The Effect of Epigallocatechin-3-Gallate on Transcriptional Regulation of Migration and Inflammation Related Genes in Benign Prostate Hyperplasia Cells
Yıl: 2021 Cilt: 8 Sayı: 2 Sayfa Aralığı: 323 - 330 Metin Dili: İngilizce DOI: 10.34087/cbusbed. 831854 İndeks Tarihi: 02-10-2021
The Effect of Epigallocatechin-3-Gallate on Transcriptional Regulation of Migration and Inflammation Related Genes in Benign Prostate Hyperplasia Cells
Öz: Objective: This study was aimed to evaluate the role of epigallocatechin-3-gallate (EGCG) in the transcriptionalregulation of genes associated with migration and inflammation in benign prostate hyperplasia (BPH-1) cells.Materials and Methods: Effect of EGCG treatment on expressions of FAK, PXN, RhoA, Rac1, Cdc42, PAK1,ROCK1, WASL genes related to migration and IL-8, IL-6, NFκB p50, NFκB p65, IκBα genes related to inflammationwere determined by quantitative real time-polymerase chain reaction (qRT-PCR).Results: It was determined that EGCG treatment did not significantly change the expressions of investigated genesover 2 fold in terms of mRNA levels. EGCG, which effectively suppresses protein phosphorylations and levels, doesnot play a role in transcriptional regulation of migration and inflammation-related genes. These results show thatEGCG probably reduces the activity of FAK and NFκB signaling pathways by altering the protein function withoutaffecting mRNA levels.Conclusion: It is thought that EGCG may be useful in the treatment of premalignant lesions such as LUTS (lowerurinary tract symptoms) and BPH, and its mechanism of action can be predominantly realized at post-translationallevel.
Anahtar Kelime: Epigallokateşin-3-gallat'ın Benign Prostat Hiperplazi Hücrelerinde Migrasyon ve Enflamasyon ile İlişkili Genlerin Transkripsiyonel Regülasyonuna Etkisi
Öz: Giriş ve Amaç: Bu çalışmada epigallokateşin-3-gallat’ın (EGCG) benign prostat hiperplazisi hücrelerinde migrasyon ve inflamasyon ile ilişkili genlerin transkripsiyonel regülasyonundaki rolünün araştırılması amaçlanmıştır. Gereç ve Yöntemler: EGCG uygulamasının migrasyon ile ilişkili FAK, PXN, RhoA, Rac1, Cdc42, PAK1, ROCK1, WASL genlerinin ve inflamasyon ile ilişkili IL-8, IL-6, NFκB p50, NFκB p65, IκBα genlerinin ekspresyonları üzerine etkisi kantitatif gerçek zamanlı-polimeraz zincir reaksiyonu (qRT-PCR) kullanılarak tespit edildi. Bulgular: EGCG uygulamasının araştırılan genlerin mRNA seviyelerinde 2 kattan fazla anlamlı bir değişikliğe yol açmadığı belirlendi. Protein fosforilasyonlarını ve seviyelerini etkili bir şekilde baskılayan EGCG’nin, migrasyon ve inflamasyon ile ilişkili genlerin transkripsiyonel regülasyonunda rol oynamadığı belirlenmiştir. Elde edilen sonuçlar, EGCG’nin büyük olasılıkla mRNA seviyelerini etkilemeden protein fonksiyonunu değiştirerek FAK ve NFκB sinyal yolaklarınının aktivitesini azalttığını göstermektedir. Sonuç: EGCG’nin alt idrar yolu semptomları ve BPH gibi premalign lezyonların tedavisinde faydalı olabileceği ve etki mekanizmasının baskın olarak post-translasyonel seviyede gerçekleştiği düşünülmektedir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- 1.Ke, Z.B, Cai, H, et al., Identification of key genes and pathways in benign prostatic hyperplasia, Journal of Cellular Physiology, 2019, 234(11), 19942-19950.
- 2.Zhou, J, Lei, Y, Chen, J, Zhou, X, Potential ameliorative effects of epigallocatechin-gallate against testosterone-induced benign prostatic hyperplasia and fibrosis in rats, International Immunopharmacology, 2018, 64, 162-169.
- 3.Penna, G, Fibbi, B, et al., The Vitamin D Receptor Agonist Elocalcitol Inhibits IL-8-Dependent Benign Prostatic Hyperplasia Stromal Cell Proliferation and Inflammatory Response by Targeting the RhoA/RhoKinase and NF-kB Pathways, The Prostate, 2009, 69, 480-493.
