The preventive effect of sulforaphane on liver injury in a non-heart-beating donor model
Yıl: 2021 Cilt: 28 Sayı: 1 Sayfa Aralığı: 78 - 84 Metin Dili: İngilizce DOI: 10.5455/annalsmedres.2020.08.843 İndeks Tarihi: 28-06-2021
The preventive effect of sulforaphane on liver injury in a non-heart-beating donor model
Öz: Aim: In this preliminary study, we aimed to investigate the effect of sulforaphane (SFN) warm ischemia in a non-heart beat rat liver model (NHBD). Materials and Methods: Livers taken from NHBD 15,30,60 min after warm ischemia were stored up at University of Wisconsin solution for 5 h at 40C [cold storage (CS)] and were, then, exposed to 2 h of machine reperfusion (MRP) at 370C. In the study group, SFN 5 mg/kg was intraperitoneal given 30 min before the introduction of NHB. Firstly, we divided the rats into the control and SFN groups. Then, each group was divided into three groups (n=10 in each) including control I, II, III and SFN I, II, III groups (15-, 30-, and 60-min warm ischemia period, respectively).Control and study groups at the same time of ischemia were compared with each other in terms of lactate, AST, ALT, IL-6, TNF-α and morphologically.Results: In the group treated with SFN, AST, ALT, TNF-α, IL-6, and lactate levels were lower, compared to the control group. Histopathologically, Bcl-2 staining intensity and percentages of staining were statistically significant, compared to the control and study groups during 15-min warm ischemia. Although not statistically significant, the intensity and percentage of staining were higher at 30 min and identical at 60 min. Conclusion: Our study results suggest that, despite prolonged warm ischemia time, SFN can biochemically and morphologically protect the liver from the hazardous effects of ischemia reperfusion in an experimentally-controlled NHB in rats, indicating an increased utilization rate of NHBDs.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- 1. XianwaNiu, Wen Hua Huang, Bastiaan De Boer, et al.Iron-Induced Oxidative Rat Liver Injury After Non– Heart-Beating Warm Ischemia Is Mediatedby Tumor Necrosis Factor a and Prevented byDeferoxamin. LiverTranplatation 2014;20:904-11.
- 2. Guerrero-Beltrán CE, Calderón-Oliver M, Pedraza- ChaverriJ,et al. Protective effect of sulforaphane against oxidative stress: recentadvances. ExpToxicolPathol 2012; 64: 503-8.
- 3. Okada K, Warabi E, SugimotoH, et al. Nrf2 inhibits hepatic iron accumulation and counteracts oxidative stress-induced liver injury in nutritional steatohepatitis. J Gastroenterol 2012;47:924-35.
- 4. Seung-HaeBaek, Min Park, Jae-Hee Suh, et al. Protective effects of an extract of young radish (Raphanussativus L) cultivated with sulfur (sulfurradish extract) and of sulforaphane on carbon tetrachloride-induced hepatotoxicity. Biosci Biotechnol Biochem, 2008;72:1176-82.
- 5. Sue Kyung Yu, Gaab Soo Kim, Jun Young Chung. Anesthesia for liver transplantation from a maastricht category 4 non-heart-beating donor -A case report-. Korean Journal of Anesthsiology 2010;59:119-23.
- 6. Olivier de Rougemont, KunoLehmann, Pierre-Alain Clavien.Preconditioning, organ preservation, and postconditioning to prevent ischemia-reperfusion injury to the liver. Liver Transplantation 2009;15:1172- 82.
- 7. A Yalin, S Arbak, F Ercan. Preservatıon of lıver wıth euro-collıns solutıon: evaluatıon of ultrastructural alteratıons.MarmaraMedikal J 1999;12:29-32.
- 8. Karlsson JO. Cryopreservation; freezing and vitrifi cation. Science 2002;296:655-6.
- 9. JM Neuberger, WO Bechstein, DRJ Kuypers, et all. Practical Recommendations for Long-term Management of Modifiable Risks in Kidney and Liver Transplant Recipients. Transplantation 2017;101:1- 56.
- 10. JM O'Callaghan, RD Morgan, SR Knight, et al. Systematic review and meta-analysis of hypothermic machine perfusion versus static cold storage of kidney allografts on transplant outcomes.BJS 2013;100: 991-1001.
- 11. Belzer F, Ashby B, Dunphy J. 24-hour and 72-hour preservation of canine kidneys. Lancet 1967;2:536-8.
- 12. Karlsson JO. Cryopreservation; freezing and vitrifi cation. Science 2002;296:655-6.
- 13. Hai-Dong Zhao, Feng Zhang, Gang Shen, et. al, Sulforaphane protects liver injury induced by intestinal ischemia reperfusion through Nrf2-ARE pathway World J Gastroenterol 2010;16: 3002-10.
- 14. Reddy S, Zilvetti M, BrockmannJ, et al. Liver transplantation from non–heart–beating donors: current status and future prospects. Liver Transpl 2004;10:1223-32.
- 15. Fondevila C, Busuttil RW, Kupiec-Weglinski JW et al. Hepatic ischemia/reperfusion injury–a fresh look. ExpMolPathol 2003;74:86-93.
