Determination of MIC Values of Various Antimicrobial Agents andPresence of Resistance Genes in Pasteurella multocida StrainsIsolated from Bovine

öztürk, cihat; EKIN, Ismail Hakki; GULAYDIN, Ozgul; Gürtürk, Kemal

doi:10.9775/kvfd.2020.25093

Determination of MIC Values of Various Antimicrobial Agents andPresence of Resistance Genes in Pasteurella multocida StrainsIsolated from Bovine

Özgül GÜLAYDIN, (Van Yüzüncü Yıl Üniversitesi, Veteriner Fakültesi, Mikrobiyoloji Anabilim Dalı, Van, Türkiye)

Kemal GÜRTÜRK, (Van Yüzüncü Yıl Üniversitesi, Veteriner Fakültesi, Mikrobiyoloji Anabilim Dalı, Van, Türkiye)

Ismail Hakki EKİN, (Van Yüzüncü Yıl Üniversitesi, Veteriner Fakültesi, Mikrobiyoloji Anabilim Dalı, Van, Türkiye)

Cihat ÖZTÜRK (Van Yüzüncü Yıl Üniversitesi, Veteriner Fakültesi, Mikrobiyoloji Anabilim Dalı, Van, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

1 0

Yıl: 2021 Cilt: 27 Sayı: 2 Sayfa Aralığı: 217 - 224 Metin Dili: İngilizce DOI: 10.9775/kvfd.2020.25093 İndeks Tarihi: 03-10-2021

Determination of MIC Values of Various Antimicrobial Agents andPresence of Resistance Genes in Pasteurella multocida StrainsIsolated from Bovine

Öz:

Pasteurella multocida is an important bacterium that can cause respiratory infections in cattle. Due to the usage of antimicrobial agents in the treatment of the disease frequently, it is critical to follow the antimicrobial susceptibility of the isolates. In this study, minimal inhibitory concentrations (MIC) of various antimicrobial agents and presence of genes related to resistance were investigated in 59 P. multocida strains isolated from the respiratory tract of cattle. According to MIC values determined by E-test, all of the isolates were susceptible to enrofl oxacin, chloramphenicol and gentamicin, but resistant to cefoxitin. In addition, high resistance to ampicillin (88.14%), tilmicosin (64.41%), clindamycin (83.05%) and streptomycin (59.32%) were observed in the isolates. When the resistance genes were examined by PCR, it was determined that blaROB-1, tet H, sul II, str A/aphA 1 and erm 42 genes could play an important role in penicillin, tetracycline, sulfamethoxazole + trimethoprime, aminoglycoside and macrolide resistance, respectively. It was concluded that the usage of ampicillin, tetracycline, sulfamethoxazole + trimethoprime, macrolide and aminoglycosides should be considered for the treatment of respiratory tract infections caused by P. multocida in cattle. Also, it was determined that antimicrobial resistance genes could play an important role in the development of resistance in P. multocida.

Anahtar Kelime:

Sığırlardan İzole Edilen Pasteurella multocida Suşlarında Çeşitli Antimikrobiyal Maddelerin MİK Değerlerinin ve Antimikrobiyal Direnç Genlerinin Belirlenmesi

Öz:

