Yıl: 2021 Cilt: 45 Sayı: 1 Sayfa Aralığı: 1 - 14 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Presowing seed treatment with glycine betaine confers NaCl tolerance in quinoa by modulating some physiological processes and antioxidant machinery

Öz:
Soil salinity threatens the yield and food security worldwide and use of halophytes might be a valid option to cope with devastating salinity effects. The present investigation was accomplished to evaluate the role of glycine betaine (GB) as presowing seed treatment on growth parameters, photosynthetic pigments, gas exchange attributes, enzymatic antioxidants, lipid peroxidation (MDA), hydrogen peroxide (H2O2), endogenous GB, total soluble proteins (TSP), and yield in quinoa (Chenopodium quinoa) under saline conditions. Ames-13737 (Q7) and PI-634919 (Q9) quinoa accessions were used for this trial. The plants were applied with two salt stress treatments (Control and 450 mM NaCl) after 45 days of sowing. Presowing seed treatments with GB (water, 10 and 20 mM) were given for 12 h. Each treatment replicated four times through completely randomized design. Imposition of salinity triggered a major decrease in growth, photosynthetic pigments, net CO2 assimilation rate (A), transpiration rate (E), and stomatal conductance (gs), while the level of lipid peroxidation and activities of superoxide dismutase (SOD) and catalase (CAT) were enhanced. Slight increment in total soluble proteins was observed along with higher endogenous GB in quinoa under salinity. Both levels of GB increased the shoot length, shoot fresh, and dry weights in accession PI-634919 under saline regime. Photosynthetic attributes, E and gs were increased when 20 mM of GB was applied under saline conditions. Activities of antioxidant enzymes, TSP, endogenous glycine betaine and yield parameters were also increased when GB was applied. Presowing treatment with GB in quinoa plants prominently decreased the MDA and H2O2 concentration. The concentration of 10 mM GB enhanced the panicle length while 20 mM GB showed same results for 1000 seed weight and TSP under saline conditions. Overall, quinoa accession Ames-13737 was better as compared to PI-634919 in terms of growth rate, photosynthetic properties, gas exchange parameters, enzymatic antioxidants, and yield.
Anahtar Kelime: yield Quinoa glycine betaine salinity enzymatic antioxidants

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  • Abbas W, Ashraf M, Akram NA (2010). Alleviation of saltinduced adverse effects in eggplant (Solanum melongena L.) by glycinebetaine and sugarbeet extracts. Scientia Horticulturae 125: 188-195.
  • Abdallah MM, El-Sebai TN, Ramadan AAE, El-Bassiouny HMS (2020). Physiological and biochemical role of proline, trehalose, and compost on enhancing salinity tolerance of quinoa plant. Bulletin of the Natural Research Centre 44: 1-13.
  • Abdel-Mawgoud AMR (2017). Soil and foliar applications of glycine betaine ameliorate salinity effects on squash plants grown under Bahraini conditions. Middle East Journal 6: 315-322.
  • Adolf VI, Shabala S, Andersen MN, Razzaghi F, Jacobsen SE (2012). Varietal difference of quinoa’s tolerance to saline conditions. Plant Soil 357: 117-129.
  • Ahanger MA, Qin C, Maodong Q, Dong XX, Ahmad P et al. (2019). Spermine application alleviates salinity induced growth and photosynthetic inhibition in Solanum lycopersicum by modulating osmolyte and secondary metabolite accumulation and differentially regulating antioxidant metabolism. Plant Physiology and Biochemistry 144: 1-13.
  • Alasvandyari F, Mahdavi B (2018). Effect of glycine betaine and salinity on photosynthetic pigments and ion concentration of safflower. Desert 23: 265-271.
  • Alasvandyari, F., B. Mahdavi and S. M. Hosseini. 2017. Glycine betaine affects the antioxidant system and ion accumulation and reduces salinity-induced damage in safflower seedlings. Archives of Biological Sciences 69: 139-147.
