Can Thiol/Disulphide Homeostasis Help in The Differential Diagnosis of Appendicitis in Children?

DINDAR BADEM, NERMIN; Dere Gunal, Yasemin; EREL, Ozcan; neselioglu, salim; Demir, Sabri; ÖZMEN, İSMAİL

doi:10.12956/tchd.627936

Can Thiol/Disulphide Homeostasis Help in The Differential Diagnosis of Appendicitis in Children?

Sabri DEMİR, (Kırıkkale Üniversitesi Tıp Fakültesi Çocuk Cerrahisi Anabilim Dalı, Kırıkkale, Türkiye)

Yasemin DERE GÜNAL, (Kırıkkale Üniversitesi, Tıp Fakültesi, Çocuk Cerrahisi Anabilim Dalı, Kırıkkale, Türkiye)

İsmail ÖZMEN, (Kırıkkale Üniversitesi, Tıp Fakültesi, Çocuk Cerrahisi Anabilim Dalı, Kırıkkale, Türkiye)

Nermin DİNDAR BADEM, (Kırıkkale Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Kırıkkale, Türkiye)

Salim NESELİOGLU, (Yıldırım Beyazıt Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Ankara, Türkiye)

Özcan EREL (Yıldırım Beyazıt Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Ankara, Türkiye)

Türkiye Çocuk Hastalıkları Dergisi

0 0

Yıl: 2020 Cilt: 14 Sayı: 3 Sayfa Aralığı: 236 - 243 Metin Dili: İngilizce DOI: 10.12956/tchd.627936 İndeks Tarihi: 27-07-2021

Can Thiol/Disulphide Homeostasis Help in The Differential Diagnosis of Appendicitis in Children?

Öz:

Objective: We aimed to investigate the potential of assessing thiol/disulfide homeostasis as novel oxidative stressmarkers to improve the challenging diagnosis of acute appendicitis in children.Material and Methods: A total of 60 pediatric patients (0-18 years) were enrolled in the study, 30 of which were inthe control group and 30 in the acute appendicitis group. Native thiol, total thiol, dynamic disulfide, dynamic thiol,ischemia modified albümin, albumin, White blood cell, hemoglobin, hematocrit, and platelet counts of both groups weremeasured. The results of both groups were compared using the SPSS (Statistical Package for Social Sciences) version17 (Chicago, USA) program. For all variables, p <0.05 was considered significant.Results: Total thiol (p<0.001), native thiol (p<0.001), and albümin (p<0.001) levels were significantly decreased whiledynamic disulfide, dynamic thiol (p=0.003), and ischemia modified albümin (p<0.001) levels those indicating oxidantside were increased in acute appendicitis group compared to control. White blood cell counts in acute appendicitisgroup were higher (p<0.001), and platelet counts were lower (p=0.03) than the control group.Conclusion: In the differential diagnosis of acute appendicitis, especially in case that are difficult to diagnose, besides aphysical examination, imaging, and current laboratory tests, quantification of thiol/disulfide homeostasis may be helpfulin diagnosing. In addition, evaluating albumin and IMA levels may increase the specificity of the test. This test can bemore helpful in cases diagnosis is difficult such as children small in their ages and mental retardation.

Anahtar Kelime:

Tiyol / Disülfit Dengesinin Değerlendirilmesi Çocuklarda Apandisitin Ayırıcı Tanısında Yardımcı Olabilir mi?

Öz:

Amaç: Çalışmamızın amacı, tanı konulmasında zorlanılan çocuklarda, akut apandisitin ayırıcı tanısında, oksidatif stresin yeni bir belirteci olan tiyol/disülfit dengesinin, kullanılabilirliğini araştırmaktır. Gereç ve Yöntemler: Çalışmaya, yaşları 0-18 yaşları aralığında bulunan ve kontrol grubunda 30, akut apandisit grubunda 30 olmak üzere toplam 60 gönüllü çocuk dahil edildi. Her iki grubun da nativ tiyol, total tiyol, dinamik disülfit, dinamik tiyol, iskemi modifiye albümin, albümin, lökosit, hemoglobin, hematokrit ve trombosit sayıları bakıldı. Veriler, SPSS (Statistical Package for Social Sciences) versiyon 17 (Chicago, USA) programı kullanılarak karşılaştırıldı. Tüm değişkenler için p <0.05 anlamlı kabul edildi. Bulgular: Akut apandisit grubunda, kontrol grubu ile karşılaştırıldığında; total tiyol (p<0.001), nativ tiyol (p<0.001) ve albümin (p<0.001) düzeyleri anlamlı düzeyde düşük bulunmasına karşın, oksidatif tarafa kaymayı gösteren dinamik sülfit, dinamik tiyol (p=0.003) ve iskemi modifiye albümin (p<0.001) düzeyleri yüksek bulundu. Akut apandisit grubunda lökosit sayısı kontrol grubuna göre daha yüksek (p <0.001) bulunurken trombosit sayısı kontrol grubundan daha bulundu (p = 0.03). Sonuç: Akut apandisitin ayırıcı tanısında, özellikle teşhis konmakta zorlanılan olgularda, fizik muayene, görüntüleme ve güncel laboratuvar testlerinin yanı sıra, tiyol/disülfid dengesinin değerlendirilmesi tanı koymada yardımcı olabilir. Ek olarak albümin ve iskemi modifiye albümin düzeylerinin değerlendirilmesi testin özgüllüğünü artırabilir. Bu test, yaşları küçük olan çocuklar ve mental retarde hastalar gibi tanının zor konabildiği hastaların ayırıcı tanısında daha yararlı olabilir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Mohammed AA, Daghman NA, Aboud SM, Oshibi HO. The diagnostic value of C-reactive protein, white blood cell count and neutrophil percentage in childhood appendicitis. Saudi Med J 2004;25:1212-5.
2. Sengupta A, Bax G, Paterson-Brown S. White cell count and C-reactive protein measurement in patients with possible appendicitis. Ann R Coll Surg Engl 2009;91:113-5.
3. Alvarado A. A practical score for the early diagnosis of acute appendicitis. Ann Emerg Med 1986;15:557-64.
4. de Oliveira Machado SL, Bagatini MD, da Costa P, Baldissarelli J, Reichert KP, de Oliveira LS, et al. Evaluation of mediators of oxidative stress and inflammation in patients with acute appendicitis. Biomarkers 2016;21:530-7.
5. Groselj-Grenc M, Repse S, Dolenc-Strazar Z, Hojker S, Derganc M. Interleukin-6 and lipopolysaccharide-binding protein in acute appendicitis in children. Scand J Clin Lab Invest 2007;67:197-206.
6. Paajanen H, Mansikka A, Laato M, Ristamaki R, Pulkki K, Kostiainen S. Novel serum inflammatory markers in acute appendicitis. Scand J Clin Lab Invest 2002;62:579-84.
7. Yildirim O, Solak C, Kocer B, Unal B, Karabeyoglu M, Bozkurt B, et al. The role of serum inflammatory markers in acute appendicitis and their success in preventing negative laparotomy. J Invest Surg 2006;19:345-52.
8. Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 2007;39:44-84.
9. Ozdogan M, Devay AO, Gurer A, Ersoy E, Devay SD, Kulacoglu H, et al. Plasma total anti-oxidant capacity correlates inversely with the extent of acute appendicitis: a case control study. World J Emerg Surg 2006;1:6.
10. Serefhanoglu K, Taskin A, Turan H, Timurkaynak FE, Arslan H, Erel O. Evaluation of oxidative status in patients with brucellosis. Braz J Infect Dis 2009;13:249-51.
11. Erel O, Neselioglu S. A novel and automated assay for thiol/ disulphide homeostasis. Clin Biochem 2014;47:326-32.
12. Hu ML. Measurement of protein thiol groups and glutathione in plasma. Methods Enzymol 1994;233:380-5.
13. Circu ML, Aw TY. Reactive oxygen species, cellular redox systems, and apoptosis. Free Radic Biol Med 2010;48:749-62.
14. Abuja PM, Albertini R. Methods for monitoring oxidative stress, lipid peroxidation and oxidation resistance of lipoproteins. Clin Chim Acta 2001;306:1-17.
15. Koltuksuz U, Uz E, Ozen S, Aydinc M, Karaman A, Akyol O. Plasma superoxide dismutase activity and malondialdehyde level correlate with the extent of acute appendicitis. Pediatr Surg Int 2000;16:559-61.
16. Kaya M, Boleken ME, Kanmaz T, Erel O, Yucesan S. Total antioxidant capacity in children with acute appendicitis. Eur J Pediatr Surg 2006;16:34-8. 17. Yilmaz FM, Yilmaz G, Erol MF, Koklu S, Yucel D. Nitric oxide, lipid peroxidation and total thiol levels in acute appendicitis. J Clin Lab Anal 2010;24:63-6. 18. Karadag-Oncel E, Erel O, Ozsurekci Y, Caglayik DY, Kaya A, Gozel MG, et al. Plasma oxidative stress and total thiol levels in CrimeanCongo hemorrhagic fever. Jpn J Infect Dis 2014;67:22-6. 19. Kundi H, Ates I, Kiziltunc E, Cetin M, Cicekcioglu H, Neselioglu S, et al. A novel oxidative stress marker in acute myocardial infarction; thiol/disulphide homeostasis. Am J Emerg Med 2015;331567-71. 20. Dinc ME, Ulusoy S, Is A, Ayan NN, Avincsal MO, Bicer C, et al. Thiol/ disulphide homeostasis as a novel indicator of oxidative stress in sudden sensorineural hearing loss. J Laryngol Otol 2016;130:447- 52. 21. Gumusyayla S, Vural G, Bektas H, Neselioglu S, Deniz O, Erel O. A novel oxidative stress marker in migraine patients: dynamic thioldisulphide homeostasis. Neurol Sci 2016;37:1311-7.
22. Ozyazici S, Karateke F, Turan U, Kuvvetli A, Kilavuz H, Karakaya B, et al. A Novel Oxidative Stress Mediator in Acute Appendicitis: Thiol/ Disulphide Homeostasis. Mediators Inflamm 2016;2016:6761050.
23. Elmas B, Karacan M, Dervisoglu P, Kosecik M, Isguven SP, Bal C. Dynamic thiol/disulphide homeostasis as a novel indicator of oxidative stress in obese children and its relationship with inflammatory-cardiovascular markers. Anatol J Cardiol 2017;18:361-9.
24. Zhang HH, Gu GL, Zhang XY, Fan Q, Wang XY, Wei XM. Nonsurgical contraindication for acute appendicitis with secondary thrombocytopenia: a case report. World J Gastroenterol 2015;21:2836-9.
25. Dominguez-Rodriguez A, Abreu-Gonzalez P. Current role of ischemia-modified albumin in routine clinical practice. Biomarkers 2010;15:655-62.
26. Reddy VS, Perugu B, Garg MK. Ischemia-modified albumin must be evaluated as an oxidative stress marker together with albumin and bilirubin in individuals with acute appendicitis. Clinics (Sao Paulo) 2015;70:531-2.
27. Reddy VS, Pasupuleti P, Srinivasa Rao PV, Garg R, Haribabu A. Ischemia-modified albumin in patients with hyperthyroidism and hypothyroidism. Eur J Intern Med 2014;25:e42-3.
28. Reddy VS, Sethi S, Agrawal P, Gupta N, Garg R. Ischemia modified albumin (IMA) and albumin adjusted-IMA (AAIMA) as biomarkers for diabetic retinopathy. Nepal J Ophthalmol 2015;7:117-23.
29. Dumlu EG, Tokac M, Bozkurt B, Yildirim MB, Ergin M, Yalcin A, et al. Correlation between the serum and tissue levels of oxidative stress markers and the extent of inflammation in acute appendicitis. Clinics (Sao Paulo) 2014;69:677-82.
30. Nazik S, Avci V, Kusku Kiraz Z. Ischemia-modified albumin and other inflammatory markers in the diagnosis of appendicitis in children. Ulus Travma Acil Cerrahi Derg 2017;23:317-21.
31. Kilic MO, Guldogan CE, Balamir I, Tez M. Ischemia-modified albumin as a predictor of the severity of acute appendicitis. Am J Emerg Med 2017;35:92-5.
32. Satomi A, Hashimoto T, Murakami S, Murai H, Kawase H, Takahashi S, et al. Tissue superoxide dismutase (SOD) activity and immunohistochemical staining in acute appendicitis: correlation with degree of inflammation. J Gastroenterol 1996;31:639-45.
33. Turan C, Kucukaydin N, Dogan P, Kontas O, Bozkurt A, Kucukaydin M. The effect of acute ligation of the rabbit appendix on antioxidant enzymes. Res Exp Med (Berl) 1996;196:45-51.
34. Kavakli HS, Erel O, Becel S. Oxidative stress in diagnosis of acute appendicitis patients. Scientific Research and Essays. 2011;6:1766-70.
35. Koksal H, Kurban S, Dogru O. Total oxidant status, total antioxidant status, and paraoxonase activity in acute appendicitis. Ulus Travma Acil Cerrahi Derg 2015;21:139-42.
36. Boshnak N, Boshnaq M, Elgohary H. Evaluation of Platelet Indices and Red Cell Distribution Width as New Biomarkers for the Diagnosis of Acute Appendicitis. J Invest Surg 2017:1-9.
37. Kwan KY, Nager AL. Diagnosing pediatric appendicitis: usefulness of laboratory markers. Am J Emerg Med 2010;28:1009-15.
38. Fan Z, Pan J, Zhang Y, Wang Z, Zhu M, Yang B, et al. Mean Platelet Volume and Platelet Distribution Width as Markers in the Diagnosis of Acute Gangrenous Appendicitis. Dis Markers 2015;2015:542013.
39. Park JS, Jeong JH, Lee JI, Lee JH, Park JK, Moon HJ. Accuracies of diagnostic methods for acute appendicitis. Am Surg 2013;79:101- 6.
40. Oyetunji TA, Ong’uti SK, Bolorunduro OB, Cornwell EE, 3rd, Nwomeh BC. Pediatric negative appendectomy rate: trend, predictors, and differentials. J Surg Res 2012;173:16-20.

