OVGP1 Expression in BOEC and Oviduct: An Immunohistochemicaland Immunocytochemical Study

Türk, Mustafa; Kocamis, Hakan; Karahan, Siyami; KURUM, AYTUL; OZKABADAYI, YASIN

doi:10.9775/kvfd.2020.24902

OVGP1 Expression in BOEC and Oviduct: An Immunohistochemicaland Immunocytochemical Study

Aytül KÜRÜM, (Kırıkkale Üniversitesi, Veteriner Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Kırıkkale, Türkiye)

Hakan KOCAMIŞ, (Kırıkkale Üniversitesi, Veteriner Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Kırıkkale, Türkiye)

Siyami KARAHAN, (Kırıkkale Üniversitesi, Veteriner Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Kırıkkale, Türkiye)

Mustafa TÜRK, (Kırıkkale Üniversitesi, Mühendislik Fakültesi, Biyomühendislik Bölümü, Kırıkkale, Türkiye)

Yasin ÖZKABADAYI (Kırıkkale Üniversitesi, Veteriner Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Kırıkkale, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

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Yıl: 2021 Cilt: 27 Sayı: 2 Sayfa Aralığı: 151 - 158 Metin Dili: İngilizce DOI: 10.9775/kvfd.2020.24902 İndeks Tarihi: 01-10-2021

OVGP1 Expression in BOEC and Oviduct: An Immunohistochemicaland Immunocytochemical Study

Öz:

Oviduct is an important tubular organ fostering critical physiological processes such as transport of gametes and embryos, capacitation, fertilization, early embryo development, and maturation of gametes. The aim of the present study was to evaluate oviduct specific glycoprotein-1 (OVGP1) expression in the oviduct regions at diff erent phases of the sexual cycle and bovine oviduct epithelial cells (BOEC). In the study, oviduct samples collected from 6 cows in estral and luteal phases were used. The oviduct samples were collected from the ampulla, isthmus and fimbria and evaluated through routine histology and immunohistochemical studies for OVGP1. The primary BOEC were obtained from the ampulla region and characterized by cytokeratin expression. The immunohistochemistry assay indicated that OVGP1 is expressed in secretory cells of the bovine oviduct. OVGP1 expression varies by the oviduct regions and phases of the sexual cycle. Changes in OVGP1 expression during the sexual cycle suggestively indicates a hormonal infl uence. Regional diff erence in OVGP1 expression is most likely related to the physiological events that occur in diff erent regions of the oviduct. BOEC isolated from the oviduct of estral and luteal phases also expresses OVGP1. Further studies should focus on possible role of OVGP1 in adaption of BOEC to very tedious condition like cell culture.

Anahtar Kelime:

BOEC ve Ovidukt’ta OVGP1 Ekspresyonu: İmmunohistokimyasal ve İmmunositokimyasal Çalışm

Öz:

Ovidukt, gametlerin olgunlaşması, erken dönemde embriyo gelişimi fertilizasyon, kapasitasyon embriyo ve gametlerin taşınması gibikritik fizyolojik süreçleri destekleyen önemli bir tubuler organdır. Bu çalışmanın amacı, seksüel siklusun farklı evrelerinde oviduktunbölgelerinde ve sığır ovidukt epitel hücrelerinde (BOEC) ovidukta özgü glikoprotein-1 (OVGP1) ekspresyonunu değerlendirmektir. Buçalışmada östral ve luteal dönemdeki 6’şar inekten alınan ovidukt örnekleri kullanıldı. Oviduktun ampulla, istmus ve fimbriya bölgelerindenalınan örneklerde, OVGP1 immünohistokimyasal çalışmalarla değerlendirildi. BOEC ampulla bölgesinden elde edildi ve sitokeratinekspresyonu ile karakterize edildi. İmmünohistokimya ile, OVGP1’in sığır oviduktunda özellikle sekretorik hücrelerde bulunduğu gösterildi.OVGP1 ekspresyonu, oviduktun bölgelerine ve seksüel siklusun dönemlerine göre değişmektedir. Seksüel siklus sırasında OVGP1ekspresyonundaki değişiklikler muhtemel bir hormonal bir etkiyi düşündürür. OVGP1 ekspresyonundaki bölgesel farklılık, büyük olasılıklayumurta kanalının farklı bölgelerinde meydana gelen fizyolojik olaylarla ilgilidir. Estral ve luteal dönemlerde ovidukttan izole edilenBOEC’te OVGP1’i ekspresse etmektedir. Bundan sonraki çalışmalar, OVGP1’in BOEC’in zorlu hücre kültürü şartlarına, adaptasyonundakirolüne odaklanmalıdır.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KURUM A, Kocamis H, Karahan S, Türk M, OZKABADAYI Y (2021). OVGP1 Expression in BOEC and Oviduct: An Immunohistochemicaland Immunocytochemical Study. , 151 - 158. 10.9775/kvfd.2020.24902
Chicago	KURUM AYTUL,Kocamis Hakan,Karahan Siyami,Türk Mustafa,OZKABADAYI YASIN OVGP1 Expression in BOEC and Oviduct: An Immunohistochemicaland Immunocytochemical Study. (2021): 151 - 158. 10.9775/kvfd.2020.24902
MLA	KURUM AYTUL,Kocamis Hakan,Karahan Siyami,Türk Mustafa,OZKABADAYI YASIN OVGP1 Expression in BOEC and Oviduct: An Immunohistochemicaland Immunocytochemical Study. , 2021, ss.151 - 158. 10.9775/kvfd.2020.24902
AMA	KURUM A,Kocamis H,Karahan S,Türk M,OZKABADAYI Y OVGP1 Expression in BOEC and Oviduct: An Immunohistochemicaland Immunocytochemical Study. . 2021; 151 - 158. 10.9775/kvfd.2020.24902
Vancouver	KURUM A,Kocamis H,Karahan S,Türk M,OZKABADAYI Y OVGP1 Expression in BOEC and Oviduct: An Immunohistochemicaland Immunocytochemical Study. . 2021; 151 - 158. 10.9775/kvfd.2020.24902
IEEE	KURUM A,Kocamis H,Karahan S,Türk M,OZKABADAYI Y "OVGP1 Expression in BOEC and Oviduct: An Immunohistochemicaland Immunocytochemical Study." , ss.151 - 158, 2021. 10.9775/kvfd.2020.24902
ISNAD	KURUM, AYTUL vd. "OVGP1 Expression in BOEC and Oviduct: An Immunohistochemicaland Immunocytochemical Study". (2021), 151-158. https://doi.org/10.9775/kvfd.2020.24902

APA	KURUM A, Kocamis H, Karahan S, Türk M, OZKABADAYI Y (2021). OVGP1 Expression in BOEC and Oviduct: An Immunohistochemicaland Immunocytochemical Study. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 27(2), 151 - 158. 10.9775/kvfd.2020.24902
Chicago	KURUM AYTUL,Kocamis Hakan,Karahan Siyami,Türk Mustafa,OZKABADAYI YASIN OVGP1 Expression in BOEC and Oviduct: An Immunohistochemicaland Immunocytochemical Study. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 27, no.2 (2021): 151 - 158. 10.9775/kvfd.2020.24902
MLA	KURUM AYTUL,Kocamis Hakan,Karahan Siyami,Türk Mustafa,OZKABADAYI YASIN OVGP1 Expression in BOEC and Oviduct: An Immunohistochemicaland Immunocytochemical Study. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.27, no.2, 2021, ss.151 - 158. 10.9775/kvfd.2020.24902
AMA	KURUM A,Kocamis H,Karahan S,Türk M,OZKABADAYI Y OVGP1 Expression in BOEC and Oviduct: An Immunohistochemicaland Immunocytochemical Study. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2021; 27(2): 151 - 158. 10.9775/kvfd.2020.24902
Vancouver	KURUM A,Kocamis H,Karahan S,Türk M,OZKABADAYI Y OVGP1 Expression in BOEC and Oviduct: An Immunohistochemicaland Immunocytochemical Study. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2021; 27(2): 151 - 158. 10.9775/kvfd.2020.24902
IEEE	KURUM A,Kocamis H,Karahan S,Türk M,OZKABADAYI Y "OVGP1 Expression in BOEC and Oviduct: An Immunohistochemicaland Immunocytochemical Study." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 27, ss.151 - 158, 2021. 10.9775/kvfd.2020.24902
ISNAD	KURUM, AYTUL vd. "OVGP1 Expression in BOEC and Oviduct: An Immunohistochemicaland Immunocytochemical Study". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 27/2 (2021), 151-158. https://doi.org/10.9775/kvfd.2020.24902