Objective: Sjogren's syndrome is an autoimmune inflammatory disease characterized by lymphocytic infiltration of the exocrine glands. There are various classification criteria for Sjögren's syndrome which include various serological tests, symptoms, and histopathological findings. In this study, it was aimed to evaluate the relationship between minor salivary gland biopsy results and serological features. Material and Methods: The relationship between minor salivary gland biopsy and anti-nuclear antibody, extracted nuclear antigen, and the dense specled antibody results were evaluated. P <0.05 was considered significant. Results: 77 patients (71F, 6M) were included in the study. In 22.1% of the patients the anti-nuclear antibody was in a fine speckled pattern. The 31.2% of the patients was histopathologically compatible with Sjögren syndrome (focus score≥1). Anti dense fine speckled 70 antibody was positive in 19.5% of the patients and anti SS-A antibody in 26%. Seven of the 15 patients with anti dense fine speckled 70 antibody positivity were diagnosed with Sjögren's syndrome. There was no difference between anti dense fine speckled 70 antibody positive and negative patients in terms of diagnosis of Sjögren's syndrome and biopsy positivity (p = 0.905, p = 0.996). Conclusion: Minor salivary gland biopsy has very important place in diagnosis of Sjögren syndrome. Minor salivary gland biopsy gives the chance to diagnose Sjögren's syndrome in patients with negative anti nuclear antibodies, anti SS-A and anti SS-B antibodies. İsolated anti dense fine speckled 70 antibody positivity does not exclude Sjögren syndrome.