Yıl: 2021 Cilt: 7 Sayı: 2 Sayfa Aralığı: 206 - 211 Metin Dili: İngilizce DOI: 10.53394/akd.959254 İndeks Tarihi: 29-07-2022

Brusatol Mitigates Ovarian Tissue Oxidatif Injury Induced by Ovarian Ischemia Reperfusion

Öz:
Objective: In the scope of this study, it was tried to examine the probable benefits of brusatol againstovarian tissue injury originating from bilateral ovarian torsion/detorsion (T/D).Material and Methods: In this study, experimental animals were randomized to 4 groups. Groupswere programmed as sham group I (control), group II (T/D), group III (DMSO+T/D) and groupIV (Brusatol+T/D). Group I; the abdomen region was barbered, disinfected, opened by incision andclosed respectively, but no T/D model was performed. Group II, as described in group I, after openingand closing abdominal incision, 3 hours of torsion was applied and it was followed by a 3-hour ofdetorsion. Group III, 1% DMSO was applied intraperitoneally 0,3 ml as every other day for 10days and the last dose was given 30 minutes prior to detorsion. Group IV; brusatol was administeredintraperitoneally 0,5 mg/ml once every 2 days for 10 days and the last dose was applied 30 minutesprior to detorsion. Then, all processes were carried out as described in group II. Following detorsionperiod, in pursuit of sacrificing the rats, ovarian tissues were removed.Results: Oxidant parameters (MDA, MPO, TOS, OSI) and pro-inflammatory cytokine (TNF-α,IL-1β) levels increased significantly while antioxidant levels (SOD, TAS) reduced in group II and IIIcompared to group I (p<0.05). On the contrary, brusatol application (group IV) reversed all of thesevalues compared to group II and III (p<0.05).Conclusion: As a conclusion, brusatol was evaluated to have protective effects against T/D-inducedovarian injury in rats.
Anahtar Kelime: Brusatol Rat Torsion-Detorsion Ischemia Reperfusion Ovary

Brusatol Over İskemi Reperfüzyonu ile İndüklenmiş Over Dokusu Oksidatif Yaralanmasını Azaltır

