Determination of Phenolic Acids by HPLC and Antioxidant Capacity of Some Cultivated Mentha Species
Yıl: 2021 Cilt: 24 Sayı: 3 Sayfa Aralığı: 488 - 494 Metin Dili: İngilizce DOI: 10.18016/ksutarimdoga.vi.780019 İndeks Tarihi: 27-07-2021
Determination of Phenolic Acids by HPLC and Antioxidant Capacity of Some Cultivated Mentha Species
Öz: This study was designed to investigate the phenolic acid contents andantioxidant activities of 14 clones of mint species (M. spicata, M.piperita, M. villoso nervata and M. dumetorum) that cultivated inTurkey. The phenolic acid contents were analysed using HPLC. Theantioxidant activities of these Mentha species were evaluated by freeradical scavenging activity (DDPH•), cation radical scavengingactivity (ABTS•+) and reducing power activity tests. Additionally, totalphenolic content of mint species was determined spectrophotometrically using Folin-Ciacelteau’s reagent. Phenolic acid contents ofMentha were evaluated in two subclasses: benzoic acid and cinnamicacid derivatives. The rosmarinic acid (which is cinnamic acidderivative) was the main phenolic acid for all tested plant materialchanging from 5482.6 to 31982.7 mg/kg dry weight concentrations. phydroxy benzoic acid (ranging from 30.5 to 236.4 mg/kg dry weight)and syringic acid (ranging from 95.4 to 375.2 mg/kg dry weight) weremost abundant benzoic acids derivatives found in tested mint clones.According to DDPH● and ABTS●+ test results, while M. villoso nervataclone (originated from Osmaniye) has higher activity, M. spicata clone(originated from Manisa) has lower activity among the other clones.Reducing power activity of M. villoso nervata clone (originated fromOsmaniye) was highest and M. spicata clone (originated from Konya)was lowest. It has been observed that the correlation between phenolicacid derivatives and antioxidant activity varied depending on usedantioxidant activity methods. These results show that best Mentha forhuman health in terms of both phenolic acid content and antioxidantactivity are the 3rd clone (M. spicata) and the 10th clone (M. villosonervata) genotypes.
Anahtar Kelime: Kültürü Yapılan Bazı Mentha Türlerinin HPLC ile Fenolik Asitlerin Tayini ve Antioksidan Kapasitesi Belirlenmesi
Öz: Bu çalışma, Türkiye'de yetiştirilen M. spicata, M. piperita, M. villoso nervata ve M. dumetorum türlerine ait 14 nane klonlarının fenolik asit içerikleri ve antioksidan aktivitelerini araştırmak için tasarlanmıştır. Fenolik asit içerikleri HPLC kullanılarak analiz edilmiştir. Mentha türlerinin antioksidan aktiviteleri, serbest radikal giderme aktivitesi (DDPH•), katyon radikal giderme aktivitesi (ABTS• +) ve indirgeme gücü aktivite testleri ile değerlendirilmiştir. Ek olarak, nane türlerinin toplam fenolik içeriği, FolinCiacelteau’nun reaktifi kullanılarak spektrofotometrik olarak belirlenmiştir. Nane türlerinin fenolik asit içerikleri benzoik ve sinamik asit türevi olmak üzere iki alt sınıfta değerlendirilmiştir. Rosmarinik asit (sinnamik asit türevi) konsantrasyonu, test edilen tüm bitki materyalleri için 5482.6'dan 31982.7 mg/kg kuru ağırlı arasında değişen ana fenolik asit olarak gözlemlenmiştir. p-hidroksi benzoik asit (30.5 ile 236.4 mg/kg kuru ağırlık arasında) ve şiringik asit (95.4 ile 375.2 mg/kg kuru ağırlık arasında), test edilen nane klonlarında en yüksek miktarda bulunan benzoik asit türevleridir. DDPH● ve ABTS●+ test sonuçlarına göre M. villoso nervata türü (Osmaniye kaynaklı klonu) daha yüksek aktiviteye sahipken, M. spicata türü (Manisa kaynaklı klonu) diğer klonlar arasında daha düşük aktiviteye sahip olduğu görülmüştür. M. villoso nervata türü (Osmaniye kaynaklı klonu) indirgeme gücü aktivitesi düşük iken en yüksek aktiviteyi M. spicata türü (Konya kaynaklı klonu) göstermiştir. Fenolik asit türevleri ile antioksidan aktivite arasındaki ilişkinin, kullanılan antioksidan aktivite yöntemlerine bağlı olarak değiştiği görülmüştür. Elde edilen sonuçlara göre hem fenolik asit içeriği hem de antioksidan aktivite açısından insan sağlığı için en yararlı nane türünün 3. klon (M. spicata) ve 10. klon (M. villoso nervata) genotipleri olduğunu tespit edilmiştir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | ELMASTAŞ M, KEÇECİ M, genç n, Beyatli A, Mısırlı D, Telci İ (2021). Determination of Phenolic Acids by HPLC and Antioxidant Capacity of Some Cultivated Mentha Species. , 488 - 494. 10.18016/ksutarimdoga.vi.780019 |
