Assessment of the Immunogenicity and Protective Aspects of a DNA Vaccine Targeting Crimean Congo Hemorrhagic Fever Virus Glycoprotein Gc

Ozkul, Aykut; Coleri Cihan, Arzu; Engin, Doruk; Földes, Katalin; A.Farzani, Touraj; Sahin, Ergin

doi:10.18678/dtfd.864114

Assessment of the Immunogenicity and Protective Aspects of a DNA Vaccine Targeting Crimean Congo Hemorrhagic Fever Virus Glycoprotein Gc

Ergin ŞAHİN, (Ankara Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Ankara Türkiye)

Arzu ÇÖLERİ CİHAN, (Ankara Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Ankara Türkiye)

Touraj ALİGHOLİPOUR FARZANİ, (Ankara Üniversitesi, Veteriner Fakültesi, Viroloji Anabilim Dalı, Ankara, Türkiye)

Katalin FÖLDES, (Ankara Üniversitesi, Veteriner Fakültesi, Viroloji Anabilim Dalı, Ankara, Türkiye)

Evren Doruk ENGİN, (Ankara Üniversitesi, Biyoteknoloji Enstitüsü, Biyoteknoloji Bölümü, Ankara, Türkiye)

Aykut ÖZKUL (Ankara Üniversitesi, Veteriner Fakültesi, Viroloji Anabilim Dalı, Ankara, Türkiye)

Düzce Tıp Fakültesi Dergisi

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Yıl: 2021 Cilt: 23 Sayı: 1 Sayfa Aralığı: 66 - 75 Metin Dili: İngilizce DOI: 10.18678/dtfd.864114 İndeks Tarihi: 14-06-2021

Assessment of the Immunogenicity and Protective Aspects of a DNA Vaccine Targeting Crimean Congo Hemorrhagic Fever Virus Glycoprotein Gc

Öz:

Aim: Crimean Congo Hemorrhagic Fever (CCHF) is a lethal, endemic infectious disease inhuman. For the preventive measures of the disease, there is currently no safe and efficient vaccine,widely for human use. Vaccine development for CCHF virus is an actively researched subject. Inthis study, we aimed to investigate the immunizing and protective potentials of the CCHF virussurface glycoprotein Gc that is delivered as a single antigen via a DNA based vaccine vector.Material and Methods: A DNA based vaccine targeting the immunogenic envelope glycoproteinGc of a CCHF virus isolate with Turkey origin (Ank2) was generated and its immunogenicity andprotective capability against lethal challenge in IFNα/βR-/- receptor knock out mice was assessed.Results: The developed vaccine candidate (pGc) elicited a considerable amount of neutralizingantibody responses in the vaccinated mice. The vaccine candidate significantly induced bothantiviral Th1 and B cell activating Th2 immune responses deduced from the cytokineproduction profiles in the vaccinated mice. However, despite the immune responses elicitedpost-immunization, the vaccine failed to confer protection against lethal CCHF virus infection.Conclusion: To the best of our knowledge, this is the first report of a DNA vaccine candidategenerated against CCHF virus based on the glycoprotein Gc. The pGc vaccine candidate exhibitedantigen-specific immunity in IFN/α/βR-/- mice, but was unable to produce a protection upon lethalchallenge with the homologous CCHF virus. Once we comprehensively understand the immunecorrelates of protection, we will be more eligible to significantly improve the efficacy of vaccines.

Anahtar Kelime:

Kırım Kongo Kanamalı Ateşi Virüsü Glikoprotein Gc’yi Hedef Alan Bir DNA Aşısının Bağışıklık ve Koruyuculuk Sağlama Özelliklerinin Değerlendirilmesi

Öz:

Amaç: Kırım Kongo Kanamalı Ateşi (KKKA), insanda ölümcül, endemik bir enfeksiyon hastalığıdır. Hastalığın önleyici tedbirleri için şu anda insanlarda yaygın olarak kullanılmak üzere güvenli ve etkili bir aşı bulunmamaktadır. KKKA virüsü için aşı geliştirilmesi, aktif olarak araştırılan bir konudur. Bu çalışmada, DNA esaslı bir aşı vektörü ile tek bir antijen olarak verilen KKKA virüsü yüzey glikoproteini Gc'nin bağışıklık kazandırıcı ve koruyucu potansiyellerinin araştırılması amaçlanmıştır. Gereç ve Yöntemler: Türkiye menşeli (Ank2) bir KKKA virüs izolatının immünojenik özellikteki zarf glikoproteini Gc'yi hedefleyen DNA esaslı bir aşı oluşturulmuş ve bu aşı adayının immünojenisitesi ve aşılanmış IFNα/βR-/- farelerde öldürücü doza karşı koruyucu yeteneği değerlendirilmiştir. Bulgular: Geliştirilen aşı adayı (pGc), aşılanmış farelerde önemli miktarda nötralize edici antikor yanıtı ortaya çıkardı. Aşı adayı, aşılanmış farelerde hem antiviral Th1, hem de B hücresini aktive eden Th2 bağışıklık tepkilerini önemli ölçüde uyardı. Bununla birlikte, aşılama sonrasında ortaya çıkan bağışıklık yanıtlarına rağmen, aşı, ölümcül KKKA virüsü enfeksiyonuna karşı koruma sağlayamadı. Sonuç: Bildiğimiz kadarıyla bu çalışma, glikoprotein Gc'yi hedef alan KKKA virüsüne karşı oluşturulan bir DNA aşı adayının ilk raporudur. PGc aşı adayı, IFNα/βR-/- farelerde antijene özgü bağışıklık yanıtı oluşturdu, ancak ölümcül dozdaki homolog KKKA virüsüne karşı bir koruma üretemedi. Aşı aracılı korunmanın bağışıklık ile olan ilişkilerini daha detaylı olarak anladığımızda, aşıların etkinliğini önemli ölçüde iyileştirme kabiliyetine sahip olacağız.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Sahin E, Coleri Cihan A, A.Farzani T, Földes K, Engin D, Ozkul A (2021). Assessment of the Immunogenicity and Protective Aspects of a DNA Vaccine Targeting Crimean Congo Hemorrhagic Fever Virus Glycoprotein Gc. , 66 - 75. 10.18678/dtfd.864114
Chicago	Sahin Ergin,Coleri Cihan Arzu,A.Farzani Touraj,Földes Katalin,Engin Doruk,Ozkul Aykut Assessment of the Immunogenicity and Protective Aspects of a DNA Vaccine Targeting Crimean Congo Hemorrhagic Fever Virus Glycoprotein Gc. (2021): 66 - 75. 10.18678/dtfd.864114
MLA	Sahin Ergin,Coleri Cihan Arzu,A.Farzani Touraj,Földes Katalin,Engin Doruk,Ozkul Aykut Assessment of the Immunogenicity and Protective Aspects of a DNA Vaccine Targeting Crimean Congo Hemorrhagic Fever Virus Glycoprotein Gc. , 2021, ss.66 - 75. 10.18678/dtfd.864114
AMA	Sahin E,Coleri Cihan A,A.Farzani T,Földes K,Engin D,Ozkul A Assessment of the Immunogenicity and Protective Aspects of a DNA Vaccine Targeting Crimean Congo Hemorrhagic Fever Virus Glycoprotein Gc. . 2021; 66 - 75. 10.18678/dtfd.864114
Vancouver	Sahin E,Coleri Cihan A,A.Farzani T,Földes K,Engin D,Ozkul A Assessment of the Immunogenicity and Protective Aspects of a DNA Vaccine Targeting Crimean Congo Hemorrhagic Fever Virus Glycoprotein Gc. . 2021; 66 - 75. 10.18678/dtfd.864114
IEEE	Sahin E,Coleri Cihan A,A.Farzani T,Földes K,Engin D,Ozkul A "Assessment of the Immunogenicity and Protective Aspects of a DNA Vaccine Targeting Crimean Congo Hemorrhagic Fever Virus Glycoprotein Gc." , ss.66 - 75, 2021. 10.18678/dtfd.864114
ISNAD	Sahin, Ergin vd. "Assessment of the Immunogenicity and Protective Aspects of a DNA Vaccine Targeting Crimean Congo Hemorrhagic Fever Virus Glycoprotein Gc". (2021), 66-75. https://doi.org/10.18678/dtfd.864114

APA	Sahin E, Coleri Cihan A, A.Farzani T, Földes K, Engin D, Ozkul A (2021). Assessment of the Immunogenicity and Protective Aspects of a DNA Vaccine Targeting Crimean Congo Hemorrhagic Fever Virus Glycoprotein Gc. Düzce Tıp Fakültesi Dergisi, 23(1), 66 - 75. 10.18678/dtfd.864114
Chicago	Sahin Ergin,Coleri Cihan Arzu,A.Farzani Touraj,Földes Katalin,Engin Doruk,Ozkul Aykut Assessment of the Immunogenicity and Protective Aspects of a DNA Vaccine Targeting Crimean Congo Hemorrhagic Fever Virus Glycoprotein Gc. Düzce Tıp Fakültesi Dergisi 23, no.1 (2021): 66 - 75. 10.18678/dtfd.864114
MLA	Sahin Ergin,Coleri Cihan Arzu,A.Farzani Touraj,Földes Katalin,Engin Doruk,Ozkul Aykut Assessment of the Immunogenicity and Protective Aspects of a DNA Vaccine Targeting Crimean Congo Hemorrhagic Fever Virus Glycoprotein Gc. Düzce Tıp Fakültesi Dergisi, vol.23, no.1, 2021, ss.66 - 75. 10.18678/dtfd.864114
AMA	Sahin E,Coleri Cihan A,A.Farzani T,Földes K,Engin D,Ozkul A Assessment of the Immunogenicity and Protective Aspects of a DNA Vaccine Targeting Crimean Congo Hemorrhagic Fever Virus Glycoprotein Gc. Düzce Tıp Fakültesi Dergisi. 2021; 23(1): 66 - 75. 10.18678/dtfd.864114
Vancouver	Sahin E,Coleri Cihan A,A.Farzani T,Földes K,Engin D,Ozkul A Assessment of the Immunogenicity and Protective Aspects of a DNA Vaccine Targeting Crimean Congo Hemorrhagic Fever Virus Glycoprotein Gc. Düzce Tıp Fakültesi Dergisi. 2021; 23(1): 66 - 75. 10.18678/dtfd.864114
IEEE	Sahin E,Coleri Cihan A,A.Farzani T,Földes K,Engin D,Ozkul A "Assessment of the Immunogenicity and Protective Aspects of a DNA Vaccine Targeting Crimean Congo Hemorrhagic Fever Virus Glycoprotein Gc." Düzce Tıp Fakültesi Dergisi, 23, ss.66 - 75, 2021. 10.18678/dtfd.864114
ISNAD	Sahin, Ergin vd. "Assessment of the Immunogenicity and Protective Aspects of a DNA Vaccine Targeting Crimean Congo Hemorrhagic Fever Virus Glycoprotein Gc". Düzce Tıp Fakültesi Dergisi 23/1 (2021), 66-75. https://doi.org/10.18678/dtfd.864114