APA	Demir S, Dere Gunal Y, ÖZMEN İ, DINDAR BADEM N, neselioglu s, EREL O (2020). Can Thiol/Disulphide Homeostasis Help in The Differential Diagnosis of Appendicitis in Children?. , 236 - 243. 10.12956/tchd.627936
Chicago	Demir Sabri,Dere Gunal Yasemin,ÖZMEN İSMAİL,DINDAR BADEM NERMIN,neselioglu salim,EREL Ozcan Can Thiol/Disulphide Homeostasis Help in The Differential Diagnosis of Appendicitis in Children?. (2020): 236 - 243. 10.12956/tchd.627936
MLA	Demir Sabri,Dere Gunal Yasemin,ÖZMEN İSMAİL,DINDAR BADEM NERMIN,neselioglu salim,EREL Ozcan Can Thiol/Disulphide Homeostasis Help in The Differential Diagnosis of Appendicitis in Children?. , 2020, ss.236 - 243. 10.12956/tchd.627936
AMA	Demir S,Dere Gunal Y,ÖZMEN İ,DINDAR BADEM N,neselioglu s,EREL O Can Thiol/Disulphide Homeostasis Help in The Differential Diagnosis of Appendicitis in Children?. . 2020; 236 - 243. 10.12956/tchd.627936
Vancouver	Demir S,Dere Gunal Y,ÖZMEN İ,DINDAR BADEM N,neselioglu s,EREL O Can Thiol/Disulphide Homeostasis Help in The Differential Diagnosis of Appendicitis in Children?. . 2020; 236 - 243. 10.12956/tchd.627936
IEEE	Demir S,Dere Gunal Y,ÖZMEN İ,DINDAR BADEM N,neselioglu s,EREL O "Can Thiol/Disulphide Homeostasis Help in The Differential Diagnosis of Appendicitis in Children?." , ss.236 - 243, 2020. 10.12956/tchd.627936
ISNAD	Demir, Sabri vd. "Can Thiol/Disulphide Homeostasis Help in The Differential Diagnosis of Appendicitis in Children?". (2020), 236-243. https://doi.org/10.12956/tchd.627936