- 4.Iglesias-Gato, D, Carsten, T, et al., Androgen-independent effects of Serenoa repens extract (Prostasan®) on prostatic epithelial cell proliferation and inflammation, Phytotherapy Research: PTR, 2012, 26(2), 259-264.
- 5.Eleazu, C, Eleazu, K, Kalu, W, Management of Benign Prostatic Hyperplasia: could dietary polyphenols be an alternative to existing therapies?, Frontiers and Pharmacology, 2017, 8(234), 1-11.
- 6.Briganti, A, Capitanio, U, et al., Benign prostatic hyperplasia and its etiologies, European Urology Supplements, 2009, 8, 865-71.
- 7.De Nunzio, C, Kramer, G, et al., The controversial relationship between benign prostatic hyperplasia and prostate cancer: the role of inflammation, European Urology, 2011, 60, 106–117.
- 8.Untergasser, G, Madersbacher, S, Berger, P, Benign prostatic hyperplasia: age-related tissue-remodeling, Experimental Gerontology, 2005, 40, 121–128.
- 9.Roberts, R.O, Jacobson, D.J, Rhodes, T, Klee, G.G, Leiber, M.M, Jacobsen, S.J, Serum sex hormones and measures of benign prostatic hyperplasia, Prostate, 2004, 61(2), 124-131.
- 10. Giri, D, Ittmann, M. Interleukin-8 is a paracrine inducer of fibroblast growth factor 2, a stromal and epithelial growth factor in benign prostatic hyperplasia, The American Journal of Pathology, 2001, 159, 139-147.
- 11. Chughtai, B, Lee, R, Te, A, Kaplan, S, Role of Inflammation in Benign Prostatic Hyperplasia, Reviews in Urology, 2011, 13, 147- 150.
- 12. Minciullo, P.L, Inferrera, A, Navarra, M, Calapai, G, Magno, C, Gangemi, S, Oxidative stress in benign prostatic hyperplasia: a systematic review, Urologia Internationalis, 2015, 94(3), 249–254.
- 13. Wang, L, Yang, J.R, Yang, L.Y, Liu, Z.T, Chronic inflammation in benign prostatic hyperplasia: implications for therapy, Medical Hypotheses, 2008, 70(5), 1021-1023.
- 14. Erbaykent Tepedelen, B, Soya, E, Korkmaz, M, Epigallocatechin-3- gallate reduces the proliferation of benign prostatic hyperplasia cells via regulation of focal adhesions, Life Sciences, 2017, 191, 74-81.
- 15. Sarbishegi, M, Khani, M, Salimi, S, Valizadeh, M, Sargolzaei Aval, F, Antiproliferative and antioxidant effects of Withania coagulans extract on benign prostatic hyperplasia in rats, Nephro-Urology Monthly, 2016, 8(1), e33180.
- 16. Grant, P, Ramasamy, S. An update on plant derived anti-androgens, International Journal of Endocrinology and Metabolism, 2012, 10(2), 497-502.
- 17. Hiipakka, R.A, Zhang, H.Z, Dai, W, Dai, Q, Liao, S, Structureactivity relationships for inhibition of human 5α-reductases by polyphenols, Biochemical Pharmacology, 2002, 63, 1165-1176.
- 18. Negri, A, Naponelli, V, Rizzi, F, Bettuzzi, S, Molecular Targets of Epigallocatechin-Gallate (EGCG): A Special Focus on Signal Transduction and Cancer, Nutrients, 2018, 10(12), 1936.
- 19. Singh, B.N, Shankar, S, Srivastava, R.K, Green tea catechin, epigallocatechin-3-gallate (EGCG): mechanisms, perspectives and clinical applications, Biochemical Pharmacology, 2011, 82(12), 1807-1821.
- 20. Luo, X, Guo, L, Zhang, L, Hu, Y, Shang, D, Ji, D, Bioinformatics analysis of microarray profiling identifies the mechanism of focal adhesion kinase signalling pathway in proliferation and apoptosis of breast cancer cells modulated by green tea polyphenol epigallocatechin 3-gallate. The Journal of Pharmacy and Pharmacology, 2018, 70(12), 1606–1618.
- 21. Sen, T, Dutta, A, Chatterjee, A, Epigallocatechin-3-gallate (EGCG) downregulates gelatinase-B (MMP-9) by involvement of FAK/ERK/NFkappaB and AP-1 in the human breast cancer cell line MDA-MB-231, Anti-cancer Drugs, 2010, 21(6), 632–644. 22. Khan, N, Afaq, F, Saleem, M, Ahmad, N, Mukhtar, H, Targeting multiple signaling pathways by green tea polyphenol (-)- epigallocatechin-3-gallate, Cancer Research, 2006, 66(5), 2500– 2505.