Pasteurella multocida, sığırlarda solunum yolu enfeksiyonlarına neden olan önemli bir bakteriyel etkendir. Hastalığın tedavisinde sıklıklaantimikrobiyal tedavi uygulanması nedeniyle etkene yönelik antimikrobiyal duyarlılık sonuçlarının takip edilmesi kritik öneme sahiptir.Bu çalışmada, sığırların solunum yolundan izole edilen 59 adet P. multocida izolatında çeşitli antimikrobiyal maddelerin minimal inhibitörkonsantrasyonları (MİK) ve antimikrobiyal direnç ile ilişkili genlerin varlığı araştırıldı. E-test yöntemiyle belirlenen MİK değerlerine göre izolatlarıntamamı enrofl oxacin, chloramphenicol ve gentamicine duyarlı, cefoxitine ise dirençli bulundu. Ayrıca ampicillin (%88.14), tilmicosin (%64.41),clindamycin (%83.05) ve streptomycine (%59.32) yüksek oranda direnç tespit edildi. PCR ile antimikrobiyal direnç genlerinin varlığı incelendiğindeise penicillin, tetracycline, sulfamethoxazole + trimethoprime, aminoglikozid ve makrolid direncinde sırasıyla blaROB-1, tet H, sul II, str A/aphA 1 veerm 42 genlerinin önemli rol oynadığı belirlendi. Bu çalışmada, sığırlarda P. multocida suşlarının neden olduğu solunum yolu enfeksiyonlarınıntedavisinde ampicillin, tetracycline, sulfamethoxazole + trimethoprime ile makrolid ve aminoglikozid antibiyotiklerin kullanımına dikkat edilmesigerektiği sonucuna varıldı. Ayrıca, antimikrobiyal direnç ile ilişkili genlerin izolatlarda direnç gelişiminde önemli rol oynadığı belirlendi.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Horwood PF, Mahony TJ: Multiplex real-time RT-PCR detection of three viruses associated with the bovine respiratory disease complex. J Virol Methods, 171 (2): 360-363, 2011. DOI: 10.1016/j.jviromet.2010.11.020
2. Dziva F, Muhairwa AP, Bisgaard M, Christensen H: Diagnostic and typing options for investigating diseases associated with Pasteurella multocida. Vet Microbiol, 128 (1-2): 1-22, 2008. DOI: 10.1016/j.vetmic. 2007.10.018
3. Kehrenberg C, Schulze-Tanzil G, Martel JL, Chaslus-Dancla E, Schwarz S: Antimicrobial resistance in Pasteurella and Mannheimia: Epidemiology and genetic basis. Vet Res, 32 (3-4): 323-339, 2001. DOI: 10.1051/vetres:2001128
4. Sarangi LN, Thomas P, Gupta SK, Priyadarshini A, Kumar S, Nagaleekar VK, Kumar A, Singh VP: Virulence gene profiling and antibiotic resistance pattern of Indian isolates of Pasteurella multocida of small ruminant origin. Comp Immunol Microbiol Infect Dis, 38, 33-39, 2015. DOI: 10.1016/j. cimid.2014.11.003
5. Kehrenberg C, Catry B, Haesebrouck F, de Kruif A, Schwarz S: Novel spectinomycin/streptomycin resistance gene, aadA14, from Pasteurella multocida. Antimicrob Agents Chemother, 49 (7): 3046-3049, 2005. DOI: 10.1128/AAC.49.7.3046-3049.2005
6. Michael GB, Kadlec K, Sweeney MT, Brzuszkiewicz E, Liesegang H, Daniel R, Murray RW, Watts JL, Schwarz S: ICE Pmu1, an integrative conjugative element (ICE) of Pasteurella multocida: Analysis of the regions that comprise 12 antimicrobial resistance genes. J Antimicrob Chemother, 67 (1): 84-90, 2012. DOI: 10.1093/jac/dkr406
7. Eidam C, Poehlein A, Leimbach A, Michael GB, Kadlec K, Liesegang H, Daniel R, Sweeney MT, Murray RW, Watts JL, Schwarz S: Analysis and comparative genomics of ICEMh1, a novel integrative and conjugative element (ICE) of Mannheimia haemolytica. J Antimicrob Chemother, 70 (1): 93-97, 2015. DOI: 10.1093/jac/dku361
8. Wasteson Y, Roe DE, Falk K, Roberts MC: Characterization of tetracycline and erythromycin resistance in Actinobacillus pleuropneumoniae. Vet Microbiol, 48, 41-50, 1996. DOI: 10.1016/0378-1135(95)00130-1
9. Desmolaize B, Rose S, Wilhelm C, Warrass R, Douthwaite S: Combinations of macrolide resistance determinants in field isolates of Mannheimia haemolytica and Pasteurella multocida. Antimicrob Agents Chemother, 55 (9): 4128-4133, 2011. DOI: 10.1128/AAC.00450-11
10. Rose S, Desmolaize B, Jaju P, Wilhelm C, Warrass R, Douthwaite S: Multiplex PCR to identify macrolide resistance determinants in Mannheimia haemolytica and Pasteurella multocida. Antimicrob Agents Chemother, 56 (7): 3664-3669, 2012. DOI: 10.1128/AAC.00266-12
11. Blanco M, Gutiérrez-Martin CB, Rodríguez-Ferri EF, Roberts MC, Navas J: Distribution of tetracycline resistance genes in Actinobacillus pleuropneumoniae isolates from Spain. Antimicrob Agents Chemother, 50 (2): 702-708, 2006. DOI: 10.1128/AAC.50.2.702-708.2006
12. Livrelli VO, Darfeuille-Richaud A, Rich CD, Joly BH, Martel JL: Genetic determinant of the ROB-1 beta-lactamase in bovine and porcine Pasteurella strains. Antimicrob Agents Chemother, 32 (8): 1282-1284, 1988. DOI: 10.1128/AAC.32.8.1282
13. Onat K, Kahya S, Çarli KT: Frequency and antibiotic susceptibility of Pasteurella multocida and Mannheimia haemolytica isolates from nasal cavities of cattle. Turk J Vet Anim Sci, 34 (1): 91-94, 2010. DOI: 10.3906/ vet-0901-1
14. Ulker H, Kucuk D, Cantekin Z, Solmaz H: Hatay yöresinde kesimhanede kesilen sığır akciğerlerinden Pasteurella multocida ve Mannheimiahaemolytica izolasyonu ve antibiyotiklere duyarlılığı. AVKAE Derg, 2 (2): 10-14, 2012.
