The Effect of Feeding with Resveratrol on Myogenin and mTOR Levels in Mice
Yıl: 2020 Cilt: 48 Sayı: 2 Sayfa Aralığı: 52 - 57 Metin Dili: İngilizce DOI: 0.33076/2020.BDD.1351 İndeks Tarihi: 29-06-2021
The Effect of Feeding with Resveratrol on Myogenin and mTOR Levels in Mice
Öz: Aim: It is reported that resveratrol may induce muscle hypertrophy and myogenesis. Accordingly, resveratrol is promising forthe treatment of muscle diseases and ergogenic aid for athletes. In this study it is aimed to observe the effects of resveratrolon myogenin and mTOR levels and muscle mass.Material and Methods: The study is performed on 14 Swiss albino young adult mice at Başkent University ExperimentalAnimal Research Center. The mice are divided randomly into study (n:7) and control (n:7) groups. During consecutive 7 days,20 mg/kg trans-resveratrol was given to the study group intraperitoneally. After the seventh day administration, the rightgastrocnemius muscles are disected, weighted and stored at -80 °C for mTOR and myogenin analysis. Then, myogenin andmTOR levels are determined by enzyme-linked immunosorbent assay (ELISA) method in homogenised muscle mass.Results: According to mean myogenin and mTOR levels, there was no significantly difference between groups (p>0.05). Totalbody weight and the right gastrocnemius muscle weight of the study group (22.11±3.22 g, 0.09±0.03 g, respectively) at the 7thday was found significantly lower than control group (28.63±3.80 g, 0.12±0.02 g, respectively) (p<0.05).Conclusion: It was observed that resveratrol administration had no effect on myogenin and mTOR levels which is a fore-stepfor muscle hypertrophy. On the other hand, interestingly total body weight and muscle mass decrease was observed in thestudy group. The effects of resveratrol on muscle tissue is a relatively less studied topic. However, the authors are suggestingmore studies on the resveratrol usage or administration for the effective dose and time interval investigation.
Anahtar Kelime: Resveratrol ile Beslenmenin Farelerde Myogenin ve mTOR Düzeyleri Üzerine Etkisi
Öz: Amaç: Resveratrolün kas hipertrofisi ve miyogenezi indükleyebileceği bildirilmektedir. Bu nedenle, resveratrol kas hastalıklarının tedavisi ve sporcular için ergojenik yardım olarak umut vermektedir. Mevcut çalışmada, resveratrolün miyogenin ve mTOR düzeyleri ve kas kütlesi üzerindeki etkilerinin gözlenmesi amaçlanmıştır. Gereç ve Yöntem: Çalışma, Başkent Üniversitesi Deney Hayvanları Araştırma Merkezi’nde 14 Swiss albino genç erişkin fare üzerinde yürütülmüştür. Fareler rastgele olarak çalışma (n:7) ve kontrol grubuna (n:7) ayrılmıştır. Çalışma grubuna ardışık 7 gün boyunca 20 mg/kg transresveratrol intraperitoneal yol ile verilmiştir. Yedinci gün uygulamasından sonra farelerin gastroknemius kas diseksiyonları yapılarak, ağırlıkları kaydedilmiş ve miyogenin, mTOR analizleri için -80 °C’de saklanmıştır. Myogenin ve mTOR düzeyleri homojenize kas dokusundan enzim bağlı immunosorbent analizi (ELISA) yöntemi kullanılarak saptanmıştır. Bulgular: Gruplar arasında miyogenin ve mTOR düzey ortalamaları açısından anlamlı farklılık saptanmamıştır (p>0.05). Çalışma grubunun vücut ağırlığı ve sağ gastroknemius kasının 7. gün ağırlığı (sırasıyla, 22.11±3.22 g, 0.09±0.03 g), kontrol grubuna göre anlamlı derecede düşük bulunmuştur (sırasıyla, 28.63±3.80 g, 0.12±0.02 g) (p<0.05). Sonuç: Bu çalışmada resveratrol uygulamasının kas hipertrofisi için önemli bir basamak olan miyogenin ve mTOR düzeyleri üzerinde bir etkisinin olmadığı gözlenmiştir. Buna ek olarak çalışma grubunda ilginç olarak vücut ve kas dokusu ağırlığı daha düşük saptanmıştır. Resveratrolün kas dokusu üzerindeki etkileri nispeten daha az çalışılmış bir konudur bu nedenle etkilerinin belirlenebilmesi için etkili doz ve zaman aralığı hakkında daha fazla çalışmaya ihtiyaç vardır.