Eff ects of Diff erent Degrees of Cold Stress on FIAF Expression in Pigs
Yıl: 2021 Cilt: 27 Sayı: 2 Sayfa Aralığı: 135 - 140 Metin Dili: İngilizce DOI: 10.9775/kvfd.2020.24832 İndeks Tarihi: 01-10-2021
Eff ects of Diff erent Degrees of Cold Stress on FIAF Expression in Pigs
Öz: AbstractCold stress is the main stressor restricting the development of animal husbandry in cold regions. Fasting-induced adipose factor (FIAF), also known as angiopoietin-like protein 4 (ANGPTL4), plays an important regulatory role in the metabolism of lipids. Its functions include inhibiting lipoprotein lipase (LPL) to eliminate triglycerides and free fatty acids in blood, reducing fat deposition and promoting adipose tissue degradation. This experiment was designed to investigate the eff ects of diff erent degrees of cold stimulation on fat metabolism in finishing pigs. Growing and fattening pigs were randomly divided into diff erent groups and exposed to temperatures of -10±2°C, -5±2°C, 0±2°C, 5±2°C and 21±2°C for 2 h. Serum, liver, neck, abdominal subcutaneous and mesenteric adipose tissues were collected and analyzed by Real-Time quantitative PCR (qRT-PCR), western blotting and enzyme-linked immunosorbent assay (ELISA) to examine FIAF expression. The results showed that a gradual increase in cold stress intensity resulted in a gradual increase in FIAF mRNA and protein expression levels in liver, neck, abdomen and mesenteric adipose tissues and FIAF concentration also gradually increased in the blood. It indicated that FIAF is involved in energy and fat metabolism in response to cold stress and may be regulated by the activation of peroxisome proliferator-activated receptor (PPAR) by free fatty acids in the blood induced by cold stress.
Anahtar Kelime: Farklı Derecelerde Soğuk Stresinin Domuzlarda FIAF Ekspresyonuna Etkileri
Öz: Soğuk stresi, soğuk bölgelerde hayvancılığın gelişmesini kısıtlayan ana stres faktörüdür. Anjiopoietin benzeri protein 4 (ANGPTL4) olarak da bilinenFasting-induced adipose faktör (FIAF), yağların metabolizmasında önemli bir düzenleyici rol oynar. FIAF’ın fonksiyonları arasında, kandaki trigliseritlerive serbest yağ asitlerini uzaklaştırmak için lipoprotein lipazı (LPL) inhibe etmek, yağ birikimini azaltmak ve yağ dokunun bozulmasını teşvik etmekbulunur. Bu çalışma, farklı derecelerde soğuk uyarımın, besili domuzlarda karaciğerin FIAF ve yağ metabolizması üzerine etkilerini araştırmak içintasarlanmıştır. Ergin ve besili domuzlar rastgele farklı gruplara ayrıldı ve 2 saat boyunca -10±2°C, -5±2°C, 0±2°C, 5±2°C ve 21±2°C sıcaklıklara maruzbırakıldı. Serum, karaciğer, boyun yağ dokusu, abdominal subkutanöz yağ doku ve mezenterik yağ doku toplandı ve FIAF ekspresyon analizi, kantitatifreal-time PCR (qRT-PCR), Western Blot ve Enzim-işaretli immünosorbent test (ELISA) ile gerçekleştirildi. Sonuçlar, soğuk stres yoğunluğundaki kademeliartışın, karaciğer, boyun yağ dokusu, karın yağ dokusu ve mezenterik yağ dokusunda FIAF’ın mRNA ve protein ekspresyon seviyelerinde kademeli birartışa neden olduğunu ve ayrıca kanda FIAF konsantrasyonunun da kademeli olarak arttığını gösterdi. FIAF’ın soğuk stresine yanıt olarak enerji ve yağmetabolizmasında rol oynadığını ve soğuk stresi ile indüklenen kandaki serbest yağ asitleri tarafından Peroksizom proliferatör-aktive reseptör (PPAR)aktivasyonu ile düzenlenebilir olduğunu gösterdi.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA | Ji H, SHAO s, LIU l, Zhen L, Niu C, Guo J, XU x, ZHAN z, LIU l, WANG w (2021). Eff ects of Diff erent Degrees of Cold Stress on FIAF Expression in Pigs. , 135 - 140. 10.9775/kvfd.2020.24832 |
