Analyses of the relationships among biofilm activity, antibiotic resistance and expressions of SHV, TEM, CTXand IntI in Klebsiella pneumoniae

KILINC, CETİN; Güçkan, Rıdvan; Başkan, ceren; Yıldırım, Tuba; YAZGAN, BURAK; Mesci, Seda

doi:10.5455/annalsmedres.2020.03.211

Analyses of the relationships among biofilm activity, antibiotic resistance and expressions of SHV, TEM, CTXand IntI in Klebsiella pneumoniae

Rıdvan GÜÇKAN, (Amasya Sabuncuoğlu Şerefeddin Eğitim ve Araştırma Hastanesi, Mikrobiyoloji Laboratuvarı, Amasya, Türkiye)

Çetin KILINÇ, (Amasya Sabuncuoğlu Şerefeddin Eğitim ve Araştırma Hastanesi, Mikrobiyoloji Laboratuvarı, Amasya, Türkiye)

Ceren BAŞKAN, (Amasya Üniversitesi, Sabuncuoğlu Şerefeddin Sağlık Hizmetleri Meslek Yüksekokulu, Fizik Tedavi ve Rehabilitasyon Bölümü, Amasya, Türkiye)

Burak YAZGAN, (Amasya Üniversitesi, Sabuncuoğlu Şerefeddin Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Hizmetler ve Teknikler Bölümü, Amasya, Türkiye)

Seda MESCİ, (Hitit Üniversitesi, Tıp Fakültesi, Tıbbi Biyokimya Anabilim Dalı, Çorum, Türkiye)

Tuba YILDIRIM (Amasya Üniversitesi, Fen-Edebiyat Fakültesi, Biyoloji Bölümü, Amasya, Türkiye)

Annals of Medical Research

4 3

Yıl: 2021 Cilt: 28 Sayı: 1 Sayfa Aralığı: 197 - 203 Metin Dili: İngilizce DOI: 10.5455/annalsmedres.2020.03.211 İndeks Tarihi: 30-06-2021

Analyses of the relationships among biofilm activity, antibiotic resistance and expressions of SHV, TEM, CTXand IntI in Klebsiella pneumoniae

Öz:

Aim: Klebsiella pneumoniae as an opportunistic pathogen is responsible for nosocomial, urinary tract infections, pneumonia, bacteremia, liver abscesses, and respiratory and blood infections in patients. The aim of our study is to examine the antibiotic sensitivity, expression levels of IntI, TEM, SHV and CTX genes, which play a role in multidrug resistance, biofilm formation and even the relationships among them. Materials and Methods: Identification was implemented by carrying out the VITEK2 system and phenotypic confirmation of the ESBL producing isolates by using a combined disc test. Antibiotic sensitivity was implemented by disc diffusion method and microdilution methods toward various antibiotics. Furthermore, biofilm formation was examined through microtitration plate method. Results: The expressions of IntI, TEM, SHV and CTX were implemented in qRT-PCR. 19 were susceptible to all antibiotics, 17 were ESBL (+) and 16 were carbapenem-resistant among 52. The expression of these genes was upregulated in ESBL (+) strains and produced biofilm and the expression of these genes was upregulated, too. Conclusion: The results of the study revealed the potential role of the mentioned genes in biofilm formation and antibiotic resistance, enabling the development of new drugs. Further studies will be essential so as to determine when or how these systems are included in antibiotic resistance and biofilm formation.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Flemming HC, Wingender J. The biofilm matrix. Nat Rev Microbiol 2010;8:623-33.
2. Fuqua WC, Winans SC, Greenberg EP. Quorum sensing in bacteria: the LuxR-LuxI family of cell densityresponsive transcriptional regulators. J Bacteriol 1994;176:269–75.
3. Hassan A, Usman J, Kaleem F, et al. Evaluation of different detection methods of biofilm formation in the clinical isolates. Braz J Infect Dis 2011;15:305-11.
4. Brisse S, Grimont F, Grimont PA. The genus Klebsiella. Prokaryotes 2006;6:159–96.
5. Yang HS, Fang WL, Lin YT. A case of liver abscess caused by tigecycline-nonsusceptible Klebsiella pneumoniae. J Microbiol Immunol Infect 2016;49:621- 2.
6. Dehshiri M, Khoramrooz SS, Zolad M, et al. The frequency of Klebsiella pneumonia encoding genes for CTX-M, TEM-1 and SHV-1 extended-spectrum beta lactamases enzymes isolated from urinary tract infection. Ann Clin Microbiol Antimicrob 2018;17:1-7.
7. Vuotto C, Longo F, Pascolini C, et al. Bioﬁlm formation and antibiotic resistance in Klebsiella pneumoniae urinary strains. J Appl Microbiol 2017;123:1003-18.
8. Markowitz SM, Veazey JM Jr, Macrina FL, et al. Sequential outbreaks of infection due to Klebsiella pneumoniae in a neonatal intensive care unit: implication of a conjugative R plasmid. J Infect Dis 1980;142:106–12.
9. Younis G, Awad A, Ashraf N. Molecular and phenotypic characterization of antimicrobial resistance in Gram negatife bacteria recovered from subclinical mastitis. Adv Anim Vet Sci 2017;5:196-204.
10. Gharrah MM, El-Mahdy AM, Barwa RF. Association between virulence factors and extended spectrum beta-lactamase producing Klebsiella pneumoniae compared to nonproducing ısolate. Interdiscip Perspect Infect Dis 7279830; 2017. p.14.
11. Mobarak-Qamsari M, Ashayeri-Panah M, Eftekhar F, et al. Integron mediated multidrug resistance in extended-spectrum beta-lactamase producing clinical isolates of Klebsiella pneumoniae. Braz J Microbiol 2013;44:849-54.
12. Hennequin C, Robin F. Correlation between antimicrobial resistance and virulence in Klebsiella pneumoniae. Eur J Clin Microbiol Infect Dis 2016;35:333–41.
13. Shaikh S, Rizvi SMD, Anis R, et al. Prevalence of CTX-M resistance marker and integrons among Escherichia coli and Klebsiella pneumoniae isolates of clinical origin. Lett Appl Microbiol 2016;62:419-27.
14. Clinical and Laboratory Standards Institute (CLSI) Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard, 10th ed. CLSI document M07-A10. Clinical and Laboratory Standards Institute, Wayne, PA.;2015.
15. O’Toole GA, Kolter R. Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signal ling pathways. Mol Microbiol 1998;28:449–61.
16. Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative C (T) method. Nat Protoc 2008;3:1101-8.
17. Lübbert C, Straube L, Stein C, et al. Colonization with extended-spectrum beta-lactamase-producing and carbapenemase-producing Enterobacteriaceae in international travelers returning to Germany. Int J Med Microbiol 2015;305:148-56.
18. Pitout JD, Nordmann P, Poirel L. Carbapenemaseproducing Klebsiella pneumoniae, a keypathogen set for global nosocomial dominance. Antimicrob Agents Chemother 2015;59:5873-84.
19. Ghasemi Y, Archin T, Kargar M, et al. A simple multiplex PCR for assessing prevalence of extended-spectrum β-lactamases producing Klebsiella pneumoniae in Intensive Care Units of a referral hospital in Shiraz, Iran. Asian Pac J Trop Med 2013;6:703-8
20. Gürntke S, Kohler C, Steinmetz I, et al. Molecular epidemiology of extended-spectrum beta-lactamase (ESBL)-positive Klebsiella pneumoniae from bloodstream infections and risk factors for mortality. J Infect Chemother 2014;20:817-9.
21. Ali Abdel Rahim KA, Ali Mohamed AM. Prevalence of extended-spectrum β-lactamase-producing Klebsiella pneumoniae in clinical isolates. Jundishapur J Microbiol 2014;7: e17114.
22. Brizendine KD, Richter SS, Cober ED, et al. Carbapenemresistant Klebsiella pneumoniae urinary tract infection following solid organ transplantation. Antimicrob Agents Chemother 2015;59:553-7.
23. Molton JS, Tambyah PA, Ang BS, et al. The global spread of healthcare-associated multidrug-resistant bacteria: a perspective from Asia. Clin Infect Dis 2013;56:1310-8.
24. Poirel L, Jayol A, Bontron S, et al. The mgrB gene as a key target for acquired resistance to colistin in Klebsiella pneumoniae. J Antimicrob Chemother 2015;70:75-80.
25. Olsen I. Biofilm-specific antibiotic tolerance and resistance. Eur J Clin Microbiol Infect Dis 2015;34:877–86.
26. Bellifa S, Hassaine H, Balestrino D, et al. Evaluation of biofilm formation of Klebsiella pneumoniae isolated from medical devices at the University Hospital of Tlemcen, Algeria, Afr J Microbiol Res 2013;7:5558–64.
27. Yazgan B, Türkel İ, Güçkan R, et al. Comparison of biofilm formation and efflux pumps in ESBL and carbapenemase producing Klebsiella pneumoniae. J Infect Dev Ctries 2018;12:156-63.
28. Yang D, Zhang Z. Bioﬁlm-forming Klebsiella pneumoniae strains have greater likelihood of producing extended-spectrum beta-lactamases. J Hosp Infect 2008;68:369–71.
29. Sanchez CJ Jr, Mende K, Beckius ML, et al. Bioﬁlm formation by clinical isolates and the implications in chronic infections. BMC Infect Dis 2013;29:13–47.
30. May T, Ito A, Okabe S. Induction of multidrug resistance mechanism in Escherichia coli bioﬁlms by interplay between tetracycline and ampicillin resistance genes, Antimicrob Agents Chemother 2009;53:4628–39.
31. Khemtong S, Chuanchuen R. Class 1 integrons and Salmonella genomic island 1 among Salmonella enterica isolated from poultry and swine. Microb Drug Resist 2008;14:65–70.
32. Saha MR, Jhora ST, Khan TM, et al. Detection of SHV gene from extended-spectrum beta-lactamases (ESBLs) producing ısolates in a Tertiary Care hospital. Bangladesh J Med Microbiol 2014;8:14-8.
33. Aljanaby AAJ, Alhasani AHA. Virulence factors and antibiotic susceptibility patterns of multidrug resistance Klebsiella pneumoniae isolated from different clinical infections. Afr J Microbiol Res 2016;10:829–43.
34. Dropa M, Balsalobre LC, Matté LN, et al. Complex class 1 integrons harboring CTX-M-2-encoding genes in clinical Enterobacteriaceae from a hospital in Brazil. J Infect Dev Ctries 2015;9:890-7.
35. Wang G, Huang T, Surendraiah PKM, et al. CTXM b-Lactamase–producing Klebsiella pneumoniae in Suburban New York, USA. Emerg Infect Di. 2013;19:1803–10.
36. Ashayeri-Panah M, Feizabadi MM, Eftekhar F. Correlation of multi-drug resistance, integron and blaESBL gene carriage with genetic fingerprints of extended-spectrum β-Lactamase producing Klebsiella pneumoniae. Jundishapur J Microbiol 2014;7:1-5.
37. Moeen F, Jarallah AEM. Distribution of TEM β-Lactamase gene among carbapenem resistant clinical isolates of Klebsiella pneumoniae in Hilla Hospitals. Al-Kufa Univ J Bio 2016;8:172-80.
38. Khorshidi A, Rohani M, Moniri R. The prevalence and molecular characterization of extended- spectrum β-lactamases producing Klebsiella pneumoniae isolates recovered from Kashan Hospital University, Iran. Jundishapur J Microbiol 2011;4:289- 94.
39. Opuş A, Yıldırım T, Birinci A. et al. Investigation of the relationship between class-1 integrons and per- 1 enzyme in ceftazidime resistant Pseudomonas aeruginosa. Biomed Res 2017;28:2689-95.