- 23. Bettuzzi, S, Brausi, M, Rizzi, F, Castagnetti, G, Peracchia, G, Corti, A, Chemoprevention of human prostate cancer by oral administration of green tea catechins in volunteers with high-grade prostate intraepithelial neoplasia: a preliminary report from a one-year proofof-principle study, Cancer Research, 2006, 66(2), 1234–1240.
- 24. Chen, J, Song, H, Protective potential of epigallocatechin-3-gallate against benign prostatic hyperplasia in metabolic syndrome rats, Environmental Toxicology and Pharmacology, 2016, 45, 315–320. 25. Ganguly, K.K, Sen, T, Pal, S, Biswas, J, Chatterjee, A, Studies on focal adhesion kinase in human breast cancer cell MDA-MB-231, Advances in Biological Chemistry, 2012, 2, 29–42.
- 26. Chan, C.M, Huang, J.H, Chiang, H.S, Wu, W.B, Lin, H.H, Hong, J.Y, Hung, C.F, Effects of (−)-epigallocatechin gallate on RPE cell migration and adhesion, Molecular Vision, 2010, 16, 586-595.
- 27. Sajadimajd, S, Bahramsoltani, R, et al., Advances on Natural Polyphenols as Anticancer Agents for Skin Cancer, Pharmacological Research, 2020, 151, 104584.
- 28. Zhang, C.H, Wang, J.X, Cai, M.L, Shao, R, Liu, H, Zhao, W.L, The roles and mechanisms of G3BP1 in tumour promotion, Journal of Drug Targeting, 2019, 27(3), 300-305.
- 29. Hwang, Y.S, Park, K.K, Chung, W.Y, Epigallocatechin-3 gallate inhibits cancer invasion by repressing functional invadopodia formation in oral squamous cell carcinoma, European Journal of Pharmacology, 2013, 715, 286-295.
- 30. Tsujimura, A, Fukuhara, S, et al., Histologic evaluation of human benign prostatic hyperplasia treated by dutasteride: a study by xenograft model with improved severe combined immunodeficient mice, Urology, 2015, 85(1), 274.e1–274.e8.
- 31. Vu, H.A, Beppu, Y, et al., Green tea epigallocatechin gallate exhibits anticancer effect in human pancreatic carcinoma cells via the inhibition of both focal adhesion kinase and insulin-like growth factor-I receptor, Journal of Biomedicine & Biotechnology, 2010, 2010, 290516.
- 32. Wang, S.I, Mukhtar, H, Gene expression profile in human prostate LNCaP cancer cells by (2) epigallocatechin-3-gallate, Cancer Letters, 2002, 182, 43–51. 33. Okabe, S, Fujimoto, N, Sueoka, N, Suganuma, M, Fujiki, H, Modulation of gene expression by (–)-epigallocatechin gallate in PC9 cells using a cDNA expression array, Biological and Pharmaceutical Bulletin, 2001, 24(8), 883–886.
- 34. Ahn, W.S, Huh, S.W, et al., A major constituent of green tea, EGCG, inhibits the growth of a human cervical cancer cell line, CaSki cells, through apoptosis, G (1) arrest, and regulation of gene expression, DNA Cell Biology, 2003, 22(3), 217–224.
- 35. Sartipp our, M.R, Heber, D, et al., cDNA microarray analysis of endothelial cells in response to green tea reveals a suppressive phenotype, International Journal of Oncology, 2004, 25(1), 193-202.
- 36. Guo, S, Yang, S, Taylor, C, Sonenshein, G.E, Green tea polyphenol epigallocatechin-3 gallate (EGCG) affects gene expression of breast cancer cells transformed by the carcinogen 7,12- dimethylbenz[a]anthracene, The Journal of Nutrition, 2005, 135, 2978S–2986S.
- 37. Murphy, J.M, Jeong, K, et al., FAK and Pyk2 activity promote TNFα and IL-1β-mediated pro-inflammatory gene expression and vascular inflammation, Scientific Reports, 2019, 9, 7617.
- 38. Hoffmann, E, Breiholz, O.D, Holtmann, H, Kracht, M, Multiple control of interleukin-8 gene expression, Journal of Leukocyte Biology, 2002, 72, 847-855.
- 39. Hsu, A, Bruno, R.S, et al., Dietary soy and tea mitigate chronic inflammation and prostate cancer via NFκB pathway in the Noble rat model, The Journal of Nutritional Biochemistry, 2011, 22(5), 502- 510.
- 40. Baloğlu M, Özkorkmaz E.G, Menisküs Yırtığı Hastalarının Sinoviyal Hücrelerinde Matriks Metalloprotein-2 ve NFκB Protein Ekspresyonu, Celal Bayar University-Health Science Institute Journal, 2019, 6(4), 209-214.