Chicago | Ji Hong,SHAO shao,LIU liu,Zhen Li,Niu Chunyang,Guo Jingru,XU xu,ZHAN zhan,LIU liu,WANG wang Eff ects of Diff erent Degrees of Cold Stress on FIAF Expression in Pigs. (2021): 135 - 140. 10.9775/kvfd.2020.24832 |
MLA | Ji Hong,SHAO shao,LIU liu,Zhen Li,Niu Chunyang,Guo Jingru,XU xu,ZHAN zhan,LIU liu,WANG wang Eff ects of Diff erent Degrees of Cold Stress on FIAF Expression in Pigs. , 2021, ss.135 - 140. 10.9775/kvfd.2020.24832 |
AMA | Ji H,SHAO s,LIU l,Zhen L,Niu C,Guo J,XU x,ZHAN z,LIU l,WANG w Eff ects of Diff erent Degrees of Cold Stress on FIAF Expression in Pigs. . 2021; 135 - 140. 10.9775/kvfd.2020.24832 |
Vancouver | Ji H,SHAO s,LIU l,Zhen L,Niu C,Guo J,XU x,ZHAN z,LIU l,WANG w Eff ects of Diff erent Degrees of Cold Stress on FIAF Expression in Pigs. . 2021; 135 - 140. 10.9775/kvfd.2020.24832 |
IEEE | Ji H,SHAO s,LIU l,Zhen L,Niu C,Guo J,XU x,ZHAN z,LIU l,WANG w "Eff ects of Diff erent Degrees of Cold Stress on FIAF Expression in Pigs." , ss.135 - 140, 2021. 10.9775/kvfd.2020.24832 |
ISNAD | Ji, Hong vd. "Eff ects of Diff erent Degrees of Cold Stress on FIAF Expression in Pigs". (2021), 135-140. https://doi.org/10.9775/kvfd.2020.24832 |
APA | Ji H, SHAO s, LIU l, Zhen L, Niu C, Guo J, XU x, ZHAN z, LIU l, WANG w (2021). Eff ects of Diff erent Degrees of Cold Stress on FIAF Expression in Pigs. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 27(2), 135 - 140. 10.9775/kvfd.2020.24832 |
Chicago | Ji Hong,SHAO shao,LIU liu,Zhen Li,Niu Chunyang,Guo Jingru,XU xu,ZHAN zhan,LIU liu,WANG wang Eff ects of Diff erent Degrees of Cold Stress on FIAF Expression in Pigs. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 27, no.2 (2021): 135 - 140. 10.9775/kvfd.2020.24832 |
MLA | Ji Hong,SHAO shao,LIU liu,Zhen Li,Niu Chunyang,Guo Jingru,XU xu,ZHAN zhan,LIU liu,WANG wang Eff ects of Diff erent Degrees of Cold Stress on FIAF Expression in Pigs. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.27, no.2, 2021, ss.135 - 140. 10.9775/kvfd.2020.24832 |
AMA | Ji H,SHAO s,LIU l,Zhen L,Niu C,Guo J,XU x,ZHAN z,LIU l,WANG w Eff ects of Diff erent Degrees of Cold Stress on FIAF Expression in Pigs. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2021; 27(2): 135 - 140. 10.9775/kvfd.2020.24832 |
Vancouver | Ji H,SHAO s,LIU l,Zhen L,Niu C,Guo J,XU x,ZHAN z,LIU l,WANG w Eff ects of Diff erent Degrees of Cold Stress on FIAF Expression in Pigs. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2021; 27(2): 135 - 140. 10.9775/kvfd.2020.24832 |
IEEE | Ji H,SHAO s,LIU l,Zhen L,Niu C,Guo J,XU x,ZHAN z,LIU l,WANG w "Eff ects of Diff erent Degrees of Cold Stress on FIAF Expression in Pigs." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 27, ss.135 - 140, 2021. 10.9775/kvfd.2020.24832 |
ISNAD | Ji, Hong vd. "Eff ects of Diff erent Degrees of Cold Stress on FIAF Expression in Pigs". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 27/2 (2021), 135-140. https://doi.org/10.9775/kvfd.2020.24832 |