggest that, despite prolonged warm ischemia time, SFN can biochemically and morphologically protect the liver from the hazardous effects of ischemia reperfusion in an experimentally-controlled NHB in rats, indicating an increased utilization rate of NHBDs.
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APA BAYKAN M, Yılmaz Z (2021). The preventive effect of sulforaphane on liver injury in a non-heart-beating donor model. , 78 - 84. 10.5455/annalsmedres.2020.08.843
Chicago BAYKAN MEHMET,Yılmaz Zeki The preventive effect of sulforaphane on liver injury in a non-heart-beating donor model. (2021): 78 - 84. 10.5455/annalsmedres.2020.08.843
MLA BAYKAN MEHMET,Yılmaz Zeki The preventive effect of sulforaphane on liver injury in a non-heart-beating donor model. , 2021, ss.78 - 84. 10.5455/annalsmedres.2020.08.843
AMA BAYKAN M,Yılmaz Z The preventive effect of sulforaphane on liver injury in a non-heart-beating donor model. . 2021; 78 - 84. 10.5455/annalsmedres.2020.08.843
Vancouver BAYKAN M,Yılmaz Z The preventive effect of sulforaphane on liver injury in a non-heart-beating donor model. . 2021; 78 - 84. 10.5455/annalsmedres.2020.08.843
IEEE BAYKAN M,Yılmaz Z "The preventive effect of sulforaphane on liver injury in a non-heart-beating donor model." , ss.78 - 84, 2021. 10.5455/annalsmedres.2020.08.843
ISNAD BAYKAN, MEHMET - Yılmaz, Zeki. "The preventive effect of sulforaphane on liver injury in a non-heart-beating donor model". (2021), 78-84. https://doi.org/10.5455/annalsmedres.2020.08.843
APA BAYKAN M, Yılmaz Z (2021). The preventive effect of sulforaphane on liver injury in a non-heart-beating donor model. Annals of Medical Research, 28(1), 78 - 84. 10.5455/annalsmedres.2020.08.843
Chicago BAYKAN MEHMET,Yılmaz Zeki The preventive effect of sulforaphane on liver injury in a non-heart-beating donor model. Annals of Medical Research 28, no.1 (2021): 78 - 84. 10.5455/annalsmedres.2020.08.843
MLA BAYKAN MEHMET,Yılmaz Zeki The preventive effect of sulforaphane on liver injury in a non-heart-beating donor model. Annals of Medical Research, vol.28, no.1, 2021, ss.78 - 84. 10.5455/annalsmedres.2020.08.843
AMA BAYKAN M,Yılmaz Z The preventive effect of sulforaphane on liver injury in a non-heart-beating donor model. Annals of Medical Research. 2021; 28(1): 78 - 84. 10.5455/annalsmedres.2020.08.843
Vancouver BAYKAN M,Yılmaz Z The preventive effect of sulforaphane on liver injury in a non-heart-beating donor model. Annals of Medical Research. 2021; 28(1): 78 - 84. 10.5455/annalsmedres.2020.08.843
IEEE BAYKAN M,Yılmaz Z "The preventive effect of sulforaphane on liver injury in a non-heart-beating donor model." Annals of Medical Research, 28, ss.78 - 84, 2021. 10.5455/annalsmedres.2020.08.843
ISNAD BAYKAN, MEHMET - Yılmaz, Zeki. "The preventive effect of sulforaphane on liver injury in a non-heart-beating donor model". Annals of Medical Research 28/1 (2021), 78-84. https://doi.org/10.5455/annalsmedres.2020.08.843