APA	Güçkan R, KILINC C, Başkan c, YAZGAN B, Mesci S, Yıldırım T (2021). Analyses of the relationships among biofilm activity, antibiotic resistance and expressions of SHV, TEM, CTXand IntI in Klebsiella pneumoniae. , 197 - 203. 10.5455/annalsmedres.2020.03.211
Chicago	Güçkan Rıdvan,KILINC CETİN,Başkan ceren,YAZGAN BURAK,Mesci Seda,Yıldırım Tuba Analyses of the relationships among biofilm activity, antibiotic resistance and expressions of SHV, TEM, CTXand IntI in Klebsiella pneumoniae. (2021): 197 - 203. 10.5455/annalsmedres.2020.03.211
MLA	Güçkan Rıdvan,KILINC CETİN,Başkan ceren,YAZGAN BURAK,Mesci Seda,Yıldırım Tuba Analyses of the relationships among biofilm activity, antibiotic resistance and expressions of SHV, TEM, CTXand IntI in Klebsiella pneumoniae. , 2021, ss.197 - 203. 10.5455/annalsmedres.2020.03.211
AMA	Güçkan R,KILINC C,Başkan c,YAZGAN B,Mesci S,Yıldırım T Analyses of the relationships among biofilm activity, antibiotic resistance and expressions of SHV, TEM, CTXand IntI in Klebsiella pneumoniae. . 2021; 197 - 203. 10.5455/annalsmedres.2020.03.211
Vancouver	Güçkan R,KILINC C,Başkan c,YAZGAN B,Mesci S,Yıldırım T Analyses of the relationships among biofilm activity, antibiotic resistance and expressions of SHV, TEM, CTXand IntI in Klebsiella pneumoniae. . 2021; 197 - 203. 10.5455/annalsmedres.2020.03.211
IEEE	Güçkan R,KILINC C,Başkan c,YAZGAN B,Mesci S,Yıldırım T "Analyses of the relationships among biofilm activity, antibiotic resistance and expressions of SHV, TEM, CTXand IntI in Klebsiella pneumoniae." , ss.197 - 203, 2021. 10.5455/annalsmedres.2020.03.211
ISNAD	Güçkan, Rıdvan vd. "Analyses of the relationships among biofilm activity, antibiotic resistance and expressions of SHV, TEM, CTXand IntI in Klebsiella pneumoniae". (2021), 197-203. https://doi.org/10.5455/annalsmedres.2020.03.211