  • Al-Dakheel AJ, Hussain MI (2016). Genotypic variation for salinity tolerance in Cenchrus ciliaris L. Frontier in Plant Science 7: 1-12.
  • Arfan M, Habib RA, Ashraf M (2007). Does exogenous application of salicylic acid through the rooting medium modulate growth and photosynthetic capacity in two differentially adapted spring wheat cultivars under salt stress? Journal of Plant Physiology 164: 685-694.
  • Arnon DT (1949). Copper enzyme in isolated chloroplasts polyphenoloxidase in Beta vulgaris. Plant Physiology 24: 1-15.
  • Ashraf M, Foolad MR (2007). Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany 59: 206-216.
  • Ashraf M, Harris PJC (2013). Photosynthesis under stressful environments: an overview. Photosynthetica 51: 163-190.
  • Ashraf MA, Akbar A, Parveen A, Rasheed R, Hussain I et al. (2018). Phenological application of selenium differentially improves growth, oxidative defense and ion homeostasis in maize under salinity stress. Plant Physiology and Biochemistry 123: 268- 280.
  • Athar H, Zafar ZU, Ashraf M (2015). Glycinebetaine improved photosynthesis in canola under salt stress: evaluation of chlorophyll fluorescence parameters as potential indicators. Journal of Agronomy and Crop Science 201: 428-442.
  • Bradford MM (1976). A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72: 248-254.
  • Carmak I, Horst JH. 1991. Effects of aluminum on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean (Glycine max). Physiologia Plantarum 83: 463- 468.
  • Campo S, Baldrich P, Messeguer J, Lalanne E, Coca M et al. (2014). Overexpression of a calcium-dependent protein kinase confers salt and drought tolerance in rice by preventing membrane lipid peroxidation. Plant Physiology 165: 688-704.
  • Chance B, Maehly AC (1955). Assay of catalase and peroxidases. Methods in Enzymology 2: 764-775.
  • Chen TH, Murata N (2008). Glycinebetaine: an effective protectant against abiotic stress in plants. Trends in Plant Science 13: 499- 505.
  • Derbali W, Goussi R, Koyro HW, Abdelly C, Manaa A (2020). Physiological and biochemical markers for screening salt tolerant quinoa genotypes at early seedling stage. Journal of Plant Interactions 15: 27-38.
  • El-Esawi MA, Alaraidh IA, Alsahli AA, Alamri SA, Ali HM et al. (2018). Bacillus firmus (SW5) augments salt tolerance in soybean (Glycine max L.) by modulating root system architecture, antioxidant defense systems and stress-responsive genes expression. Plant Physiology and Biochemistry 132: 375- 384.
  • Estaji A, Kalaji HM, Karimi HR, Roosta HR, Moosavi-Nezhad SM (2019). How glycine betaine induces tolerance of cucumber plants to salinity stress? Photosynthetica 57: 753-761.
  • Farooq M, Wahid A, Kobayashi N, Fujita D, Basra SMA (2009). Plant drought stress: effects, mechanisms and management. In: Lichtfouse E, Navarrete M, Debaeke P, Véronique S, Alberola C (editors). Sustainable Agriculture. Dordrechti Netherlands: Springer, pp. 153-188.
  • Genard H, Le-Saos J, Billard JP, Tremolieres A, Boucaud J (1991). Effect of salinity on lipid composition, glycine betaine content and photosynthetic activity in chloroplasts of Suaeda maritima. Plant Physiology and Biochemistry 29: 421-427.
  • Giannopolitis CN, Ries SK (1977). Superoxide dismutase: I. Occurrence in higher plants. Plant Physiology 59: 309-314.
  • Gupta B, Huang B (2014). Mechanism of salinity tolerance in plants: physiological, biochemical, and molecular characterization. International Journal of Genomics 2014: 1-18.