- 41. Cicero, A.F, Allkanjari, O, et al., Nutraceutical treatment and prevention of benign prostatic hyperplasia and prostate cancer, Archivio Italiano di Urologia e Andrologia, 2019, 91(3), 139-152.
| APA | Erbaykent Tepedelen B (2021). The Effect of Epigallocatechin-3-Gallate on Transcriptional Regulation of Migration and Inflammation Related Genes in Benign Prostate Hyperplasia Cells. , 323 - 330. 10.34087/cbusbed. 831854 |
| Chicago | Erbaykent Tepedelen Burcu The Effect of Epigallocatechin-3-Gallate on Transcriptional Regulation of Migration and Inflammation Related Genes in Benign Prostate Hyperplasia Cells. (2021): 323 - 330. 10.34087/cbusbed. 831854 |
| MLA | Erbaykent Tepedelen Burcu The Effect of Epigallocatechin-3-Gallate on Transcriptional Regulation of Migration and Inflammation Related Genes in Benign Prostate Hyperplasia Cells. , 2021, ss.323 - 330. 10.34087/cbusbed. 831854 |
| AMA | Erbaykent Tepedelen B The Effect of Epigallocatechin-3-Gallate on Transcriptional Regulation of Migration and Inflammation Related Genes in Benign Prostate Hyperplasia Cells. . 2021; 323 - 330. 10.34087/cbusbed. 831854 |
| Vancouver | Erbaykent Tepedelen B The Effect of Epigallocatechin-3-Gallate on Transcriptional Regulation of Migration and Inflammation Related Genes in Benign Prostate Hyperplasia Cells. . 2021; 323 - 330. 10.34087/cbusbed. 831854 |
| IEEE | Erbaykent Tepedelen B "The Effect of Epigallocatechin-3-Gallate on Transcriptional Regulation of Migration and Inflammation Related Genes in Benign Prostate Hyperplasia Cells." , ss.323 - 330, 2021. 10.34087/cbusbed. 831854 |
| ISNAD | Erbaykent Tepedelen, Burcu. "The Effect of Epigallocatechin-3-Gallate on Transcriptional Regulation of Migration and Inflammation Related Genes in Benign Prostate Hyperplasia Cells". (2021), 323-330. https://doi.org/10.34087/cbusbed. 831854 |
| APA | Erbaykent Tepedelen B (2021). The Effect of Epigallocatechin-3-Gallate on Transcriptional Regulation of Migration and Inflammation Related Genes in Benign Prostate Hyperplasia Cells. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi, 8(2), 323 - 330. 10.34087/cbusbed. 831854 |
| Chicago | Erbaykent Tepedelen Burcu The Effect of Epigallocatechin-3-Gallate on Transcriptional Regulation of Migration and Inflammation Related Genes in Benign Prostate Hyperplasia Cells. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi 8, no.2 (2021): 323 - 330. 10.34087/cbusbed. 831854 |
| MLA | Erbaykent Tepedelen Burcu The Effect of Epigallocatechin-3-Gallate on Transcriptional Regulation of Migration and Inflammation Related Genes in Benign Prostate Hyperplasia Cells. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi, vol.8, no.2, 2021, ss.323 - 330. 10.34087/cbusbed. 831854 |
| AMA | Erbaykent Tepedelen B The Effect of Epigallocatechin-3-Gallate on Transcriptional Regulation of Migration and Inflammation Related Genes in Benign Prostate Hyperplasia Cells. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi. 2021; 8(2): 323 - 330. 10.34087/cbusbed. 831854 |
| Vancouver | Erbaykent Tepedelen B The Effect of Epigallocatechin-3-Gallate on Transcriptional Regulation of Migration and Inflammation Related Genes in Benign Prostate Hyperplasia Cells. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi. 2021; 8(2): 323 - 330. 10.34087/cbusbed. 831854 |
| IEEE | Erbaykent Tepedelen B "The Effect of Epigallocatechin-3-Gallate on Transcriptional Regulation of Migration and Inflammation Related Genes in Benign Prostate Hyperplasia Cells." Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi, 8, ss.323 - 330, 2021. 10.34087/cbusbed. 831854 |
| ISNAD | Erbaykent Tepedelen, Burcu. "The Effect of Epigallocatechin-3-Gallate on Transcriptional Regulation of Migration and Inflammation Related Genes in Benign Prostate Hyperplasia Cells". Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi 8/2 (2021), 323-330. https://doi.org/10.34087/cbusbed. 831854 |