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | ANDAC OZTURK S, Kızıltan G, yurtcu e, Bacanlı D, KORKMAZ Y, Köse B (2020). The Effect of Feeding with Resveratrol on Myogenin and mTOR Levels in Mice. , 52 - 57. 0.33076/2020.BDD.1351 |
| Chicago | ANDAC OZTURK SERAP,Kızıltan Gül,yurtcu erkan,Bacanlı Didem,KORKMAZ YESIM,Köse Beril The Effect of Feeding with Resveratrol on Myogenin and mTOR Levels in Mice. (2020): 52 - 57. 0.33076/2020.BDD.1351 |
| MLA | ANDAC OZTURK SERAP,Kızıltan Gül,yurtcu erkan,Bacanlı Didem,KORKMAZ YESIM,Köse Beril The Effect of Feeding with Resveratrol on Myogenin and mTOR Levels in Mice. , 2020, ss.52 - 57. 0.33076/2020.BDD.1351 |
| AMA | ANDAC OZTURK S,Kızıltan G,yurtcu e,Bacanlı D,KORKMAZ Y,Köse B The Effect of Feeding with Resveratrol on Myogenin and mTOR Levels in Mice. . 2020; 52 - 57. 0.33076/2020.BDD.1351 |
| Vancouver | ANDAC OZTURK S,Kızıltan G,yurtcu e,Bacanlı D,KORKMAZ Y,Köse B The Effect of Feeding with Resveratrol on Myogenin and mTOR Levels in Mice. . 2020; 52 - 57. 0.33076/2020.BDD.1351 |
| IEEE | ANDAC OZTURK S,Kızıltan G,yurtcu e,Bacanlı D,KORKMAZ Y,Köse B "The Effect of Feeding with Resveratrol on Myogenin and mTOR Levels in Mice." , ss.52 - 57, 2020. 0.33076/2020.BDD.1351 |
| ISNAD | ANDAC OZTURK, SERAP vd. "The Effect of Feeding with Resveratrol on Myogenin and mTOR Levels in Mice". (2020), 52-57. https://doi.org/0.33076/2020.BDD.1351 |
| APA | ANDAC OZTURK S, Kızıltan G, yurtcu e, Bacanlı D, KORKMAZ Y, Köse B (2020). The Effect of Feeding with Resveratrol on Myogenin and mTOR Levels in Mice. Beslenme ve Diyet Dergisi, 48(2), 52 - 57. 0.33076/2020.BDD.1351 |
| Chicago | ANDAC OZTURK SERAP,Kızıltan Gül,yurtcu erkan,Bacanlı Didem,KORKMAZ YESIM,Köse Beril The Effect of Feeding with Resveratrol on Myogenin and mTOR Levels in Mice. Beslenme ve Diyet Dergisi 48, no.2 (2020): 52 - 57. 0.33076/2020.BDD.1351 |
| MLA | ANDAC OZTURK SERAP,Kızıltan Gül,yurtcu erkan,Bacanlı Didem,KORKMAZ YESIM,Köse Beril The Effect of Feeding with Resveratrol on Myogenin and mTOR Levels in Mice. Beslenme ve Diyet Dergisi, vol.48, no.2, 2020, ss.52 - 57. 0.33076/2020.BDD.1351 |
| AMA | ANDAC OZTURK S,Kızıltan G,yurtcu e,Bacanlı D,KORKMAZ Y,Köse B The Effect of Feeding with Resveratrol on Myogenin and mTOR Levels in Mice. Beslenme ve Diyet Dergisi. 2020; 48(2): 52 - 57. 0.33076/2020.BDD.1351 |
| Vancouver | ANDAC OZTURK S,Kızıltan G,yurtcu e,Bacanlı D,KORKMAZ Y,Köse B The Effect of Feeding with Resveratrol on Myogenin and mTOR Levels in Mice. Beslenme ve Diyet Dergisi. 2020; 48(2): 52 - 57. 0.33076/2020.BDD.1351 |
| IEEE | ANDAC OZTURK S,Kızıltan G,yurtcu e,Bacanlı D,KORKMAZ Y,Köse B "The Effect of Feeding with Resveratrol on Myogenin and mTOR Levels in Mice." Beslenme ve Diyet Dergisi, 48, ss.52 - 57, 2020. 0.33076/2020.BDD.1351 |
| ISNAD | ANDAC OZTURK, SERAP vd. "The Effect of Feeding with Resveratrol on Myogenin and mTOR Levels in Mice". Beslenme ve Diyet Dergisi 48/2 (2020), 52-57. https://doi.org/0.33076/2020.BDD.1351 |