  • Hasanuzzaman M, Alam M, Rahman A, Hasanuzzaman M, Nahar K et al. (2014). Exogenous proline and glycine betaine mediated upregulation of antioxidant defense and glyoxalase systems provides better protection against salt-induced oxidative stress in two rice (Oryza sativa L.) varieties. BioMed Research International 2014: 1-17.
  • Hasanuzzaman M, Nahar K, Fujita M (2013). Plant response to salt stress and role of exogenous protectants to mitigate saltinduced damages. In: Ahmad P, Azooz MM, Prasad MNV (editors). Ecophysiology and Responses of Plants Under Salt Stress. New York, NY, USA:Springer, pp. 25-87.
  • Hoque MA, Banu MNA, Okuma E, Amako K, Nakamura Y et al. (2007). Exogenous proline and glycinebetaine increase NaClinduced ascorbate glutathione cycle enzyme activities, and proline improves salt tolerance more than glycinebetaine in tobacco Bright Yellow-2 suspension-cultured cells. Journal of Plant Physiology 164: 1457-1468.
  • Hu LHT, Zhang X, Pang H, Fu J (2012). Exogenous glycine betaine ameliorates the adverse effect of salt stress on perennial ryegrass. Journal of the American Society for Horticultural Science 137: 38-46.
  • Hussain S, Khalid MF, Saqib M, Ahmad S, Zafar W et al. (2018). Drought tolerance in citrus rootstocks is associated with better antioxidant defense mechanism. Acta Physiologiae Plantarum 40: 1-10.
  • Iqbal S, Basra SMA, Afzal I, Wahid A (2017). Exploring potential of well adapted quinoa lines for salt tolerance. International Journal of Agriculture and Biology 19: 933-940.
  • Kausar F, Shahbaz M (2017). Influence of strigolactone (GR24) as a seed treatment on growth, gas exchange and chlorophyll fluorescence of wheat under saline conditions. International Journal of Agriculture and Biology 19: 321-327.
  • Kausar N, Nawaz K, Hussain K, Bhatti KH, Siddiqi EH et al. (2014). Effect of exogenous application of glycine betaine on growth and gaseous exchange attributes of two maize (Zea mays L.) cultivars under saline conditions. World Applied Sciences Journal 29: 1559-1565.
  • Kotb MA, Elhamahmy MA (2014). Improvement of wheat productivity and their salt tolerance by exogenous glycine betaine application under saline soil condition for longterm. Zagazig Journal of Agricultural Research 41: 1127-1143.
  • Lalarukh I, Shahbaz M (2018) Alpha-tocopherol induced modulations in morpho-physiological attributes of sunflower (Helianthus annuus) when grown under saline environment. International Journal of Agriculture and Biology 20: 661-668.
  • Malekzadeh P (2015). Influence of exogenous application of glycinebetaine on antioxidative system and growth of salt stressed soybean seedlings (Glycine max L.). Physiology and Molecular Biology of Plants 21: 225-232.
  • Meneguetti QA, Brenzan MA, Batista MR, Bazotte RB, Silva DR (2011). Biological effects of hydrolyzed quinoa extract from seeds of Chenopodium quinoa Willd. Journal of Medicinal Food 14: 653-657.
  • Morrison CD, Laeger T (2015). Protein-dependent regulation of feeding and metabolism. Trends in Endocrinology and Metabolism 26: 256-262.
  • Nawaz M, Wang Z (2020). Abscisic acid and glycine betaine mediated tolerance mechanisms under drought stress and recovery in Axonopus compressus: a new insight. Scientific Reports 10: 1-10.
  • Negi J, Hashimoto-Sugimoto M, Kusumi K, Iba K (2014). New approaches to the biology of stomatal guard cells. Plant and Cell Phyisology 55: 241-250.
  • Raza SH, Athar HR, Ashraf M, Hameed A (2007). Glycinebetaineinduced modulation of antioxidant enzymes activities and ion accumulation in two wheat cultivars differing in salt tolerance. Environmental and Experimental Botany 60: 368-376.