- 16. Dokumacioglu E, Iskender H, Aktas MS, et al.The effect of sulforaphane on oxidative stress and inflammationin rats with toxic hepatitis induced by acetaminophene.Bratislava Med J 2017;118:453 -9.
- 17. Li W, Chan AC, Lau YJ, et al. Superoxide and nitric oxide production by Kupffer cells in rats with obstructive jaundice: Effect of internal and external drainage. J GastroenterolHepatol 2004;19:160-5.
- 18. Rudiger HA, Clavien PA. Tumor necrosis factor alpha, but not Fas, mediates hepatocellular apoptosis in the murine ischemic liver. Gastroenterology 2002;122: 202-10.
- 19. Kong JS, Yoo SA, Kim HS, et al. Inhibition of synovial hyperplasia, rheumatoid T cell activation, and experimental arthritis in mice by sulforaphane, a naturally occurring isothiocyanate. Arthritis Rheum2010;62:159-70.
- 20. Chattopadhyay P, Chaudhury P ,Wahi K. Bcl-2 Expression Alters the Mitochondrial Tri Carboxyl Acid Pathway in Hepatic Ischemic and ReperfusionInduced Necrosis and Apoptosis in Rat Liver. Indian J Pharm Sci 2010;72:437-41.
- 21. Gapany C, Zhao M, Zimmermann A, et al. The apoptosis protector, bcl-2 protein, is downregulated in bile duct epithelial cells of human liver allografts. Hepatology1997;26:535-42.
- 22. Cetin M, Yİlmaz Z, ArtisT, et al.The Effect of Edavaron's Liver Damage in an Experimental Non-Heart Beating Donor Model. Erciyes Med J 2012;34:127-31.
APA | BAYKAN M, Yılmaz Z (2021). The preventive effect of sulforaphane on liver injury in a non-heart-beating donor model. , 78 - 84. 10.5455/annalsmedres.2020.08.843 |
Chicago | BAYKAN MEHMET,Yılmaz Zeki The preventive effect of sulforaphane on liver injury in a non-heart-beating donor model. (2021): 78 - 84. 10.5455/annalsmedres.2020.08.843 |
MLA | BAYKAN MEHMET,Yılmaz Zeki The preventive effect of sulforaphane on liver injury in a non-heart-beating donor model. , 2021, ss.78 - 84. 10.5455/annalsmedres.2020.08.843 |
AMA | BAYKAN M,Yılmaz Z The preventive effect of sulforaphane on liver injury in a non-heart-beating donor model. . 2021; 78 - 84. 10.5455/annalsmedres.2020.08.843 |
Vancouver | BAYKAN M,Yılmaz Z The preventive effect of sulforaphane on liver injury in a non-heart-beating donor model. . 2021; 78 - 84. 10.5455/annalsmedres.2020.08.843 |
IEEE | BAYKAN M,Yılmaz Z "The preventive effect of sulforaphane on liver injury in a non-heart-beating donor model." , ss.78 - 84, 2021. 10.5455/annalsmedres.2020.08.843 |
ISNAD | BAYKAN, MEHMET - Yılmaz, Zeki. "The preventive effect of sulforaphane on liver injury in a non-heart-beating donor model". (2021), 78-84. https://doi.org/10.5455/annalsmedres.2020.08.843 |
APA | BAYKAN M, Yılmaz Z (2021). The preventive effect of sulforaphane on liver injury in a non-heart-beating donor model. Annals of Medical Research, 28(1), 78 - 84. 10.5455/annalsmedres.2020.08.843 |
Chicago | BAYKAN MEHMET,Yılmaz Zeki The preventive effect of sulforaphane on liver injury in a non-heart-beating donor model. Annals of Medical Research 28, no.1 (2021): 78 - 84. 10.5455/annalsmedres.2020.08.843 |
MLA | BAYKAN MEHMET,Yılmaz Zeki The preventive effect of sulforaphane on liver injury in a non-heart-beating donor model. Annals of Medical Research, vol.28, no.1, 2021, ss.78 - 84. 10.5455/annalsmedres.2020.08.843 |
AMA | BAYKAN M,Yılmaz Z The preventive effect of sulforaphane on liver injury in a non-heart-beating donor model. Annals of Medical Research. 2021; 28(1): 78 - 84. 10.5455/annalsmedres.2020.08.843 |
Vancouver | BAYKAN M,Yılmaz Z The preventive effect of sulforaphane on liver injury in a non-heart-beating donor model. Annals of Medical Research. 2021; 28(1): 78 - 84. 10.5455/annalsmedres.2020.08.843 |
IEEE | BAYKAN M,Yılmaz Z "The preventive effect of sulforaphane on liver injury in a non-heart-beating donor model." Annals of Medical Research, 28, ss.78 - 84, 2021. 10.5455/annalsmedres.2020.08.843 |
ISNAD | BAYKAN, MEHMET - Yılmaz, Zeki. "The preventive effect of sulforaphane on liver injury in a non-heart-beating donor model". Annals of Medical Research 28/1 (2021), 78-84. https://doi.org/10.5455/annalsmedres.2020.08.843 |