15. Guler L, Gunduz K, Sarisahin AS: Capsular typing and antimicrobial susceptibility of Pasteurella multocida isolated from different hosts. Kafkas Univ Vet Fak Derg, 19 (5): 843-849, 2013. DOI: 10.9775/kvfd.2013.8936
16. Quinn PJ, Markey BK, Leonard FC, Fitzpatrick ES, Fanning S, Hartigan PJ: Pasteurella species, Mannheima haemolytica and Bibersteinia trehalosi. In, Veterinary Microbiology and Microbial Disease. 2nd ed., 300- 308, John Wiley & Sons Ltd., UK, 2011.
17. Townsend KM, Frost AJ, Lee CW, Papadimitriou JM, Dawkins HJS: Development of PCR assays for species and type-specific identification of Pasteurella multocida isolates. J Clin Microbiol, 36 (4): 1096-1100, 1998.
18. European Committee on Antimicrobial Susceptibility Testing: Clinical Breakpoint Tables v. 9.0, valid from 2019-01-01.
19. Clinical Laboratory Standart Institute: Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard. 2nd ed., Wayne, PA: CLSI, 2002.
20. Clinical Laboratory Standart Institute: Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated From Animals. 4th ed., Vet 08. Wayne, PA: CLSI, 2018.
21. Matter D, Rossano A, Limat S, Vorlet-Fawer L, Brodard I, Perreten V: Antimicrobial resistance profile of Actinobacillus pleuropneumoniae and Actinobacillus porcitonsillarum. Vet Microbiol, 122 (1-2): 146-156, 2007. DOI: 10.1016/j.vetmic.2007.01.009
22. Kehrenberg C, Schwarz S: Occurrence and linkage of genes coding for resistance to sulfonamides, streptomycin and chloramphenicol in bacteria of the genera Pasteurella and Mannheimia. FEMS Microbiol Lett, 205, 283-290, 2001. DOI: 10.1111/j.1574-6968.2001.tb10962.x
23. Maynard C, Fairbrother JM, Bekal S, Sanschagrin F, Levesque RC, Brousseau R, Maason L, Lariviere S, Harel J: Antimicrobial resistance enes in enterotoxigenic Escherichia coli O149: K91 isolates obtained over a 23 year period from pigs. Antimicrob Agents Chemother, 47 (10): 3214- 3221, 2003. DOI: 10.1128/aac.47.10.3214-3221.2003
24. Akinbowale OL, Peng H, Barton MD: Diversity of tetracycline resistance genes in bacteria from aquaculture sources in Australia. J Appl Microbiol, 103 (5): 2016-2025, 2007. DOI: 10.1111/J.1365-2672.2007.03445.X
25. Wang Z, Kong LC, Jia BY, Liu SM, Jiang XY, Ma HX: Aminoglycoside susceptibility of Pasteurella multocida isolates from bovine respiratory infections in China and mutations in ribosomal protein S5 associated with high-level induced spectinomycin resistance. J Vet Med Sci, 79 (10): 1678- 1681, 2017. DOI: 10.1292/jvms.17-0219
26. Benedict KM, Gow SP, Checkley S, Booker CW, McAllister TA, Morley PS: Methodological comparisons for antimicrobial resistance surveillance in feedlot cattle. BMC Vet Res, 9:216, 2013. DOI: 10.1186/ 1746-6148-9-216
27. Yoshimura H, Ishimaru M, Endoh YS, Kojima A: Antimicrobial susceptibility of Pasteurella multocida isolated from cattle and pigs. J Vet Med B, 48 (7): 555-560, 2001. DOI: 10.1111/j.1439-0450.2001.00468.x
28. Garch F, de Jong A, Simjee S, Moyaert H, Klein U, Ludwig C, Marion H, Haag-Diergarten S, Richard-Mazer A, Thomas V, Siegwart E: Monitoring of antimicrobial susceptibility of respiratory tract pathogens isolated from diseased cattle and pigs across Europe, 2009-2012: VetPath results. Vet Microbiol, 194, 11-22, 2016. DOI: 10.1016/j.vetmic.2016.04.009
29. Anholt RM, Klima C, Allan N, Matheson-Bird H, Schatz C, Ajitkumar P, Otto SJG, Peters D, Schmid K, Olson M, McAllister T, Ralston B: Antimicrobial susceptibility of bacteria that cause bovine respiratory disease complex in Alberta, Canada. Front Vet Sci, 4:207, 2017. DOI: 10.3389/fvets.2017.00207
30. Katsuda K, Hoshinoo K, Ueno Y, Kohmoto M, Mikami O: Virulence genes and antimicrobial susceptibility in Pasteurella multocida isolates from calves. Vet Microbiol, 167 (3-4): 737-741, 2013. DOI: 10.1016/j.vetmic. 2013.09.029
31. Dayao DAE, Gibson JS, Blackall PJ, Turni C: Antimicrobial resistance genes in Actinobacillus pleuropneumoniae, Haemophilus parasuis and Pasteurella multocida isolated from Australian pigs. Aust Vet J, 94 (7): 227- 231, 2016. DOI: 10.1111/avj.12458
32. Cucco L, Massacci FR, Sebastiani C, Mangili P, Bano L, Cocchi M, Luppi A, Ortenzi R, Pezzotti G, Magistrali CF: Molecular characterization and antimicrobial susceptibility of Pasteurella multocida strains isolated from hosts affected by various diseases in Italy. Vet Ital, 53 (1): 21-27, 2017. DOI: 10.12834/VetIt.661.3256.2
33. Timsit E, Hallewell J, Booker C, Tison N, Amat S, Alexander TW: Prevalence and antimicrobial susceptibility of Mannheimia haemolytica, Pasteurella multocida, and Histophilus somni isolated from the lower respiratory tract of healthy feedlot cattle and those diagnosed with bovine respiratory disease. Vet Microbiol, 208, 118-125, 2017. DOI: 10.1016/j.vetmic.2017.07.013
34. Kadlec K, Michael GB, Sweeney MT, Brzuszkiewicz E, Liesegang H, Daniel R, Watts JL, Schwarz S: Molecular basis of macrolide, triamilide, and lincosamide resistance in Pasteurella multocida from bovine respiratory disease. Antimicrob Agents Chemother, 55 (5): 2475-2477, 2011. DOI: 10.1128/AAC.00092-11