| Chicago | ELMASTAŞ MAHFUZ,KEÇECİ Mehmet,genç nusret,Beyatli Ahmet,Mısırlı Duygu,Telci İsa Determination of Phenolic Acids by HPLC and Antioxidant Capacity of Some Cultivated Mentha Species. (2021): 488 - 494. 10.18016/ksutarimdoga.vi.780019 |
| MLA | ELMASTAŞ MAHFUZ,KEÇECİ Mehmet,genç nusret,Beyatli Ahmet,Mısırlı Duygu,Telci İsa Determination of Phenolic Acids by HPLC and Antioxidant Capacity of Some Cultivated Mentha Species. , 2021, ss.488 - 494. 10.18016/ksutarimdoga.vi.780019 |
| AMA | ELMASTAŞ M,KEÇECİ M,genç n,Beyatli A,Mısırlı D,Telci İ Determination of Phenolic Acids by HPLC and Antioxidant Capacity of Some Cultivated Mentha Species. . 2021; 488 - 494. 10.18016/ksutarimdoga.vi.780019 |
| Vancouver | ELMASTAŞ M,KEÇECİ M,genç n,Beyatli A,Mısırlı D,Telci İ Determination of Phenolic Acids by HPLC and Antioxidant Capacity of Some Cultivated Mentha Species. . 2021; 488 - 494. 10.18016/ksutarimdoga.vi.780019 |
| IEEE | ELMASTAŞ M,KEÇECİ M,genç n,Beyatli A,Mısırlı D,Telci İ "Determination of Phenolic Acids by HPLC and Antioxidant Capacity of Some Cultivated Mentha Species." , ss.488 - 494, 2021. 10.18016/ksutarimdoga.vi.780019 |
| ISNAD | ELMASTAŞ, MAHFUZ vd. "Determination of Phenolic Acids by HPLC and Antioxidant Capacity of Some Cultivated Mentha Species". (2021), 488-494. https://doi.org/10.18016/ksutarimdoga.vi.780019 |
| APA | ELMASTAŞ M, KEÇECİ M, genç n, Beyatli A, Mısırlı D, Telci İ (2021). Determination of Phenolic Acids by HPLC and Antioxidant Capacity of Some Cultivated Mentha Species. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 24(3), 488 - 494. 10.18016/ksutarimdoga.vi.780019 |
| Chicago | ELMASTAŞ MAHFUZ,KEÇECİ Mehmet,genç nusret,Beyatli Ahmet,Mısırlı Duygu,Telci İsa Determination of Phenolic Acids by HPLC and Antioxidant Capacity of Some Cultivated Mentha Species. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 24, no.3 (2021): 488 - 494. 10.18016/ksutarimdoga.vi.780019 |
| MLA | ELMASTAŞ MAHFUZ,KEÇECİ Mehmet,genç nusret,Beyatli Ahmet,Mısırlı Duygu,Telci İsa Determination of Phenolic Acids by HPLC and Antioxidant Capacity of Some Cultivated Mentha Species. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, vol.24, no.3, 2021, ss.488 - 494. 10.18016/ksutarimdoga.vi.780019 |
| AMA | ELMASTAŞ M,KEÇECİ M,genç n,Beyatli A,Mısırlı D,Telci İ Determination of Phenolic Acids by HPLC and Antioxidant Capacity of Some Cultivated Mentha Species. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. 2021; 24(3): 488 - 494. 10.18016/ksutarimdoga.vi.780019 |
| Vancouver | ELMASTAŞ M,KEÇECİ M,genç n,Beyatli A,Mısırlı D,Telci İ Determination of Phenolic Acids by HPLC and Antioxidant Capacity of Some Cultivated Mentha Species. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. 2021; 24(3): 488 - 494. 10.18016/ksutarimdoga.vi.780019 |
| IEEE | ELMASTAŞ M,KEÇECİ M,genç n,Beyatli A,Mısırlı D,Telci İ "Determination of Phenolic Acids by HPLC and Antioxidant Capacity of Some Cultivated Mentha Species." Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 24, ss.488 - 494, 2021. 10.18016/ksutarimdoga.vi.780019 |
| ISNAD | ELMASTAŞ, MAHFUZ vd. "Determination of Phenolic Acids by HPLC and Antioxidant Capacity of Some Cultivated Mentha Species". Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 24/3 (2021), 488-494. https://doi.org/10.18016/ksutarimdoga.vi.780019 |