  • Razzaghi F, Ahmadi SH, Jacobsen SE, Jensen CR, Andersen MN (2012). Effects of salinity and soil–drying on radiation use efficiency, water productivity and yield of quinoa (Chenopodium quinoa Willd.). Journal of Agronomy and Crop Science 198: 173-184.
  • Ruiz KB, Biondi S, Martínez EA, Orsini F, Antognoni F et al. (2016). Quinoa-A model crop for understanding salt-tolerance mechanisms in halophytes. Plant Biosystems 150: 357-371.
  • Saeed HM, Mirza JI, Anjum MA (2016). Glycinebetaine-induced modulations in some biochemical and physiological attributes of okra under salt stress. Pakistan Journal of Botany 48: 2205- 2210.
  • Shafiq S, Akram NA, Ashraf M (2015). Does exogenously-applied trehalose alter oxidative defense system in the edible part of radish (Raphanus sativus L.) under water-deficit conditions? Scientia Horticulturae 85: 68-75.
  • Shahbaz M, Abid A, Masood A, Waraich EA (2017). Foliar-applied trehalose modulates growth, mineral nutrition, photosynthetic ability and oxidative defense system of rice (Oryza sativa L.) under saline stress. Journal of Plant Nutrition 40: 585-599.
  • Shahbaz M, Zia B (2011). Does exogenous application of glycinebetaine through rooting medium alter rice (Oryza sativa L.) mineral nutrient status under saline conditions? Journal of Applied Botany and Food Quality 84: 54-60.
  • Silva CL, Benin G, Bornhofen E, Beche E, Todeschini MH et al. (2014). Nitrogen use efficiency is associated with chlorophyll content in Brazilian spring wheat. Australian Journal of Crop Science 8: 957-964.
  • Sofy MR, Elhawat N, Alshaal T (2020). Glycine betaine counters salinity stress by maintaining high K+/Na+ ratio and antioxidant defense via limiting Na+ uptake in common bean (Phaseolus vulgaris L.). Ecotoxicology and Environmental Safety 200: 110732.
  • Steel RCD, Torrie JH, Deeky DA (1996). Principles and procedures of statistics a biometric approach. New York, NY, USA: McGrawHill Book Company.
  • Sudhir P, Murthy SDS (2004). Effects of salt stress on basic processes of photosynthesis. Photosynthetica 42: 481-486.
  • Tariq A, Shahbaz M (2020). Glycinebetaine induced modulation in oxidative defense system and mineral nutrients sesame (Sesamum indicum L.) under saline regimes. Pakistan Journal of Botany 52: 775-782.
  • Tezara W, Mitchell V, Driscoll SP, Lawlor DW (2002). Effects of water deficit and its interaction with CO2 supply on the biochemistry and physiology of photosynthesis in sunflower. Journal of Experimental Botany 53: 1781-1791.
  • Velikova V, Yordano I, Edreva A (2000). Oxidative stress and some antioxidant systems in acid rain-treated bean plants: protective role of exogenous polyamines. Plant Science 151: 59-66.
  • Wahid A, Close TJ (2007). Expression of dehydrins under heat stress and their relationship with water relations of sugarcane leaves. Biologia Plantarum 51: 104-109.
  • Waqas M, Yaninga C, Iqbal H, Shareef M, Hafeez-ur-Rehman et al. (2019). Soil drenching of paclobutrazol: An efficient way to improve quinoa performance under salinity. Physiologia Plantarum 165: 219-231.
  • Yang X, Lu C (2005) Photosynthesis is improved by exogenous glycinebetaine in salt‐stressed maize plants. Physiologia Plantarum 124: 343-352.
  • Yildirim E, Ekinci M, Turan M, Dursun A, Kul R et al. (2015). Role of glycine betaine in mitigating deleterious effect of salt stress on lettuce (Lactuca sativa L.). Archives of Agronomy and Soil Science 61: 1673-1689.