APA	GULAYDIN O, Gürtürk K, EKIN I, öztürk c (2021). Determination of MIC Values of Various Antimicrobial Agents andPresence of Resistance Genes in Pasteurella multocida StrainsIsolated from Bovine. , 217 - 224. 10.9775/kvfd.2020.25093
Chicago	GULAYDIN Ozgul,Gürtürk Kemal,EKIN Ismail Hakki,öztürk cihat Determination of MIC Values of Various Antimicrobial Agents andPresence of Resistance Genes in Pasteurella multocida StrainsIsolated from Bovine. (2021): 217 - 224. 10.9775/kvfd.2020.25093
MLA	GULAYDIN Ozgul,Gürtürk Kemal,EKIN Ismail Hakki,öztürk cihat Determination of MIC Values of Various Antimicrobial Agents andPresence of Resistance Genes in Pasteurella multocida StrainsIsolated from Bovine. , 2021, ss.217 - 224. 10.9775/kvfd.2020.25093
AMA	GULAYDIN O,Gürtürk K,EKIN I,öztürk c Determination of MIC Values of Various Antimicrobial Agents andPresence of Resistance Genes in Pasteurella multocida StrainsIsolated from Bovine. . 2021; 217 - 224. 10.9775/kvfd.2020.25093
Vancouver	GULAYDIN O,Gürtürk K,EKIN I,öztürk c Determination of MIC Values of Various Antimicrobial Agents andPresence of Resistance Genes in Pasteurella multocida StrainsIsolated from Bovine. . 2021; 217 - 224. 10.9775/kvfd.2020.25093
IEEE	GULAYDIN O,Gürtürk K,EKIN I,öztürk c "Determination of MIC Values of Various Antimicrobial Agents andPresence of Resistance Genes in Pasteurella multocida StrainsIsolated from Bovine." , ss.217 - 224, 2021. 10.9775/kvfd.2020.25093
ISNAD	GULAYDIN, Ozgul vd. "Determination of MIC Values of Various Antimicrobial Agents andPresence of Resistance Genes in Pasteurella multocida StrainsIsolated from Bovine". (2021), 217-224. https://doi.org/10.9775/kvfd.2020.25093