APA	Demir S, Dere Gunal Y, ÖZMEN İ, DINDAR BADEM N, neselioglu s, EREL O (2020). Can Thiol/Disulphide Homeostasis Help in The Differential Diagnosis of Appendicitis in Children?. Türkiye Çocuk Hastalıkları Dergisi, 14(3), 236 - 243. 10.12956/tchd.627936
Chicago	Demir Sabri,Dere Gunal Yasemin,ÖZMEN İSMAİL,DINDAR BADEM NERMIN,neselioglu salim,EREL Ozcan Can Thiol/Disulphide Homeostasis Help in The Differential Diagnosis of Appendicitis in Children?. Türkiye Çocuk Hastalıkları Dergisi 14, no.3 (2020): 236 - 243. 10.12956/tchd.627936
MLA	Demir Sabri,Dere Gunal Yasemin,ÖZMEN İSMAİL,DINDAR BADEM NERMIN,neselioglu salim,EREL Ozcan Can Thiol/Disulphide Homeostasis Help in The Differential Diagnosis of Appendicitis in Children?. Türkiye Çocuk Hastalıkları Dergisi, vol.14, no.3, 2020, ss.236 - 243. 10.12956/tchd.627936
AMA	Demir S,Dere Gunal Y,ÖZMEN İ,DINDAR BADEM N,neselioglu s,EREL O Can Thiol/Disulphide Homeostasis Help in The Differential Diagnosis of Appendicitis in Children?. Türkiye Çocuk Hastalıkları Dergisi. 2020; 14(3): 236 - 243. 10.12956/tchd.627936
Vancouver	Demir S,Dere Gunal Y,ÖZMEN İ,DINDAR BADEM N,neselioglu s,EREL O Can Thiol/Disulphide Homeostasis Help in The Differential Diagnosis of Appendicitis in Children?. Türkiye Çocuk Hastalıkları Dergisi. 2020; 14(3): 236 - 243. 10.12956/tchd.627936
IEEE	Demir S,Dere Gunal Y,ÖZMEN İ,DINDAR BADEM N,neselioglu s,EREL O "Can Thiol/Disulphide Homeostasis Help in The Differential Diagnosis of Appendicitis in Children?." Türkiye Çocuk Hastalıkları Dergisi, 14, ss.236 - 243, 2020. 10.12956/tchd.627936
ISNAD	Demir, Sabri vd. "Can Thiol/Disulphide Homeostasis Help in The Differential Diagnosis of Appendicitis in Children?". Türkiye Çocuk Hastalıkları Dergisi 14/3 (2020), 236-243. https://doi.org/10.12956/tchd.627936