APA MAQSOOD M, Shahbaz M, Arfan M, BASRA S (2021). Presowing seed treatment with glycine betaine confers NaCl tolerance in quinoa by modulating some physiological processes and antioxidant machinery. , 1 - 14.
Chicago MAQSOOD Muhammad Faisal,Shahbaz Muhammad,Arfan Muhammad,BASRA Shahzad Maqsood Ahmed Presowing seed treatment with glycine betaine confers NaCl tolerance in quinoa by modulating some physiological processes and antioxidant machinery. (2021): 1 - 14.
MLA MAQSOOD Muhammad Faisal,Shahbaz Muhammad,Arfan Muhammad,BASRA Shahzad Maqsood Ahmed Presowing seed treatment with glycine betaine confers NaCl tolerance in quinoa by modulating some physiological processes and antioxidant machinery. , 2021, ss.1 - 14.
AMA MAQSOOD M,Shahbaz M,Arfan M,BASRA S Presowing seed treatment with glycine betaine confers NaCl tolerance in quinoa by modulating some physiological processes and antioxidant machinery. . 2021; 1 - 14.
Vancouver MAQSOOD M,Shahbaz M,Arfan M,BASRA S Presowing seed treatment with glycine betaine confers NaCl tolerance in quinoa by modulating some physiological processes and antioxidant machinery. . 2021; 1 - 14.
IEEE MAQSOOD M,Shahbaz M,Arfan M,BASRA S "Presowing seed treatment with glycine betaine confers NaCl tolerance in quinoa by modulating some physiological processes and antioxidant machinery." , ss.1 - 14, 2021.
ISNAD MAQSOOD, Muhammad Faisal vd. "Presowing seed treatment with glycine betaine confers NaCl tolerance in quinoa by modulating some physiological processes and antioxidant machinery". (2021), 1-14.
APA MAQSOOD M, Shahbaz M, Arfan M, BASRA S (2021). Presowing seed treatment with glycine betaine confers NaCl tolerance in quinoa by modulating some physiological processes and antioxidant machinery. Turkish Journal of Botany, 45(1), 1 - 14.
Chicago MAQSOOD Muhammad Faisal,Shahbaz Muhammad,Arfan Muhammad,BASRA Shahzad Maqsood Ahmed Presowing seed treatment with glycine betaine confers NaCl tolerance in quinoa by modulating some physiological processes and antioxidant machinery. Turkish Journal of Botany 45, no.1 (2021): 1 - 14.
MLA MAQSOOD Muhammad Faisal,Shahbaz Muhammad,Arfan Muhammad,BASRA Shahzad Maqsood Ahmed Presowing seed treatment with glycine betaine confers NaCl tolerance in quinoa by modulating some physiological processes and antioxidant machinery. Turkish Journal of Botany, vol.45, no.1, 2021, ss.1 - 14.
AMA MAQSOOD M,Shahbaz M,Arfan M,BASRA S Presowing seed treatment with glycine betaine confers NaCl tolerance in quinoa by modulating some physiological processes and antioxidant machinery. Turkish Journal of Botany. 2021; 45(1): 1 - 14.
Vancouver MAQSOOD M,Shahbaz M,Arfan M,BASRA S Presowing seed treatment with glycine betaine confers NaCl tolerance in quinoa by modulating some physiological processes and antioxidant machinery. Turkish Journal of Botany. 2021; 45(1): 1 - 14.
IEEE MAQSOOD M,Shahbaz M,Arfan M,BASRA S "Presowing seed treatment with glycine betaine confers NaCl tolerance in quinoa by modulating some physiological processes and antioxidant machinery." Turkish Journal of Botany, 45, ss.1 - 14, 2021.
ISNAD MAQSOOD, Muhammad Faisal vd. "Presowing seed treatment with glycine betaine confers NaCl tolerance in quinoa by modulating some physiological processes and antioxidant machinery". Turkish Journal of Botany 45/1 (2021), 1-14.