Oğuz AĞYAR
(Adıyaman Üniversitesi, Kahta Meslek Yüksek Okulu, Veterinerlik Bölümü, Adıyaman, 02440, Türkiye)
Emin ÖZKÖSE
(Kahramanmaraş Sütçü İmam Üniversitesi, Ziraat Fakültesi, Hayvan Bilimleri Bölümü, Kahramanmaraş, Türkiye)
M. Sait EKİNCİ
(Kahramanmaraş Sütçü İmam Üniversitesi, Ziraat Fakültesi, Hayvan Bilimleri Bölümü, Kahramanmaraş, Türkiye)
İsmail AKYOL
(Ankara Üniversitesi, Ziraat Fakültesi, Tarımsal Biyoteknoloji Bölümü, Ankara, Türkiye)
Yıl: 2021Cilt: 8Sayı: 1ISSN: 2148-3647 / 2148-3647Sayfa Aralığı: 171 - 178İngilizce

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Molecular Phylogenetic Analysis of Morkaraman Sheep in Bingol Region
In the present study, we report mitochondrial DNA (mtDNA) 16S rRNA gene sequence analysis of theMorkaraman ewe, (Ovis aries) was investigated. In domestic sheep, the 16S rRNA gene is ca 1574 bp in length.Partial fragment of 536 bp 16S rRNA gene of mtDNA was amplified by using designed primers from publisheddata of Sheep sequences derived from National Center for Biotechnology Information (NCBI) web server(ncbi.nlm.nih.gov/NC_001941.1). Homology of the sequences obtained from the present work were performedby using BLAST program at NCBI. Obtained 16S rRNA gene sequences were submited to the GenBank databasewith accession numbers, KU686952.1, KU686953.1, KU686954.1, KU686955.1, KU686956.1, KU686957.1,KU686958.1, KU686959.1, KU686960.1, KU686961.1 and KU686962.1, respectively. According to nucleotidedifferences of mtDNA 16S rRNA gene region 10 polymorphic regions and 8 haplotypes were detected in Turkishdomestic sheep Morkaraman (n=11). The nucleotide composition averages of all the sequences was 32.1%Adenine (A), 24.2% Thymine (T), 22.2% Cytosine (C) and 21.5% Guanine (G); G+C was 43.7%. DNApolymorphism based on 16S rRNA gene sequences, haplotype diversity (Hd) and nucleotide diversity (π) werefound to be 0,9273±0,00442, and 0,00427±0,00125, respectively. Based on gene sequences information, insheeps, mtDNA haplotypes, the phylogenetic relationship among mtDNA polymorphism, haplotypes andrelationship between native sheep breeds and foreign sheep breeds were determined and discussed. Thus,sequence analysis of mitochondrial 16S rRNA gene can be used as a tool for phylogenetic analysis ofMorkaraman sheep.
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