Yıl: 2021 Cilt: 5 Sayı: 1 Sayfa Aralığı: 51 - 57 Metin Dili: İngilizce DOI: 10.30616/ajb.893690 İndeks Tarihi: 29-07-2022

Effects of resveratrol and 1,3-bis(2-chloroethyl)-1-nitrosurea combination on YKG1 glioblastoma cells

Öz:
Glioblastoma is a primary malignant brain tumor that can be treated with 1,3-bis(2-chloroethyl)-1-nitrosurea(BiCNU/carmustine). Resveratrol is a natural phenol that can interfere with apoptosis. This study aims to investigate how thecombination of BiCNU and resveratrol affects glioblastoma cells in vitro. Accordingly, YKG1 glioblastoma cells were treatedwith different amounts of resveratrol (50 and 100 µM) and BiCNU (10 and 20 µM) either alone or in combination. Cellviability tests and immunochemical studies were conducted on these cells. According to results, increasing the amount ofresveratrol and BiCNU decreased cell viability. Additionally, when these maximal doses of resveratrol and BiCNU (100 µMresveratrol plus 20 µM BiCNU) were applied, viability decreased to the highest cytotoxicity levels. Immunohistochemicalanalysis also revealed the significantly upregulated H scores of beclin-1 and caspase-3 in treated groups with the highest valuein maximally combined concentration. These results indicated the cumulative effects of concurrent administration of BiCNUand resveratrol on the cytotoxicity of malignant human YKG1 glioblastoma cells in vitro.
Anahtar Kelime: Apoptosis Carmustine Glioblastoma Resveratrol

Resveratrol ve 1,3-bis(2-kloroetil)-1-nitrozüre kombinasyonunun YKG1 glioblastoma hücreleri üzerine etkileri