Öz:
Amaç: Bu çalışmanın kapsamında bilateral over torsiyon/detorsiyonundan (T/D) kaynaklanan over dokusu yaralanmasına karşı brusatolün olası yararları araştırılmaya çalışıldı. Gereç ve Yöntemler: Bu çalışmada deney hayvanları 4 gruba randomize edildi. Gruplar grup I (sham-kontrol), grup II (T/D), grup III (DMSO+T/D) ve grup IV (Brusatol+T/D) olarak programlandı. Grup I; karın bölgesi traşlanıp, dezenfekte edildi, kesi ile açıldı ve tekrar kapatıldı, ancak T/D modeli uygulanmadı. Grup II'de, grup I'de tarif edildiği gibi, abdominal insizyon yapıldıktan sonra 3 saat torsiyon uygulandı ve bunu 3 saatlik detorsiyon izledi. Grup III, %1 DMSO, 10 gün boyunca her gün olduğu gibi intraperitonal olarak 0,3 ml uygulandı ve son doz detorsiyondan 30 dakika önce uygulandı. Daha sonra, tüm işlemler grup II' de tarif edildiği gibi gerçekleştirildi. Grup IV; brusatol, 10 gün boyunca her 2 günde bir 0,5 mg/ml intraperitonal olarak uygulandı ve detorsiyondan 30 dakika önce son dozu verildi. Detorsiyon süresinin ardından sıçanların sakrifiye edilerek over dokuları çıkarıldı. Bulgular: Grup II ve III' te grup I ile karşılaştırıldığında, oksidan parametreler (MDA, MPO, TOS, OSI) ve proinflamatuar sitokin (TNF-α, IL-1β) düzeyleri artarken, antioksidan (SOD, TAS) parametreler önemli ölçüde azaldı (p<0.05). Aksine, brusatol uygulaması (grup IV), grup II ve III' e kıyasla bu değerlerin hepsini tersine çevirdi (p<0.05). Sonuç: Sonuç olarak, brusatol’ ün sıçanlarda T/D kaynaklı over hasarına karşı koruyucu etkileri olduğu değerlendirildi.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Ekinci Akdemir F, tanyeli a, guler m, Eraslan E, TOPDAĞI YILMAZ E, TOPDAĞI Y (2021). Brusatol Mitigates Ovarian Tissue Oxidatif Injury Induced by Ovarian Ischemia Reperfusion. , 206 - 211. 10.53394/akd.959254
Chicago Ekinci Akdemir Fazile Nur,tanyeli ayhan,guler mustafa can,Eraslan Ersen,TOPDAĞI YILMAZ Emsal Pınar,TOPDAĞI YUNUS EMRE Brusatol Mitigates Ovarian Tissue Oxidatif Injury Induced by Ovarian Ischemia Reperfusion. (2021): 206 - 211. 10.53394/akd.959254
MLA Ekinci Akdemir Fazile Nur,tanyeli ayhan,guler mustafa can,Eraslan Ersen,TOPDAĞI YILMAZ Emsal Pınar,TOPDAĞI YUNUS EMRE Brusatol Mitigates Ovarian Tissue Oxidatif Injury Induced by Ovarian Ischemia Reperfusion. , 2021, ss.206 - 211. 10.53394/akd.959254
AMA Ekinci Akdemir F,tanyeli a,guler m,Eraslan E,TOPDAĞI YILMAZ E,TOPDAĞI Y Brusatol Mitigates Ovarian Tissue Oxidatif Injury Induced by Ovarian Ischemia Reperfusion. . 2021; 206 - 211. 10.53394/akd.959254
Vancouver Ekinci Akdemir F,tanyeli a,guler m,Eraslan E,TOPDAĞI YILMAZ E,TOPDAĞI Y Brusatol Mitigates Ovarian Tissue Oxidatif Injury Induced by Ovarian Ischemia Reperfusion. . 2021; 206 - 211. 10.53394/akd.959254
IEEE Ekinci Akdemir F,tanyeli a,guler m,Eraslan E,TOPDAĞI YILMAZ E,TOPDAĞI Y "Brusatol Mitigates Ovarian Tissue Oxidatif Injury Induced by Ovarian Ischemia Reperfusion." , ss.206 - 211, 2021. 10.53394/akd.959254
ISNAD Ekinci Akdemir, Fazile Nur vd. "Brusatol Mitigates Ovarian Tissue Oxidatif Injury Induced by Ovarian Ischemia Reperfusion". (2021), 206-211. https://doi.org/10.53394/akd.959254
APA Ekinci Akdemir F, tanyeli a, guler m, Eraslan E, TOPDAĞI YILMAZ E, TOPDAĞI Y (2021). Brusatol Mitigates Ovarian Tissue Oxidatif Injury Induced by Ovarian Ischemia Reperfusion. Akdeniz Tıp Dergisi, 7(2), 206 - 211. 10.53394/akd.959254
Chicago Ekinci Akdemir Fazile Nur,tanyeli ayhan,guler mustafa can,Eraslan Ersen,TOPDAĞI YILMAZ Emsal Pınar,TOPDAĞI YUNUS EMRE Brusatol Mitigates Ovarian Tissue Oxidatif Injury Induced by Ovarian Ischemia Reperfusion. Akdeniz Tıp Dergisi 7, no.2 (2021): 206 - 211. 10.53394/akd.959254
MLA Ekinci Akdemir Fazile Nur,tanyeli ayhan,guler mustafa can,Eraslan Ersen,TOPDAĞI YILMAZ Emsal Pınar,TOPDAĞI YUNUS EMRE Brusatol Mitigates Ovarian Tissue Oxidatif Injury Induced by Ovarian Ischemia Reperfusion. Akdeniz Tıp Dergisi, vol.7, no.2, 2021, ss.206 - 211. 10.53394/akd.959254
AMA Ekinci Akdemir F,tanyeli a,guler m,Eraslan E,TOPDAĞI YILMAZ E,TOPDAĞI Y Brusatol Mitigates Ovarian Tissue Oxidatif Injury Induced by Ovarian Ischemia Reperfusion. Akdeniz Tıp Dergisi. 2021; 7(2): 206 - 211. 10.53394/akd.959254
Vancouver Ekinci Akdemir F,tanyeli a,guler m,Eraslan E,TOPDAĞI YILMAZ E,TOPDAĞI Y Brusatol Mitigates Ovarian Tissue Oxidatif Injury Induced by Ovarian Ischemia Reperfusion. Akdeniz Tıp Dergisi. 2021; 7(2): 206 - 211. 10.53394/akd.959254
IEEE Ekinci Akdemir F,tanyeli a,guler m,Eraslan E,TOPDAĞI YILMAZ E,TOPDAĞI Y "Brusatol Mitigates Ovarian Tissue Oxidatif Injury Induced by Ovarian Ischemia Reperfusion." Akdeniz Tıp Dergisi, 7, ss.206 - 211, 2021. 10.53394/akd.959254
ISNAD Ekinci Akdemir, Fazile Nur vd. "Brusatol Mitigates Ovarian Tissue Oxidatif Injury Induced by Ovarian Ischemia Reperfusion". Akdeniz Tıp Dergisi 7/2 (2021), 206-211. https://doi.org/10.53394/akd.959254