APA	Güçkan R, KILINC C, Başkan c, YAZGAN B, Mesci S, Yıldırım T (2021). Analyses of the relationships among biofilm activity, antibiotic resistance and expressions of SHV, TEM, CTXand IntI in Klebsiella pneumoniae. Annals of Medical Research, 28(1), 197 - 203. 10.5455/annalsmedres.2020.03.211
Chicago	Güçkan Rıdvan,KILINC CETİN,Başkan ceren,YAZGAN BURAK,Mesci Seda,Yıldırım Tuba Analyses of the relationships among biofilm activity, antibiotic resistance and expressions of SHV, TEM, CTXand IntI in Klebsiella pneumoniae. Annals of Medical Research 28, no.1 (2021): 197 - 203. 10.5455/annalsmedres.2020.03.211
MLA	Güçkan Rıdvan,KILINC CETİN,Başkan ceren,YAZGAN BURAK,Mesci Seda,Yıldırım Tuba Analyses of the relationships among biofilm activity, antibiotic resistance and expressions of SHV, TEM, CTXand IntI in Klebsiella pneumoniae. Annals of Medical Research, vol.28, no.1, 2021, ss.197 - 203. 10.5455/annalsmedres.2020.03.211
AMA	Güçkan R,KILINC C,Başkan c,YAZGAN B,Mesci S,Yıldırım T Analyses of the relationships among biofilm activity, antibiotic resistance and expressions of SHV, TEM, CTXand IntI in Klebsiella pneumoniae. Annals of Medical Research. 2021; 28(1): 197 - 203. 10.5455/annalsmedres.2020.03.211
Vancouver	Güçkan R,KILINC C,Başkan c,YAZGAN B,Mesci S,Yıldırım T Analyses of the relationships among biofilm activity, antibiotic resistance and expressions of SHV, TEM, CTXand IntI in Klebsiella pneumoniae. Annals of Medical Research. 2021; 28(1): 197 - 203. 10.5455/annalsmedres.2020.03.211
IEEE	Güçkan R,KILINC C,Başkan c,YAZGAN B,Mesci S,Yıldırım T "Analyses of the relationships among biofilm activity, antibiotic resistance and expressions of SHV, TEM, CTXand IntI in Klebsiella pneumoniae." Annals of Medical Research, 28, ss.197 - 203, 2021. 10.5455/annalsmedres.2020.03.211
ISNAD	Güçkan, Rıdvan vd. "Analyses of the relationships among biofilm activity, antibiotic resistance and expressions of SHV, TEM, CTXand IntI in Klebsiella pneumoniae". Annals of Medical Research 28/1 (2021), 197-203. https://doi.org/10.5455/annalsmedres.2020.03.211