Öz:
Glioblastoma, 1,3-bis (2-kloroetil) -1-nitrosüre (BiCNU / carmustin) ile tedavi edilebilen birincil kötü huylu beyin tümörüdür. Resveratrol, apoptozu engelleyebilen doğal bir fenoldür. Bu çalışma, BiCNU ve resveratrol kombinasyonunun glioblastoma hücrelerini in vitro nasıl etkilediğini araştırmayı amaçlamaktadır. Çalışma kapsamında glioblastoma YKG1 hücreleri, tek başına veya kombinasyon halinde farklı miktarlarda resveratrol (50 ve 100 uM) ve BiCNU (10 ve 20 uM) ile muamele edildi. Bu hücreler üzerinde hücre canlılığı testleri ve immünokimyasal çalışmalar yapıldı. Sonuçlara göre, resveratrol ve BiCNU miktarının artırılması hücre canlılığını kademeli olarak azalttı. Ek olarak, maksimum resveratrol ve BiCNU dozu (100 µM resveratrol artı 20 µM BiCNU) verildiğinde, hücre canlılıkları oldukça azaldı. İmmünohistokimyasal analizler, maksimum kombine konsantrasyonda muamele edilen gruplarda beklin-1 ve kaspaz-3 H skorları düzeylerini önemli ölçüde arttırdığını ortaya çıkarmıştır. Bu sonuçlar, BiCNU ve resveratrolün aynı anda uygulanmasının, malign insan YKG1 glioblastoma hücrelerinin in vitro sitotoksisitesi üzerindeki kümülatif etkilerini göstermiştir.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA pektas g, Aslan E, GüZel H, DEMİRCİLER YAVAŞ B, ÇELİK S (2021). Effects of resveratrol and 1,3-bis(2-chloroethyl)-1-nitrosurea combination on YKG1 glioblastoma cells. , 51 - 57. 10.30616/ajb.893690
Chicago pektas gokhan,Aslan Esra,GüZel Hilal,DEMİRCİLER YAVAŞ BETÜL,ÇELİK Sefa Effects of resveratrol and 1,3-bis(2-chloroethyl)-1-nitrosurea combination on YKG1 glioblastoma cells. (2021): 51 - 57. 10.30616/ajb.893690
MLA pektas gokhan,Aslan Esra,GüZel Hilal,DEMİRCİLER YAVAŞ BETÜL,ÇELİK Sefa Effects of resveratrol and 1,3-bis(2-chloroethyl)-1-nitrosurea combination on YKG1 glioblastoma cells. , 2021, ss.51 - 57. 10.30616/ajb.893690
AMA pektas g,Aslan E,GüZel H,DEMİRCİLER YAVAŞ B,ÇELİK S Effects of resveratrol and 1,3-bis(2-chloroethyl)-1-nitrosurea combination on YKG1 glioblastoma cells. . 2021; 51 - 57. 10.30616/ajb.893690
Vancouver pektas g,Aslan E,GüZel H,DEMİRCİLER YAVAŞ B,ÇELİK S Effects of resveratrol and 1,3-bis(2-chloroethyl)-1-nitrosurea combination on YKG1 glioblastoma cells. . 2021; 51 - 57. 10.30616/ajb.893690
IEEE pektas g,Aslan E,GüZel H,DEMİRCİLER YAVAŞ B,ÇELİK S "Effects of resveratrol and 1,3-bis(2-chloroethyl)-1-nitrosurea combination on YKG1 glioblastoma cells." , ss.51 - 57, 2021. 10.30616/ajb.893690
ISNAD pektas, gokhan vd. "Effects of resveratrol and 1,3-bis(2-chloroethyl)-1-nitrosurea combination on YKG1 glioblastoma cells". (2021), 51-57. https://doi.org/10.30616/ajb.893690
APA pektas g, Aslan E, GüZel H, DEMİRCİLER YAVAŞ B, ÇELİK S (2021). Effects of resveratrol and 1,3-bis(2-chloroethyl)-1-nitrosurea combination on YKG1 glioblastoma cells. Anatolian Journal of Botany, 5(1), 51 - 57. 10.30616/ajb.893690
Chicago pektas gokhan,Aslan Esra,GüZel Hilal,DEMİRCİLER YAVAŞ BETÜL,ÇELİK Sefa Effects of resveratrol and 1,3-bis(2-chloroethyl)-1-nitrosurea combination on YKG1 glioblastoma cells. Anatolian Journal of Botany 5, no.1 (2021): 51 - 57. 10.30616/ajb.893690
MLA pektas gokhan,Aslan Esra,GüZel Hilal,DEMİRCİLER YAVAŞ BETÜL,ÇELİK Sefa Effects of resveratrol and 1,3-bis(2-chloroethyl)-1-nitrosurea combination on YKG1 glioblastoma cells. Anatolian Journal of Botany, vol.5, no.1, 2021, ss.51 - 57. 10.30616/ajb.893690
AMA pektas g,Aslan E,GüZel H,DEMİRCİLER YAVAŞ B,ÇELİK S Effects of resveratrol and 1,3-bis(2-chloroethyl)-1-nitrosurea combination on YKG1 glioblastoma cells. Anatolian Journal of Botany. 2021; 5(1): 51 - 57. 10.30616/ajb.893690
Vancouver pektas g,Aslan E,GüZel H,DEMİRCİLER YAVAŞ B,ÇELİK S Effects of resveratrol and 1,3-bis(2-chloroethyl)-1-nitrosurea combination on YKG1 glioblastoma cells. Anatolian Journal of Botany. 2021; 5(1): 51 - 57. 10.30616/ajb.893690
IEEE pektas g,Aslan E,GüZel H,DEMİRCİLER YAVAŞ B,ÇELİK S "Effects of resveratrol and 1,3-bis(2-chloroethyl)-1-nitrosurea combination on YKG1 glioblastoma cells." Anatolian Journal of Botany, 5, ss.51 - 57, 2021. 10.30616/ajb.893690
ISNAD pektas, gokhan vd. "Effects of resveratrol and 1,3-bis(2-chloroethyl)-1-nitrosurea combination on YKG1 glioblastoma cells". Anatolian Journal of Botany 5/1 (2021), 51-57. https://doi.org/10.30616/ajb.893690