Ratlarda Kurşun Asetat Kaynaklı Karaciğer Hasarı Üzerine Chrysin’ in Etkilerinin Araştırılması

Pınar COŞKUN, (Bingöl Üniversitesi, Fen Bilimleri Enstitüsü, Bingöl, Türkiye)
Fulya BENZER, (Munzur Üniversitesi, Sağlık Bilimleri Fakültesi, Ebelik Bölümü, Tunceli, Türkiye)
Fatih Mehmet KANDEMİR, (Atatürk Üniversitesi, Veteriner Fakültesi, Biyokimya Bölümü, Erzurum, Türkiye)
Serkan YILDIRIM, (Atatürk Üniversitesi, Veteriner Fakültesi, Patoloji Bölümü, Erzurum, Türkiye)
Sefa KÜÇÜKLER (Atatürk Üniversitesi, Veteriner Fakültesi, Biyokimya Bölümü, Erzurum, Türkiye)
Türk Tarım ve Doğa Bilimleri Dergisi
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Yıl: 2021 Cilt: 8 Sayı: 2 Sayfa Aralığı: 250 - 261 Metin Dili: Türkçe DOI: 10.30910/turkjans.876603 İndeks Tarihi: 28-09-2021

Ratlarda Kurşun Asetat Kaynaklı Karaciğer Hasarı Üzerine Chrysin’ in Etkilerinin Araştırılması

Öz:
Bu çalışma ratlarda kurşun asetat (PbA) kaynaklı karaciğer hasarı üzerine chrysin’in (CH)etkilerinin araştırılması amacıyla yapıldı. Çalışmada 35 adet Sprague Dawley cinsi erkek rat, her grupta 7rat olacak şekilde 5 gruba ayrıldı. 1. Grup (Kontrol): Oral yolla serum fizyolojik verildi. 2. Grup (CH grubu):CH (50 mg kg-1 ağırlık gün-1) 7 gün boyunca oral olarak ratlara verildi. 3. Grup (PbA grubu): PbA (30 mgkg-1) 7 gün boyunca 30 mg kg-1 PbA oral yolla ratlara verildi. 4. Grup (PbA+CH 25 grubu): 7 gün boyunca25 mg/kg CH uygulamasından 30 dakika sonra 30 mg kg-1 PbA ratlara oral yoldan verildi. 5. Grup (PbA+CH 50 grubu): 7 gün süreyle 50 mg/kg CH uygulamasından 30 dakika sonra 30 mg kg-1 PbA ratlara oralyoldan verildi. Son uygulamadan 24 saat sonra (8.günde) ratlar hafif sevofloran anestezisi altındaötenazileri yapılarak, doku ve kan örnekleri alındı. Serumda AST, ALT ve ALP ile karaciğer dokusundaMDA, GSH ve NO düzeyleri ile SOD, KAT, GPx ve arginaz enzim aktivitelerine bakıldı. Kontrol ilekıyaslandığı zaman serum ALT, AST ve ALP düzeyleri PbA verilen grupta arttı, bu artış CH ile önemliölçüde düzeltildi. Karaciğer MDA ve NO düzeyleri PbA verilen gruplarda artarken, antioksidan enzimaktiviteleri (SOD, KAT ve GPx) ile GSH düzeyi azaldı. CH uygulaması artan MDA ve NO düzeyleriniazaltırken, antioksidan enzim aktiviteleri ile GSH düzeylerini anlamlı olarak artırdı. Histolojik olarak, PbAuygulanan ratların karaciğerlerinde görülen nekroz, hidropik dejenerasyonlar ve mononükleer hücreinfiltrasyonlarını CH’ın azalttığı belirlendi. Sonuç olarak PbA kaynaklı karaciğer hasarlarını azaltmada CHetkili olmuştur.
Anahtar Kelime:

Investigation of Chrysin Effects on Lead Asetat-Induced Liver Damage in Rats

Öz:
This study was conducted to investigate the effects of chrysin (CH) on lead acetate (PbA) inducing liver injury. In this study, 35 male Sprague Dawley rats were divided into 5 groups as 7 rats in each group. Group 1 (Control): Saline was given orally. Group 2 (CH group): CH (50 mg kg-1 weight day-1 ) were orally given rats for 7 days. Group 3 (PbA group): PbA (30 mg kg-1 ) was orally given to rats at 30 mg kg-1 PbA for 7 days. Group 4 (PbA +CH 25 group): 25 mg/kg CH was orally given to rats for 7 days. In addition, 30 mg kg-1 PbA was given orally to rats for 7 days after 30 minutes of the application of the CH. Group 5 (PbA+CH 50 group): 50 mg kg-1 CH was orally given to rats for 7 days. Furthermore, 30 mg kg-1 PbA was given orally to rats for 7 days after 30 minutes of the application of the CH. The rats were euthanized under mild sevoflurane anesthesia and tissue and blood samples were taken 24 hours after the last application (8th day). AST, ALT and ALP in serum, MDA, GSH and NO levels and SOD, CAT, GPx and arginase enzyme activities in liver tissue were measured. Serum ALT, AST and ALP levels increased in the PbA group when compared with the control, and this increase was partially corrected with CH. Liver MDA and NO levels increased in the PbA administered groups while antioxidant enzyme activities and GSH levels decreased. Although, CH administration decreased the increased MDA and NO levels, it increased the decreased antioxidant enzyme activities and GSH levels. Histologically, it was determined that CH decreased the necrosis, hydropic degenerations and mononuclear cell infiltrations seen in the livers of rats treated with PbA. As a result, CH is effective in the reducing PbA damages in the liver.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA COŞKUN P, BENZER F, KANDEMİR F, yildirim S, KÜÇÜKLER S (2021). Ratlarda Kurşun Asetat Kaynaklı Karaciğer Hasarı Üzerine Chrysin’ in Etkilerinin Araştırılması. , 250 - 261. 10.30910/turkjans.876603
Chicago COŞKUN Pınar,BENZER Fulya,KANDEMİR Fatih Mehmet,yildirim Serkan,KÜÇÜKLER Sefa Ratlarda Kurşun Asetat Kaynaklı Karaciğer Hasarı Üzerine Chrysin’ in Etkilerinin Araştırılması. (2021): 250 - 261. 10.30910/turkjans.876603
MLA COŞKUN Pınar,BENZER Fulya,KANDEMİR Fatih Mehmet,yildirim Serkan,KÜÇÜKLER Sefa Ratlarda Kurşun Asetat Kaynaklı Karaciğer Hasarı Üzerine Chrysin’ in Etkilerinin Araştırılması. , 2021, ss.250 - 261. 10.30910/turkjans.876603
AMA COŞKUN P,BENZER F,KANDEMİR F,yildirim S,KÜÇÜKLER S Ratlarda Kurşun Asetat Kaynaklı Karaciğer Hasarı Üzerine Chrysin’ in Etkilerinin Araştırılması. . 2021; 250 - 261. 10.30910/turkjans.876603
Vancouver COŞKUN P,BENZER F,KANDEMİR F,yildirim S,KÜÇÜKLER S Ratlarda Kurşun Asetat Kaynaklı Karaciğer Hasarı Üzerine Chrysin’ in Etkilerinin Araştırılması. . 2021; 250 - 261. 10.30910/turkjans.876603
IEEE COŞKUN P,BENZER F,KANDEMİR F,yildirim S,KÜÇÜKLER S "Ratlarda Kurşun Asetat Kaynaklı Karaciğer Hasarı Üzerine Chrysin’ in Etkilerinin Araştırılması." , ss.250 - 261, 2021. 10.30910/turkjans.876603
ISNAD COŞKUN, Pınar vd. "Ratlarda Kurşun Asetat Kaynaklı Karaciğer Hasarı Üzerine Chrysin’ in Etkilerinin Araştırılması". (2021), 250-261. https://doi.org/10.30910/turkjans.876603
APA COŞKUN P, BENZER F, KANDEMİR F, yildirim S, KÜÇÜKLER S (2021). Ratlarda Kurşun Asetat Kaynaklı Karaciğer Hasarı Üzerine Chrysin’ in Etkilerinin Araştırılması. Türk Tarım ve Doğa Bilimleri Dergisi, 8(2), 250 - 261. 10.30910/turkjans.876603
Chicago COŞKUN Pınar,BENZER Fulya,KANDEMİR Fatih Mehmet,yildirim Serkan,KÜÇÜKLER Sefa Ratlarda Kurşun Asetat Kaynaklı Karaciğer Hasarı Üzerine Chrysin’ in Etkilerinin Araştırılması. Türk Tarım ve Doğa Bilimleri Dergisi 8, no.2 (2021): 250 - 261. 10.30910/turkjans.876603
MLA COŞKUN Pınar,BENZER Fulya,KANDEMİR Fatih Mehmet,yildirim Serkan,KÜÇÜKLER Sefa Ratlarda Kurşun Asetat Kaynaklı Karaciğer Hasarı Üzerine Chrysin’ in Etkilerinin Araştırılması. Türk Tarım ve Doğa Bilimleri Dergisi, vol.8, no.2, 2021, ss.250 - 261. 10.30910/turkjans.876603
AMA COŞKUN P,BENZER F,KANDEMİR F,yildirim S,KÜÇÜKLER S Ratlarda Kurşun Asetat Kaynaklı Karaciğer Hasarı Üzerine Chrysin’ in Etkilerinin Araştırılması. Türk Tarım ve Doğa Bilimleri Dergisi. 2021; 8(2): 250 - 261. 10.30910/turkjans.876603
Vancouver COŞKUN P,BENZER F,KANDEMİR F,yildirim S,KÜÇÜKLER S Ratlarda Kurşun Asetat Kaynaklı Karaciğer Hasarı Üzerine Chrysin’ in Etkilerinin Araştırılması. Türk Tarım ve Doğa Bilimleri Dergisi. 2021; 8(2): 250 - 261. 10.30910/turkjans.876603
IEEE COŞKUN P,BENZER F,KANDEMİR F,yildirim S,KÜÇÜKLER S "Ratlarda Kurşun Asetat Kaynaklı Karaciğer Hasarı Üzerine Chrysin’ in Etkilerinin Araştırılması." Türk Tarım ve Doğa Bilimleri Dergisi, 8, ss.250 - 261, 2021. 10.30910/turkjans.876603
ISNAD COŞKUN, Pınar vd. "Ratlarda Kurşun Asetat Kaynaklı Karaciğer Hasarı Üzerine Chrysin’ in Etkilerinin Araştırılması". Türk Tarım ve Doğa Bilimleri Dergisi 8/2 (2021), 250-261. https://doi.org/10.30910/turkjans.876603
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