The Effect of Iodised Poly-3-Hydroxy Butyrate on DNA and BSA
Yıl: 2021 Cilt: 9 Sayı: 2 Sayfa Aralığı: 134 - 139 Metin Dili: İngilizce DOI: 10.14235/bas.galenos.2020.4056 İndeks Tarihi: 14-06-2021
The Effect of Iodised Poly-3-Hydroxy Butyrate on DNA and BSA
Öz: Objective: An important criterion that radiopaque poly-3-hydroxybutyrate (PHB) derivatives synthesized and characterized by ourstudy group do not interact with DNA and bovine albumin to bedeveloped as an intravenous contrast agent for radiological imaging.Methods: Interactions of radiopaque PHB derivatives betweenbovine serum albumin (BSA) and deoxyribonucleic acid (pUC19DNA and polymerase chain reaction product) were investigatedusing absorption spectroscopy and agarose gel electrophoresistechniques.Results: Absorption spectroscopy results showed that the radiopaquePHB derivatives did not show any interaction with BSA. Doublestranded annular DNA showed that the interaction between thedouble-stranded forms of linear DNA did not cause any damage.Conclusion: It was determined that it did not interact with bothBSA and various DNA forms.
Anahtar Kelime: İyotlu Poli-3-Hidroksi Butiratın DNA ve BSA Üzerine Etkisi
Öz: Amaç: Çalışma grubumuz tarafından sentezi ve karakterizasyonu yapılan radyopak poly-3-hydroxy butyrate (PHB) türevlerinin, radyolojik görüntüleme kullanılacak, intravenöz kontrast madde olarak geliştirilebilmesi için, DNA ve sığır albumin ile etkileşim göstermemesi önemli bir kriterdir. Yöntemler: Radyoopak PHB türevlerinin sığır serum albumin (BSA) ve deoksiribonükleik asit (pUC19 DNA’sı ve polimeraz zincir reaksiyonu ürünü) arasındaki etkileşimleri absorpsiyon spektroskopisi ve agaroz jel elektroforezi teknikleri kullanılarak incelenmiştir. Bulgular: Absorpsiyon spektroskopisi sonuçları, radyoopak PHB türevlerinin BSA ile bir etkileşim göstermediği, absorpsiyon spektroskopisi ve agaroz jel elektroforezi sonuçları ise; çift iplikli halkasal DNA, çift iplikli lineer DNA formları arasın bir etkileşim ve hasara neden olmadığını göstermiştir. Sonuç: Hem BSA hem de çeşitli DNA formları ile etkileşimi olmadığı tespit edilmiştir
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | EROL A (2021). The Effect of Iodised Poly-3-Hydroxy Butyrate on DNA and BSA. , 134 - 139. 10.14235/bas.galenos.2020.4056 |
| Chicago | EROL ARZU The Effect of Iodised Poly-3-Hydroxy Butyrate on DNA and BSA. (2021): 134 - 139. 10.14235/bas.galenos.2020.4056 |
| MLA | EROL ARZU The Effect of Iodised Poly-3-Hydroxy Butyrate on DNA and BSA. , 2021, ss.134 - 139. 10.14235/bas.galenos.2020.4056 |
| AMA | EROL A The Effect of Iodised Poly-3-Hydroxy Butyrate on DNA and BSA. . 2021; 134 - 139. 10.14235/bas.galenos.2020.4056 |
| Vancouver | EROL A The Effect of Iodised Poly-3-Hydroxy Butyrate on DNA and BSA. . 2021; 134 - 139. 10.14235/bas.galenos.2020.4056 |
| IEEE | EROL A "The Effect of Iodised Poly-3-Hydroxy Butyrate on DNA and BSA." , ss.134 - 139, 2021. 10.14235/bas.galenos.2020.4056 |
| ISNAD | EROL, ARZU. "The Effect of Iodised Poly-3-Hydroxy Butyrate on DNA and BSA". (2021), 134-139. https://doi.org/10.14235/bas.galenos.2020.4056 |
| APA | EROL A (2021). The Effect of Iodised Poly-3-Hydroxy Butyrate on DNA and BSA. Bezmiâlem Science, 9(2), 134 - 139. 10.14235/bas.galenos.2020.4056 |
| Chicago | EROL ARZU The Effect of Iodised Poly-3-Hydroxy Butyrate on DNA and BSA. Bezmiâlem Science 9, no.2 (2021): 134 - 139. 10.14235/bas.galenos.2020.4056 |
| MLA | EROL ARZU The Effect of Iodised Poly-3-Hydroxy Butyrate on DNA and BSA. Bezmiâlem Science, vol.9, no.2, 2021, ss.134 - 139. 10.14235/bas.galenos.2020.4056 |
| AMA | EROL A The Effect of Iodised Poly-3-Hydroxy Butyrate on DNA and BSA. Bezmiâlem Science. 2021; 9(2): 134 - 139. 10.14235/bas.galenos.2020.4056 |
| Vancouver | EROL A The Effect of Iodised Poly-3-Hydroxy Butyrate on DNA and BSA. Bezmiâlem Science. 2021; 9(2): 134 - 139. 10.14235/bas.galenos.2020.4056 |
| IEEE | EROL A "The Effect of Iodised Poly-3-Hydroxy Butyrate on DNA and BSA." Bezmiâlem Science, 9, ss.134 - 139, 2021. 10.14235/bas.galenos.2020.4056 |
| ISNAD | EROL, ARZU. "The Effect of Iodised Poly-3-Hydroxy Butyrate on DNA and BSA". Bezmiâlem Science 9/2 (2021), 134-139. https://doi.org/10.14235/bas.galenos.2020.4056 |