APA	GULAYDIN O, Gürtürk K, EKIN I, öztürk c (2021). Determination of MIC Values of Various Antimicrobial Agents andPresence of Resistance Genes in Pasteurella multocida StrainsIsolated from Bovine. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 27(2), 217 - 224. 10.9775/kvfd.2020.25093
Chicago	GULAYDIN Ozgul,Gürtürk Kemal,EKIN Ismail Hakki,öztürk cihat Determination of MIC Values of Various Antimicrobial Agents andPresence of Resistance Genes in Pasteurella multocida StrainsIsolated from Bovine. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 27, no.2 (2021): 217 - 224. 10.9775/kvfd.2020.25093
MLA	GULAYDIN Ozgul,Gürtürk Kemal,EKIN Ismail Hakki,öztürk cihat Determination of MIC Values of Various Antimicrobial Agents andPresence of Resistance Genes in Pasteurella multocida StrainsIsolated from Bovine. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.27, no.2, 2021, ss.217 - 224. 10.9775/kvfd.2020.25093
AMA	GULAYDIN O,Gürtürk K,EKIN I,öztürk c Determination of MIC Values of Various Antimicrobial Agents andPresence of Resistance Genes in Pasteurella multocida StrainsIsolated from Bovine. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2021; 27(2): 217 - 224. 10.9775/kvfd.2020.25093
Vancouver	GULAYDIN O,Gürtürk K,EKIN I,öztürk c Determination of MIC Values of Various Antimicrobial Agents andPresence of Resistance Genes in Pasteurella multocida StrainsIsolated from Bovine. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2021; 27(2): 217 - 224. 10.9775/kvfd.2020.25093
IEEE	GULAYDIN O,Gürtürk K,EKIN I,öztürk c "Determination of MIC Values of Various Antimicrobial Agents andPresence of Resistance Genes in Pasteurella multocida StrainsIsolated from Bovine." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 27, ss.217 - 224, 2021. 10.9775/kvfd.2020.25093
ISNAD	GULAYDIN, Ozgul vd. "Determination of MIC Values of Various Antimicrobial Agents andPresence of Resistance Genes in Pasteurella multocida StrainsIsolated from Bovine". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 27/2 (2021), 217-224. https://doi.org/10.9775/kvfd.2020.25093